Monika Karaś

• PhD
• University of Life Sciences in Lublin

Sustainability in bio-based edible films, coatings, and packaging integrates environmental, economic, and social sustainability using renewable resources. These materials offer an eco-friendly alternative to traditional petroleum-based plastics and can extend the shelf life of fruits. The wine industry's by-products, rich in bioactive compounds, ca...

The aim of this study was to compare the effect of increasing concentrations (0, 1, 2, 4%) of sea buckthorn oil (SBO) on the structural, physicochemical, release, and antioxidant properties of glycerol-plasticized sodium casein (NaCAS) and gelatin (GEL) films. Ultrasonic treatment ensured effective homogenization of SBO in both types of emulsions,...

The aim of this study was to compare the effect of increasing concentrations (0, 1, 2, 4%) of sea buckthorn oil (SBO) on the structural, physicochemical, release, and antioxidant properties of glycerol-plasticized sodi-um casein (NaCAS) and gelatin (GEL) films. Ultrasonic treatment ensured effective homogenization of SBO in both types of emulsions,...

This study investigated the impact of adding raspberry pomace to the phenolic content and biological properties of freeze-dried apple/raspberry bars. The bars were prepared by replacing apple puree with raspberry pomace (5–50%), and their phenolic compounds were assessed using ethanol and buffer extracts. This work also explored the potential bioac...

Edible flowers are a potential source of bioactive ingredients and are also an area of scientific research. Particularly noteworthy are Cyani flos, which have a wide range of uses in herbal medicine. The below study aimed to investigate the influence of selected soluble fiber fractions on the selected properties of physical and biochemical powders...

In this study, our research aim was to assess the influence of pumpkin seed flour addition on the antioxidant properties, consumer acceptability, functional properties, and texture of wafers. The in vitro gastrointestinal digestion process was used to assess the effectiveness of fortification in terms of the potential bioavailability of phenolic co...

The almond tree (Prunus dulcis) is one of the most popular dry fruit trees, occupying first place in worldwide production. However, several by-products are generated during their production, which have characteristics of interest but still need to be utilized, namely, the almond skins. Almond skin contains approximately 50-75% of the total phenols...

Almonds are one of the most produced nuts worldwide, and numerous studies have shown that they have nutritional and medicinal characteristics, which gives them the possibility of being applied in various products. However, several by-products are generated during their production, which have characteristics of interest but remain underutilised, nam...

Nisin (NIS) Z was incorporated (0.05 %, 0.1 %, 0.2 %) into edible films based on chitosan lactate (CHL) and 75/25 blends of polysaccharides (corn starch (CS), wheat starch (WS), oxidized potato starch (OPS), pullulan (PUL)) with CHL. The increase in the NIS/polymer ratio promoted the diffusion-driven release. Compared with the fully dissolvable CHL...

In this study, the effect of the addition of freeze-dried raspberry pomace on the content of phenolic compounds and the antioxidant and anti-inflammatory activity of wafers was investigated. Particular attention was paid to the biological activity of the potentially bioavailable fraction of polyphenols extracted via gastro-intestinal digestion. In...

The objective of this study was to determine of nutritional value, in vitro cytotoxicity, and oxidative stress parameters in cells of selected insect species (Tenebrio molitor and Zophobas morio) after 30 days of Styrofoam consumption. Furthermore, part of our research is also a consumer survey on the willingness to eat insects fed with Styrofoam (...

The aim of this study was to investigate the effect of raspberry juice addition on the content of phenolic compounds and the antioxidant activity of wafers. The research was carried out on non-supplemented wafers (control) and wafers in which water was replaced with raspberry juice in the amount of 10%, 20%, 50%, 75% and 100%. The potential bioavai...

The objective of this study was to examine the inhibition of the activity of enzymes associated with development of the metabolic syndrome by peptide fractions received from simulated gastrointestinal digestion and absorption of heat-treated edible insects. The inhibitory activities of insect-derived peptides were determined against key enzymes rel...

Generally, bioactive peptides are natural compounds of food or part of protein that are inactive in the precursor molecule. However, they may be active after hydrolysis and can be transported to the active site. Biologically active peptides can also be synthesized chemically and characterized. Peptides have many properties, including antihypertensi...

The aim of the study is to characterise biologically active hydolysates and peptide fractions obtained from vacuum-packed string beans (Phaseolus vulragis L.) (PB). Unpacked beans were a control sample. The influence on human squamous carcinoma cell line SCC-15 (ATCC CRL-1623) was determined. Packed bean (PB) and unpacked bean (UB) extracts were fo...

In the last decade, increasing attention has been focused on entomophagy in Europe. However, Western societies rarely experience insects as a food source. Depending on the country, the approach to entomophagy is variables. This study was performed to assess the perception of entomophagy in the Polish population and comparison with other European so...

In this study, the effect of increasing concentrations (0, 0.5, 1, 2% w/w) of potassium sorbate (KS) on the release and antimicrobial properties of edible films based on pullulan (PUL), gelatin (GEL) and their blends (75/25, 50/50, and 25/75) was investigated. The 75/25 and 50/50 polymer mixtures separated into GEL-rich microspheres. The biggest mi...

The aim of this study was to evaluate the biological properties, i.e. qualitative and quantitative characteristics of phenolic and flavonoid compounds, DPPH and ABTS+ scavenging and Fe2+ chelating activity, and the UV-protecting and cytotoxicity properties of Plantago lanceolata extracts. The assays included different extracts: aqueous (A), ethanol...

The influence of heat treatment (65°C and 100°C) on the activities of potential anti-inflammatory peptides obtained from a millet protein fraction was investigated. The molecular mass of proteins was found to be in the range of 6.5–100 kDa. The protein fractions were in vitro hydrolyzed in gastrointestinal-like conditions. The highest peptide conte...

The objective of this study was to analyze millet protein hydrolyzates and peptide fractions with molecular mass under 3.0 kDa obtained from grains treated with different temperature values as inhibitors of angiotensin-converting enzyme (ACE), α-amylase, and α-glucosidase activity. The protein fractions were hydrolyzed in vitro in gastrointestinal...

The influence of faba bean seed fermentation on the release of bioactive peptides after digestion in gastrointestinal conditions was investigated. The molecular mass of protein detected by SDS-PAGE was in the range from 6.5 to 97 kDa. The highest degree of protein hydrolysis (98.72%) was noted for protein obtained from seeds fermented for 3 h at 37...

It is commonly believed that gall inducers have the ability to control and program the host plant growth to their own benefit. The pattern of changes in the contents of reducing sugars, protein and phenolic compounds as well as the activity of chitinase and β-1,3-glucanase in galls, galled and intact leaves of Ulmus pumila were investigated during...

Food‐derived bioactive peptides can be released during digestion and have a potentially beneficial effect on human health. It is known that the penetration of peptides across the small intestine can take place in several ways. The research related to the bioavailability of peptides develops dynamically, but still raises many doubts. Since there are...

The aim of this study was to determine the effect of heat treatment of edible insects on antioxidant and anti‐inflammatory activities of peptide fractions from hydrolysates obtained by in vitro gastrointestinal digestion thereof. Identification of bioactive peptides from the edible insects and their chemical synthesis were carried out as well. The...

Garlic (Allium sativum L.) has a reputation as a therapeutic agent for many different diseases such as microbial infections, hypertension, hypercholesterolaemia, diabetes, atherosclerosis and cancer. Health benefits of garlic depend on its content of biologically-active compounds, which differs between cultivars and geographical regions. The aim of...

This study investigated the functional properties of three species of edible insects: Gryllodes sigillatus, Schistocerca gregaria, and Tenebrio molitor. The water and oil holding capacity, solubility, and foaming and emulsion properties were evaluated. The protein solubility showed minimum values at pH 5. The highest water and oil holding capacity...

The potential of insects as a source of protein for future food and feed is the object of numerous studies. The nutritional value of edible insects is well established, and other aspects of consumption thereof are investigated. In this chapter, we aim to summarize the main features of insects as food. We briefly describe the history of the usage of...

The purpose of this study was to compare the effects of increasing concentrations of ascorbate ions (AIs, 0–100 mM) in the form of ascorbic acid (AA) and sodium ascorbate (SA) on the properties of edible oxidized potato starch films. The browning reactions were faster in the SA-added films than in those of AA-added. In, turn, AA recrystallized fast...

Gall-making Cynipidae manipulate the leaves of host plant to form galls where offspring find shelter and food. The relationship between oak gallwasp and biochemical mechanisms of galls still requires a better understanding. So, in this research, protein and phenolic compound contents, as well as the activity of antioxidative enzymes and pathogenesi...

This study investigated the effect of heat treatment of edible insects on antioxidant and anti-inflammatory activities of peptides obtained by in vitro gastrointestinal digestion and absorption process thereof. The antioxidant potential of edible insect hydrolysates was determined as free radical-scavenging activity, ion chelating activity, and red...

The aim of this study was to determine the conditions of bean fermentation carried out by Lactobacillus plantarum to obtain biologically active peptide fractions after in vitro digestion. The results suggest that optimum process conditions should be selected according to the specific activity of peptides. Only a fraction with a molecular mass of 3....

There are many scientific reports on determination of the content and biological activity of compounds found in food. However, these analyses are not sufficient to determine their effect on the human body. During digestion of food ingredients, many changes can modify their structure and this may affect their absorption and bioactivity. Many phenoli...

This study investigated the antioxidant activities of peptides obtained by in vitro gastrointestinal digestion of edible insects. The antioxidant potential of edible insects' hydrolysates was determined as the free radical-scavenging activity, ion chelating activity and reducing power. The highest antiradical activity against DPPH˙, whose IC50 valu...

Background. Coffee is important source of natural antioxidants in the diet, such as phenolic compounds, alkaloids, mainly caffeine, diterpenes (cafestol and kahweol) and Maillard reaction products formed during roasting. Material and methods. In aqueous and methanolic extracts of coff ee (Coffea arabica L.) roasted using traditional techniques from...

Pietruszka zwyczajna (Petroselinum crispum Mill) jest wykorzystywana jako surowiec w przemyśle spożywczym m.in. ze względu na dużą zawartość związków fenolowych. W niniejszej pracy badano wpływ rozpuszczalników na skład oraz wybrane biologiczne właściwości ekstraktów z liści P. crispum. Porównano ekstrakty: wodny (W), etanolowy (ET), glikolowo-wodn...

The study presents changes in the phenolic levels, antioxidant and anti-inflammatory potential of purple basil leaves caused by different chemical elicitors: arachidonic acid (AA), jasmonic acid (JA) and β-aminobutyric acid (BABA). The application of the all tested elicitors increased the concentration of phenolic compounds, including flavonoids an...

This study presents the effect of heat treatment of chickpea seeds on biological activity of peptides obtained by in vitro gastrointestinal digestion. The most significant antiradical activity against ABTS+• expressed as IC50 value was observed for 3.5- to 7-kDa peptide fraction from TC hydrolysate (41.01 μg mL−1). In turn, peptide fraction of 3.5–...

This paper investigates changes in the anti-inflammatory and antioxidative activity of anthocyanins from purple basil (Ocimum basilicum L.) leaves induced by arachidonic acid (AA), jasmonic acid (JA) and β-aminobutyric acid (BABA). The anthocyanins content was significantly increased by all elicitors used in this study; however, no increase was obs...

The aim of the presented study was to characterize the content and biological activity of extracts prepared from dried Stevia rebaudiana leaves with potential application in the food or cosmetic industry. Aqueous (A), ethanolic (E) and glycol-aqueous (GA) extracts were analyzed for the content of polyphenols and proteins, showing that the highest a...

Background. Adzuki sprouts are one of more valuable but still underappreciated dietary supplements which may be considered as functional food. Sprouting reduces anti-nutritional factors and increases the bioavailability of macro and micronutrients and also affects phytochemical levels. Exposure of plants to abiotic stresses results in change in pro...

In this study, a new application of pumpkin pulp in bread production is shown. The aim of this work is to determine the influence of the addition of fresh pumpkin pulp directly into wheat flour on physical, sensorial and biological properties of bread. The bioaccessibility of active compounds was also studied. An increase in the addition of pumpk...

Nowadays, legume plants have been considered not only a source of valuable proteins necessary for the proper functioning and growth of the body but also a source of bioactive compounds such as bioactive peptides, that may be benefi cial to human health and protect against negative change in food. The aim of this study was to investigate the effect...

Pea seeds were fermented by Lactobacillus plantarum 299v in monoculture under different time and temperature conditions and the fermented products were digested in vitro under gastrointestinal conditions. After fermentation and digestion ACE inhibitory activity was determined. In all samples after fermentation no ACE inhibitory activity was noted....

Oatmeal and oat products are an important source of fat, dietary fibre, carbohydrates, mineral compounds, vitamins, and, first of all, proteins. Enzymatic hydrolysis of proteins leads to the release of peptides, which may show various activities including antioxidant activity. The objective of the research study performed was to determine the antio...

MONIKA KARAŚ, ANNA JAKUBCZYK, EDYTA PACZOS-GRZĘDA  WŁAŚCIWOŚCI PRZECIWUTLENIAJĄCE HYDROLIZATÓW BIAŁEK Z ZIARNA UPRAWNYCH I DZIKICH GATUNKÓW OWSA (AVENA L.) S t r e s z c z e n i e Owies i produkty owsiane są ważnym źródłem tłuszczów, błonnika pokarmowego, sacharydów, składników mineralnych, witamin oraz białka. Hydroliza enzymatyczna białek prowad...

Purple basil (Ocimum basilicum L. cv. Dark Opal), cultivated in grown chamber, pretreated with abiotic ehcitors: arachidonic acid (AA), jasmonic acid (JA) and β-aminobutyric acid (BABA). The content of: total phenolics, flavonoids, phenolic acids and anthocyanins in methanolic extracts of basil leaves were determined. Antioxidant activity of obtain...

Proteins of leguminous plants being a source of bioactive peptides characterized by antioxidant and blood pressure lowering properties, can contribute to the prevention of the development of many civilization diseases by inhibiting free radical reactions and delaying the process of cell aging. In this study antioxidative and ACE (angiotensin I-conv...

Recent years have witnessed growing interest in research on the structure and properties of proteins and peptides as physiologically active dietary components. The above has spurred a new interest in the isolation of animal, plant and microbiological peptides and investigation of their biological activity. The isolation and separation of protein an...

Recent research into the structure and properties of proteins and peptides as physiologically active diet components has spurred a new interest in the isolation and investigation of bioactive peptides of animal, plant and microbiological origin. The isolation and separation of protein and peptide mixtures requires advanced procedures. It usually in...

Pea sprouts are a promising source for the production of bioactive compounds that can be beneficial to human health. In this study the peptides were obtained from trypsin hydrolysate from pea sprouts proteins with antiradical and antihypertensive activity ABTS + was used to measure the antiradical capacities, and ACE (angiotensin I-convertmg enzyme...

In this study, peptides and proteins extracted from string bean with 1% TCA were separated by chromatography on columns with copper ions immobilized through IDA and o-phosphoserine OPS. Protein and peptide concentrations and the anti-free-radical properties of the isolated fractions were determined. Identification was obtained using mass spectromet...

The antioxidative properties and chlorophyll contents of protein concentrates obtained by various methods from lucerne were compared. Chloroplast concentrates of lucerne juice (var. Kleszczewska) were precipitated by centrifugation (12 000 g), heating (55°C), or with flocculants (Magnafloc LT-26 and Superfloc A-115). The highest antioxidative activ...