
Mohammed YoussefVeterinary medicine Faculty, South Valley University, Qena, Egypt · Animal Physiology
Mohammed Youssef
PhD
About
24
Publications
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Introduction
I am working as an associate professor of Physiology at South Valley University. I have an interest to investigate immune and inflammatory responses. I was a visiting research student at Kyushu University, and a post-doc research fellow at the Japanese National Institute for Physiological sciences. Recently, I made steps forward to learn data science and machine learning techniques as a cardinal skill in neuroscience and other real-world problems.
Publications
Publications (24)
Neuroinflammation characterized by activation of glial cells is observed in various neurodegenerative diseases including Alzheimer's disease (AD). Although the reduction of ether-type glycerophospholipids, plasmalogens (Pls), in the brain is reported in AD patients, the mechanism of the reduction and its impact on neuroinflammation remained elusive...
The special lipids plasmalogens (Pls) were reported to be reduced in the neurodegenerative brains such as Alzheimer’s disease where a marked increase of glial activation is often observed. We previously found that a reduction of brain Pls can enhance the glial activation in murine brains. However, the detailed role of Pls in the prevention of glial...
Feedback projections from the secondary motor cortex (M2) to the primary motor and sensory cortices are essential for behavior selection and sensory perception. Intratelencephalic (IT) cells in layer 5 (L5) contribute feedback projections to diverse cortical areas. Here we show that L5 IT cells participating in feedback connections to layer 1 (L1)...
In mammalian neocortex, learning triggers the formation and turnover of new postsynaptic spines on pyramidal cell dendrites. However, the biological principles of spine reorganization during learning remain elusive because the identity of their presynaptic neuronal partners is unknown. Here, we show that two presynaptic neural circuits supervise di...
The study of the geometric organization of biological tissues has a rich history in the literature. However, the geometry and architecture of individual cells within tissues has traditionally relied upon manual or indirect measures of shape. Such rudimentary measures are largely a result of challenges associated with acquiring high resolution image...
N-nitrosodiethylamine (ND) is an extremely toxic unavoidable environmental contaminant. Copper II-albumin (CuAB) complex, a newly developed Cu complex, showed antioxidant and anti-inflammatory potential. Hereby, we explored the plausible neuroprotective role of CuAB complex toward ND-evoked neurotoxicity in mice. Twenty-four male mice were sorted i...
Aloe Vera (AV) is a reputable herbal medical and feed additive. The gel extract of AV contains mucopolysaccharides that stimulate the innate immune response. The reaction to Aloe Vera administration might involve beneficial or drastic responses depending on the dose and extraction method. In the current investigation, two different concentrations o...
Ammonia is one of the most harmful water quality parameters restricting the growth and survival of aquatic living organisms. As a result, fish must adapt to this stressor by modifying physiological processes that are governed by gene expression regulation. The aim of the present study was to investigate the expression profiles of antioxidant relate...
Osteoarthritis (OA) is a degenerative disorder involving the joint, including cartilage and synovial fluids. Recent studies have sought to find curative therapeutics to decrease the adverse effects of OA and relieve associated pain. Globally, knee osteoarthritis (KOA) is the most common type of arthritis. The present study aimed to evaluate the ant...
Aflatoxin B1 (AF) is an unavoidable environmental pollutant that contaminates food, feed, and grains, which seriously threatens human and animal health. Arabic gum (AG) has recently evoked much attention owing to its promising therapeutic potential. Thus, the current study was conducted to look into the possible mechanisms beyond the ameliorative a...
Nitrosodiethylamine (NDEA) is a potent oxidant induces neurodegeneration via (reactive
oxygen species) ROS. Copper is an important metal essential for scavenging free radicals,
development of central nervous system (CNS) and redox angiogenesis signaling. Vascular
endothelial growth factor (VEGF) is well known as efficacious and long-term signal tha...
Amelioration of hyperinsulinemia and insulin resistance associated with obesity is a cardinal target for therapeutics. Therefore, we investigated the relation of Fibrilln-1 (FBN1) mRNA expression and hepatic phosphoenolpyruvate caboxykinase (PEPCK) enzyme to the ameliorative impact of oxytocin on obesity-induced diabetes, suggesting glycogenolysis...
Toxoplasma gondii is associated with physiological and psychiatric perturbations. The immune response is interrelated to the progress of anhedonia and despair symptoms of T. gondii-infected subjects. We recently reported that serum N-glycans were altered in mice displayed depressive-like behaviors. However, a novel biomarker that correlated to T. g...
Drugs that are commonly used in poultry farms can potentially cause a detrimental effect on meat consumers as a result of chemical residues. Therefore, seeking a natural alternative is crucial for the health of the consumers. The egg yolk immunoglobulin Y (IgY) is a promising natural replacement for antibiotics in the broilers' diet. There is a sca...
Feed additives are used frequently in variable combinations to maximize broiler productivity and consumer safety. Therefore, we evaluated the efficiency of feed additives used in four different diets: a basal diet, a probiotic (PRO-PAC®) supplement diet, an egg yolk purified immunoglobulin Y (IgY) supplemented diet, and a combination of IgY and PRO...
Toxoplasma gondii is associated with physiological and psychiatric perturbations. The immune response is interrelated to the progress of anhedonia and despair symptoms of T. gondii-infected subjects. We recently reported that serum N-glycans were altered in mice displayed depressive-like behaviors. However, a novel biomarker that correlated to T. g...
This study was carried out to compare the analgesic effects and haematobiochemical changes produced by the administration of lidocaine, detomidine and lidocaine–detomidine combination in the epidural space of goats. All goats were assigned to all of the three different epidural treatments using a balanced crossover design with two-weeks washout per...
Glycosylation is a post-translational protein modification in eukaryotes and plays an important role in controlling several diseases. N-glycan structure is emerging as a new paradigm for biomarker discovery of neuropsychiatric disorders. However, the relationship between N-glycosylation pattern and depression is not well elucidated to date. This st...
The ability of reduced graphene oxide (rGo) in regenerating bone tissue has recently gained significant attention in biomedicine. Aim: we investigated the effects of rGo nanoparticles and platelet rich plasma (PRP) in regenerating induced defects in irradiated 2 types of rat bones. Materials and Methods: We had 4 experimental rat groups, each was 6...
ARTICLE INFO In the last decades, the light had been shed on the importance of male reproduction and how to protect it from disease conditions and inflammation which may cause infertility. Accordingly, the mechanism underlying inflammation-mediated infertility must be well clarified. In the present study, an experimental model of acute inflammation...
Immunoglobulin Y (IgY) is beneficial to form the passive immunization and to improve the brooding behaviours of chicks in the first week of life. However, little information is available in this topic. Sixty 1-d old fayoumi chicks were reared in order to detect the behaviours and the levels of circulating IgY using ELISA technique. The resting beha...
The goal of the present study was to examine the suitability of a short pre-stimulation (P) for 15 s followed by a latency period (L) of 30 s before cluster attachment for machine milking. In addition we tested the effect of a periodic reduction of the vacuum under the teat (VR) during the massage phase from 43 kPa to 12-15 kPa on milking character...
Questions
Question (1)
I am Doing NOS2 staining for mouse brain tissue using goat Iba1 for microglia (novus) 1:500 and Rabbit anti-NOS2 (santa cruz) 1:500 for NOS2. For secondary Abs I used Donkey anti-goat and Donkey anti-Rabbit , while blocking was done by Normal Donkey serum 5% in BSA 2% for 2h. Staining of Iba1 is improved and clear enough , but I get high background and unclear staining for NOS2. Is there anybody has an experience with NOS2 Ab in Tissue immunofluorescence.
N.B: this Ab give me good data for western blot and cell culture immunoflurescence but it does not work for tissue. Thanks in advance