Mohammed Choukri

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Verified

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• Professor
• Pofessor at Mohamed I University

The verification of analytical performance is a critical step in ensuring the reliability and accuracy of laboratory assays. This study aims to evaluate the analytical performance of the beta-2 microglobulin (β2M) immunoassay on the Abbott Alinity ci® platform in the central laboratory of Mohammed VI University Hospital of Oujda over a 30-day durat...

Brain Natriuretic Peptide (BNP) is a good cardiac biomarker used for diagnostic, prognostic, and therapeutic monitoring in heart failure, but also in other affections like pulmonary arterial hypertension (PAH). The purpose of our work is to evaluate the analytical performance of the BNP assay on the Abbott Alinity ci® Analyzer at the Biochemistry l...

The verification of analytical methods, as required by the ISO 15189 standard, allows for the evaluation and comparison of a method’s performance against predefined analytical objectives. This study focuses on verifying the assay of Lipoprotein (a) on the Abbott® Alinity analyzer, contributing to accreditation and quality improvement in the laborat...

Leflunomide is an immunosuppressant indicated in the treatment of rheumatoid arthritis. This drug has a particular pharmacokinetics. Therapeutic Drug Monitoring (TDM) has been recommended for this drug because of its hepatic and hematological toxicities. We present here the prospective and retrospective analysis of a preliminary experience of the u...

In medical laboratories, the verification of methods ensures precise measurements, complying with regulatory standards like the Moroccan Guide for Medical Laboratory Analysis (GBEA) and ISO 15189. After organ transplantation, blood levels of cyclosporine are often monitored closely, and its dosage adjusted accordingly to maintain therapeutic levels...

The validation of analytical methods is mandated by the NF EN ISO 15189 standard. It consists of assessing an analytical method's performance based on a structured protocol and comparing the results to predefined analytical targets. In this context, this study evaluates the analytical performance of -reactive Protein (CRP) immunoassay using immunot...

The aim of our work was to evaluate the analytical performance of the Human Thyroid Stimulating Hormone (TSH) determination by a two-step immunoassay using microparticle chemiluminescence immunoassay (CMIA) technology, in accordance with the Scope A criteria of the guide of the verification/validation of medical biology methods. We evaluated the re...

Objective: The objective of our study was to characterize the sperm parameters of men consulting for infertility in our fertility center. Methods: The study included 3,025 patients investigated from September 2021 to July 2023. Demographic data, infertility risk factors, and the primary or secondary nature of infertility were recorded for each p...

The verification of analytical methods is a requirement outlined by the International Organization for Standardization (ISO). This process involves evaluating the performance of an analytical method according to a well-defined protocol and subsequently comparing it with pre-established analytical objectives. In our study, we conducted an evaluation...

This comprehensive paper amalgamates findings from three extensive studies conducted at Mohammed VI University Hospital Oujda, which examine the prevalence and predictors of vitamin D deficiency across different demographic groups: elderly patients, children, and postmenopausal women. The studies, covering a period of 92 months, retrospectively ana...

The verification of analytical methods is essential to ensure the accuracy of results. This study evaluates the performance of the transferrin assay method in the central laboratory of CHU Mohammed VI in Oujda, in order to confirm its reliability for the diagnosis and monitoring of iron metabolism disorders. We conducted a performance study of the...

The verification of analytical methods is crucial to ensure the accuracy of results. This study evaluates the performance of the microalbuminuria assay method in the central laboratory of CHU Mohammed VI in Oujda, to confirm its reliability for the diagnosis and monitoring of renal disorders and related conditions. We conducted a performance study...

The precision and reliability of the urine cannabinoids assay on the Abbott Alinity ci® analyzer were systematically evaluated. The study was conducted over 30 days in the biochemistry laboratory of Mohammed VI University Hospital. Two phases were carried out: reproducibility assessment involving daily measurements across low and high cannabinoid l...

The precision and reliability of the urine cannabinoids immunoassay on the Abbott Alinity ci® analyzer were systematically evaluated over 30 days in the biochemistry laboratory of Mohammed VI University Hospital. The study consisted of two phases: reproducibility assessment, involving daily measurements at low and high cannabinoid concentrations, a...

Background Anaemia remains the main cause of deferral in blood donation. However, in Morocco, pre‐donation haemoglobin measurement is not systematic. Objectives The aim of this study was to determine the prevalence of anaemia and associated factors such as sex, age and iron deficiency among blood donors in eastern Morocco in order to improve donor...

The combined use of Zingiber officinale (ginger) and Citrus limon (lemon) has long been valued in traditional medicine for their significant therapeutic properties, particularly in supporting cardiovascular health. However, their synergistic effects on hyperlipidemia and oxidative stress, both critical risk factors for cardiovascular diseases, rema...

The urinary creatinine assay is a pivotal diagnostic tool for assessing kidney function, hydration status, and muscle metabolism. This study evaluated the analytical performance of the urinary creatinine assay method using an Abbott kit on the Architect ci8200® automated system in the biochemistry laboratory of CHU Mohammed VI, Oujda. Method verifi...

This study aimed to evaluate the analytical performance of the serum phosphorus assay using the Abbott Architect ci8200® automated system in the biochemistry laboratory of CHU Mohammed VI, Oujda. Method verification was conducted following NF ISO 15189 standards and COFRAC guidelines, assessing key performance parameters such as repeatability, repr...

The primary objective of our study was to validate the analytical performance of complement C4 assay performed on the Abbott Alinity ci®: analyzer using the Immunoturbidimetric method. This validation was conducted in the biochemistry laboratory at Mohammed VI University Hospital in Oujda. The methodology followed the guidelines outlined in the fre...

Background: Argania spinosa seeds oil is an important endemic product of Morocco that is used traditionally as nephroprotective agent. Aim: Our study aims to compare the chemical compositions of unroasted (UnRoil) and roasted (Roil) Argania spi-nosa seed oils and their nephroprotective activity against gentamicin-induced kidney damage in Wistar rat...

Background and Aims: The precision of biological examination results hinges on meticulous preparatory measures before analysis, known as the pre-analytical phase. This phase contributes significantly to error rates (48-68%). Our study assessed the pre-analytical phase in seven hospital departments at Mohammed VI University Hospital. The objective w...

ASO (AntiStreptolysin O) is an antibody directed against streptolysin O which is an exotoxin secreted by Streptococcus pyogenes. The assay is mainly used for the diagnosis of post-streptococcal complications (AAR, nephropathy, etc.), False positives may be observed in cases of hyperlipemic serum, or liver disease. The objective of our work is to ev...

Background Monoclonal gammopathies (MG) are frequent, especially among older people. This study aims to establish the features and etiologies of MG detected over seven years in the Biochemistry department of Mohammed VI University Hospital in Morocco. Methods The study was performed from Jan 1, 2016, to Sept 1, 2023, and involved 224 patients resi...

The primary goal of our study was to verify the analytical performance of the 25-OH Vitamin D measurements conducted on the Abbott CI analyzer using the immuno-chemiluminescence method. This verification took place in the biochemistry laboratory at Mohammed VI University Hospital in Oujda. Our methodology was aligned with the recommendations outlin...

Background: COVID-19 is an infectious disease caused by the virus SARS COV 2 that has rapidly spread worldwide since December 2019, resulting in millions of severe cases and deaths. Elevated serum ferritin levels have been noted in patients with COVID 19. The objective of our study is to verify the prognostic role of serum ferritin in predicting th...

The primary goal of our study was to verify the analytical performance of intact parathyroid hormone measurements conducted on the Abbott CI analyzer using the immuno-chemiluminescence method. This verification took place in the biochemistry laboratory at Mohammed VI University Hospital in Oujda. Our methodology was aligned with the recommendations...

Multiple myeloma (MM) is a hematological malignancy marked by the abnormal proliferation of plasma cells that invade the bone marrow and, in most cases, secrete a monoclonal protein. While typically diagnosed in older individuals with an average age of 70, MM is rarely observed in young adults. Here, we present a unique case of MM in a 27-year-old...

The aim of our study was the verification of the analytical performance of Carcinoembryonic antigen determination on the Abbott CI analyzer utilizing the immuno-chemiluminescence method. The verification process was conducted in the biochemistry laboratory of Mohammed VI University Hospital of Oujda. The working methodology adapted is based on the...

Introduction: The aim of our study was to evaluate the analytical performance of the insulin dosage method, which was performed by the Alinity ci® automated system in the biochemistry laboratory of the Mohammed VI University Hospital in Oujda. Insulin acts as an essential role in regulating the body's energy supply, mainly by balancing blood sugar...

Introduction: Medicinal herbs used in traditional diabetes treatment are a rich source of anti-diabetic compounds. Pancreatic α-amylase inhibitors offer an effective strategy to reduce postprandial hyperglycemic levels via control of starch degradation. In this context, our study for the first time investigates the effect of Crocus sativus stamens...

The aim of our work was to evaluate the analytical performance of the anti-cyclic citrullinated peptide determination by a two-step immunoassay using microparticle chemiluminescence immunoassay (CMIA) technology, in accordance with the Scope A criteria of the guide of the verification/validation of medical biology methods. We evaluated the repeatab...

Magnesium is recognized as the fourth most important cation in the body and the second most abundant intracellular cation, following potassium. Its physiological significance lies in its capacity to form complexes with crucial intracellular anionic ligands, notably ATP, and its ability to compete with calcium for specific binding sites on proteins...

The verification of analytical methods is a requirement outlined by the International Organization for Standardization (ISO). This process involves evaluating the performance of an analytical method according to a well-defined protocol and subsequently comparing it with pre-established analytical objectives. In our study, we conducted an evaluation...

Objective: Recently, oral antioxidants in combined forms have been used to treat men with idiopathic infertility. This study aimed to evaluate the effects of treatment with vitamin C, vitamin E, selenium, zinc, arginine, L-carnitine, and coenzyme Q10 on sperm quality parameters, DNA integrity, reproductive hormones, and pregnancy rates in men with...

Background DNA fragmentation index (DFI) enhances routine semen analysis by providing valuable insights into male reproductive potential. Utilizing Halosperm test, a sperm chromatin dispersion (SCD) assay based on induced condensation. The purpose of this study was to assess sperm DNA damage both before and after freezing. By following the specifie...

The aim of our work was to evaluate the analytical performance of CA 125 determination by a two-step immunoassay using microparticle chemiluminescence immunoassay (CMIA) technology, in accordance with the Scope A criteria of the guide of the verification/validation of medical biology methods. We evaluated the repeatability and the intermediate prec...

Background The precision of test measurements is critical in clinical diagnostics, especially for biomarkers like total PSA and homocysteine, which are essential to disease assessment. Using the CMIA approach, this study investigates the repeatability and reproducibility of these biomarkers on the Abbott Alinity system. Methods The present study w...

Introduction: The aim of our study is to evaluate the haptoglobin assay on the Alinity ci automated system. This evaluation is part of an overall approach to verifying the methods used in the central laboratory of the Mohammed VI University Hospital of Oujda , with a view to compiling an accreditation file in accordance with the requirements of sta...

The aim of our study was the verification of the analytical performance of alpha-fetoprotein determination on the Abbott CI analyzer utilizing the immuno-chemiluminescence method. The verification process was conducted in the biochemistry laboratory of Mohammed VI University Hospital of Oujda. The working methodology adapted is based on the recomme...

Introduction: CA19-9 is a marker used in the diagnosis and monitoring of several cancer diseases. The aim of this study is to evaluate the CA19-9 assay on the Abbott ALinity Ci automated system. This evaluation is part of an overall approach to verifying the methods used in the CHU's central laboratory, with a view to compiling an accreditation fil...

Alzheimer’s disease (AD) is the most prevalent type of dementia. Pathologically, the disease is marked by neurofibrillary tangles (NFT), which are aberrant accumulations of the tau protein that develop inside neurons, and extracellular plaque deposits of the amyloid β peptide (Aβ). These pathological lesions are present in the brain before the begi...

The pandemic of coronavirus disease (Covid-19) has infected millions of individuals around the globe. The current study was aimed to evaluate the serum procalcitonin (PCT) level as a biomarker for bacterial co-infection and disease severity in Covid-19 patients. A total of 319 Covid-19 positive patients were examined in this study. Among the total...

The standard NF EN ISO 15 189 mandates the verification of analytical methods. It involves evaluating an analytical method's effectiveness in accordance with a precisely laid out protocol before comparing it to previously established analytical objectives. Any biologist must be concerned with mastering this approach. In this work, we compare two au...

The precision and reliability of the serum glucose assay on the Abbott Alinity ci® analyzer were systematically evaluated. The study was conducted over 30 days in the biochemistry laboratory of Mohammed VI University Hospital. Two phases were carried out: reproducibility assessment involving daily measurements across low, medium, and high glucose l...

Introduction: As part of our study, we set out to evaluate the analytical performance of the gamma-glutamyl transferase (GGT) assay using an Abbott kit on the Alinity ci® automated system in the biochemistry laboratory of the CHU Mohammed VI in Oujda. Materials and methods: This study assessed the repeatability and reproducibility of Alinity ci® an...

Serum total cholesterol measurement is an essential aspect of preventive healthcare as high levels of cholesterol are linked to various diseases such as atherosclerosis, heart attacks, and strokes. Serum cholesterol measurement is a simple blood test that assesses the levels of cholesterol in the bloodstream. It is an important diagnostic tool that...

In addition to using the most up-to-date analytical technique, a thorough preparation phase must come first in order for the biological examination results to be reliable. The pre-analytical phase is this important step. We decided to conduct a prospective and descriptive study within central Laboratory department of the Mohammed VI University Hosp...

Introduction azoospermia affects more than 10%-15% of infertile male subjects attending the infertility center. In Morocco, there have been no studies on male infertility with azoospermia. Thereby, our objective was to evaluate the clinical, hormonal, and genetic characteristics of infertile men with azoospermia in Morocco. Methods we conducted a...

Medical samples (tissues, cells) and accompanying documents require bar-coding to be able to identify the donor of the tissue or cells collected, the location, and the date and time of collection. The time at which the sample is taken this time sets the starting point for the storage time. This paper proposes an application of the Internet of Thing...

The verification of analytical methods is a requirement of the standard NF EN ISO 15 189. It consists of evaluating the performance of an analytical method according to a well-defined protocol and then comparing it with pre-established analytical objectives. The mastery of this approach must be the concern of any biologist. Through this work we pre...

Introduction With the spread of the Covid-19 pandemic and its overwhelming impact on health systems in several countries, the importance of identifying predictors of severity is of paramount importance. The objective of this study is to determine the relationship between death and the biological parameters of patients with Covid-19. Materials and m...

Carbon tetrachloride (CCl4) is one of the commonly used models for the induction of liver injury and screening of the hepatoprotective activity of different drugs. The aim of this study was to investigate the hepatoprotective activity and possible underlying mechanisms of methanolic and hydroethanolic extracts of Crocus sativus stamens using a mode...

Background Coronavirus disease (COVID-19), caused by a betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has rapidly evolved into a pandemic since it was first reported in December 2019. thus, SARS-CoV-2 has become a major global public health issue. Objective The objective of this work is to compare demographics, como...

The most costly spice in the world, Crocus sativus L. (C. sativus), has been used for more than 3,000 years. It has various bene cial applications in a range of elds, including aromas, colorants, and medications, but its usefulness as a food avoring and coloring ingredient is the highest. Large quantities of by-products from the processing of sa ro...

The objective of this study was to independently evaluate the analytical performance of the albumin assay on the Abbott Alinity ci automated system and to ensure the best reliability of the results according to the recommendations of the NF ISO 15,189 standard. Our study was performed according to the Scope A criteria detailed in the Medical Biolog...

Thymelaea microphylla Coss. et Dur (T. microphylla), a medicinal herb that grows in arid and desert pastures, has been traditionally utilized by Moroccans to treat many diseases, including kidney failure. This study aims to evaluate the nephroprotective effect against gentamicin-induced nephrotoxicity (GM), and thus the antioxidant activity of an a...

The preanalytical phase of medical biology examinations is often not mastered by the laboratory staff, and represents an important source of non-conformities of the analyses, which can affect the quality of the results of the examinations. The objective of our work is the presentation of a devise model for the management of information concerning b...

The laboratory's activities lead to the storage of chemicals, analysis kits, biological samples, single-use material, etc. Depending on the storage conditions of certain products, it is necessary to create storage areas at different temperatures. The objective of our work is to underline the importance of the cold chain in the medical biology labor...

Water is the most used reagent in clinical laboratories. It is used for all levels of chemical, biological and physical analysis. It is important to know the type of water used because many analyses can be affected by its quality. Several agencies have established preparation and purity criteria for water. The objective of our work is to highlight...

The objective of our work is the evaluation of analytical performance by verifying the method of determination of serum aminotransferases by an Abbott kit on two Architect ci8200 automatons in the biochemistry laboratory of Mohammed VI University Hospital of Oujda. The verification of the analytical performance of the aminotransferase assay kits is...

COVID-19 was discovered in China for the first time in December 2019 and was declared a pandemic by the World Health Organization on March 11, 2020. Due to its rapid geographic expansion over the last three years, it has now become a global health issue. The infection is caused by SARS-CoV-2, which is obtained from a zoonotic source and transmitted...

COVID-19 is an emerging viral disease with incompletely elucidated pathogenesis, a heterogeneous clinical profile, and significant interindividual variability. The major cardiovascular complications of COVID-19 include acute cardiac injury, acute myocardial infarction (AMI), myocarditis, arrhythmia, heart failure, and venous thromboembolism (VTE)/p...

Vitamin D is one of the steroid hormones. Approximately 80% of vitamin D comes from skin production under the effect of sunlight. A small portion of vitamin D also comes from food and/or dietary supplements. Vitamin D is well known for its role in phosphocalcic metabolism and bone mineralization. Several studies suggest that vitamin D has reproduct...

The objective of our study was to determine the vitamin D status in the population of Eastern Morocco, and to study vitamin D2 and vitamin D3 by high performance liquid chromatography. The mean total vitamin D level was 18.66 ng/ml. 21.66 ng/ml for men and 16.78 ng/mL for women. The results indicate the fact that even in a region such as ours, whic...

The purpose of this work is to present the results of a comparative study between the 25-Hydroxyvitamin D [25 (OH) vitamin D] assay on Architect ci8200® (Abbott) and Cobas e411® (Roche Diagnostics). 100 samples of venous blood were randomly selected from routine vitamin D tests. The assay for 25 (OH) vitamin D was performed on Architect ci8200® (Ab...

The objective of this study is the verification of a portable device for the traceability and the management of information concerning blood samples in real time, developed in the biochemistry laboratory of CHU Mohammed VI of Oujda. This device allows the traceability of : Date and exact time of the sampling, identity of the patient, identity of th...