
Mokhtar Ibrahim DabbourBenha University · Department of Agricultural Engineering
Mokhtar Ibrahim Dabbour
PhD
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About
85
Publications
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Introduction
Mokhtar Dabbour is an academic advisor, researcher and lecturer at the Department of Agricultural and Biosystems Engineering, Faculty of Agriculture, Benha University, Egypt. Dabbour does research in Food Processing and Engineering using novel and emerging processing technologies (e.g. Ultrasound technology). His research focus is also on shelf life extension and reduction of postharvest losses in vegetables and fruits. Dabbour is ready to collaborations and new ideas.
Publications
Publications (85)
Impacts of localized enzymolysis and sonication on physical, techno-functional, and structure attributes of sunflower meal protein (SMP) and its hydrolysate (SMPH) were studied. SMP was subjected to enzymolysis (using alcalase) to prepare SMPH with various degrees of hydrolysis (6 – 24% DH). Enzymolysis decreased colour lightness, turbidity, and pa...
Functionalities, conformational characteristics and antioxidative capacities of sunflower meal protein isolate (SMPI) and its hydrolysates (SMPIH) at various degree of hydrolysis (DH) (6, 12, 18, 24%) were investigated following sonication. Enzymolysis notably enhanced the solubility, foaming properties and emulsion stability index (ESI) of untreat...
Proteolysis kinetics and structure attributes of sunflower meal protein (SMP) pretreated using sonication of frequency type single (20, 40 kHz) and dual (20/40 kHz) were examined. A simplified model with impeded enzyme reaction was developed and the proteolysis of SMP in a heterogeneous system was successfully described. Initial observed rate (kin)...
Effect of mono and dual frequency (20, 28 and 20/28 kHz) ultrasound action on extraction kinetics, functional, and structural traits of sunflower protein (SP) was examined. A simplified model based on Fick's second law was developed, and the mass transfer of soluble SP in a heterogeneous system was successfully described. Findings suggested that ul...
Cottonseed meal (CM) is a useful protein resource, but the presence of gossypol (a toxic compound) limits it utilization in industry. To reduce the gossypol content, the use of ultrasonication in extracting protein from CM was explored. Following single-factor experiments, operative limits of independent variables affecting removal
ratio of total-g...
This study focused on enhancing the production of gamma-aminobutyric acid (GABA) by Lactiplantibacillus plantarum using ultrasound-assisted fermentation. L. plantarum with high GABA-producing capacity was isolated from Chinese pickles, and the optimized ultrasonic conditions were achieved after 9 h of seed inoculation at 28 kHz for 40 min. Compared...
pH-shifting (pH 1.5, 3.5, 9.5, 11.5) and sonication were exclusively and synergistically used to produce cottonseed meal protein (CSMP) with modified structure and improved functionality. Conventionally extracted CSMP served as control. Combined pH-shifting and sonication considerably reduced the β-sheet (40 – 27 %), but increased random coil (12 –...
Lysinoalanine (LAL) formed during alkaline extraction of rice residue protein (RRPI), which limited its application in the food industry. In this study, the influence of ultrasonication parameters (acoustic power density, ultrasound duration, and ultrasound temperature) on the inhibition of LAL formation and conformational attributes of RRPI during...
The aim of this study was to develop stabilized nanocomplexes of peach gum polysaccharide (PG) and sodium caseinate (SC) and evaluate the influence of high-intensity ultrasonication (ULS) on their structural and emulsification characteristics. The structural arrangement of sonicated PG-SC nanocomplexes ((PG-SC)U) was ascertained through Raman spect...
Abstrac
Female adult Eupolyphaga sinensis Walker (FAESW) has traditionally been a food source in Southeast Asian countries such as China and India, due to its rich nutritional content. However, the nutritional value of male adults (MAESW) and its molts (MESW) has hardly been reported. Therefore, this study aims to explore the potential application...
The bitterness of soy protein isolate hydrolysates prepared using five proteases at varying degree of hydrolysis (DH) and its relation to physicochemical properties, i.e., surface hydrophobicity (H0), relative hydrophobicity (RH), and molecular weight (MW), were studied and developed for predictive modelling using machine learning. Bitter scores we...
Fried seabass fish skin is one of the favorite snack products in Asian countries. This study was aimed to examine the influence of ultrasonic pretreatment and varying air-frying temperatures on the dehydration and thermodynamic characteristics of fried seabass fish skin product. Additionally, the research sought to assess mathematical models for un...
The functionality of rapeseed meal is limited, to acquire more utilization, the functional attributes were improved by altering its structural features using magnetic field-assisted solid fermentation. The magnetic treatment was performed every 24 h (specifically at 24, and 48 h), each treatment having a duration of 4 h. The magnetic intensity was...
High-concentration soy protein isolate was subjected to ultrasonication for viscosity reduction to assist the process of limited enzymatic hydrolysis. Ultrasonication (20 kHz, 10 min, 160 W/L) effectively reduced the viscosity of soy protein isolate at a comparatively high concentration of 14 % (w/v) and promoted the limited enzymatic hydrolysis (c...
BACKGROUND
There has been a significant growth in demand for plant‐derived protein, and this has been accompanied by an increasing need for sustainable animal‐feed options. The aim of this study was to investigate the effect of magnetic field‐assisted solid fermentation (MSSF) on the in vitro protein digestibility (IVPD) and functional and structur...
Moisture adsorption and desorption isotherms of gelatin extracted from whitefish skin powder (FSGP) at different temperatures across a wide range of water activity were determined along with their thermodynamic properties. Nine mathematical models were utilized for fitting the experimental data and simulating the adsorption and desorption behavior....
The effect of pectin concentration on the structural and emulsifying properties of black soldier fly larvae albumin
(BSFLA) modified by pH-shifting (pH12) and ultrasound (US) was studied. The results (intrinsic fluorescence,
surface hydrophobicity, Fourier transform infrared spectrum, and disulfide bonds) showed that modified BSFLA
samples, especia...
The effect of low-intensity fixed-frequency continuous ultrasound (LIFFCU) on the growth of Bacillus licheniformis YYC4 was investigated. The changes in morphology and activity of the organism, contributing to the growth were also explored. Compared with the control, a significant increase (48.95%) in the biomass of B. licheniformis YYC4 (at the lo...
This study focused on the influence of convection (at 40 °C and 50 °C) and freeze drying on functionality, structural properties and antioxidant potential of sunflower meal protein (SPm) and hydrolysate (SPHm). Likened to the respective convectively-dried isolates, lyophilized SPm and SPHm showed higher dispersibility and water binding efficacy, bu...
This study aimed to investigate the influences of mono-ultrasound assisted thawing on the thawing efficiency, product quality and conformational characteristics of frozen goose meat. The thawing time, thawing loss, muscle quality, and microstructure of frozen goose meat were studied. The results displayed that ultrasonic-assisted thawing effectivel...
The off-odors associated with spoilage of acidic beverages are linked to the germination and growth of Alicyclobacillus acidoterrestris (AAT) spores. As a consequence, we determined the influence of nutrients, non-nutrient germinants, dual-frequency thermosonication (DFTS), and food matrix on spore germination. AAT spores in orange juice (OJ), supp...
The application of silkworm pupa protein isolates (SPPI) in food industry was limited because SPPI's solubility is poor and it contains a potential harmful component of lysinoalanine (LAL) which formed during protein extraction. In this study, combined treatments of pH shift and heating were performed to improve the solubility of SPPI and to reduce...
Vibrio mimicus is a zoonotic pathogen that is widely distributed in aquatic habitats/environments (marine coastal water, estuaries, etc). The development of biocontrol agents for V. mimicus is imperative for the prevention and control of aquatic animal diseases and human food-borne infections. In this study, a broad-spectrum bacteriophage Vmp-1 was...
The majority of acidic fruits are perishable owing to their high-water activity, which promotes microbial activity, thus exhibiting metabolic functions that cause spoilage. Along with sanitary practices, several treatments are used during processing and/or storage to inhibit the development of undesirable bacteria. To overcome the challenges caused...
The effect of low-intensity sonication treatment on cell growth, ε-polylysine (ε-PL) yield and its biological mechanism were investigated, using a 3-L-jar fermenter coupled with an in situ ultrasonic slot with a Streptomyces albulus strain SAR 14-116. Under ultrasonic conditions (28 kHz, 0.37 W cm−2, 60 min), a high biomass of SAR 14-116 and concen...
Conventional fermentation of rapeseed meal has disadvantages such as sterilization requirement, high energy consumption and low efficiency, as well as poor action of single bacteria. To overcome these drawbacks, mixed-strain fermentation of unsterilized rapeseed meal was investigated. Mixed-fermentation of unsterilized rapeseed meal (ratio of solid...
Due to the recent increase in the human population and the associated shortage of protein resources, it is necessary to find new, sustainable, and natural protein resources from invertebrates (such as insects) and underutilized plants. In most cases, compared to plants (e.g., grains and legumes) and animals (e.g., fish, beef, chicken, lamb, and por...
Beef meatball (BM) is a traditional delicious snack with rich nutrition and unique flavor, making it a preferred choice for most consumers. However, the quality of BM is easily affected by many factors, such as the processing, storage, and preservation, which limit the competitive positioning with respect to its market. Therefore, it is essential t...
This review assesses the nonthermal physical processing technologies for protein modification. The review also discusses the structural, functional, and digestibility attributes of protein following nonthermal physical processing. Applications of ultrasonication, ultrahigh pressure, and pulsed electric field (PEF) can induce the conformational chan...
Alicyclobacillus acidoterrestris (AAT) was proposed as an index of pasteurization design for high-acid fruit products due to its spore resistance and repeated spoilage incidences in fruit juices. This study aimed to determine the effectiveness of pulsed multifrequency ultrasound to minimize AAT spores and vegetative cells in aqueous suspension. For...
Studies have shown that the disulfide bond conjugate formed by allicin (TS) and whey protein isolate (WPI) could significantly enhance the stability of TS. Based on this, the reaction conditions of TS and WPI were optimized, and the influence of binding rate on the molecular structure, rheological properties and microstructure of the conjugates was...
Air Frying technology is an innovative technique which could produce healthy fried food materials using hot air. The current work aimed to model the kinetic changes in mass transfer, texture and colour attributes and determining the temperature dependency of the rate constants of the mass transfer, texture and colour traits of the air-fried falafel...
The influence of freeze and convection (at 40 and 50 °C) drying on the physical, functional, and rheological attributes of sunflower protein (SP) and its hydrolysate (SPH) was investigated. Compared with convectively-dried samples, the lightness, turbidity, bulk density, and particle size values of the freeze-dried SP and SPH were substantially hig...
Development of an efficient liquid fermentation method is helpful for food and pharmaceutical applications. This study investigated the effect of ultrasonication on the liquid fermentation of Ganoderma lucidum, a popular edible and medical fungi. Significant changes at both metabolic and transcriptional levels in mycelia were induced by ultrasound...
Air-frying is an innovative technique for food frying that uses hot air circulation to prepare healthy products. The objectives of this study were to establish simplified models to reflect the efficacy of the air frying process at varying temperatures and times on the quality attributes of falafel, and to optimize the frying conditions for producin...
BACKGROUND
Fermentation efficiency of thermophiles of Bacillus licheniformis YYC4 and Geobacillus stearothermophilus A75, and mesophilic Bacillus subtilis 10 160 on soybean meal (SBM), was evaluated by examining the nutritional and protein structural changes.
RESULTS
SBM fermentation by B. licheniformis YYC4, B. subtilis 10 160 and G. stearothemop...
In this study, the effect of sonication on the fermentation process of a single-celled fungus was examined. During the experiment, Saccharomyces cerevisiae (S. cerevisiae) was used as the starting strain for ethanol fermentation (batch fermentation) in a 7.5 L automated fermentation tank. The fermentation tank connected with a six-frequency ultraso...
The influence of varying frequency modes of a low-power density ultrasound (LPDU) on the enzymolysis efficacy and structural property of corn gluten meal (CGM) was investigated. Sonication pretreatment (of CGM) with sequential and simultaneous duple-frequency modes enhanced notably the relative enzymolysis efficiency, compared to other LPDU frequen...
The use of ultrasound has the potential of improving the production of valuable substances
in Ganoderma lucidum (G. lucidum)—a well-known fungus rich in bioactive
compounds. The effect of different ultrasonic parameters on the fermentation of
G. lucidum was studied. The results showed that under optimal ultrasonic conditions,
the mycelium biomass a...
The influences of multi-frequency countercurrent S-type ultrasound (MFSU), with various frequency modes, on lysinoalanine (LAL) formation and conformational characteristics of rice dreg protein isolates (RDPI) were investigated. The ultrasonic operating mode with dual-frequency combination (20/40 kHz) indicated lower LAL content and higher protein...
Effects of atmospheric and room temperature plasma (ARTP) mutagenesis on Streptomyces albulus were investigated at cellular and molecular levels to elucidate the intracellular metabolism changes and the mechanism of ε-polylysine (ε-PL) yield enhancement in the mutant strain SAR 14−116 with ARTP treatment. An ε-PL yield increase by 18.46 % was obtai...
We investigated the effect of ultrasound-assisted pH shift treatment on the micro-particle, molecular, and spatial structure of rapeseed protein isolates (RPI). Various ultrasonic frequency modes (fixed, and sweep) was used. Protein characterization by the indexes: particle size, zeta potential, sodium dodecyl sulfate polyacrylamide gel electrophor...
The effect of conventional (control), and different ultrasonic treatment modes (fixed, and sweep frequency ultrasound) on structural, physical and functional traits of soldier fly (Hermetia illucens) larvae protein preparations and hydrolysates were examined. Ultrasonication significantly increased lightness (L*) of protein isolates by x¯ 7.46% com...
The effect of dual-frequency ultrasound treatment with different working modes on the lysinoalanine (LAL) formation and structural characterization of rice dreg protein isolates (RDPI) was studied during alkaline exaction processing. Ultrasonic notably decreased the LAL amount of RDPI and enhanced the protein dissolution rate. The LAL content of RD...
Impacts of dual‐frequency ultrasonic (DFU) with various operating modes (simultaneous and sequential modes) on degree of hydrolysis (DH) and conversion rate (CR) of sunflower meal protein (SMP) and activity of ACE‐inhibition of SMP hydrolysates were examined. Results revealed that sequential and simultaneous modes enhanced DH by 7.73% and 11.22%, a...
The influences of dual-frequency slit ultrasound (DFSU) pretreatment with various working parameters on the enzymolysis efficiency and conformational characteristics of corn gluten meal (CGM) were studied. Results indicated that under the conditions of ultrasonic power density of 80 W/L, time of 30 min, ultrasonic intermittent ratio of 5:2 s/s, tem...
This study sought to establish an optimized procedure for extraction of protein from microwave‐dried larvae of an edible insect, Hermetia illucens (HIL), and to determine and compare the amino acid (AA) component, intrinsic fluorescence (IF), and physicochemical/functional properties (PFPs) of protein isolates. Box–Behnken's design with the Box–Wil...
The instability of allicin makes it easily decomposed into various organic sulfur compounds, resulting in significant decrease in biological activity. In this study, allicin was firstly extracted with water, then bound with whey protein isolates (WPI) which were pretreated by ultrasound to form conjugates, and the stability, water solubility and em...
The main objective of this research was to monitor variety in the content of Bacillus subtilis 10160 during solid‐state fermentation (SSF) of rapeseed meal by near infrared (NIR) spectroscopy and chemometrics. Observations showed the coefficient of determination (R2) value was 0.9401 and root‐mean‐square deviation was 0.639 log (CFU/g) for viable c...
To reduce the high cost caused by steam sterilization of fermentation substrates and equipments in mesophilic solid-state fermentation of rapeseed meal (RM), a thermophilic protease-producing strain RM-2 from RM was isolated and employed in thermophilic solid-state fermentation of unsterilized RM for polypeptide preparation. The isolate was identif...
We investigated the impact of sonochemical action and the reaction of Hermetia illucens larvae meal protein (HILMP) as regards enzymolysis under varied enzyme concentration and temperature to explain the mechanism and effect of sonication on molecular conformation, limits of kinetics, free‐Gibbs energy, and antioxidative capacity. Control treatment...
Hermetia illucens (edible insect) larvae protein, and hydrolysates were prepared using three pretreatment modes (conventional, fixed-frequency ultrasonic, and sweep-frequency). Protein subunit scores, microstructure, antioxidative activity, and techno-functional property of the respective isolates and hydrolysates were investigated. Alkaline protea...
Hermetia illucens (edible insect) larvae protein, and hydrolysates were prepared using three pretreatment modes (conventional, fixed-frequency ultrasonic, and sweep-frequency). Protein subunit scores, microstructure, antioxidative activity, and techno-functional property of the respective isolates and hydrolysates were investigated. Alkaline protea...
The study investigated the effect of sonication conditions on antioxidant activity
of Hermetia illucens larvae meal protein hydrolysates. Three‐factor three‐level: pH
(7–9), time (10–30 min), and temperature (25–55°C) were optimized. Box–Behnken's
design was applied to optimize sonication treatment. Ferrous ion chelating activity
(ICA), DPPH‐radica...
To improve the utility of walnut meal protein, influences of ultrasonication on the kinetic and thermodynamic constraints of enzyme (trypsin) hydrolysis were assessed using a multi‐frequency counter current S‐type ultrasound machine. Results revealed that both the ultrasonication treatment and control hydrolysis obeyed the first‐order kinetics with...
Functionalities, conformational characteristics and antioxidative capacities of sunflower meal protein isolate (SMPI) and its hydrolysates (SMPIH) at various degree of hydrolysis (DH) (6, 12, 18, 24%) were investigated following sonication. Enzymolysis notably enhanced the solubility, foaming properties and emulsion stability index (ESI) of untreat...
In this study, ultrasound pretreatments of sunflower‐meal protein (SMP) to yield high antioxidant capacity from its hydrolysates by response surface methodology were optimized. Optimization of experimental conditions was achieved to examine the impact of temperature, solvent‐solid ratio and sonication time on antioxidant capacities of SMP hydrolysa...
In this study, the influences of ultrasonic treatments with different working modes (fixed frequency and sweeping frequency) and various frequencies on the bacterial growth and metabolism yield of Saccharomyces cerevisiae (S. cerevisiae) were investigated by employing an in situ ultrasonic irradiation slot coupled with a flask fermentation equipmen...