Mohd Nazri Ismail

Mohd Nazri Ismail
Universiti Sains Malaysia | USM · Analytical Biochemistry Research Centre (ABrC)

PhD

About

57
Publications
6,475
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
499
Citations
Citations since 2017
39 Research Items
285 Citations
20172018201920202021202220230204060
20172018201920202021202220230204060
20172018201920202021202220230204060
20172018201920202021202220230204060
Additional affiliations
January 2016 - present
Universiti Sains Malaysia
Position
  • Professor (Associate)
January 2011 - December 2015
Universiti Sains Malaysia
Position
  • Lecturer
Description
  • Re branded to Analytical Biochemistry Research Centre
Education
June 2007 - December 2010
Imperial College London
Field of study
  • Biopolymer Mass Spectrometry
March 2002 - March 2005
Universiti Kebangsaan Malaysia
Field of study
  • Biochemistry

Publications

Publications (57)
Article
Background The advancement of proteomics studies leads to various benefits in research. However, in Malaysia, proteomics studies are still in their early stage. Objective Four adult Pulchrana glandulosa individuals were collected from the stream of Temurun Waterfall for their proteomics information. Methods These frogs’ skin secretions were colle...
Preprint
Full-text available
Hylarana erythraea lives in disturbed and undisturbed habitats, making them vulnerable to predators and pathogens. This study aims to compare skin secretion’s protein profile of H. erythraea from disturbed (Jetty Kuah and Kampung Bohor Chempedak) and undisturbed (Temurun and Telaga Tujuh waterfall) habitats using Orbitrap-Liquid Chromatography-Mass...
Article
Full-text available
Fish sauce is a well-known condiment for its delicious flavour developed through a fermentation process. It is generally used as an additive for flavour enhancement in cooking. The fish sauce comes in several appearances, ranging from a clear liquid with light colour and texture to a dark and cloudy liquid. This product is produced by a longduratio...
Article
Full-text available
Horseshoe crabs are one of the most studied invertebrates due to their remarkable innate immunity mechanism and biological processes. In this work, the proteins of the lipopolysaccharides (LPS)-stimulated and non-stimulated hemocytes of Malaysian Tachypleus gigas were profiled using LC-MS/MS. A total of 154 proteins were identified in both types of...
Article
Enzymatic activity and microbial fermentation play a prominent role in the bioconversion of complex muscle tissue into smaller units of peptides and amino acids, possibly contribute to sensory properties. Thus, this study screens and evaluate anchovy proteins with taste-active peptides and amino acids by the reaction of multiple enzymes using an in...
Article
High-fat diet (HFD) increases the risk of obese, while obesity increases the prevalence of metabolic syndrome and non-communicable diseases. Therefore, it will be interesting to evaluate the changes in metabolic parameters and brain profile upon the early consumption of HFD. In this study, a total of 12 Sprague-Dawley male rats were divided into tw...
Article
Hevea brasiliensis is one of the most important agricultural commodities globally, heavily cultivated in Southeast Asia. Fatal leaf fall diseases cause aggressive leaf defoliation, linked to lower latex yield and death of crops before maturity. Due to the significant consequences of the disease to H. brasiliensis, the recent gene expression studies...
Thesis
Amphibians are the most diverse terrestrial vertebrates and are essential for conserving the environment since they act as biological indicators. Furthermore, frogs were one of the primary sources of bioactive compounds for biomedical and pharmaceutical applications. A total of 24 species of amphibians from six families were documented within Langk...
Article
Full-text available
The skin secretion of amphibians is known for its high content of bioactive compounds. These bioactive compounds are essential for the advancement of biomedical industries. Four wild green paddy frogs, Hylarana erythraea, were collected from various habitat types within the Langkawi Archipelago. These frogs’ skin secretions were collected, extracte...
Presentation
Hylarana erythraea lives in disturbed and undisturbed habitats, making them vulnerable to various predators and pathogens. This study aims to compare skin secretion's protein profile of H. erythraea from disturbed (Jetty Kuah and Kampung Bohor Chempedak) and undisturbed (Temurun and Telaga Tujuh waterfall) habitat using Orbitrap-Liquid Chromatograp...
Article
Full-text available
The fruit and leaf of God’s crown (Phaleria macrocarpa) have been traditionally used to treat a wide variety of diseases. However, the proteins of this tropical plant are still heavily understudied. Three protein extraction methods; phenol (Phe), trichloroacetic acid (TCA)–acetone–phenol (TCA–A–Phe), and ultrasonic (Ult) were compared on the fruit...
Presentation
Full-text available
Biodiversity Of Amphibians In Langkawi Archipelago And The Study On Protein Composition From Skin Secretion Of Several Frog Species
Article
Full-text available
Rice bran, a by-product of the rice milling process, has emerged as a functional food and being used in formulation of healthy food and drinks. However, rice bran is often contaminated with numerous mycotoxins. In this study, a method to simultaneous detection of aflatoxins (AFB1, AFB2, AFG1, and AFG2), ochratoxin A (OTA), deoxynivalenol (DON), fum...
Article
Full-text available
Antimicrobial resistance (AMR) is a significant threat to human health globally. Diseases caused by AMR are getting more challenging to cure due to the increasing rate of resistance of microbial pathogens. Drugs formulated with antimicrobial peptide (AMP) can be applied to treat these diseases. AMPs are found naturally within organisms, especiall...
Article
Full-text available
Cancer stem cells (CSCs) represent a small subpopulation within a tumour. These cells possess stem cell-like properties but also initiate resistance to cytotoxic agents, which contributes to cancer relapse. Natural compounds such as curcumin that contain high amounts of polyphenols can have a chemosensitivity effect that sensitises CSCs to cytotoxi...
Conference Paper
Background: Breast cancer is the fifth most prevalent cause of death among women worldwide. It is also one of the most common types of cancer among Malaysian women. This study aimed to characterize and differentiate the proteome profiles of different stages of breast cancer and its matched adjacent normal tissues in Malaysian breast cancer patients...
Article
Natural rubber or latex from the Hevea brasiliensis is an important commodity in various economic sectors in today's modern society. Proteins have been detected in latex since the early twentieth century, and they are known to regulate various biological pathways within the H. brasiliensis trees such as the natural rubber biosynthesis, defence agai...
Article
High-fat diet (HFD) interferes with the dietary plan of patients with type 2 diabetes mellitus (T2DM). However, many diabetes patients consume food with higher fat content for a better taste bud experience. In this study, we examined the effect of HFD on rats at the early onset of diabetes and pre-diabetes by supplementing their feed with palm olei...
Article
Channa striata (also known as Channa striatus) is an air-breathing freshwater snakehead fish which is well known in the Asian region for its medicinal properties, particularly in wound healing. It is recognised as a traditional medicine (TM) which can be served as alternative treatment methods. With the advancement of technology, researchers have m...
Article
Full-text available
Background: Type 2 Diabetes is the most common form of diabetes mellitus, accounting for 90% of all types of diabetes. Diet is one of the important factors affecting the progression of the disease. Methods: In this study, we used urinary protein profile to evaluate the progression of pre-diabetic to diabetic state. Urinary protein profile of pre-di...
Article
Full-text available
Background Breast cancer is the fifth most prevalent cause of death among women worldwide. It is also one of the most common types of cancer among Malaysian women. This study aimed to characterize and differentiate the proteomics profiles of different stages of breast cancer and its matched adjacent normal tissues in Malaysian breast cancer patient...
Article
Channa striata are speculated to contains bioactive proteins with the ability to enhancing wound healing. It is commonly consumed after surgery for a faster recovery of the wound. To identify the bioactive proteins and evaluate their ability to promoting cell proliferation and angiogenesis. Material and Methods Freeze-dried water extracts (FDWE)...
Article
Objectives Reference values of hematologic parameters are imperative not only in clinical applications but also for monitoring of banned substances in sports. Since the values can be highly influenced by sexes, genetic origins and geographical factors, we assessed the haematological parameters of junior athletes from the Malaysian population. This...
Article
Recombinant human erythropoietin (rHuEPO) is the first cloned hematopoietic growth factor available for pharmaceutical treatment, especially anemia, since 1988. Unfortunately, the ability of rHuEPO in boosting erythropoiesis, and thus aerobic capacity has led to its abuse in endurance sport. Besides, the expiry of the original rHuEPO patent has res...
Article
Objective: Urinary steroids profiling was used to monitor the intake of endogenous anabolic androgenic steroids by athletes. However these profile can be influenced by different factors such as age, gender, ethnicity, bacterial and microorganism activities in urine. This paper discussed the difference of steroids profile between different populatio...
Poster
Full-text available
The freshwater snakehead fish, Channa striata is a popular local meal and often used as a traditional medicine for generations due to its ability to enhance wound healing. Pharmacological assays have been carried out to access the pro-proliferative and pro-angiogenic activity of the proteins available within. Four samples harvested from the snakehe...
Article
The proper method of harvesting breadfruit, Artocarpus altilis (Parkinson) Fosberg in commercial plantations often includes the process of draining the latex to avoid the staining of the epicarp. Therefore, this plant's exudate is not fully utilized and has no current agricultural use and only applied as traditional remedy for wound healing. Thus,...
Article
The natural rubber latex extracted from the bark of Hevea brasiliensis plays various important roles in today's modern society. Post-translational modifications (PTMs) of the latex proteins are important for the stability and functionality of the proteins. In this study, latex proteins were acquired from the C-serum, lutoids and rubber particle lay...
Article
Background: Channa striata is a freshwater snakehead fish that is abundantly available in South East Asia. Proteins available in the C. striata's mucus have been confirmed to contribute towards wound healing enhancement, yet the biologically active proteins within are poorly understood. Objective: The main objective was to identify the potential pr...
Article
Cathepsin L (CPL) cysteine protease is a proteolytic enzyme that involves in many biological processes in a wide range of organisms. In free-living nematode Caenorhabditis elegans, CPL plays important roles in embryogenesis and development processes. The CPL protein is also believed to have a role in degradation of blood meal in the gut of parasiti...
Article
Date fruits are well known to be very nutritious. Nevertheless, the protein content of the fruit, particularly the seed and flesh is still understudied, largely due to their difficult physical characteristics. This study was conducted to compare three different protein extraction methods which were the TCA-acetone (TCA-A), phenol (Phe), and TCA-ace...
Article
Full-text available
Background and Objective: Proteomics has been developed remarkably and become an intensive field of research; especially protein glycosylation which is considered one of the most common post-translational modifications. Glycosylated proteins play vital roles in several biological and molecular pathways and it represents over 50% of human proteins....
Article
Full Text: http://www.tandfonline.com/eprint/t8z5DY6edjtkP9D5fn2c/full Food contamination is a major global issue and the challenges are increasing. Alternative methodology using lipids in detecting contaminants and determining food ingredients is needed to supplement or replace current methodology. The core-shell column is considered to be a brea...
Article
Full-text available
The four-lined tree frog, Polypedates leucomystax, spawns its eggs in a moist structure called a foam nest. Four foam nests constructed by this species were collected from the Sungai Sedim Recreational Forest, Kedah, Peninsular Malaysia. Two foam nests were found deposited on the leaves of low vegetation hanging over a rock pool. One was attached i...
Article
Full-text available
Background Excretory-secretory (ES) proteins of E. histolytica are thought to play important roles in the host invasion, metabolism, and defence. Elucidation of the types and functions of E. histolytica ES proteins can further our understanding of the disease pathogenesis. Thus, the aim of this study is to use proteomics approach to better understa...
Article
The natural rubber latex extracted from the bark of Hevea brasiliensis plays various important roles in today's modern society. Following ultracentrifugation, the latex can be separated into 3 layers; C-serum, lutoids, and rubber particle. Previous studies showed that a large number of proteins were present inside these three layers. However, a com...
Article
Full-text available
Channa striatus is a freshwater snakehead fish that is commonly found in Malaysia. Several publications have reported the wound healing effect is available within the fish, yet the responsible biomolecules is yet to be clarified. In the current study, we have performed a comprehensive proteomic profiling of C. striatus meat using high sensitivity l...
Article
Full-text available
| Channa striatus is a carnivorous freshwater fish that is commonly consumed among Malaysians. The fish is known to contain compound(s) that can accelerate the wound healing process in humans, but the attributes of these compound(s) are yet to be clarified. In the current study, we have performed a thorough proteomic profiling of spray -dried and f...
Article
Full-text available
Erythropoietin (EPO), a hormone that regulates the synthesis of red blood cells, is frequently abused by athletes. Sodium dodecyl sulphate poly acrylamide gel electrophoresis (SDS-PAGE) is an essential analytical technique in all anti-doping laboratories in order to detect the abuse of EPO. An immunoaffinity purification step is now considered esse...
Conference Paper
Introduction: Glucocorticoid (GC) hormones are important to the development of the lung’s Na+-absorbing phenotype, however Na+ transport also subject to acute regulation via cAMP-coupled agonists. Previous works suggested that Na+ conductance is increased in favour of cAMP activation through PKA pathway (Ramminger et al. 2002, Clunes et al. 2004)....
Article
Full-text available
Core 2 β1,6-N-acetylglucosaminyltransferase (C2GnT), which exists in three isoforms, C2GnT1, C2GnT2 and C2GnT3, is one of the key enzymes in the O-glycan biosynthetic pathway. These isoenzymes produce core 2 O-glycans and have been correlated with the biosynthesis of core 4 O-glycans and I-branches. Previously, we have reported mice with single and...
Article
The three glycosyltransferases of the Core 2 beta1,6-N-acetylglucosaminyltransferase (C2GnT) family, C2GnT1, C2GnT2, and C2GnT3, are able to initiate the Core 2 branch of O-glycans. However, C2GnT2, which is highly expressed in the digestive tract, has a broader acceptor substrate specificity that allows it to also generate Core 4 O-glycans and I b...
Article
Full-text available
Mass spectrometry (MS) has proven to be the preeminent tool for the rapid, high-sensitivity analysis of the primary structure of glycans derived from diverse biological sources including cells, fluids, secretions, tissues, and organs. These analyses are anchored by matrix-assisted laser desorption ionization time of flight (MALDI-TOF) analysis of p...
Article
The three glycosyltransferases of the Core 2 β1,6-N-acetylglucosaminyltransferase (C2GnT) family, C2GnT1, C2GnT2, and C2GnT3, are able to initiate the Core 2 branch of O-glycans. However, C2GnT2, which is highly expressed in the digestive tract, has a broader acceptor substrate specificity that allows it to also generate Core 4 O-glycans and I bran...
Article
Full-text available
Glycoconjugates expressed on gastric mucosa play a crucial role in host–pathogen interactions. The FUT2 enzyme catalyzes the addition of terminal α(1,2)fucose residues, producing the H type 1 structure expressed on the surface of epithelial cells and in mucosal secretions of secretor individuals. Inactivating mutations in the human FUT2 gene are as...
Article
Full-text available
Three glycosyltransferases have been identified in mammals that can initiate core 2 protein O glycosylation. Core 2 O-glycans are abundant among glycoproteins but, to date, few functions for these structures have been identified. To investigate the biological roles of core 2 O-glycans, we produced and characterized mice deficient in one or more of...

Network

Cited By

Projects

Projects (8)
Project
To understand to contributions of proteins and peptides to the health benefits of various natural products. Main tools used are LC-MS/MS and MALDI-MS/MS.
Project
to explore the biomedical, proteomics and bioinformatics potentials of frog secretions