
Moloya GohainDBT-North East Centre for Agricultural Biotechnology
Moloya Gohain
B.Sc., M.Sc., Ph.D.
About
22
Publications
8,702
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293
Citations
Introduction
Additional affiliations
November 2018 - present
DBT-North East Centre for Agricultural Biotechnology
Position
- Researcher
Description
- 1. Plant Genomics 2. Climate-Smart Agriculture
July 2013 - October 2015
Education
August 2005 - July 2008
Publications
Publications (22)
The intermixing of hereditary material between crossable species occurs invariably in nature. Today, genome engineering tools integrated with plant breeding, allow precise intermixing of genetic traits for the improvement of crop species. However, this requires the identification of candidate gene(s) from genetic resources within or outside the gen...
Maturing oocytes have diverse developmental potential and good quality oocytes exhibit a better ability to attain physiological milestones in a time-dependent manner. This situation necessitates the confirmation of oocyte developmental status more precisely under an in vitro embryo production (IVEP) regime. The aim of this study was to explain time...
Oryza sativa indica (cv. IR64) and Oryza sativa japonica (cv. TNG67) vary in their regeneration efficiency. Such variation may occur in response to cultural environments that induce somaclonal variation. Somaclonal variations may arise from epigenetic factors, such as DNA methylation. We hypothesized that somaclonal variation may be associated with...
The hierarchical organization of chromatin is known to associate with diverse cellular functions; however, the precise mechanisms and the 3D structure remain to be determined. With recent advances in high-throughput next generation sequencing (NGS) techniques, genome-wide profiling of chromatin structures is made possible. Here, we provide a compre...
The copy number variation (CNV) is the number of copies of a particular gene in the genotype of an individual. Recent evidences show that the CNVs can vary in frequency and occurrence between breeds. These variations reportedly allowed different breeds to adapt to different environments. As copy number variations follow Mendelian pattern of inherit...
Abstract Background Tissue culture is used to establish desired phenotypes in plants. Callus culture and regeneration are critical steps in this process. Calli of Oryza sativa japonica (cv. TNG67) and Oryza sativa indica (cv. IR64) have varying regeneration efficiencies. Genetic diversity could partly account for such differences but the epigenetic...
The effect of follicle-stimulating hormone (FSH) on apoptotic status of cumulus cells, expression of proapoptotic and antiapoptotic genes, and development rate of in vitro fertilization–produced buffalo embryos were investigated. FSH supplementation in in vitro maturation–medium resulted in a dose-dependent reduction in the expression of proapoptot...
In eukaryotes, the heat shock factors (HSFs) are recognized as the master regulator of the heat shock response. In this respect, the genes encoding the heat shock factors seem to be important for adaptation to thermal stress in organisms. Despite this, only few mammalian HSFs has been characterized. In this study, four major heat shock factor genes...
Germinal Vesicle Break Down (GVBD) marks the first significant event towards successful completion of
meiosis in oocytes. However, in the chronology of oocyte development, this event must happen in a precise time course so that the oocytes are left with enough opportunity to complete other required changes and eventually attaining full fertilizatio...
The removal of crossbred bulls from semen collection programs due to the production of poor quality semen causes substantial monetary losses to the dairy industry. Seminal quality, a quantitative trait, is greatly influenced by genome level variations. Deletion and/or duplication of Y chromosomal genes and subsequent changes in gene copy number hav...
##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
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Background
The Y chromosome in mammal is paternally inherited and harbors genes related to male fertility and spermatogenesis. The unique intra-chromosomal recombination pattern of Y chromosome and morphological difference of this chromosome between Bos taurus and Bos indicus make it an ideal model for studying structural variation, especially in c...
##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
Global warming due to climate change is likely to aggravate the thermal/heat stress in buffaloes, affecting their reproductive and productive performance. Stress levels during a day have been worked out using Temperature Humidity Index (THI) calculated from dry bulb and wet bulb temperature monitored every ten minutes during 2005-07. The analysis r...
A polymerase chain reaction, amplifying a fragment of the mitochondrial DNA D loop region was developed for species specific detection of cattle and buffalo milk. The method was simultaneously extended for detection of HTST pasteurized milk samples and cheeses of bovine and buffalo origin. A common forward primer was used with two different species...
Questions
Questions (2)
Single cell RRBS and WGBS is reported in both mice and human.
For RNA obtained from a single cell not only the low concentration demands a highly sensitive instrument, but also the volume of total RNA isolated is a limitation. Not more that 1-3uL can be spared for the sake of quantitation. Nanodrop and Qubit did not serve our purpose.