Mónica Zuzarte

Mónica Zuzarte
University of Coimbra | UC · Institute for Biomedical Imaging and Life Sciences

PhD Biology, University of Coimbra

About

68
Publications
14,525
Reads
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1,583
Citations
Citations since 2016
41 Research Items
1369 Citations
2016201720182019202020212022050100150200250
2016201720182019202020212022050100150200250
2016201720182019202020212022050100150200250
2016201720182019202020212022050100150200250
Additional affiliations
March 2013 - December 2013
Harvard Medical School
Position
  • PostDoc Position
December 2008 - December 2012
University of Coimbra
Position
  • PhD Student
December 2008 - December 2012
University of Coimbra
Position
  • PhD Student

Publications

Publications (68)
Article
Full-text available
Essential oils’ therapeutic potential is highly recognized, with many applications rising due to reported anti-inflammatory, cardioprotective, neuroprotective, anti-aging, and anti-cancer effects. Nevertheless, clinical translation still remains a challenge, mainly due to essential oils’ volatility and low water solubility and stability. The presen...
Article
Full-text available
Arbutus unedo is a Mediterranean shrub or tree that produces edible fruits with promising applications for the food industry and nutraceutical applications. A deep understanding of the cell wall structure and modifications of its components during fruit softening would benefit the development of new food products and retail of fresh fruits. Aiming...
Article
Full-text available
Cardiovascular diseases (CVDs) are the leading cause of mortality worldwide and, together with associated risk factors such as diabetes, hypertension, and dyslipidaemia, greatly impact patients’ quality of life and health care systems. This burden can be alleviated by fomenting lifestyle modifications and/or resorting to pharmacological approaches....
Article
Full-text available
Endoplasmic reticulum (ER) stress and mitochondrial dysfunction, which are key events in the initiation and/or progression of several diseases, are correlated with alterations at ER-mitochondria contact sites, the so-called "Mitochondria-Associated Membranes" (MAMs). These intracellular structures are also implicated in NLRP3 inflammasome activatio...
Article
Full-text available
Exosomes are extracellular vesicles of endosomal origin that are released by practically all cell types across metazoans. Exosomes are active vehicles of intercellular communication and can transfer lipids, RNAs, and proteins between different cells, tissues, or organs. Here, we describe a mechanism whereby proteins containing a KFERQ motif pentape...
Article
For the first time, the present study unravels a cardiospecific therapeutic approach for Pulmonary Arterial Hypertension (PAH), a disease with a very poor prognosis and high mortality rates due to right ventricle dysfunction. We first established a new in vitro model of high-pressure-induced hypertrophy that closely resembles heart defects associat...
Article
Full-text available
Portuguese lavenders remain undervalued in global markets due to the lack of high-quality end-products and scarcity of scientific-based studies validating their bioactive potential. Moreover, chemical variability is frequent in these species, and can compromise both safety and efficacy. In the present study, the anti-inflammatory potential of L. lu...
Article
Full-text available
Lavandula viridis L´Hér. is an endemic Iberian species with a high essential oil yield and a pleasant lemon scent. Despite these interesting features, this species remains unrecognized and poorly explored by the food and pharmaceutical industries. Nevertheless, it has been valued in traditional medicine being used against flu, circulatory problems...
Preprint
Full-text available
Exosomes are extracellular vesicles of endosomal origin released by virtually all cell types across metazoans. Exosomes are active vehicles of intercellular communication and can transfer lipids, RNAs and proteins between different cells, tissues or organs. However, the mechanisms that regulate the selective loading of cytosolic proteins into these...
Chapter
Dermatophytosis is an important health concern with an increasing negative impact due to unsuccessful therapeutic approaches. Natural products have emerged as promising alternative/complementary agents, due to long traditional uses and increasing scientific recognition. Current knowledge on the antidermatophytic activity of natural products, mainly...
Article
Full-text available
Cardiovascular diseases (CVDs) are a global health burden that greatly impact patient quality of life and account for a huge number of deaths worldwide. Despite current therapies, several side effects have been reported that compromise patient adherence; thus, affecting therapeutic benefits. In this context, plant metabolites, namely volatile extra...
Article
Full-text available
Fungal infections remain a major health concern with aromatic plants and their metabolites standing out as promising antifungal agents. The present study aims to assess, for the first time, the antifungal and anti-inflammatory potential of Bupleurum subsp. paniculatum (Brot.) H.Wolff essential oil from Portugal. The oil obtained by hydrodistillatio...
Chapter
Cardiovascular diseases (CVDs) are the leading cause of mortality worldwide with concomitant huge economic and social impacts. These disorders are usually associated with age and several modifiable risk factors such as diabetes, hypertension, and dyslipidemia. Furthermore, oxidative stress and a chronic proinflammatory state, associated with diabet...
Chapter
Cardiovascular diseases (CVDs) remain a leading cause of morbidity and mortality worldwide, accounting for nearly 18 million deaths in 2017. These numbers are expected to escalate in the next decades due to an increase in life expectancy, as well as a higher prevalence of associated risk factors. The burden of CVDs can be alleviated by addressing a...
Article
Full-text available
Ischemic heart disease has been associated with an impairment on intercellular communication mediated by both gap junctions and extracellular vesicles. We have previously shown that connexin 43 (Cx43), the main ventricular gap junction protein, assembles into channels at the extracellular vesicle surface, mediating the release of vesicle content in...
Article
Full-text available
Thymus albicans is an endemic species of the Iberian Peninsula with a vulnerable conservation status. In an attempt to contribute to the valorization of this species, the present study brings new insights on the antifungal and anti-inflammatory mechanism of action of T. albicans essential oil. The antifungal activity of the oil and its major compou...
Article
Full-text available
Deregulation of proteostasis is a main feature of many age-related diseases, often leading to the accumulation of toxic oligomers and insoluble protein aggregates that accumulate intracellularly or in the extracellular space. To understand the mechanisms whereby toxic or otherwise unwanted proteins are secreted to the extracellular space, we inacti...
Article
Ethnopharmacological relevance: Santolina species are widely used in traditional medicine in the Mediterranean region for their anti-inflammatory, antimicrobial, antispasmodic, digestive, and analgesic properties. S. impressa, a Portuguese endemism, is traditionally recognized for its beneficial anti-inflammatory properties in several gastrointest...
Article
Background Cardiovascular diseases (CVDs) are the leading cause of global death with tendency to increase due to population ageing as well as an increase in risk factors for heart disease. Despite improving patient’s survival rate, current therapies are associated with several side effects, directing research towards the development of novel preven...
Article
Full-text available
Fungal infections remain a burden worldwide, thus underpinning the need for effective new therapeutic approaches. In the present study, the antifungal effect of the essential oils of two thyme species, Thymus camphoratus and Thymus carnosus, used in traditional medicine in Portugal, as well as their major compounds was assessed. A special focus was...
Article
Full-text available
Myocardial ischaemia is associated with an exacerbated inflammatory response, as well as with a deregulation of intercellular communication systems. Macrophages have been implicated in the maintenance of heart homeostasis and in the progression and resolution of the ischaemic injury. Nevertheless, the mechanisms underlying the crosstalk between car...
Article
Ethnopharmacological relevance: Thymus camphoratus and T. carnosus are widely used in Portugal for the treatment of inflammatory-related conditions, such as inflammation of the respiratory tract, being the later also used as an antitussive. Aim of the study: Bearing in mind the lack of scientific studies focused on the pharmacological activity o...
Data
List of the 513 nuclear genes related to mitochondrial function and structure sequenced by Next Generation Sequencing (Mitome500 panel, Unpublished, Lee-Jun C. Wong).
Data
List of the sequence variations detected in nuclear genes in patient's samples, after filtering for variant calling.
Data
Glycolytic function measured for patient and controls' cells. Data are representative of the mean ± SEM. (A) Acidification profile; (B) Glycolysis; (C) Glycolytic Capacity; (D) Glycolytic Reserve.
Data
Assessment of enzymatic activity of the mitochondrial respiratory chain complexes. Results are expressed as nmol of substrate per min per mg of protein normalized to Citrate Synthase. Patient values are presented as the mean of replicates. Control values are shown as mean ± SD.
Data
Mitochondrial sequence variations detected by whole mitochondrial genome sequencing in the patient presenting CPEO.
Data
Representation of mt-tRNASer(UCN) steady-state levels by high-resolution northern blot in skeletal muscle, without significant differences between patient and controls.
Article
Full-text available
Chronic Progressive External Ophthalmoplegia (CPEO) is characterized by ptosis and ophthalmoplegia and is usually caused by mitochondrial DNA (mtDNA) deletions or mt-tRNA mutations. The aim of the present work was to clarify the genetic defect in a patient presenting with CPEO and elucidate the underlying pathogenic mechanism. This 62-year-old fema...
Article
Full-text available
Aims: MicroRNAs (miRNAs) have been implicated in the pathogenesis of pulmonary hypertension (PH), a multifactorial and progressive condition associated with an increased afterload of the right ventricle leading to heart failure and death. The main aim of this study was to correlate the levels of miR-424(322) with the severity and prognosis of PH a...
Article
Aims: Myocardial infarction (MI) is the leading cause of morbidity and mortality worldwide and results from an obstruction in the blood supply to a region of the heart. In an attempt to replenish oxygen and nutrients to the deprived area, affected cells release signals to promote the development of new vessels and confer protection against MI. How...
Article
Full-text available
Here we report the rational design of a new third-generation photosensitizer (PS), a chlorin conjugated with galactodendritic units, ChlGal8, to improve the effectiveness of bladder cancer treatment. ChlGal8 shows better photo-chemical and -physical properties than a recently reported homologous porphyrin, PorGal8. In addition to inheriting excelle...
Data
Presence of Cx43 in extracellular vesicles reduces the cardiotoxicity of the anti-tumour therapeutic approach with doxorubicin
Article
Full-text available
Background: Cardiovascular diseases (CVDs) are the leading cause of morbidity and mortality worldwide that seriously affect patient's life quality and are responsible for huge economic and social burdens. It is widely accepted that a plant-based diet may reduce the risk of CVDs by attenuating several risk factors and/or modulating disease's onset...
Article
Full-text available
Rosmarinic acid (RA) possesses several protective bioactivities that have attracted increasing interest by nutraceutical/pharmaceutical industries. Considering the reduced bioavailability after oral use, effective (and safe) delivery systems are crucial to protect RA from gastrointestinal degradation. This study aims to characterize the safety prof...
Article
Full-text available
The essential oil of Daucus carota subsp. carota from Portugal, with high amounts of geranyl acetate (29.0%), α -pinene (27.2%), and 11 α H-himachal-4-en-1 β -ol (9.2%), was assessed for its biological potential. The antimicrobial activity was evaluated against several Gram-positive and Gram-negative bacteria, yeasts, dermatophytes, and Aspergillus...
Article
Full-text available
The importance of Alternaria sp. fungi to human health ranges from being etiological agents of serious infections with poor prognosis in immunosuppressed individuals to their association with respiratory allergic diseases. The present work focuses on Alternaria infectoria, as a model organism of the genus, and was designed to unravel melanin produc...
Article
Daucus carota (carrot) essential oil is one of the most valued by-product of this species due to its medicinal properties. The present study focuses on the antifungal and anti-inflammatory activity of the unexplored essential oil of wild carrot (D. carota subsp. maximus) from Portugal. The essential oil from the aerial parts of plants was analysed...
Chapter
Essential oils are complex mixtures of volatile compounds produced by aromatic plants and extracted by distillation or expression. More than 3000 essential oils are known with about 300 being commercially relevant as perfume, cosmetic, cleaning, food, and pharmaceutical products. The main trade oils (over 1000 t/year) are those extracted from Citru...
Article
Full-text available
Intercellular communication is vital to ensure tissue and organism homeostasis and can occur directly, between neighbour cells via gap junctions (GJ), or indirectly, at longer distances, through extracellular vesicles, including exosomes. Exosomes, as intercellular carriers of messenger molecules, mediate the transfer of biological information betw...
Poster
O medronheiro (Arbutus unedo L.) é uma espécie originária da orla do Mediterrâneo e da Irlanda. Os caules e folhas jovens encontram-se cobertos por numerosos tricomas que secretam uma substância mucilaginosa. Estes tricomas e o seu secretado nunca foram caracterizados e a sua função é desconhecida. Com o objectivo de caracterizar em termos morfológ...
Article
The present study was conducted to elucidate the composition, antifungal and anti-inflammatory activities and cytotoxicity of Daucus carota subsp. gummifer essential oil. Aerial parts of the plants were hydrodistilled and the oil analyzed by GC and GC-MS. The oil was characterized by high contents of monoterpenes (83.9%), the major compounds being...
Article
Full-text available
Lavandula multifida (fernleaf lavender) grows in confined regions of Portugal. This species is known for its interesting chemical profile, rich in phenolic compounds, and significant antifungal and anti-inflammatory properties, making it an interesting plant for industrial exploitation and justifying alternative propagation methods. Therefore, the...
Article
Full-text available
Gap junction intercellular communication (GJIC) between cardiomyocytes is essential for synchronous heart contraction and relies on Connexin (Cx)-containing channels. Increased breakdown of Cx43 has been often associated with various cardiac diseases. However, the mechanisms whereby Cx43 is degraded in ischemic heart remain unknown. The results obt...
Article
As part of our ongoing study on the valorization of aromatic plants, the present study was designed to elucidate the composition, scavenging potential, anti-inflammatory activity and cytotoxicity of Margotia gummifera essential oils.Umbels were submitted to hydrodistillation in a Clevenger-type apparatus and the oils were analyzed by GC and GC–MS....
Article
Lavandula L. and Thymus L. comprise several relevant species for the food, cosmetic, perfumery and pharmaceutical industries. Considering the traditional medicinal use of L. stoechas and T. herba-barona and the lack of scientific studies on their biological activities, the present study was designed to elucidate the composition and antifungal activ...
Article
This work reports the antifungal activity of Lavandula luisieri essential oils against yeast, dermatophyte and Aspergillus strains responsible for human infections and food contamination. The oil's cytotoxicity and its effect on the yeast-mycelium transition in Candida albicans, an important virulence factor, were also evaluated. Analyses by GC and...
Article
As part of our ongoing work on the valorization of Portuguese lavenders we explored both the anti-inflammatory potential of Lavandula viridis essential oil (EO) and its mechanism of action. The EO was isolated by hydrodistillation and analysed by GC and GC/MS using fused silica capillary columns with two different stationary phases. The oil was ch...