Mona A. Hussein

• Professor at Agricultural & Biological Research Institute-National Research Centre, Egypt

Both hunting billbug, Sphenophorus venatus and white grub, Phyllophaga crinita are considered the most destructive pest for golf courses not only in Egypt but worldwide. This work is considered the first record of P. crinita in Egypt. So, many efforts were carried out to control these pests. In this work nanopesticides formulations were used agains...

Abstract—Normal formulations of some organic chemicals were developed to nano-organic chemicals and tested against the fifth instar larvae of the black cutworm, Agrotis ipsilon. These organic chemicals include etofenprox, imidacloprid and indoxacarb. The nanosizes of these formulations ranged between 246 to 390 nm. The loading capacities were 60.7...

Background: Entomopathogenic nematodes have been used as biopesticides for controlling insect pests in niche markets. Many new isolates have been recovered from soil of natural habitats in many parts of the world. Native EPNs may be more suitable for controlling local pests. Therefore, isolation and identification of native nematode-bacterial assoc...

Four pesticides (thiacloprid, etofenprox, spirotetramat and fenbutatin oxide) were evaluated against the adults of chocolate banded snail, Eobania vermiculata under laboratory conditions. Two types of pesticide formulations were used; conventional formulations (suspension concentrate and emulsifier concentrate) and nanoformulations (nanoparticles)....

Chitinases have many applications in food, agricultural, medical, and pharmaceutical fields. This study succeeded in investigating Aspergillus niger MK981235 chitinase in the spot of its physiochemical, kinetic, thermodynamic, and application. The optimum temperature, pH and p-nitrophenyl-β-d-N-acetyl glucosaminide (PNP-β-GlcNAc) concentration to o...

Background: Entomopathogenic nematodes (EPNs) are obligate lethal parasites of insects and are globally used as safe biocontrol agents against a wide range of insect pests. They occur in most agricultural soils all around the world. The insecticidal activity of two native EPNs Heterorhabditis bacteriophora (BA1) and Steinernema carpocapsae (BA2), i...

Three Gram-stain-negative, rod-shaped, non-spore-forming bacteria, BA1 T , Q614 T and PB68.1 T , isolated from the digestive system of Heterorhabditis entomopathogenic nematodes, were biochemically and molecularly characterized to clarify their taxonomic affiliations. The 16S rRNA gene sequences of these strains suggest that they belong to the Gamm...

Three common pesticides with different pesticide groups were used in this work. These pesticides include; chlorfenapyr (pyrroles group), imidacloprid (neonicotinoids group) and flonicamid (pyridinecarboxamide group). Two types of formulations of these pesticides were used; conventional and nanoformulations against the adults of glossy clover snails...

JBiopest 12(1): 134-138 (2019) EPN to Thrips tabaci © 576 Efficiency of three formulated entomopathogenic nematodes against onion thrips, Thrips tabaci under aquaculture system ABSTRACT Entomopathogenic nematodes (EPN) proved to be efficient control agents against thrips under soil stages. However, few studies were reported on foliar application of...

Combined effect of substerilizing doses of gamma radiation (40 and 100 Gy) and different concentrations of the entomopathogenic nematode, Steinernema carpocapsae BA2 (20 and 40 IJs/ml) on the hemocyte count of the greater wax moth, Galleria mellonella (L.) larvae was studied. Eight types of hemocytes were described in the hemolymph of the normal la...

Entomopathogenic nematodes (EPN) have several important attributes that make them excellent candidates for biological control of soil insects. These nematodes can be produced by in vivo by baiting technique on insects and commercially by in vitro solid/liquid culturing. This study is an attempt for in vitro mass production of two native isolates of...

Molecular identification of parasitic nematode and its pathogenicity against three species of land gastropods

Terrestrial gastropods are economic pests of horticultural crops, vegetables and nurseries in Egypt. Biological control using slug parasitic nematodes is one of the alternatives of chemical control to avoid the hazards of pesticides. This paper describes the finding of a new species of nematode parasite in Limax flavus slug in Egypt. It was identif...

Abstract Background Land snails, especially the chocolate banded snails, Eobania vermiculata are destructive pests of a wide range of field and vegetable crops. New pesticides in different pesticides groups were used against the adults and eggs of chocolate banded snail, E. vermiculata under laboratory conditions. These pesticides include indoxacar...

JBiopest 12(1): 134-138 (2019) EPN to Thrips tabaci © 576 Efficiency of three formulated entomopathogenic nematodes against onion thrips, Thrips tabaci under aquaculture system ABSTRACT Entomopathogenic nematodes (EPN) proved to be efficient control agents against thrips under soil stages. However, few studies were reported on foliar application of...

Background: Land snails, especially the chocolate banded snails, Eobania vermiculata are destructive pests of a wide range of field and vegetable crops. New pesticides in different pesticides groups were used against the adults and eggs of chocolate banded snail, E. vermiculata under laboratory conditions. These pesticides include indoxacarb, abame...

Background:Land snails, especially the chocolate banded snails,Eobania vermiculataare destructive pests of a wide range of field and vegetable crops. New pesticides in different pesticides groups were used against the adults and eggs of chocolate banded snail,E. vermiculataunder laboratory conditions. These pesticides include indoxacarb, abamectin,...

Two novel Schiff base ligands of 2-amino 3- cyano 1, 5 diphenylpyrrole and salicylaldehyde (HL1) or 2- hydroxy11-naphthylaldehyde (HL2) and their copper (II) complexes were synthesized and characterized. Their effect on the infectivity and reproduction potential of the Egyptian entomopathogenic nematodes (EPN’s) Heterorhabditis bacteriophora and th...

Soil samples from different Egyptian Governorates as Giza, Behera, Alexandria, Sohag, Qulupeia, El-Sadat City, Sharkia, Bani-Swif, North Sinai (El-Arish) and South Sinai (Ras-Seder), Tanta, Fayoum and Suez have been surveyed for isolation of new entomopathogenic nematode isolates. One isolate coded as IB was isolated from Sharkia (Belbies). Based o...

The toxicity effects of Dipel 2x, Radiant, Proclaim, Aphox and Coragen insecticides to two species of entotmopathogenic nematodes (Heterorhabditis bacteriophora BA1 and Steinernema carpocapsae BA2), was evaluated under laboratory conditions at different field rates 100%, 50% and 25% field rates (FR). Moreover, the virulence of untreated (control) a...

Sequential optimization strategy was achieved based on statistical experimental designs for enhancement of Aspergillus awamori EM66 exochitinase production. Firstly, 2-level Plackett-Burman design was accomplished for distinguishing between the bioprocess parameters, which improve the exochitinase production. Second optimization step was implemente...

Sequential optimization strategy was achieved based on statistical experimental designs for enhancement of Aspergillus awamori EM66 exochitinase production. Firstly, 2-level Plackett–Burman design was accomplished for distinguishing between the bioprocess parameters, which improve the exochitinase production. Second optimization step was implemente...

Field studies were conducted on diamondback moth (DBM) larvae, Plutella xylostella (Linnaeus) (Lepidoptera: Plutellidae), a foliar pest infesting watercress planted under aquaponic agriculture system. The work was targeted to improve efficiency of two native entomopathogenic nematodes (EPNs), (Steinernema carpocapsae BA2 and Heterorhabditis bacteri...

This study deals with using the products of waste recycle to preserve our environment. Serial concentrations of the rice husk oil were applied in laboratory against the 4th larval instar of Galleria mellonella, the results showed that the larval mortality increased with increasing the concentrations. The samples were collected from treated larvae a...

An assessment of quality traits of two nematode species; Steinernema carpocapsae BA2 and Heterorhabditis bacteriophora HP88 that have been reared for long time on larvae of the greater wax moth Galleria mellonella L. was carried out before and after passing them for one generation through two host species. Alternative hosts were larvae of the leopa...

Field studies were conducted on diamondback moth (DBM) larvae, Plutella xylostella (Linnaeus) (Lepidoptera: Plutellidae), a foliar pest infesting watercress planted under aquaponic agriculture system. The work was targeted to improve efficiency of two native entomopathogenic nematodes (EPNs), (Steinernema carpocapsae BA2 and Heterorhabditis bacteri...

Photorhabdus temperata strain Meg1 is an entomopathogenic bacterium that forms a symbiotic association with Heterorhabditis nematodes. We report here a 4.9-Mbp draft genome sequence for P. temperata strain Meg1, with a G+C content of 43.18% and containing 4,340 candidate protein-coding genes. Copyright © 2014 Hurst et al.

Field surveys were carried out during the period from 2006-2008 at Giza, Behera, Alexandria, Sohag , Qulupeia, El-Sadat city, Sharquia, Bani-swif, North Sinai (El- Aresh city) and South Sinai (Ras-Seder city), Tanta, Fayoum and Suez. Four isolates were isolated from 593 samples. Based on morph metric characters, three strains were found to belong t...

The cotton leaf worm Spodoptera littoralis and the black cut worm, Agrotis ipsilon (Lepidoptera: Noctuidae) are common destructive pests of many economic vegetable and field crops including corn (Zea mays). They attack the corn seedlings causing damage of the inner whorl leaves and cutting the plant stems above the soil surface. Both insects hide i...

Photorhabdus luminescens strain BA1 is an entomopathogenic bacterium that forms a symbiotic association with Heterorhabditis nematodes. We report here a 5.0-Mbp draft genome sequence for P. luminscens strain BA1, with a G+C content of 42.46% and 4,250 candidate protein-coding genes.

The ultrastructure studies on the haemolymph of 5th larval instar of Galleria mellonella showed five types of haemocytes; Prohaemocytes, Plasmatocytes, Granulocytes, Oenocytoids and Spherulocytes. After treatment with Steinernema carpocapsae BA2, the haemocytes underwent considerable structural changes. More destructive effects were observed in the...

Two surveys were conducted at an organic farm, 60 km northeast of Cairo, Egypt, in 2009 and 2011 to study the occurrence, population density and distribution of plant-parasitic nematodes associated with different crops. A total of 216 soil samples were collected from vegetables, fruits and herbs during both surveys. Eleven genera of plant-parasitic...

Four low cost artificial diets for mass rearing the greater wax moth, Galleria mellonella L. were compared to the natural food i.e. honey-bee-wax. The results showed that diet 1: (Wheat flour 350 g, corn flour 200 g, milk powder 130 g, backing yeast powder 70 g, honey 100 ml, and glycerin 150 ml), diet 2: (Wheat flour 350 g, corn flour 200 g, milk...

In many commercial EPNs-based biopesticide companies, formulations ranging from the impregnation of EPNs on artificial sponge to highly advanced granular formulations have been developed. Major challenges have included the development of room-temperature shelf stability, ease of use, and contamination control. The aim of this work was to evaluate t...

Sugar beet, Beta vulgaris L. is a strategic crop of sugar industry in Egypt. It is threatened by several insect pests among most important of them is the beet fly Pegomyia mixta. This work deals with the biological control of this insect using four entomopathogenic nematodes (EPNs). The nematodes included Steinernema carpocapsae S2, Steinernema fel...

Sugar beet, Beta vulgaris L. is a strategic crop of sugar industry in Egypt. It is threatened by several insect pests among most important of them is the beet beetle Cassida vittata. This work deals with the biological control of this insect using four Egyptian and imported entomopathogenic nematodes (EPNs). The nematodes included Steinernema carpo...

Two entomopathogenic nematodes (EPNs) and an egg parasitoid for controlling three corn borers (Sesamia cretica, Chilo agamemnon and Ostrinia nubilalis) in corn fields. EPNs, Heterorhabditis bacteriophora (BA1) and Steinernema carpocapsae (BA2) were applied to the control S. cretica (after 40 d post planting) on different planting dates. Three relea...

The efficiency of entomopathogenic nematodes (EPN) and chemical insecticides against melon fruit flies Dacus ciliatus (Loew) infecting cucurbits in Egypt was evaluated. Five specie of EPN (2 species of Steinernema and 3 of Heterorhabditis) and two insecticides were screened. In general,the data indicated that Steinernema feltiae (NC) and Heterorhab...

Two entomopathogenic nematode strains (Beth 11 and Beth 22) were isolated by trapping with larvae of Galleria mellonella from soil samples taken from the fields near Bethlehem, West Bank. The total length of the nematode dauer juveniles fall within the description for Heterorhabditis indica. Crossbreeding experiments confirmed the designation of th...

Research was conducted to determine whether the snakeweed flower head weevil, Anthonomus tenuis Fall, showed either feeding differences or differential larval survival between broom snakeweed, Gutierrezia sarothrae (Pursh) Shinners, and threadleaf snakeweed, G. microcephala (DC) Gray. Results indicate that while adult weevils feed on both species,...