Monika Chodasiewicz (Kosmacz)

Monika Chodasiewicz (Kosmacz)
King Abdullah University of Science and Technology | KAUST · Center for Desert Agriculture​

Doctor of Philosophy

About

36
Publications
10,686
Reads
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1,452
Citations
Citations since 2017
29 Research Items
1109 Citations
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Introduction
Currently my research topic focuses on identification of stress specific components (proteins, metabolites and mRNA) of stress granules (SGs) formed under different environmental conditions. My group also aims to understand what are the stress specific components of desert plants, what make them resistant to stressful environmental conditions. This research line involves studying small molecule-protein complexes with biochemical methods combined with molecular and cell biology methods.
Additional affiliations
November 2020 - present
King Abdullah University of Science and Technology
Position
  • Professor (Assistant)
Description
  • At KAUST I am leading a group working on stress granules as well as on identification of stress specific small molecule- protein complexes.
June 2015 - November 2020
Max Planck Institute of Molecular Plant Physiology
Position
  • PostDoc Position

Publications

Publications (36)
Article
Stress Granules (SGs) and Processing-bodies (P-bodies) are biomolecular condensates formed in the cell with the highly conserved purpose of maintaining balance between storage, translation, and degradation of mRNA. This balance is particularly important when cells are exposed to different environmental conditions and adjustments have to be made in...
Article
Full-text available
High temperature is one of the abiotic stresses that plants face and acts as a major constraint on crop production and food security. Plants have evolved several mechanisms to overcome challenging environments and respond to internal and external stimuli. One significant mechanism is the formation of biomolecular condensates driven by liquid–liquid...
Chapter
Protein–metabolite interactions (PMIs) are fundamental for several biological processes. Even though PMI studies have increased in recent years, our knowledge is still limited. The screening of PMIs using small molecules as bait will broaden our ability to uncover novel PMIs, setting the basis for establishing their biological relevance. Here, we d...
Article
Full-text available
The field of stress granules (SGs) has recently emerged in the study of the plant stress response, yet these structures, their dynamics and importance remain poorly characterized. There is currently a gap in our understanding of the physiological function of SGs during stress. Since there are only a few studies addressing SGs in planta, which are p...
Preprint
Full-text available
The role of cyclic adenosine monophosphate (3’,5’-cAMP) in plants is not well understood, and here, we report a novel role of 3’,5’-cAMP in regulating the actin cytoskeleton. The 3’,5’-cAMP treatment affects the thermal stability of 51 proteins, including a vegetative actin isoform, ACTIN2. Consistent with the above results, the increase in 3’,5’-c...
Article
Full-text available
The role of the RNA degradation product 2’,3’-cyclic adenosine monophosphate (2’,3’-cAMP) is poorly understood. Recent studies have identified 2’,3’-cAMP in plant material and determined its role in stress signaling. The level of 2’,3’-cAMP increases upon wounding, in the dark, and under heat, and 2’,3’-cAMP binding to an RNA-binding protein, Rbp47...
Article
Full-text available
Improving yield, nutritional value and tolerance to abiotic stress are major targets of current breeding and biotechnological approaches that aim at increasing crop production and ensuring food security. Metabolic engineering of carotenoids, the precursor of vitamin-A and plant hormones that regulate plant growth and response to adverse growth cond...
Article
Stress granules (SGs) are dynamic membrane-less condensates transiently assembled through liquid–liquid phase separation (LLPS) in response to stress. SGs display a biphasic architecture constituted of core and shell phases. The core is a conserved SG fraction fundamental for its assembly and consists primarily of proteins with intrinsically disord...
Preprint
Full-text available
For many years, 2′,3′-cyclic adenosine monophosphate (2′,3′-cAMP), a positional isomer of the second messenger 3′,5′-cAMP, has not received enough attention. Recent studies have reported that 2′,3′-cAMP exists in plants and might be involved in stress signaling because 1) its level increases upon wounding and 2) it was shown to participate in stres...
Article
Full-text available
How organisms integrate metabolism with the external environment is a central question in biology. Here, we describe a novel regulatory small molecule, a proteogenic dipeptide Tyr-Asp, which improves plant tolerance to oxidative stress by directly interfering with glucose metabolism. Specifically, Tyr-Asp inhibits the activity of a key glycolytic e...
Preprint
Metabolite-protein interactions affect and shape diverse cellular processes. Yet, despite advances, approaches for identifying metabolite-protein interactions at a genome-wide scale are lacking. Here we present an approach termed SLIMP that predicts metabolite-protein interactions using supervised machine learning on features engineered from metabo...
Preprint
Full-text available
Improving yield, nutritional value and tolerance to abiotic stress are major targets of current breeding and biotechnological approaches that aim at increasing crop production and ensuring food security. Metabolic engineering of carotenoids, the precursor of Vitamin-A and plant hormones that regulate plant growth and response to adverse growth cond...
Article
Full-text available
Protein-metabolite interactions are of crucial importance for all cellular processes but remain understudied. Here, we applied a biochemical approach named PROMIS, to address the complexity of the protein-small molecule interactome in the model yeast Saccharomyces cerevisiae. By doing so, we provide a unique dataset, which can be queried for intera...
Article
Full-text available
Recently, we published a set of tobacco lines expressing the Daucus carota (carrot) DcLCYB1 gene with accelerated development, increased carotenoid content, photosynthetic efficiency, and yield. Because of this development, DcLCYB1 expression might be of general interest in crop species as a strategy to accelerate development and increase biomass p...
Article
Full-text available
Plants exhibit different physiological and molecular responses to adverse changes in their environment. One such molecular response is the sequestration of proteins, RNAs, and metabolites into cytoplasmic bodies called stress granules (cSGs). Here we report that, in addition to cSGs, heat stress also induces the formation of SG-like foci (cGs) in t...
Article
Full-text available
Stress granules (SGs) are ubiquitous nonmembrane-bound assemblies of protein and mRNA formed under stress conditions associated with stalled translation. SGs are evolutionarily conserved across eukaryotes. The canonical function of SGs is to selectively protect mRNAs and proteins from unfolding and prevent degradation induced by diverse environment...
Article
Full-text available
Stress granules (SGs) are evolutionary conserved aggregates of proteins and untranslated mRNAs formed in response to stress. Despite their importance for stress adaptation, no complete proteome composition has been reported for plant SGs. Herein, we addressed the existing gap. Importantly, we also provide evidence for metabolite sequestration withi...
Article
Full-text available
Plant response to environmental stimuli involves integration of multiple signals. Upon low-oxygen stress, plants initiate a set of adaptive responses to circumvent an energy crisis. Here, we reveal how these stress responses are induced by combining (i) energy-dependent changes in the composition of the acyl-CoA pool and (ii) the cellular oxygen co...
Article
Full-text available
Small molecules not only represent cellular building blocks and metabolic intermediates, but also regulatory ligands and signaling molecules that interact with proteins. Although these interactions affect cellular metabolism, growth, and development, they have been largely understudied. Herein, we describe a method, dubbed PROtein-Metabolite Intera...
Article
Full-text available
2',3'-cyclic adenosine monophosphate (2',3'-cAMP), an intriguing small molecule, is conserved among different kingdoms. This small molecule is presumably produced during RNA degradation, with increased cellular levels especially upon stress conditions. We observed its presence in protein complexes isolated from native Arabidopsis lysate, suggesting...
Article
Full-text available
Interactions between metabolites and proteins play an integral role in all cellular functions. Here we describe an affinity purification (AP) approach in combination with LC/MS-based metabolomics and proteomics that allows, to our knowledge for the first time, analysis of protein-metabolite and protein-protein interactions simultaneously in plant s...
Article
Full-text available
Protein small molecule interactions are at the core of cell regulation controlling metabolism and development. We reasoned that due to the lack of system wide approaches only a minority of those regulatory molecules are known. In order to see whether or not this assumption is true we developed an effective approach for the identification of small m...
Article
Full-text available
Plants often experience low oxygen conditions as the consequence of reduced oxygen availability in their environment or due to a high activity of respiratory metabolism. Recently, an oxygen sensing pathway was described in Arabidopsis thaliana which involves the migration of an ERF transcription factor (RAP2.12) from the plasma membrane to the nucl...
Chapter
In aerobic organisms oxygen is a rate-limiting substrate for the efficient production of energy, and therefore they need to adjust their metabolism to the availability of oxygen. For this reason, eukaryotes and prokaryotes independently developed mechanisms to perceive oxygen availability and integrate this into developmental and growth programs. D...
Article
Full-text available
Transcriptional activation in response to hypoxia in plants is orchestrated by ethylene-responsive factor group VII (ERF-VII) transcription factors, which are stable during hypoxia but destabilized during normoxia through their targeting to the N-end rule pathway of selective proteolysis. Whereas the conditionally expressed ERF-VII genes enable eff...
Article
Full-text available
In plant and animal cells, amino-terminal cysteine oxidation controls selective proteolysis via an oxygen-dependent branch of the N-end rule pathway. It remains unknown how the N-terminal cysteine is specifically oxidized. Here we identify plant cysteine oxidase (PCO) enzymes that oxidize the penultimate cysteine of ERF-VII transcription factors by...
Article
Full-text available
The crucial role of carbohydrate in plant growth and morphogenesis is widely recognized. In this study we describe the characterization of nana, a dwarf Arabidopsis thaliana mutant impaired in carbohydrate metabolism. We show that the nana dwarf phenotype was accompanied by altered leaf morphology and a delayed flowering time. Our genetic and molec...
Article
Full-text available
The majority of eukaryotic organisms rely on molecular oxygen for respiratory energy production. When the supply of oxygen is compromised, a variety of acclimation responses are activated to reduce the detrimental effects of energy depletion. Various oxygen-sensing mechanisms have been described that are thought to trigger these responses, but they...

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Projects

Projects (4)
Project
Identify proteins and metabolites localized into stress granules. Studding small molecule - protein interactions.
Project
Finding the regulatory function of dipeptides