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Mónica Fernández Lorenzo de Mele

Mónica Fernández Lorenzo de Mele
National University of La Plata, CONICET · INIFTA

Professor

About

130
Publications
7,013
Reads
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3,293
Citations
Citations since 2016
28 Research Items
1500 Citations
2016201720182019202020212022050100150200
2016201720182019202020212022050100150200
2016201720182019202020212022050100150200
2016201720182019202020212022050100150200
Introduction
Biomaterials Nanomaterials and Nanostructures Bioadsorbable materials Biofilms Natural antimicrobials
Additional affiliations
April 1978 - present
National University of La Plata AND CONICET
Position
  • Professor (Full), Senior Researcher

Publications

Publications (130)
Article
The development of new natural phenolic-enable nanotechnologies with multifunctional purposes is related to the exceptional physicochemical properties conferred by the phenolic moieties. Phenolic phytocompounds can oxidize and self-polymerize to assemble on surfaces and form diverse coatings. They interact either in non-covalent or covalent form wi...
Article
Full-text available
The use of metal-based and, particularly, Al2O3 nanoparticles (Al2O3-NP) for diverse purposes is exponentially growing. However, the growth of such promissory market is not accompanied by a parallel extensive investigation related to the impact of this pollution on groundwater and biological systems. Pseudomonas species, ubiquitous, environmentally...
Preprint
Full-text available
The use of metal-based and, particularly, Al 2 O 3 nanoparticles (Al 2 O 3− NP) for diverse purposes is exponentially growing. However, the growth of such promissory market is not accompanied by a parallel extensive investigation related to the impact of this pollution on groundwater and biological systems. Pseudomonas species, ubiquitous, environm...
Article
Full-text available
The effects of environmental factors such as sunlight irradiation and the presence of humic acid (HA) on the physicochemical properties of commercial multiwall carbon nanotubes (MWCNT) suspended in a simulated inorganic matrix (SIM) and their impacts on bacteria growing in biofilms were evaluated. Both solar irradiation and the presence of HA lead...
Chapter
Essential oils (EOS) or volatile oils are secondary metabolites produced by aromatic plants. They are obtained from diverse parts of these plants (leaves, seeds, berries, flowers, wood, peels, etc.) by different extraction processes. Their uses date back to ancient cultures who bequeathed to us their knowledge about EOs benefits. Several studies ha...
Chapter
Several biodegradable metals (Cu, Mg and Fe and its alloys) are used when temporary implants are needed or for copper-based intrauterine devices (IUD). However, their degradation rates are usually not suitable for the implant site requirements or for an adequate healing process and, consequently, strategies to control their degradation rates are re...
Article
The degradation of bioresorbable metals in vivo changes the physicochemical properties in the environment of an implant, such as a stent in the artery wall, and may induce the alteration of the functions of the surrounding cells. The Fe-degradation, from bioresorbable stents, is a particularly intricate process because it leads to the release of so...
Article
Immobilization of PEG-covered Silicon Dots, PEGSiDs, on glass substrates was performed following a simple strategy involving particle embedding by a sol-gel process forming a silica film on glass slides. The obtained films, denoted as fSiOx-PEGSiD, constitute a water-wettable, strongly supported, photoluminescent glass coating. The films showed hig...
Article
Abstract Objective To develop an antimicrobial and anti-adherent thymol (TOH)-containing coating on titanium (Ti) by a bioinspired one-step biocompatible method. Methods A nanolayer of adsorbed TOH (TOH-NL-Ti) was formed by an easy deep coating method on Ti surface. The treatment consists in a simple one-step immersion process in a TOH-containing...
Article
Full-text available
Carvacrol (Carv) and thymol (TOH), components of essential oils, are known by their antimicrobial and antioxidant activity. However, Carv but not TOH seems to be the responsible of anti-inflammatory and inhibition of Cu corrosion properties. Since Carv and TOH are positional isomers, their identification is tricky and GC-MS is usually required. To...
Article
Full-text available
The control of multidrug-resistant (MDR) bacteria is a growing public health problem, and new strategies are urgently needed for the control of the infections caused by these microorganisms. Notoriously, some MDR microorganisms generate complex structures or biofilms, which adhere to surfaces and confer extraordinary resistance properties that are...
Article
The present study is focused on the development of a novel surface by electrochemical modification of titanium substrates (Ti) to enhance bone-bonding ability. A Ce-containing conversion coating was formed on Titanium oxide nanotubes (Ti_TiO2NT) (Ti_TiO2NT_CeOx) in an innovative and straightforward approach by anodic deposition. Both Ti_TiO2NT and...
Article
Copper bearing IUDs main disadvantage is the initial high release (burst release) of copper ions that causes an increased inflammatory response in surrounding tissues and several side effects. In this work, we studied the performance of Cu coated with an electropolymer obtained by potentiostatic polarization of a phytocompound, carvacrol, at 400 mV...
Article
The prevention of microbial biofilm formation on a biomaterial surface is crucial in avoiding implants failures and the development of antibiotic resistant bacteria. It was reported that biodegradable Mg alloys may show antimicrobial effects due to the alkalinization of the corroding area. However, this issue is controversial and deserves a detaile...
Article
Trans-resveratrol (3, 5, 4′ trihydroxystilbene, RSV) is a natural compound that shows antioxidant, cardioprotective, anti-inflammatory and anticancer properties. The transdermal, painless application of RSV is an attractive option to other administration routes owing to its several advantages like avoiding gastrointestinal problems and first pass m...
Article
Medical device-related infections represent a major healthcare complication, resulting in potential risks for the patient. Antimicrobial materials comprise an attractive strategy against bacterial colonization and biofilm proliferation. However, in most cases these materials are only bacteriostatic or bactericidal, and consequently they must be use...
Article
Reactive oxygen species (ROS) are an efficient tool to eradicate microorganisms, due to the capacity of these species to damage almost all types of biomolecules and to kill cells. The increment in the resistance mechanisms to antibiotics leads to the exploration of new strategies to eliminate microorganisms that involves production of ROS, such as...
Article
Full-text available
The interactions that could be built between the biomaterials and tissue- microenvironments are very complex, especially in case of degradable metals that generate a broad variety of degradation products. The interfacial problems are particularly relevant for Fe-based materials that have been proposed for the development of biodegradable implants....
Article
With the intention of taking care of the environment and human health, the development of alternative eco-friendly methods to inhibit metal corrosion is intensively encouraged. In previous works we showed that some phytocompounds components of essential oils such as carvacrol (Carv) and thymol (TOH) are able to be electropolymerized on metals and t...
Chapter
Intrauterine device (IUD) is the second most widely used contraception method. The historical background of this device and the advantages and disadvantages of this method are analysed in this chapter. Different aspects were taken into account. Among them, the need of a detailed study of the release of copper during the first days is discussed in r...
Article
Grafting of polyethylene glycol (PEG) to ultrasmall photoluminescent silicon dots (SiDs) is expected to improve and expand the applications of these particles to aqueous environments and biological systems. Herein we report a novel one-pot synthesis of robust, highly water compatible PEG-coated SiDs (denoted as PEG-SiDs) of (3.3 ± 0.5) nm size. The...
Article
A comparative study of the electropolymerization of two natural phenolic isomers, carvacrol (Carv) and thymol (TOH) on copper was made. They are components of Origanum vulgare and Thymus vulgaris essential oils. Polymerized layers (polyCarv and polyTOH) were attained by cyclic voltammetry (CV). Electrochemical techniques complemented by ATR-FTIR, X...
Article
Biofilms formed on implanted devices are difficult to eradicate. Adhesion mechanism, high bacterial density, aggregation, induction of persisters and stressed bacteria are some of the factors considered when the antimicrobial resistance of these biofilms is analyzed. The aim of this work was to provide an alternative approach to the understanding o...
Article
The release of copper ions by copper-containing devices, equipments and facilities represents a potential risk for biological systems. Different inhibitory treatments (CuIT) that use organic compounds have been proposed to reduce this environmental hazard but many of them are not in accordance with new regulations. The development of an ecofriendly...
Article
Mg-based implants have promising applications as biodegradable materials in medicine for orthopedic, dental, and cardiovascular therapies. During wear and degradation microdebris are released. Time-lapse multidimensional microscopy (MM) is proposed here as a suitable tool to follow, in fixed intervals over 24-h periods, the interaction between cell...
Article
This work is focused on the processes occurring at the bioabsorbable metallic biomaterial/cell interfaces that may lead to toxicity. A critical analysis of the results obtained when degradable metal disks (pure Mg and rare earth-containing alloys (ZEK100 alloys)) are in direct contact with cell culture and those obtained with indirect methods such...
Article
Microbial related contamination is of major concern and can cause substantial economic losses. Photodynamic inactivation (PDI) has emerged as a suitable approach to inhibit microorganism proliferation. In this work, PDI induced by 6-carboxypterin (Cap), a biocompatible photosensitizer (PS), was analyzed. The growth inhibition of Staphylococcus aure...
Article
Rare earth (RE) elements have been proposed to improve the corrosion resistance of degradable Mg alloys for medical applications. However, good biocompatibility of the elements released by Mg alloys during degradation is essential for their use in implants. Most studies are focused on material science and engineering aspects, but the effects of ion...
Chapter
Full-text available
Some specific clinical problems, particularly those related to orthopedic trauma and some cardiovascular diseases need only temporary support for healing. This support can be provided by biodegradable metallic materials such as, Fe-, Mg- based alloys that avoid some of the side effects of traditional biomaterials. They are expected to support the h...
Article
The use of copper-based alloys for fixed dental crowns and bridges is increasingly widespread in several countries. The aim of this work is to study the dissolution of a zinc-aluminum-bronze and the cytotoxic effects of the ions released on UMR-106 osteoblastic cell line. Two sources of ions were used: (1) ions released by the metal alloy immersed...
Article
Full-text available
Local resolved electrochemical measurement setup, MCS, will present for demonstration of the power for a non-destructive assessment of metallic biomaterial interfaces under living near conditions. As an example the experiments on orthodontic wires are presented.
Article
The copper intrauterine device (IUD) based its contraceptive action on the release of cupric ions from a copper wire. Immediately after the insertion a burst release of copper ions occurs, which may be associated to a variety of side effects. 6-mercaptopurine (6-MP) and pterin (PT) have been proposed as corrosion inhibitors to reduce this harmful r...
Article
The detrimental effects of biofilms are a cause of great concern in medical, industrial and environmental areas. In this study, we proposed a novel eradication strategy consisting of the combined use of micropatterned surfaces and antibiotics on biofilms to reduce the rate of bacterial colonisation. Pseudomonas fluorescens biofilms were used to per...
Article
The formation of biofilms on titanium dental implants is one of the main causes of failure of these devices. Streptococci are considered early colonizers that alter local environment favouring growing conditions for other colonizers. Chlorhexidine (CHX) is so far the most effective antimicrobial treatment against a wide variety of Gram-positive and...
Article
Mg is a biodegradable biomaterial which may release particles (MP) to the environment. The possible cyto- and genotoxic effects of MP derived from magnesium powder (mesh 325) were analyzed on rat osteosarcoma UMR106 cells in order simulate the effect of Mg debris. Neutral red (NR) incorporation and acridine orange/ethidium bromide (AO/EB) staining...
Article
Mg-based materials are promising for orthopedic, dental, and cardiovascular applications but their high degradation rate in vivo (release of Mg ions and debris particles) is cause of great concern. Protective treatments involving fluoride conversion coatings have been proposed in order to reduce corrosion rates. The aim of this study was to evaluat...
Article
The copper intrauterine device is a contraceptive method that is based on the release of copper ions from a copper wire. Immediately after insertion, the dissolution of copper in the uterine fluid is markedly higher ("burst release") than that necessary for contraception action, leading to a variety of harmful effects. Pretreatments with organic co...
Article
The development of a biodegradable metallic implant demands a precisely defined degradation profile and adequate mechanical properties. Mg has been proposed for this purpose but it has an excessively high corrosion rate and insufficient yield strength. In the present work pure Mg mechanically reinforced by a powder metallurgy (Mg(PM)) route and tre...
Article
Biofilm development involves several stages and flagellar expression of bacteria is considered an important factor in this process. However, its role in the earliest stage of biofilm development is not yet clear. In order to analyse this topic, Pseudomonas fluorescens samples were trapped on a patterned gold surface with sub-microtrenches (ST) so a...
Article
Previous studies have shown that pure Mg that is reinforced by powder metallurgy (Mg(PM)) is a promising biodegradable material, because it circumvents the drawbacks of the toxic components of Mg alloy implants. However, its degradation rate is high, which can be reduced with fluoride coatings (Mg(PM)F). This protective effect is temporary, because...
Article
Titanium is a corrosion-resistant and biocompatible material widely used in medical and dental implants. Titanium surfaces, however, are prone to bacterial colonization that could lead to infection, inflammation, and finally to implant failure. Silver nanoparticles (AgNPs) have demonstrated an excellent performance as biocides, and thus their integ...
Article
This paper describes bacterial organization on nano/micropatterned surfaces with different chemical properties, which show different interactions with the biological systems (inert, biocompatible, and bactericide). These surfaces were prepared by molding techniques and exposed to Pseudomonas fluorescens (P. fluorescens) cultures. Results from atomi...
Article
The aim of this study was to analyze the cytotoxicity and genotoxicity of titanium oxide (TiO(2)) and aluminium oxide (Al(2)O(3)) nanoparticles (NPs) on Chinese hamster ovary (CHO-K1) cells using neutral red (NR), mitochondrial activity (by MTT assay), sister chromatid exchange (SCE), micronucleus (MN) formation, and cell cycle kinetics techniques....
Article
The aim of this work was to collect and compare data from different published reports which focused on the description of the influence of different electrochemical setups for the assessment of magnesium corrosion. Based on this, a comparison with our own results, obtained for LAE 442 and AZ 31, was made and discussed. As the collection of data has...
Chapter
Full-text available
Biofilms are communities of microorganisms consisting in a biologically active aggregate of cells immersed in extracellular polymeric substances (EPS) and attached on a live or dead surface. Atomic force microscopy (AFM) and optical microscopy (OM), including epifluorescence microscopy (EM) have been proved to be suitable tools in order to follow t...
Article
Pure Mg has been proposed as a potential degradable biomaterial to avoid both the disadvantages of non-degradable internal fixation implants and the use of alloying elements that may be toxic. However, it shows excessively high corrosion rate and insufficient yield strength. The effects of reinforcing Mg by a powder metallurgy (PM) route and the ap...
Article
Magnesium and its alloys are highly degradable metals that are potentially useful as biomaterials, especially in orthopaedic and cardiovascular applications. However, the in vivo corrosion has proved to be too high. Because of the complexity of in vivo conditions, a careful study of the corrosion of magnesium in synthetic solutions that simulate th...
Article
The corrosion behaviour of AZ31 magnesium alloy with different grain sizes immersed in simulated body fluids was compared in chloride solution (8 gl(-1)) and in phosphate-buffer solution (PBS). The influence of immersion time was also analyzed. Electrochemical techniques such as open circuit potential, polarization curves, transient currents and el...
Article
Copper wire is the main component of a type of intrauterine device used as a contraceptive. Its contraceptive effect is attributed to the copper ions released as a result of the dissolution of copper in the uterus. Even though 10-year intrauterine device life is estimated on the basis of the dissolution rate of copper measured in vivo and in vitro,...
Article
This work focuses on the possible use of phenanthro[9,10-c]-1,2,5-thiadiazole 1,1-dioxide (TDZ) as a harmless corrosion inhibitor. TDZ range-dose providing minimum adverse effects to the environment and human health, with satisfactory corrosion-inhibiting properties was evaluated. Cytotoxicity and genotoxicity of TDZ at 0.57-12.50 microM concentrat...
Article
Bacterial adhesion and spreading on biomaterials are considered key features of pathogenicity. Roughness and topography of the substrate have been reported to affect bacterial adhesion, but little is known about their effect on spreading. Submicron row and channel tuning with bacterial diameter (S2) were designed to test bacterial motility on these...
Article
The cytotoxicity potential of TiO(2) and Al(2)O(3) nanoparticles (NP) in UMR 106 cells was studied by evaluating the lysosomal activity with neutral red uptake assay (NR), and the mitochondrial activity with tetrazolium MTT test. Different NP concentrations (10-300 microg/mL range) were used. A significant (p < 0.001) increase in the absorbance (st...
Article
Application of a biomaterial requires profound knowledge about the features and structure of the biomaterial - tissue interface. Electrochemical techniques, especially electrochemical impedance spectroscopy (EIS) enable a fast non-destructive characterization of the interface. This review summarizes the application of EIS in different biological en...
Article
The aim of this study was to investigate the cytotoxic and genotoxic effect of copper extracts obtained from metallic copper in Chinese hamster ovary (CHO-K1) cell line using neutral red (NR), sister chromatid exchange (SCE), chromosomal aberrations (CA) and cell-cycle kinetics tests. Cells were cultured in Ham-F10 with different copper-containing...
Article
Most of the implantable medical devices are prone to infection caused by microorganisms that form biofilms. Pseudomonads are frequently used as model species for studying bacterial adhesion. The initial stages of biofilm formation are influenced by different factors including, among others, the chemistry of the surface, the roughness, and topograph...
Article
The extended use of metallic biomaterials yields to increasing sources of metal ions within the human body and may result in inflammation of the surrounding tissues, cell damage, and cancer. The aim of this study was to investigate the relationship between the radial diffusion of metal ions released from a metal disk by the corrosion process and th...
Article
The adhesion of Pseudomonas fluorescens was studied on nano/microengineered surfaces. Results show that these bacteria formed well-defined aggregates on randomly oriented nanosized granular gold substrates. These aggregates consist of aligned ensembles of bacteria, with some of them strongly elongated. This kind of biological structure was not foun...
Article
Full-text available
Biomaterials failures are frequently associated to the formation of bacterial biofilms on the surface. The aim of this work is to study the adhesion of non motile bacteria streptococci consortium and motile Pseudomonas fluorescens. Substrates with micro and nanopatterned topography were used. The influence of surface characteristics on bacterial ad...
Article
Full-text available
Magnesium is potentially useful for orthopaedic and cardiovascular applications. However, the corrosion rate of this metal is so high that its degradation occurs before the end of the healing process. In industrial media the behaviour of several magnesium alloys have been probed to be better than magnesium performance. However, the information rela...
Article
Biomaterials failures are frequently associated to the formation of bacterial biofilms on the surface. The aim of this work is to study the adhesion of non motile bacteria streptococci consortium and motile Pseudomonas fluorescens. Substrates with micro and nanopatterned topography were used. The influence of surface characteristics on bacterial ad...
Article
The aim of this paper was to evaluate the possible enhancement of the biocidal efficacy of glutaraldehyde against Pseudomonas fluorescens biofilms by the application of an electric field. The behaviour of sessile cells and cells released by the biofilms was assed. Biofilms were formed on thin stainless steel coupons immersed in culture media inocul...
Article
Ions released from metallic dental materials used in orthodontic appliances could induce undesirable effects on cells and tissues. This study evaluates the biocompatibility of two of the most labile components of metallic dental alloys on osteoblastlike cells. The influence of protein and ions on metal dissolution properties is also investigated us...
Article
Ions released from metallic dental materials used in orthodontic appliances could induce undesirable effects on cells and tissues. This study evaluates the biocompatibility of two of the most labile components of metallic dental alloys on osteoblastlike cells. The influence of protein and ions on metal dissolution properties is also investigated us...
Article
The aim of this work is to contribute to the elucidation of the cytotoxic process caused by the copper ions released from the biomaterials. Clonal cell lines UMR106 were used in the experiments. Copper ions were obtained from two different sources: copper salts and metal dissolution. Experiments carried out with constant ion concentrations (copper...
Article
Microbiological control in industrial environments is frequently restricted to monitor the number of planktonic (suspended) cells. However, this number is not often related with the microorganisms attached to the surfaces (sessile bacteria). The aim of this research work was to develop a method for monitoring biofilm growth and the biocidal efficac...
Article
The combination of a conventional optical microscope with a specially designed glass flow cell was used to visualize in situ biofilms formed on opaque thin biomaterials through a simple non-invasive way (optical microscopy of thin biofilms, OMTB). Comparisons of OMTB with scanning electron microscopy (SEM) images were made. Thin metallic dental bio...