Monchai Duangjinda

• Ph.D.
• Head of Faculty at Khon Kaen University

Hemp (Cannabis sativa L.) leaf oil (HLO) contains several bioactive compounds such as phenolics, flavonoids, and quercetin. However, the effects of HLO on hypertensive conditions have not yet been investigated. This study investigated the cardiovascular protective effects of HLO in a nitric oxide (NO) synthase inhibitor-induced hypertensive rat mod...

Days open (DO) is a critical economic and reproductive trait that is commonly employed in genetic selection. Making improvements using conventional genetic techniques is exceedingly challenging. Therefore, new techniques are required to improve the accuracy of genetic selection using genomic data. This study examined the genetic approaches of tradi...

Heat stress severely affects dairy cattle production and reproduction performances in tropical regions. Genetic selection to maintain adequate yield and reproductive performance while enhancing their ability to withstand heat is essential for improving the genetics of dairy cows. Therefore, in this study, we aimed to estimate genetic parameters aff...

Thai native chickens hold significant value in rural communities due to their adaptability and suitability for local rearing conditions. However, there are challenges in the commercial raising of these chickens. This study aimed to evaluate and promote the potential of Thai native chickens (Pradu Hang Dum) for commercial purposes and the developmen...

Reproductive traits are important traits that directly affect a farmer’s income and are difficult to improve upon using traditional genetic methods. Therefore, there is a need to consider new options for increasing the accuracy of the genetic selection of dairy cows. The objective of this study was to compare the genetic methods of the traditional...

Toll-like receptors (TLRs) are transmembrane proteins important for directing immune responses. Their primary role is to recognize pathogens based on single-nucleotide polymorphism (SNP) characteristics. TLR2 is categorized as a pattern recognition receptor (PRR) that is important for the recognition of pathogens. Nucleotide variation in the coding...

Objective: To investigate polymorphisms of the MC4R and IGF2 genes and to evaluate the growth traits affected by such polymorphisms in Thai native (Kradon) pigs. Methods: Blood samples and productive data from 91 Kradon pigs were collected. DNA was extracted and quantified, the IGF2 and MC4R genes were amplified, and the polymerase chain reactio...

Skin hyperpigmentation is an aesthetic problem that leads to psychosocial issues. Thus, skin whitening agents from agro- and poultry-industrial co-products are considered high economic value ingredients of interest for sustainable application. Therefore, this study aimed to determine the cosmeceutical potential of anserine/carnosine-rich chicken ex...

This study aimed to evaluate the effect of a high protein diet comprising breast meat from commercial broiler (BR), Thai native (PD), and commercial broiler × Thai native crossbred (KKU-ONE) chicken on serum uric acid, biochemical parameters, and antioxidant activities in rats. Male Sprague–Dawley rats were divided into four groups. The control gro...

The purpose of this study was to determine the combining abilities and heterosis for the growth performance and carcass characteristics in crosses between Hmong black-bone (HB), Chinese black-bone (CB), and Thai native (TN) chickens using a mating system diallel crossing. Nine crossbred chickens including HB × HB, CB × CB, TN × TN, HB × TN, TN × HB...

Thailand is a tropical country affected by global climate change and has high temperatures and humidity that cause heat stress in livestock. A temperature–humidity index (THI) is required to assess and evaluate heat stress levels in livestock. One of the livestock types in Thailand experiencing heat stress due to extreme climate change is crossbred...

Heat stress is becoming a significant problem in dairy farming, especially in tropical countries, making accurate genetic selection for heat tolerance a priority. This study investigated the effect of heat stress manifestation on genetics for milk yield, milk quality, and dairy health traits with and without genomic information using single-step ge...

Halal products are growing in consumer markets worldwide, and pork meat is classified as non‐halal. Manufacturers of processed foods and products must ensure that their products follow Islamic dietary law because pork is prohibited for Muslims. Droplet digital polymerase chain reaction (PCR) (ddPCR) is a novel method for identifying pig species and...

Heat stress is becoming a major problem because it limits growth in poultry production, especially in tropical areas. The development of genetic lines of Thai native chickens (TNC) which can tolerate the tropical climate with the least compromise on growth performance is therefore necessary. This research aims to analyze the appropriate growth curv...

Cow genotypes are expected to improve the accuracy of genomic estimated breeding values (GEBV) for young bulls in relatively small populations such as Thai Holstein-Friesian crossbred dairy cattle in Thailand. The objective of this study was to investigate the effect of cow genotypes on the predictive ability and individual accuracies of GEBV for y...

The objectives of this study were to investigate changes in genetic parameters for milk yield (MY) and heat tolerance of the crossbred Thai Holstein Friesian population under different heat stress levels over time, and to investigate the threshold point of heat stress manifestation on milk production. Genetic parameters were estimated using single-...

This study investigated the effects of low-cost dried yeast and multi-strain probiotics on laying hens. A total of 108 Charoen Pokphand (CP) brown hens aged 25 weeks were used in a completely randomised design (CRD) experiment involving 18 cages of six hens per replicate and six replicates per group. The hens were allocated to three groups: basal d...

This study aimed to investigate the carnosine content and ATP-grasp domain-containing protein 1 (CARNS1) gene expression and their relationship with breast meat of Black Chinese (BC), KU-Phuparn (KP), Pradu Hang Dam (PD), and Black Chinese × Pradu Hang Dam (Sri Mok: SM) to aid in the selection and mating programs for developing functional meat in T...

Abstract Background Extreme panting under heat stress threatens dairy cattle milk production. Previous research has revealed that the gas exchange-mediated respiratory drive in critically ill dairy cattle with low O2 saturation induces panting. Vascular endothelial growth factor (VEGF) signaling may play important roles in immunosuppression and oxi...

The objective of this study was to estimate genetic parameters for age at first farrowing (AFF), length of productive life (LPL), lifetime number of piglets born alive (LNBA), lifetime number of piglets weaned (LNW), lifetime wean to first service interval (LW2S), and lifetime pig efficiency (LTP365) from commercial Thai Landrace and Large White po...

Objective: The objectives of this study were to investigate the relationship between the mRNA expression of Adipocyte type Fatty Acid Binding Protein (A-FABP) and Heart type FABP (H-FABP) in Thai native chicken crossbreeds and evaluate the level of exotic inclusion in native chicken that will improve growth while maintaining its relatively low car...

This study identified anserine and anserine/carnosine in chicken breast of Thai native chicken (TNC; 100% Thai native), Thai synthetic chicken (TSC; 50% Thai native), and Thai native crossbred chicken (TNC crossbred; 25% Thai native) compared with commercial broiler chicken (BR; 0% Thai native) using nuclear magnetic resonance (NMR) spectroscopy an...

The objective of this study was to compare the appropriate models used to estimate the value of genetic parameters in fertility traits: fertility (FER), hatchability of fertile eggs (HOF), and hatchability of eggs set (HOS) in Thai native (Pradu Hang Dam) chickens. Data were collected for each fertility trait from 3435 test-week records from 715 he...

Background: Extreme panting under heat stress threatens dairy cattle milk production. Previous research has revealed that the gas exchange-mediated respiratory drive in critically ill dairy cattle with low O2 saturation induces panting. Vascular endothelial growth factor (VEGF) signaling may play important roles in immunosuppression and oxidative s...

This study aimed to study the role of PPARs on fat deposition in native crossbred chicken. We studied the growth, abdominal, subcutaneous, and intramuscular fat, and mRNA expression of PPARA and PPARG in adipose and muscle tissues of four chicken breeds (CH breed (100% Thai native chicken), KM1 (50% CH background), KM2 (25% CH background), and broi...

Background: Crossbreeding using exotic breeds is usually employed to improve the growth characteristics of indigenous chickens. This mating not only provides growth but affect adversely to fat deposition as well. We studied the growth, abdominal, subcutaneous and intramuscular fat and mRNA expression of peroxisome proliferator-activated receptor (P...

Objective: The objective of the study was to investigate the possibility of utilizing an early litter size trait as an indirect selection trait for longevity and to estimate genetic parameters between sow stayability and litter size at different parities using a linear-threshold model for longevity in Thai Large White and Landrace populations. Me...

The aims of this study were 1) estimate heritability and variance components for sow survival traits using random regression model and 2) to identify the best model when conducting a sow survival genetic evaluation for Thailand commercial farms by comparing RRM with different covariance functions (Legendre polynomial and linear spline). A total of...

Objective: The objective of this study was to estimate the genetic parameters and trends for milk, fat, and protein yields in the first three lactations of Thai dairy cattle using a 3-trait,-3-lactation random regression test-day model. Methods: Data included 168,996, 63,388 and 27,145 test-day records from the first, second, and third lactation...

V-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT) is a major gene affecting Dominant white and belt-like coat colors in domestic pigs breed. Therefore, the study were designed to investigate the genetic variability of intron 19 of KIT gene (two nucleotide substitutions: g.276G>A and g.295A>C) and to identify breed-specific alleles...

Objective: The purpose of this study was to investigate a single step genome-wide association study (ssGWAS) for identifying genomic regions affecting on reproduction trait in Landrace and Large White pigs. Methods: The traits included the number of pigs weaned per sow per year (PWSY), the number of litters per sow per year (LSY), pigs weaned pe...

The objective of this study was to determine the effect of fermented total mixed rations (FTMR) differing in calculated methane production on in vivo methane emission and performance of lactating dairy cows. Four mid-lactation Holstein-Friesian dairy cows, averaging 424.2±23.0 kg BW and yielding 13.86±7.05 kg/d milk,were used in an experiment based...

The objective of this study was to determine the effect of feeding two fermented total mixed ration (FTMR) on methane production in dairy heifers. Four Holstein Friesian dairy heifers with mean body weight (BW) of 301.1 ± 42.2 kg were used in a simple cross-over design trial. Treatments consisted of 2 FTMR formulated to yield either low (LMD) or hi...

In this study, we estimated the genetic parameters and identified the putative quantitative trait loci (QTL) associated with the length of productive life (LPL), days open (DO), and 305-day milk yield for the first lactation (FM305) of crossbred Holstein dairy cattle. Data comprising 4,739 records collected between 1986 and 2004 were used to estima...

Heat tolerance in poultry production was obtained attention due to the need for genetic lines that can withstand climate changes. This study aimed at investigating heat tolerance in commercial and native broiler genetics, as well as the physiological and growth performance responses of HSP70 genotypes submitted to heat stress. In Experiment I, hete...

The objective of this study was to compare different testing strategies of pigs involving direct measurement of feed efficiency in pig. Using simulated data software, we collected individual information for FCR and grouped trials into direct and indirect measurement. The direct FCR included measurement of FCR in both sexes (100M-100F), in boars onl...

The objective of this research is to investigate appropriate random regression models with various covariance functions, for the genetic evaluation of test-day egg production. Data included 7,884 monthly egg production records from 657 Thai native chickens (Pradu Hang Dam) that were obtained during the first to sixth generation and were born during...

The aims of this study were to estimate, simultaneously, the genetic parameters of test-day milk fat-to-protein ratio (FPR), test-day milk yield (MY), and days-open (DO) in the first two lactations of Thai Holsteins. A total of 76 194 test-day production records collected from 8874 cows with 8674 DO records between 2001 and 2011 from different lact...

The objective of this work was to develop a DNA markers for identification of ten cattle breeds in Thailand: North Eastern Thai Native cattle (ENC) , Northern Thai Native cattle (WL) , Brahman cattle (BRA), Holstein Friesian cattle (HF); Tak cattle (TK), Thai Milking Zebu (TMZ), Kamphaeng Saen cattle (KPS), crossbred Native x Brahman (BN), Pon Yang...

The test-day milk fat-to-protein ratio (TD-FPR) could serve as a measure of energy balance status and might be used as a criterion to improve metabolic stability and fertility through genetic selection. Therefore, genetic parameters for fertility traits, test-day milk yield (TD-MY) and TD-FPR, as well as, their relationships during different stages...

The objective of this research was to investigate the optimum fat to protein ratio (FPR) in Thai tropical Holstein dairy cattle. First parity data consisting of 20 492 milk yields (MY) records for 24 891 cows for the period 2001 and 2011, were used in the analysis. The analysis used a random regression test-day animal model of third-order Legendre...

Animal species identification has received growing attention, regarding genetic diversity and food traceability. The objective of this study is to apply a universal primer of part of the mitochondrial 16S rRNA gene analysis using the PCR-RFLP and HRM methods for identification of species origin in cattle, chicken, horse, sheep, pig, buffalo, and go...

The use of antioxidants as semen additives in the extender has been proposed to improve sperm function during cool storage. The objective of this study was to compare the effects of different antioxidant supplementation in extender on boar semen quality during storage for 10 days at 17 degrees C. Semen samples (n=18) were divided into 10 aliquots a...

This study was designed to: (i) estimate genetic parameters and breeding values for conception rates (CR) using the repeatability threshold model (RP-THM) and random regression threshold models (RR-THM); and (ii) compare covariance functions for modeling the additive genetic (AG) and permanent environmental (PE) effects in the RR-THM. The CR was de...

Cattle commonly raised in Thailand have characteristics of Bos indicus (zebu). We do not know when or how cattle domestication in Thailand occurred, and so questions remain regarding their origins and relationships to other breeds. We obtained genome-wide SNP genotypic data of 28 bovine individuals sampled from four regions: North (Kho-Khaolampoon)...

Mutations in melanocortin 1 receptor (MC1R) gene and v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT) gene have been shown to affect coat color patterns in pigs. Additional functional marker genes, such as insulin like growth factor-2 (IGF2) and orphan nuclear receptor, germ cell nuclear factor (NR6A1), have been described for va...

Molecular marker selection has been an acceptable tool in the acceleration of the genetic response of desired traits to improve production performance in chickens. The crossbreds from commercial parent stock (PS) broilers with four Thai synthetic breeds; Kaen Thong (KT), Khai Mook Esarn (KM), Soi Nin (SN), and Soi Pet (SP) were used to study the as...

The objectives of this study were to compare covariance functions (CF) and estimate the heritability of milk yield from test-day records among exotic (Saanen, Anglo-Nubian, Toggenburg and Alpine) and crossbred goats (Thai native and exotic breed), using a random regression model. A total of 1472 records of test-day milk yield were used, collected f...

The objective of this study was to estimate genetic parameters for various fertility traits on Holstein upgraded dairy heifers and cows in a smallholder system under tropical conditions using data sets from the Thailand national recording scheme. The investigated traits were age at first service (AFS), age at first calving (AFC), days from calving...

Animal species identification has received growing attention, regarding genetic diversity and food traceability. The objective of this study is to apply a universal primer of part of the mitochondrial 16S rRNA gene analysis using the PCR-RFLP and HRM methods for identification of species origin in cattle, chicken, horse, sheep, pig, buffalo, and go...

The supply chain for pigs is a long chain that is not resilient to environmental changes. The most vulnerable point in the chain occurs at the pig production level, which consists of breeding units, great grandparents, grandparents, parents and fattening units. Similar to other agricultural chains, the pig chain is susceptible to disruptions, such...

Indigenous pigs of Thailand have been raised in the remote area of Northern, NorthEastern , Western and Southern regions. The indigenous pig population was rapidly decreasing due to low productive performance. Conservation program should be set up for genetic conservation. The study of diversity of indigenous pigs is necessary for conservation plan...

Heat stress in tropical regions is a major cause that strongly negatively affects to milk production in dairy cattle. Genetic selection for dairy heat tolerance is powerful technique to improve genetic performance. Therefore, the current study aimed to estimate genetic parameters and investigate the threshold point of heat stress for milk yield. Da...

The conservation of genetic variation is essential for meeting future unpredictable breeding requirements. Therefore, the foremost priority must be given to assessment of the population variation status. Among several markers, a DNA based microsatellite is a choice for the population studies, currently. We reviewed some important statistical tools...

Diabetes mellitus (DM) of a first degree relative is an important risk factor for developing type 2 diabetes mellitus (T2DM). The study investigated whether genetic polymorphism of the cytokine interleukine-1β (−511C/T) (IL-1B) was associated with the development of type 2 diabetes mellitus (T2DM) among first degree relative having diabetes mellitu...

Cutaneous evaporation is the main avenue by which cattle dissipate heat via the involvement of sweat glands and other skin components. The difference in skin morphology between B. indicus and B. taurus has been recognized, as well as differences in their ability to tolerate heat. The objective of this study was to compare skin morphology between B....

Four Thai synthetic chicken lines (Kaen Thong, Khai Mook Esarn, Soi Nin, and Soi Pet) originated from Thai native and exotic commercial chickens were evaluated for their growth and carcass traits with the purpose of developing a Thai broiler breeding program. Insulin-like growth factor I (IGF-I) gene is known to play an important role in growth, pr...

Clinical mastitis (CM) is a major health problem causing profit loss in the dairy industry in tropical hot/humid countries. The aim of this study was to detect quantitative trait loci (QTL) influencing CM. The evaluation was carried out in two steps. First, clinical mastitis was measured and the breeding values (EBV) were estimated. Second, animals...

Our study investigated proteins in bovine spermatozoa using 2-DE and LC MS/MS and found a markedly different expression in mature bovine proacrosin binding protein (sp32), which is the focus of the present study. Spermatozoa extract from nine fertile Brahman bulls was separated using 2-DE followed by Coomassie brilliant blue staining. From over 600...

Developing a synthetic breed is one efficient way to establish high growth terminal boar. Pakchong 5 (PC5) is a synthetic breed that is made up of Duroc (DR) and Pakchonge 2 (PC2), which is derived from the inter se mating of the third generation of DR and Pietrain (PT) pigs. The PC5 swine have been selected for five generations on economically imp...

The impact of avian influenza (AI) on Thai indigenous chicken genetic resources following the outbreak of the disease in 2004 forms the main objective of this review. A survey was performed on 482 households from 27 villages in seven sub-districts. A total of 482 households representing the North, Northeast and central regions of Thailand were exte...

Genetic characterization among Red Junglefowl (GS, Gallus gallus spadiceus), Thai indigenous chicken (TIC, Gallus domesticus) and commercial lines has been widely used for studies of genealogical origin, genetic diversity, and effects of selection. We compared the efficiency of genetic characterization of chicken populations that had been under dif...

The objective of this study was to compare models for suitable genetic parameter estimation of birth weight (BWT) and Kleiber ratio (KR) in a population admixture of Thai native, Anglo-Nubian, and Saanen goats. The data set from 51 bucks and 700 does contained 3020 records of BWT and KR. Data were analyzed using five bivariate animal models with di...

The genetic diversity of Bhutanese chickens needs to be understood in order to develop a suitable conservation strategy for these birds in Bhutan. In this, work, we used microsatellite markers to examine the genetic diversity of Bhutanese chickens. Four Bhutanese chicken varieties (Black plumage, Frizzle, Naked neck and Red Junglefowl-like, corresp...

Poor semen quality has long been associated with bull infertility. However, the molecular basis in spermatozoa cells underlying the mechanisms of bull infertility remain unknown. The purpose of this study was to determine whether there is any protein in bovine spermatozoa related to semen quality. Semen samples from 18 Brahman bulls, 3 to 10 yrs of...

This study investigated the effects of varying the Soluble Protein (SP) and sugar concentration in total mixed rations. Four crossbred Thai native steers, 241±26 kg BW, fitted with rumen cannulae were arranged in a 4×4 Latin square design with a 2×2 factorial arrangement of treatments. The steers were fed treatment rations with varied SP (60.0 or 8...

Seasonality of days open (DO) for Thai crossbred Holsteins was examined and genetic effects of heat stress on DO open were determined. Data included 18,413 records for first and second parities of 12,162 cows that calved between 1990 and 2006. Least squares means for DO were estimated using a model with fixed effects of herd-year of calving, breed...

The threshold for heat stress on milk yield of Holstein crossbreds under climatic conditions in Thailand was investigated, and genetic effects of heat stress on milk yield were estimated. Data included 400,738 test-day milk yield records for the first 3 parities from 25,609 Thai crossbred Holsteins between 1990 and 2008. Mean test-day milk yield ra...

A total of 210 chicken samples, from seven strains, were genotyped using 20 microsatellite loci of which 16 are recommended by the Food and Agriculture Organization. The genetic variability and divergence of four Thai indigenous strains and three commercial lines were assessed to generate baseline information for conservation, promotion, and make s...

This research aimed to develop algorithms for calculating some important indices that determine the reproductive and production efficiency of swine breeding herds. Algorithms were developed for litters per mated female per year (LMFY) and pigs weaned per mated female per year (PWMFY). Both algorithms can be used to develop an information system for...

A Web-based Information System (WIS) was developed using open source software (Linux Operating System, Apache web server, MySQL and PHP, which are collectively known as LAMP) to assist swine breeding herd management in data recording and tracing, as well as in problem diagnosis. A farm survey was conducted to obtain basic information including prob...

Bovine leukocyte antigen DRB3 alleles from Holstein x Zebu crossbred dairy cows (n = 409) were analyzed using the PCR-RFLP technique. Exon II of DRB3 was amplified using locus-specific primers (HLO30/HLO32), followed by digestion with 3 restriction enzymes (RsaI, BstyI, and HaeIII). Forty alleles were found with frequency ranging from 0.005 to 0.13...

Genetic parameters were estimated for birth weight and weaning weight in Anglo-Nubian, Saanen, Thai native and crossbred goat populations maintained at the Yala Livestock Research and Breeding Center. Records on 2,845 kids descended from 54 bucks and 615 does from 1995 to 2005 were included in the analysis. Analyses were carried out using the DFREM...

The experiment was carried out during the 2003 to 2006 at the Department of Fisheries, Khon Kaen University, Khon Kaen, Thailand in collaboration with the Department of Biosciences, the University of Hertfordshire, College Land, Hatfield, Herts, UK. Molecular RAPD technique was used for the determinations of DNA patterns of the fish genus Puntius H...

This investigation was carried out at the Department of Fisheries, Khon Kaen University, Khon Kaen, Thailand in collaboration with the Department of Biosciences, University of Hertfordshire, Hatfield, United Kingdom. The work was carried out in the 2003 to 2006 to taxonomically identify fish of the genus Puntius Hamilton 1822 harvested from 15 wetl...