Mojtaba Tahmoorespur

Ferdowsi University Of Mashhad | FUM · Department of Animal Sciences

Long non-coding RNAs (lncRNAs) compose a plentiful category of transcripts that have gained increasing importance because of their roles in different biological processes. Although the function of most lncRNAs remains unclear. They are implicated in epigenetic regulation of gene expression, including muscle development and differentiation. We aimed...

The use of anticancer peptides (ACPs) as an alternative/complementary strategy to conventional chemotherapy treatments has been shown to decrease drug resistance and/or severe side effects. However, the efficacy of the positively-charged ACP is inhibited by elevated levels of negatively-charged cell-surface components which trap the peptides and pr...

This study was carried out to investigate the effect of age at first calving (AFC) on calving interval (CI) and dry period length (DP) of dairy cows in semiarid climate of Iran. Records of 57870 first lactation dairy cows were collected from 601 herds during 1995 to 2009 by the Animal Breeding Center of Iran. The analysis was performed using mixed...

Objective Salmonella is a Gram-negative, anaerobic, flagellated bacterium of Salmonella type in Enterobacteriaceae family, which is known as a zoonosis infectious agent. In recent years, different types of antibiotics have been used to overcome the Salmonella infection; however, the increasing number of antibiotic-resistant bacteria, the provision...

Antimicrobial peptides (AMPs) are native and safe short peptides with valuable biological effects. Nowadays, these components and their importance have attracted the attention of many researchers to determine their mode of action. Computational peptide engineering can donate a useful insight for investigating the stability and potency of AMPs. In t...

In the present study, a novel chimeric peptide was derived from camel lactoferrin designed with a considerable anti-HCV activity and its neutralization mechanism was predicted by molecular modelling tools. A novel anti-HCV peptide derived from camel lactoferrin (cLF36) was designed and expressed it recombinantly in HEK-293-T cells. Anti-viral activ...

Considering the fact that coronavirus is one of the major causes of calf diarrhea syndrome, this study aimed to investigate the interaction between the peptide cLF36 and the coronavirus protein by utilizing computational methods. Coronavirus amino acid sequence was obtained and molecular docking between coronavirus and the engineered camel lactofer...

Considering the fact that coronavirus is one of the major causes of calf diarrhea syndrome, this study aimed to investigate the interaction between the peptide cLF36 and the coronavirus protein by utilizing computational methods. Coronavirus amino acid sequence was obtained and molecular docking between coronavirus and the engineered camel lactofer...

To evaluate the effects of some environmental factors on the probability of being pregnant after only one insemination in Iranian Holstein cows, a total of 38074 records obtained from 10726 cows at different parities and inseminated during years 1985 to 2009 were utilized. All records were collected from a very large dairy herd which comprised of t...

To study the effect of non-genetic factors on survival of Karakul lambs from birth to weaning age 6,426 records collected from year 1992 through 2009 were used. Traits under study were cumulative survival from birth to 14, 30, 60 and 90 days of age. For each trait a linear model fitted which included the factors of lambs' year and month of birth, t...

Information about mortality of 6426 lambs which were born from 223 rams and 1769 ewes from years 1991 to 2015 in Sarakhs’ Karakul sheep breeding station was used. The trait under study was lambs’ monthly cumulative survival rate from time of birth to one year of age. Frequency of mortality causes and survival distribution function were analyzed usi...

Background: Lactoferrampin (LFampin), Lactoferricin (LFcin), and LFchimera are three well-known antimicrobial peptides derived from Lactoferrin and proposed as alternatives for antibiotics. Although the intracellular activity of these peptides has been previously demonstrated, their mode of action is not yet fully understood. Here, we performed a...

Background: Lactoferrampin (LFampin), Lactoferricin (LFcin), and LFchimera are three well-known antimicrobial peptides derived from Lactoferrin and proposed as alternatives for antibiotics. Although the intracellular activity of these peptides has been previously demonstrated, their mode of action is not yet fully understood. Here, we performed a m...

The Canarypox virus (CNPV) infects captive and wild canaries and cause high mortality and substantial economic losses especially in Middle East countries. Currently, unapproved and illegally imports of embryo propagated, freeze-dried, and live CNPV vaccines are being used by individuals for personal uses in Iran and the region against canarypox dis...

One major issue in cancer immunotherapy is the large size of mAbs (150 kDa) which usually causes limitation in tumor penetration. Camel nanobodies (< 15 kDa) could be used as an alternative for this problem. Here in, using molecular modeling we designed a novel recombinant chimeric protein containing the extra membrane loop of human CD20 which was...

Background Designing a potent recombinant vaccine, using the appropriate subunits with the greatest effect on stimulating the immune system, especially in the case of intracellular pathogens such as gram negative Brucella Melitensis bacteria, is of great importance. In this study, three repeats of 27 amino acids of the immunogenic epitope derived f...

Introduction: Native chicken breeds are important genetic resource and well adapted to the local environmental conditions. However, growth rate and feed efficiency of these breeds are not appropriate. On the other hand modern broilers grow faster and offer a higher nutritional efficiency than the indigenous chicken breeds. This advantage is the res...

Molecular dynamics (MD) simulation is the knowledge that combines biological science, chemistry, and various computer mathematical methods and algorithms. Simulation of molecular dynamics (MD) is a knowledge that a combination of biological sciences, chemistry and mathematical methods and algorithms computer variety. This advanced technique is used...

Objective: Two critical points of early development are the first and second lineage segregations, which are regulated by a wide spectrum of molecular and cellular factors. Gene regulatory networks, are one of the important components which handle inner cell mass (ICM) and trophectoderm (TE) fates and the pluripotency status across different mammal...

Unfortunately, using the antibiotics to treat mastitis is increasing in which leads to undesirable side effects. One of the antibiotics alternatives with high potential are antimicrobial proteins like lactoperoxidase. The aim of this study was to predict the peptides of lactoperoxidase protein and compare them in six different species (human, camel...

Background: Lactoferrampin (LFampin), Lactoferricin (LFcin), and LFchimera are three well-known antimicrobial peptides derived from Lactoferrin and proposed as alternatives for antibiotics. Although the intracellular activity of these peptides has been previously demonstrated, their mode of action is not yet fully understood. Here, we performed a m...

Brucellosis is an infectious disease caused by different Brucella species. The outer membrane proteins 25 and 31 play a significant role in stimulation of immunity against Brucella. Herein, the humoral and cellular immune responses of selected recombinant proteins emulsified in chitosan nanoparticles as individual (univalent) and simultaneous (diva...

Nowadays antimicrobial peptides are the most important alternative to antibiotics. However, the mechanism of the AMPs is not completely understood. In our previous studies, CLF36 as a chimeric derived peptide from camel lactoferrin showed a broad-spectrum antimicrobial activity against both Gram-negative and Gram-positive bacteria. To predict the m...

Previous studies on vaccine development against foot-and-mouth disease (FMD) virus reported that application of the inactivated vaccines for FMD virus is not completely effective. Novel vaccinations based on immune-dominant epitopes showed they induced immune responses. In addition, for better and safer immunization, access to of efficient adjuvant...

Objectives: The results of studies on vaccine development for foot-and-mouth disease (FMD) virus show that the use of inactivated vaccines for FMD virus is not completely effective. Novel vaccinations based on immuno-dominant epitopes have been shown to induce immune responses. Furthermore, for safety of immunization, access to efficient adjuvants...

Objectives: Brucellosis is a common infectious disease among animals and humans. While subunit vaccines could be used as an efficient strategy against pathogens, they usually seem to be less immunogenic than live or killed vaccines. However, the use of a suitable adjuvant accompanied by subunit vaccines can be a good alternative to enhance the imm...

Background Using a bacterial expression system such as Escherichia coli (E. coli) is very common for protein expression because of its simplicity, low cost and high efficiency. Objective In order to express proteins that contain di-sulfide bands, an oxidative space such as the periplasmic environment of the bacteria is required. Therefore, a leade...

Native chicken breeds are important genetic resource and well adapted to the local environmental conditions. However, growth rate and feed efficiency of these breeds are not appropriate. Comparative studies of commercial and native chickens provide an opportunity to characterize the biological basis of differences between them. Here, RNA-Seq technol...

s The factors that affect residual feed intake (RFI) of two chicken breeds (Ross as commercial and Isfahan as Iranian local breed with low and high RFI, respectively) were examined. We sequenced their liver transcriptomes using RNA-Seq while focusing on the identification of important candidate genes, which might influence RFI and growth rate. The...

Brucellosis is a zoonotic disease in human and animals. Brucella melitensis is one of the most pathogenic species of Brucella in goat and sheep. Omp31 is an outer membrane protein of Brucella that acts as an immunogenic protein. Cytokines are glycoproteins with low molecular weight that play the role of an immune adjuvant and regulate immune respon...

Genomic prediction using a large number of markers is challenging, due to the curse of dimensionality as well as multicollinearity arising from linkage disequilibrium between markers. Several methods have been proposed to solve these problems such as Principal Component Analysis (PCA) that is commonly used to reduce the dimension of predictor varia...

Objective(s) Vaccination is one of the most effective means to protect humans and animals against brucellosis. Live attenuated Brucella vaccines are considered effective in animals but they may be potentially infectious to humans, so it is vital to improve the immunoprotective effects and safety of vaccines against Brucella. This study was designed...

Objectives: The Streptomyces phage phiC31 integrase offers a sequence-specific method of transgenesis with a robust long-term gene expression. PhiC31 has been successfully developed in a variety of tissues and organs for purpose of in vivo gene therapy. The objective of the present experiment was to evaluate PhiC31-based site-specific transgenesis...

Brucellosis is one the serious infectious diseases caused deleterious health and economic losses. Vaccination with subunit vaccines is the efficient alternative way than live attenuated vaccines against infectious diseases. Herein a new chimeric OMP25-BLS antigen emulsified in Chitosan Nanoparticles was designed and its immune responses were compar...

Brucellosis caused by the bacterium Brucella affects various domestic and wild species. The outer membrane proteins 25 and 31 play key roles on stimulation of cell-mediated immune response against Brucella. GroEL as one of the major Brucella antigens stimulates the immune system and increases intracellular survival of bacteria. In the present study...

The randomly amplified polymorphic DNA technique was developed by Williams et al,(1990) and Welsh and McClelland et al.(1990). The technique is based on polymerase chain reaction using primers homologous to random target sites in the genome. The main advantages of RAPD assays are that it is simple, less labour intensive, comparatively less expensiv...

Protective antigen (PA), a subunit of anthrax toxin from Bacillus anthracis, is known as a dominant component in subunit vaccines in protection against anthrax. In order to avoid the side effects of live attenuated and killed organisms, the use of linear neutralizing epitopes of PA is recommended in order to design recombinant vaccines. The present...

Brucellosis is caused by the bacterium Brucella and affects various domestic and wild species. GroEL (Heat Shock Protein 60kDa) as one of the major antigens that stimulate the immune system, increases Brucella survival. The aim of the current study was to clone and express GroEL in Escherichia coli in order to design subunit vaccine. Amplifying was...

This investigation aimed to study polymorphism of growth hormone (GH), growth hormone receptor (GHR), and insulin like growth factor 1 (IGF-1) genes and their associations with growth and carcass traits in Mazandaran native chicken. 200 male chicks were reared and slaughtered at 12 weeks of age. Traits including live weight at 4, 8 and 12 weeks of...

The poultry industry occupies an important position in the provision of animal protein. Recently, next generation sequencing technology (RNA-Seq) has become available as a powerful tool to investigate transcriptional profiles for gene expression analysis of many organisms. The main use of RNA-Seq in agriculture species are focusing on finding the i...

Brucellosis is a well-known infection in domestic animals which caused by Brucella bacterium. Due to serious economic and medical consequences of this disease, various efforts have been made to prevent the infection through the use of recombinant vaccines based on Brucella outer membrane protein (OMP) antigens. The objectives of the present study w...

The aim of current study was to investigate the nucleotide sequence of seven tRNA genes in mitochondrial gene of Khorasan’s native chicken and identification of possible mutations in it. For performing it, this part of the gene was cloned using the specific amplification initiators and cloned in the plasmid pTG19-T vector in DH5? strain of E-Coli a...

Background Little is understood about the regulation of gene expression during early goat embryo development. This study investigated the expression profile of 19 genes, known to be critical for early embryo development in mouse and human, at five different stages of goat in vitro embryo development (oocyte, 8-16 cell, morula, day-7 blastocyst, and...

Brucellosis is a well-known domestic animal infectious disease, which is caused by Brucella bacterium. The outer membrane protein 25 kDa (Omp25) gene plays an important role in simulating of TNF-α, IFN-α, macrophage, and cytokines cells. In the current study molecular cloning and expression analysis of Omp25 gene for designing a subunit vaccine aga...

Over the past two decades a large group of natural compounds with low molecular weight was extracted from plants and animals that called antimicrobial peptides and showed antimicrobial activities. Scientists state these compounds could be classified as an alternative to chemical antibiotics. Cationic peptides are widely known among the antimicrobia...

Lactoferrin (LF) is a globular glycoprotein present in milk with a molecular mass of about 80 kDa. LF has many peripheral functions, inducing primary defense against bacterial and viral infection, antitumor activity, immunomodulation, and cell growth regulation. Lactoferrin is a rich source of cationic and hydrophobic antimicrobial peptides, which...

Abstract mastitis have a different effects on industry's costs and health, replacing antibiotics with compounds without side effects is very important. In this study, Salmonella and coliform bacteria was extracted from cows mastitis and the effects of recombinant camel lactoferrin peptide inhibitory on the growth and proliferation of this bacteria...

Bovine Rotavirus and Bovine Coronavirus are the most important causes of diarrhea in newborn calves and in some other species such as pigs and sheep. VP8 subunit of rotavirus is the major determinant of the viral infectivity and neutralization. Spike glycoprotein of coronavirus is responsible for induction of neutralizing antibody response. Studies...

Objective(s): Enterotoxigenic Escherichia coli (ETEC) strains are one of the primary causes of diarrhea in newborn calves and in humans, pigs, and sheep. IgY technology has been identified as a promising alternative to generating a mass amount of specific antibody for use in immunotherapy and immunodiagnostics. The purpose of this study was to prod...

Brucellosis, caused by the genus Brucella bacterium, is a well-known infection among domestic animals. Considering the serious economic and medical consequences of this infection, various preventive efforts have been made through using recombinant vaccines, based on outer membrane protein (OMP) antigens of Brucella species. The objective of the pre...

Foot-and-mouth disease (FMD) is a severely contagious viral disease that mainly affects cloven-hoofed livestock and wildlife. This study quantifies the cytokines mRNA expression of vaccinated guinea pigs with FMD type O inactivated vaccine. Blood samples were collected from eight guinea pigs at 7 and 28 days after the first vaccination. Extracted m...

Purpose: Bovine Rotavirus and Bovine Coronavirus are the most important causes of diarrhea in newborn calves and in some other species such as pigs and sheep. Rotavirus VP8 subunit is the major determinant of the viral infectivity and neutralization. Spike glycoprotein of coronavirus is responsible for induction of neutralizing antibody response....

Insulin-like growth factor gene (IGF-1) is one of the most important growth factors that plays a key role in the proliferation and differentiation of muscle cells. IGF-1 is also a radial stimulant in muscle hypertrophy in mammals. In our study, we constructed a lentiviral vector inducing an overexpression of IGF-1 in order to study the regulating m...

Recombinant lentivirus expression vector for insulin-like growth factor (IGF-1) gene delivery, Onl J Vet Res., 20(8):563-570, 2016. Insulin-like growth factor gene (IGF1) affects animal growth. DNA IGF1 was used as transfer vector to produce recombinant IGF1 in mammalian cells. Transfer and control vector were co-transfected into HEK293T cell line...

Kappa-casein, as a major protein component in mammalian milk, plays an essential role in formation and stabilization milk micelles and preventing them from aggregating and therefore, helping to keep calcium phosphate in solution and transfer of calcium and phosphors from animal milk to consumers. Therefore, the objective of the current study was to...

Due to uncontrolled crosses, the genetic purity of sheep breeds has changed. Therefore, using of genetic markers of mitochondrial sequencing is one the ways to study these changes. This experiment carried out to evaluate the diversity of HVR-I region from mtDNA in Afshari sheep breed. For conducting this study, the blood samples were collected from...