Mohammed Al-zubaidi

• Doctor of Philosophy
• Assistant Professor at Mustansiriyah University

Human induced pluripotent stem cells (hiPSCs) offer an exceptional opportunity for generating disease-specific models to study and explore the underlying mechanisms. The present study aims to perform bioinformatics analysis to explore the potential hub genes, cluster as well as functional pathways depended upon the differentiation of induced plurip...

Human pluripotent stem cells (hPSCs) have widespread potential biomedical applications. There is a need for large-scale in vitro production of hPSCs, and optimal culture methods are vital in achieving this. Physiological oxygen (2% O2) improves key hPSCs attributes, including genomic integrity, viability, and clonogenicity, however, its impact on h...

The biochemical diagnostic test is important in the identification of patients with polycystic ovary syndrome (PCOS). Fourier Transfer Infrared (FTIR) measurement which analyzes the overall metabolic profiling of serum may provide more biochemical information. Here we applied the FTIR spectroscopy with multivariate analysis for a PCOS diagnosis. Si...

Biochemical tests are critical in the management of patients with hypothyroidism (HT). Fourier Transfer Infrared (FTIR) which allows for global bio-fluid metabolic profiling may give more appropriate information by measuring a broad range of metabolic parameters in bio-fluid. Here we present the application of FTIR with chemo metric analysis for th...

This chapter will provide a concise overview of the history of mammalian in vitro cell culture from its first descriptions through to the modern era. A specific focus will be adopted to understand when the importance of oxygen control emerged within the field, and we will discuss some of the earliest examples. A description of the common and state-...

Zinc, Copper, Selenium, Magnesium, Manganese, Chromium, Iron, Nickel, Cobalt, Vanadium and Germanium were determined by atomic absorption spectrophotometer (AAS) in blood serum of patients with rheumatoid arthritis, (30) patients (14male and 16female) with age range (37-60) years compared with normal tensive control. The analysis of results showed...

MicroRNAs are reported to have a crucial role in the regulation of self-renewal and differentiation of stem cells. Hypoxia has been identified as a key biophysical element of the stem cell culture milieu however, the link between hypoxia and miRNA expression in stem cells remains poorly understood. We therefore explored miRNA expression in hypoxic...

qRT-PCR data showing inverse correlation of expression of (a) down-regulated and (b) up-regulated predicted target genes of HRMs in hESCs and similarly for hMSCs (c) downregulated and (d) upregulated. Graphical data points in a-d represent mean ± S.D. of a minimum of three independent experiments. (*P>0.01 and <0.05, **P<0.01). (TIF)

Quantitative RT-PCR data of (a) up-regulated and (b) down-regulated HRMs in a different human bone-marrow derived MSCs. QRT-PCR data showing levels of Drosha and DICER in hMSCs grown in normoxia and hypoxia (c). Graphical data points in a, b and c represent mean ± S.D. of a minimum of three independent experiments. (*P>0.01 and <0.05, **P<0.01). (T...

Pathway analysis of hypoxia regulated miRNAs. A figure showing miRNA:target gene interaction network for HIF-1 signalling pathway in hESCs drawn using Cytoscape software. The green color refers up-regulation while the red color refers down-regulation. (TIF)

qRT-PCR data of (a) up-regulated and (b) down-regulated HRMs in SHEF2. QRT-PCR data showing levels of Drosha and DICER in hESCs grown in normoxia and hypoxia (c). Graphical data points in a, b and c represent mean ± S.D. of a minimum of three independent experiments. (*P>0.01 and <0.05, **P<0.01). (TIF)

Pathway analysis of hypoxia regulated miRNAs. A figure showing miRNA: target gene interaction network for pathways in cancer of hESCs (a) and hMSCs (b) drawn using Cytoscape software. The green color refers up-regulation while the red color refers down-regulation. (TIF)

List of upregulated (Sheet 1 and 3) and downregulated (Sheet 2 and 4) miRNAs showing >2 fold difference in response to hypoxia in SHEF1 cells and human bone marrow derived MSCs based on microarray expression profiling data. (XLSX)

List of predicted targets of up-regulated and down-regulated miRNAs in hE & MSCs using DIANA-MicroT-CDS program. (XLSX)

List of analyzed pathways of hypoxia up-regulated (8a, b) and down-regulated (8c, d) miRNAs in hE & MSCs, respectively, using DIANA-mirPath program. (XLSX)

List of primers used for the detection of specific miRNAs using Quantitative RT-PCR. (DOCX)

List showing location of HREs and their scores in the promoters of hypoxia induced miRNAs based on python program. (XLSX)

Objective: To study the trace element levels and the effect of methylprednisolone and interferon beta-1b on these levels in the serum of patients with multiple sclerosis. Methods: This study was conducted on 32 patients who received methylprednisolone, 32 patients who received interferon beta-1b and on 32 patients who received no treatment at the M...

Background: A potentially important role for calcium in the development of diabetes has been implicated. This study aims to the assess serum calcium (Ca) and parathyroid hormone (PTH) levels in diabetic patients and the correlation between calcium and PTH with the duration of the disease and age of the patients. Material & Method: This study includ...