Monika Harnisz

University of Warmia and Mazury in Olsztyn · Department of Environmental Microbiology

The aim of the present study was to analyze changes in the microbiome, resistome, and mobilome of hospital wastewater (HWW) induced by disinfection with chlorine compounds. Changes in bacterial communities and specific antibiotic resistance genes (ARGs) in HWW were determined with the use of a nanopore long-read metagenomic approach. The main hosts...

Antimicrobials and antibiotic resistance genes (ARGs) in substrates processed during anaerobic digestion in agricultural biogas plants (BPs) can reach the digestate (D), which is used as fertilizer. Antimicrobials and ARGs can be transferred to agricultural land, which increases their concentrations in the environment. The concentrations of 13 anti...

Hospitals are regarded as ecological niches of antibiotic-resistant bacteria (ARB). ARB can spread outside the hospital environment via hospital wastewater (HWW). Therefore, HWW is often disinfected in local stations to minimize that risk. Chlorine-based treatment is the most popular method of HWW disinfection around the world, however, recent rese...

Manure fertilization is the primary source of veterinary antimicrobials in the water-soil system. The research gap is the fate of antimicrobials after their release into the environment. This study aimed to provide a detailed and multi-faceted examination of fertilized cultivated fields using two types of manure (poultry and bovine) enriched with s...

Over the past few decades, due to the excessive consumption of drugs in human and veterinary medicine, the antimicrobial resistance (AR) of microorganisms has risen considerably across the world, and this trend is predicted to intensify. Many worrying research results indicate the occurrence of pools of AR, both directly related to human activity a...

Escherichia coli bacteria are an essential indicator in evaluations of environmental pollution, which is why they must be correctly identified. This study aimed to determine the applicability of various methods for identifying E. coli strains in environmental samples. Bacterial strains preliminary selected on mFc and Chromocult media as E. coli wer...

Antibiotics are widely used in human and veterinary medicine, and they are accumulated in various types of waste, including sewage sludge (SS) and cattle slurry (CS), processed by anaerobic digestion (AD). Anaerobic treatment is a method enabling the stabilization of these substrates before transferring to the environment. The presence of contamina...

The aim of this study was to quantify ESKAPEE bacteria, genes encoding resistance to antibiotics targeting this group of pathogens, as well as integrase genes in municipal wastewater and river water. Environmental DNA was extracted from the collected samples and used in deep sequencing with the Illumina TruSeq kit. The abundance of bacterial genera...

Manure is a major source of soil and plant contamination with veterinary drugs residues. The aim of this study was to evaluate the uptake of 14 veterinary pharmaceuticals by parsley from soil fertilized with manure. Pharmaceutical content was determined in roots and leaves. Liquid chromatography coupled with tandem mass spectrometry was used for ta...

Jedną z konsekwencji nadużywania antybiotyków w sektorze zdrowia i rolnictwa oraz zanieczyszczenia środowiska antybiotykami jest wzrost częstości występowania bakterii przenoszących oporność na antybiotyki (ARBs) i genów oporności na antybiotyki (ARGs), które są określane jako nowe zanieczyszczenia środowiska. Uważa się, że wody powierzchniowe, na...

The aim of this study was to develop an effective selective/differential medium for culturing environmental strains of the Bacteroides fragilis group (BFG). This goal was achieved by modifying standard commercial Bacteroides Bile Esculin Agar (BBE Agar). Bacteroides Bile Esculin Agar was combined with substances that inhibit the growth of non-BFG b...

This study aimed to assess the possibility of using solar light-driven photolysis and TiO2-based photocatalysis to remove (1) antibiotic residues, (2) their transformation products (TPs), (3) antibiotic resistance determinants, and (4) genes identifying the indicator bacteria in a treated wastewater (secondary effluent). 16 antimicrobials belonging...

The conversion of cattle manure into biogas in anaerobic digestion (AD) processes has been gaining attention in recent years. However, antibiotic consumption continues to increase worldwide, which is why antimicrobial concentrations can be expected to rise in cattle manure and in digestate. This study examined the long-term synergistic effects of a...

The aim of this study was to evaluate the influence of simultaneous, long-term exposure to increasing concentrations of three classes of antimicrobials (β-lactams, fluoroquinolones and nitroimidazoles) on: (1) the efficiency of anaerobic digestion of sewage sludge, (2) qualitative and quantitative changes in microbial consortia that participate in...

During mechanical-biological treatment, wastewater droplets reach the air with bioaerosols and pose a health threat to wastewater treatment plant (WWTP) employees and nearby residents. Microbiological pollutants and antimicrobial resistance determinants are discharged to water bodies with treated wastewater (TWW), which poses a potential global epi...

The aim of this study was to analyze the prevalence of carbapenem resistance genes in Acinetobacter spp. isolated from wastewater in a municipal WWTP and to determine their spread from treated wastewater to river water with the use of conventional and molecular microbiology methods (qualitative and quantitative PCR and metagenomic analysis). Sample...

The present study was focused on the identification of multi-resistant bacteria from the WHO priority pathogens list in the samples taken from different stages of the full-scale municipal wastewater treatment plant and receiving water. Additionally, the seasonal variations of the selected multi-resistant pathogens were analyzed in the samples. In o...

In this study, the impact of bovine and poultry manure on the quantitative and qualitative composition of antibiotic resistance genes (ARGs) and the environmental mobilome associated with antimicrobial resistance in soil and crops was determined with the use of next generation sequencing methods. The aim of the study was to perform a metagenomic an...

The aim of the work was to develop a new HPLC-MS/MS method that allows for the simultaneous detection of antimicrobials agents (targeted analysis) and their transformation products (non-targeted analysis), which enabled the elucidation of their transformation pathways in the environment. Targeted analysis was performed for 16 selected antimicrobial...

Antibiotic resistance has been considered a major human health threat that may endanger the success of medicine. Recent studies have unveiled worldwide asymmetries of antibiotic resistance occurrence, being factors as diverse as climate, socioeconomic, or antibiotic use possible drivers of such asymmetric distribution. In Europe, where clinical ant...

The diversity of beta-lactam antibiotic resistance genes, with particular emphasis on carbapenemase genes, during the treatment process at two wastewater treatment plants (WWTPs) with different levels of hospital wastewater inflow was investigated using high-throughput sequencing. An additional aspect of the study was to determine the taxonomic div...

Wastewater treatment plants (WWTPs) release drug-resistant microorganisms to water bodies (with effluents), and WWTP employees are exposed to bioaerosol emissions from the processed wastewater. Bacteria of the genus Klebsiella, in particular carbapenemase-producing (CP), hyper-virulent (Hvr) strains of Klebsiella pneumoniae, play a special role in...

The spread of antibiotic resistance is closely related with selective pressure in the environment. Wastewater from industrialized regions is characterized by higher concentrations of these pollutants than sewage from less industrialized areas. The aim of this study was to compare the concentrations of contaminants such as antibiotics and heavy meta...

Conventional mechanical and biological wastewater treatment is unable to completely eliminate all pollutants, which can therefore enter surface water bodies together with treated wastewater. In addition, bioaerosols produced during wastewater treatment can pose a threat to the health of the wastewater treatment plant staff. In order to control the...

This study determined the impact of poultry and bovine manure fertilization on the content of antibiotics, heavy metals (HMs), and the quantitative and qualitative composition of integrase and antibiotic resistance genes in soil, groundwater, and crops cultivated on manure-amended plots. Antibiotic concentration levels were analyzed using the HPLC-...

Due to limited description of the role and diversity of archaea in WWTPs, the aim of the study was to analyze microbial community structures and diversities with particular regard to Archaea in the samples taken from different stages of the full-scale municipal wastewater treatment plant and effluent receiving water (upstream and downstream dischar...

This study aimed to perform a qualitative and a quantitative assessment of the prevalence of genes encoding resistance to beta-lactam, tetracycline, and chloramphenicol antibiotics in samples of DNA isolated from air in a municipal wastewater treatment plant (WWTP) and a municipal waste management plant (WMP). Air samples were collected in the mech...

Aerobic methanotrophs are crucial in ombrotrophic peatlands, driving the methane and nitrogen cycles. Peat mining adversely affects the methanotrophs, but activity and community composition/abundances may recover after restoration. Considering that the methanotrophic activity and growth are significantly stimulated in the presence of other microorg...

The study was designed to simultaneously evaluate the influence of high doses (512-1024 µg/g) the most commonly prescribed antimicrobials on the efficiency of anaerobic digestion of sewage sludge, qualitative and quantitative changes in microbial consortia responsible for the fermentation process, the presence of methanogenic microorganisms, and th...

In line with sustainable development principles and in order to combat climate change, which contributes to progressive soil depletion, various solutions are being sought to use treated sewage sludge as a soil amendment to improve soil quality and enrich arable soils with adequate amounts of biogenic compounds. This review article focuses on the ef...

This study explored the effect of eight antimicrobials on the efficiency of biogas production in the anaerobic digestion (AD) process of cattle manure. The microbiome involved in AD, presence and number of genes mcrA, MSC and MST specific for Archaea, and antibiotic resistance genes (ARGs) concentration in digestate (D) were examined. Supplementati...

This study showed the effect of amoxicillin (AMO), and oxytetracycline (OXY) at a concentration of 512 µg mL−1, and sulfamethoxazole (SMX), and metronidazole (MET) at a concentration of 1024 µg mL−1 on the efficiency of anaerobic digestion (AD) of sewage sludge (SS) and cattle slurry (CS). The production of biogas and methane (CH4) content, and the...

The aim of this study was to evaluate the applicability of markers specific to Bacteroides fragilis group (BFG) bacteria as indicators of anthropogenic pollution of surface waters. In addition, the impact of wastewater treatment plants (WWTPs) on the spread of genes specific to fecal indicator bacteria and genes encoding antimicrobial resistance in...

This study identified differences in the prevalence of antibiotic resistance genes (ARGs) between wastewater treatment plants (WWTPs) processing different proportions of hospital and municipal wastewater as well as various types of industrial wastewater. The influence of treated effluents discharged from WWTPs on the receiving water bodies (rivers)...

Drug-resistant and pathogenic Staphylococcus spp. strains can reach surface waters and air with wastewater evacuated to the environment. These strains increase the environmental pool of genetic determinants conferring antibiotic resistance and virulence, and constitute a health risk for the employees of wastewater treatment plants (WWTP) who come i...

There is a growing concern about the fate of antibiotic resistance genes (ARGs) during wastewater treatment and their potential impacts on the receiving water bodies. We hypothesised that the quantity of ARGs in effluents may be related to the size of wastewater treatment plants (WWTPs) and sampling season. To date, only several attempts have been...

The presence of antibiotic residues, their bioactive metabolites and other transformation products in the environment may adversely affect the organisms that live in the environment and may also contribute to increasing the antibiotic resistance of bacteria. It is particularly difficult to determine the types of contaminants in solid samples, in pa...

In this study, a screening of 26 selected antimicrobials using liquid chromatography coupled to a tandem mass spectrometry method in two Polish wastewater treatment plants and their receiving surface waters was provided. The highest average concentrations of metronidazole (7400 ng/L), ciprofloxacin (4300 ng/L), vancomycin (3200 ng/L), and sulfameth...

Anaerobic digestion (AD) is an organic matter conversion technology which offers a wide range of options for production of biogas from organic biomass, providing an excellent opportunity to convert abundant bioresources into safe, eco‐friendly, renewable energy. Important factors in the process of AD are the biodiversity of microorganisms, chemical...

The aim of this study was to determine the effect of the activated sludge process on the abundance of anaerobic bacteria of the phylum Bacteroidetes, with special emphasis on Bacteroides fragilis group (BFG) bacteria, in twelve full-scale wastewater treatment plants. The composition of bacterial phyla and classes in wastewater samples were analyzed...

Methane fermentation is an attractive practice in waste processing, which enables one to both control pollution and recover energy. This kind of anaerobic digestion is exposed to inhibitors, which can retard the process and cause failure. The mechanism causing toxicity of these substances and their impact on the efficiency of the process are alread...

Antibiotic resistance poses a significant health risk for humans and animals. Wastewater treatment plants (WWTPs) are among the key sources of antibiotic resistance genes (ARGs) in the natural environment. The aim of this study was to analyze the occurrence and seasonal variations of ARGs in treated wastewater flowing to the Pilica River or its tri...

The aim of study was to analyze the occurrence of antibiotic-resistance genes (ARGs) in the Pilica River. Seasonal variations in the occurrence of ARGs in river water were also determined. Water samples were collected in 7 sites situated along a 186-km section of the Pilica River from Przedbórz to estuary. River water was sampled four times between...

Antimicrobial compounds are widely used in human and veterinary medicine to protect human and animal health, to prevent economic losses, and to help to ensure a safe food supply. After administration, the antibiotics and their transformant products (TPs) may pass through the sewage system and end up in the environment, mainly in the water bodies. A...

This book reviews the latest research and developments concerning the biodiversity and biocontamination assessment of aquatic ecosystems in Poland. The authors present expert analyses of the current biological status of Polish surface waters, and examine a broad range of elements related to aquatic ecosystems, including macrophytes, phytoplankton,...

Methane fermentation has been widely used to dispose sewage sludge at wastewater treatment plants (WWTPs), due to production of renewable energy in form of biogas. Antibiotics present in wastewater might accumulate in a sewage sludge. The aim of the present study was to investigate the impact of three antibiotics from different classes in three dif...

The environmental occurrence of antimicrobial pharmaceuticals and antibiotic resistant bacteria and antibiotic resistant genes has become a global phenomenon and a multifaceted threat. Integrated actions of many parties are needed to prevent further aggravation of the problem. Well-directed actions require clear understanding of the problem, which...

Antibiotic resistance is a widespread problem that poses one of the greatest risks to public health around the world. The main cause of antibiotic resistance is the overuse of antibiotics in the human and veterinary medicine and in agriculture. Drugs are released into the environment with treated wastewater, and they can act as stressors that incre...

The uncontrolled use of antibiotics and the release of drug residuals into the environment contribute to antibiotic resistance and constitute a serious public health threat. The spread of antibiotic resistance can be attributed mainly to the huge amounts of bacteria harboring the antibiotic resistance genes (ARGs) which are released into the enviro...

Wastewater treatment plants (WWTPs) are a very important link in the spread of antibiotic resistance genes to the environment and the formation of antibiotic-resistant microorganisms. The mechanical and biological methods of wastewater treatment in WWTPs do not completely remove the resistance genes from sewage. The genes responsible for extended-s...

Wastewater treatment plants (WWTPs) are significant source of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs), which can spread further in the environment by reaching rivers together with effluents discharged from WWTPs. In this study untreated and treated wastewater (UWW, TWW), upstream and downstream river water (URW, D...

Pharmaceutical residues, mainly antibiotics, have been called “emerging contaminants” in the environment because of their increasing frequency of detection in aquatic and terrestrial systems and their sublethal ecological effects. Most of them are undiscovered. Both human and veterinary pharmaceuticals, including antibiotics, are introduced into th...

Anaerobic bacteria of the genus Bacteroides are a large group of commensal microorganisms that colonize the human and animal digestive tract. The genus Bacteroides and the closely related genus Parabacteroides include the Bacteroides fragilis group (BFG) of potentially pathogenic bacteria which are frequently isolated from patients with anaerobic i...

Wastewater treatment plants (WWTPs) are an important reservoir in the development of drug resistance phenomenon and they provide a potential route of antibiotic resistance gene (ARGs) dissemination in the environment. The aim of this study was to assess the role of WWTPs in the spread of ARGs. Untreated and treated wastewater samples that were coll...

The aim of this study was to analyze the effectiveness of identification of Bacteroides fragilis group (BFG) strains isolated from human and rat feces, hospital wastewater, and untreated and treated sewage from a wastewater treatment plant (WWTP). BFG strains were plated on Bacteroides Bile Esculin (BBE) agar. Characteristic colonies were isolated...

Wastewater treatment plants (WWTPs) are major reservoirs of antibiotic-resistant bacteria (ARB) which are transported to the natural environment with discharged effluents. Samples of untreated wastewater (UWW) and treated wastewater (TWW) from four municipal WWTPs and samples of river water collected upstream (URW) and downstream (DRW) from the eff...

Wastewater treatment plants (WWTPs) and municipal waste management plants (MWMPs) emit bioaerosols containing potentially pathogenic biological components which post a threat for human health. Microbiological monitoring supports evaluations of the antibiotic resistance (AR) of airborne microorganisms and the relevant health risks. The aim of this s...

Annually, a few thousand tons of antibiotics and their transformation products (metabolites and degradation products) are introduced to wastewater treatment plants (WWTPs) as a result of human and animal excretion, or dispose of expired or unused medications. Antibiotics present in wastes might inhibit their treatment processes for instance during...

Biological treatment processes at wastewater treatment plants (WWTPs), which are the most common methods of sewage treatment, could cause selective elimination and/or changes in the proportions of phenotypes/genotypes within bacterial populations in effluent. Therefore, WWTPs based on activated sludge used in sewage treatment constitute an importan...

Wastewater treatment plants (WWTPs) are one of the main transmission sources of pathogenic bacteria and antibiotic-resistance genes in the natural environment. In this study, the presence of specific markers of Bacteroides fragilis group (BFG), B. dorei and genes encoding resistance to beta-lactams (cepA, cfxA), tetracyclines (tet(Q)), macrolides,...

Treated wastewater evacuated into the aquatic environment is a potential reservoir of pathogenic and virulent bacteria. The aim of this study was to analyze the presence of genes encoding virulence in E. coli bacteria in samples of untreated (UWW) and treate