Mohammad Zahangir Alam

Bangladesh Agricultural University | BAU · Department of Parasitology

Gastrointestinal parasitism and anthelmintic efficacy are of global concern for livestock and public health. An investigation on cattle gastrointestinal parasites and anthelmintic efficacy was conducted at Fulbaria Upazila, Mymensingh in 2017. Out of 100 fecal samples examined using simple sedimentation method, 70% cattle were found positive for pa...

Haemonchus contortus is the most prevalent and pathogenic gastrointestinal nematodes (GINs) in ruminants causing extensive economic losses. It is essential to estimate the efficacy of common commercially available anthelmintics against Hae-monchus contortus parasite. Here, we standardized an ex-vivo culture platform for H. contortus and evaluated t...

Anthelmintic resistance against gastrointestinal nematodes especially H. contortus of sheep and goat is a global issue. To address the gravity and extension of AR in Bangladesh, genotyping of 160 adult H. contortus parasites were performed to confirm benzimidazole resistance allele from different geographic zones of Bangladesh based on allele speci...

Cysticercus tenuicollis is a larval stage of Taenia hydatigena resulting in cysticercosis, and responsible for enormous economic loses, especially in livestock production. Here, we planned to determine the prevalence and explore genetic variation of C. tenuicollis isolated from goats based on small subunit ribosomal RNA (rrnS) and cytochrome oxidas...

Fasciola gigantica, the causative agent of tropical fasciolosis, is a food-borne zoonotic trematode that affects around 80% livestock of Bangladesh. Triclabendazole (TCBZ), nitroxynil (NTON) and oxyclozanide (OCZN) are frequently used against fascioliasis; however, the current status of potency of these flukicides was unknown. In this study, in vit...

Background: Eimeria tenella is the most pathogenic intracellular protozoan parasite of seven Eimeria species causing chicken coccidiosis around the world. This species is particularly responsible for caecal coccidiosis leading to serious morbidity-mortality and financial loss in poultry production. Methods: The present study explored the genetic...

Echinostomes (Trematoda: Echinostomatidae) are food-borne zoonotic flatworms that affect birds, animals and humans, and has been classified as neglected tropical diseases (NTDs) by the World Health Organization (WHO), which cause severe enteritis in poultry and hamper production. Here, we confirmed the species of echinostomes affecting chickens in...

Abstract Background: The important trematode species in small ruminants: Paramphistomum sp., Fasciola spp. and Schistosoma spp. seriously affect the productivity of domestic ruminants in endemic areas. Methods: In the present study, we identified the potential risk factors associated with trematodes infections in small ruminants in seven topographi...

Objectives: The purpose of this study was to determine the species composition of Eimeria circulating in Mymensingh district, Bangladesh, using Internal Transcribed Spacer 1 (ITS1) sequences in polymerase chain reaction (PCR) assay. Materials and Methods: Coccidian oocysts were isolated and sporulated in a solution containing 2% potassium dichromat...

Objectives: The purpose of this study was to determine the species composition of Eimeria circu¬lating in Mymensingh district, Bangladesh, using Internal Transcribed Spacer 1 (ITS1) sequences in polymerase chain reaction (PCR) assay. Materials and Methods: Coccidian oocysts were isolated and sporulated in a solution containing 2% potassium dichrom...

Gastrointestinal (GI) parasites are major contributors to decrease productivity in livestock over the world. A cross-sectional study was conducted in different areas of Bangladesh to determine the prevalence of GI parasitic infections and their association with the biotic and abiotic factors in sheep. A total of 572 faecal samples were collected fr...

Farmers’ knowledge and farm management practices can potentially help to mitigate the prevalence and severity of chicken coccidiosis, a devastating protozoan disease. Here, we assessed the farmers’ perception and on-farm practices of coccidiosis management in small-scale broiler farms at Gazipur district, Bangladesh. A semi-structured questionnaire...

Gastrointestinal nematode infections of livestock are ranked in the top twenty diseases affecting small-holder farmers’ livestock. Mecistocirrus digitatus is one of the most prevalent parasitic nematode among the trichostrongylids causing severe health hazards leading to production losses in cattle worldwide. This study was conducted to explore the...

Coccidiosis is one of the major economically important diseases of poultry industry worldwide. In Bangladesh, coccidiosis is one of the major threats for commercial poultry industry as well. Successful control of chicken coccidiosis in Bangladesh mainly depends on the application of anticoccidial drugs (ACD) in a poultry house during grow-out. Ther...

Background: Ecto-parasites namely ticks, lice and flea are important due to their blood sucking habit, skin damage and acting as vectors for various disease pathogens in both livestock and humans. The aims of this study were to estimate the prevalence and identify potential factors associated with ecto-parasitic infestations at Mymensingh Sadar, My...

Goats greatly influence the economic sustainability of rural communities. However, parasitic diseases, especially gastrointestinal nematodes (GINs) are a major constraint on profitable small ruminants' production worldwide. During July- 2015 to June- 2016, we conducted a cross sectional study within seven topographic zones of Bangladesh to explore...

Protozoan parasites of the Leishmania donovani complex – L. donovani and L. infantum – cause the fatal disease visceral leishmaniasis. We present the first comprehensive genome-wide global study, with 151 cultured field isolates representing most of the geographical distribution. L. donovani isolates separated into five groups that largely coincide...

Protozoan parasites of the Leishmania donovani complex – L. donovani and L. infantum – cause the fatal disease visceral leishmaniasis. We present the first comprehensive genome-wide global study, with 151 cultured field isolates representing most of the geographical distribution. L. donovani isolates separated into five groups that largely coincide...

Protozoan parasites of the Leishmania donovani complex – L. donovani and L. infantum – cause the fatal disease visceral leishmaniasis. We present the first comprehensive genome-wide global study, with 151 cultured field isolates representing most of the geographical distribution. L. donovani isolates separated into five groups that largely coincide...

Anthelmintic resistance (AR) against gastrointestinal nematodes (GINs) of sheep and goats is a global concern. To address the problem, this study assessed the status of AR in different government and private sheep and goat farms in Bangladesh. We conducted fecal egg count reduction test (FECRT) and Egg hatch assay (EHA) experiments. For the detecti...

Endoparasitism is among the important causes of reduced productivity of chickens. To investigate the prevalence of intestinal parasitic diseases, a study was carried out from March, 2017 to February, 2018 in dead broiler and sonali chickens from different commercial farms in Sirajgonj district by postmortem examination. A total of 500 intestinal sa...

Fish-borne liver and intestinal flukes are helminth pathogens that have a negative impact on public health worldwide. We herein investigated the status of infection by the metacercariae (MC) of fish-borne trematodes (FBTs) in randomly selected freshwater wild fishes. Five species of fishes were collected and digested artificially using digestion fl...

The study was planned to apply the PCR method for detection of T. gondii infection in sheep, goats and cattle aborted fetuses from Mymensingh, Bangladesh. A total of 58 fetal tissue samples (brain, liver, heart, skeletal muscle and placenta) of sheep (5), goats (5) and cattle (2) were selected for study. Aborted fetuses were taken from serologicall...

Protozoan parasites of the Leishmania donovani complex, L. donovani and L. infantum, cause the fatal disease visceral leishmaniasis. We present the first comprehensive genome-wide global study, with 151 cultured field isolates representing most of the geographical distribution. L. donovani isolates separated into five groups that largely coincide w...

Haemonchus contortus is the most prevalent parasitic nematode among the Trichostrongylids causing severe health hazards leading to production losses in small ruminants around the world. This study was conducted to explore genetic variation within and among H. contortus populations from seven topographic zones of Bangladesh in small ruminants using...

Anthelmintic resistance has become a global problem that threatens livestock production worldwide. The present study was investigated the status of anthelmintic resistance in gastrointestinal (GI) nematodes of small ruminants in two organized sheep and goat farms in two different areas of Bangladesh by fecal egg count reduction test (FECRT) for alb...

Linguatula serrata, a food-borne zoonotic pentastomid that affects a wide range of animals including humans. Here, the study was conducted on some epidemiologic and pathologic aspects of linguatulosis in cattle and goats through a slaughterhouse based survey. A total of 302 samples, consisting of 257 mesenteric, 26 hepatic and 19 pulmonary lymph no...

Haemosporidian parasites of the genera Plasmodium and Haemoproteus can cause declined productivity and high mortality in pigeons and other domestic and wild birds. This study was carried out to investigate the status of pigeon haemosporidian parasites in two northern districts (Mymensingh and Rangpur), Bangladesh. In total, 300 blood samples of pig...

Visceral leishmaniasis (VL), an important tropical disease, is caused by the intracellular protozoan parasites of the Leishmania donovani complex. VL is a serious public health problem in the Indian subcontinent, and there are 40,000-45,000 cases per year in Bangladesh 1. VL, caused by L. infantum (synonym L. chagasi) is a zoo-notic disease, and is...

Food-borne parasitic zoonoses are major threats to human health and only next to the ‘big three’ such as AIDS, tuberculosis and malaria. Linguatula serrata is a food-borne zoonotic pentastomid that affects a wide range of animals including humans. Dogs and other carnivores are the final hosts while most herbivores, including domestic ruminants serv...

Parasitic infections are common in stray dogs and accurate knowledge of parasite communities in dogs would provide insight into the epidemiology of parasitic diseases. In this study, we used Illumina sequencing technology to evaluate cell-free DNA (cfDNA) as a marker for screening of parasitic infections in dogs. Plasma samples from 14 stray dogs c...

Anthelmintic resistance (AR) to commonly used dewormers is one of the major world-wide constrain in livestock production. The present study was investigated the status of AR in BAU dairy farm, Mymensingh and Talukder dairy farm, Sirajganj. Faecal egg count reduction test (FECRT) was applied to assess AR in cattle of two dairy farms during January t...

The present epidemiological investigation was carried out to investigate the status of pigeon haemosporidian parasites in two districts (Mymensingh and Rangpur), Bangladesh. In total, 300 blood samples of pigeons were examined from January to November, 2016. Blood samples were collected from wing vein and thin smears were prepared from them. All sm...

p>Avian haematozoans are important pathogens of birds causing asymptomatic to fatal infections. The present study was undertaken to investigate the prevalence and associated risk factors of malaria parasite infections in domestic chickens and ducks in three localities namely Mymensingh, Tangail and Netrokona districts in Bangladesh. In total, 474 b...

p>Gastrointestinal (GI) parasitism is anegregious problem in small ruminant production system due to is impact on growth and associated cost of control measures. An epidemiological study was conducted to investigate the prevalence of gastrointestinal parasites of small ruminants from different areas of Madhupur, Tangail. The stool samples were coll...

Objective: The current study was conducted to explore the prevalence of gastrointestinal (GI) parasites in small ruminants (sheep and goats) in relation to species, age, sex, season, and body condition in Mymensingh, Bangladesh. Materials and methods: A cross-sectional coprological survey was carried out at Mymensingh Sadar in Mymensingh from July...

To estimate the seroprevalence of toxoplasmosis and risk factors for seropositivity in sheep, goats and cattle in Dhaka, Mymensingh, Sirajganj and Chittagong districts in Bangladesh, 1104 sera samples (552 sheep, 300 goats and 252 cattle) were randomly selected and tested by an indirect enzyme linked immunosorbent assay (iELISA). The overall seropr...

Toxoplasmosis is a great zoonotic threat that affects human species specially women. It has got major health significance because it causes abortion in pregnant women. The aim of this study was to determine seroprevalence of toxoplasmosis in women in Mymensingh and Rangpur in Bangladesh and to identify possible risk factors associated with toxoplas...

Toxoplasma gondii is a protozoan parasite which can infect virtually all warm blooded animals including humans. The present study was conducted to determine the seroprevalence of T. gondii infection in goats (Capra hircus) and risk factors as well. A total of 159 caprine sera samples collected randomly from Inaruwa municipality and surrounding area...

p>This study was undertaken to investigate the effect of sulphaclozine sodium and toltrazuril for the treatment of experimentally induced caecal coccidosis. Day-old chicks (n=96) reared in a coccidia free condition divided into six Groups (A, B, C, D, E and F) each having 4 subgroups of 4 chicks in each. At the age of 23 days the chicks were infect...

Visceral leishmaniasis (VL), or kala-azar, is mainly caused by two closely related Leishmania species, Leishmania infantum and Leishmania donovani Leishmania infantum is responsible for zoonotic VL, with dogs as the main reservoir host in the Mediterranean, the Middle East, Asia, and South America. In the Indian subcontinent, VL is caused by L. don...

Emerging tick-borne diseases (TBDs) are important foci for human and animal health worldwide. However, these diseases are sometimes over looked, especially in countries with limited resources to perform molecular-based surveys. The aim of this study was to detect and characterize spotted fever group (SFG) rickettsiae and Anaplasmataceae in Banglade...

Phylogenetic analysis of Anaplasma spp. detected in this study based on partial sequences of 16S rDNA using a maximum likelihood method.

Pulmonary parasitic infections in goats are of considerable economic importance. The parasites cause chronic production losses as a result of reduced food conversion ratio (FCR) and weight gain. To investigate the prevalence of pulmonary parasites of goat, a total of 45 lungs were examined from Mymensingh Sadar during the period January to May, 201...

Babesia gibsoni is a tick-borne hemoprotozoan parasite of dogs that often causes fever and hemolytic illness. Detection of B. gibsoni has been predominantly reported in Asian countries, including Japan, Korea, Taiwan, Malaysia, Bangladesh and India. The present study shows the first molecular characterization of B. gibsoni detected from dogs in Ban...

In Morocco, cutaneous leishmaniasis (CL) caused by Leishmania (L.) tropica is a major public health threat. Strains of this species have been shown to display considerable serological, biochemical, molecular biological and genetic heterogeneity; and Multilocus Enzyme Electrophoresis (MLEE), has shown that in many countries including Morocco heterog...

Background Cutaneous leishmaniasis (CL) is a major and fast increasing public health problem, both among the local Pakistani populations and the Afghan refugees in camps. Leishmania (Leishmania) major is one of the etiological agents responsible for CL in Pakistan. Genetic variability and population structure have been investigated for 66 DNA sampl...

The Leishmania strains from different epidemic areas in China were assessed for their genetic relationship. Twenty-nine strains of L. infantum isolated from 1950 to 2001 were subjected to multilocus microsatellite typing (MLMT) using 14 highly polymorphic microsatellite markers. Twenty-two MLMT profiles were recognized among the 29 L. infantum stra...

Abstract: The present study was undertaken in different areas of Tangail district to investigate the presence of haemosporidian parasites in domestic poultry (Chicken, duck and pigeon). A total of 72 samples were examined from December, 2013 to May, 2014. Microscopical examinations of peripheral blood were performed. Among the examined samples, 33(...

Different species of vector snails carry larval stages of different trematodes. Studies were performed to investigate different snail species and parthinate of trematodes carried by them, in some selected areas of Mymensingh Sadar, Mymensingh. Snails were collected by hand picking method and examined after crushing. Among 864 tested snails, it reve...

To study the effect of months, seasons, age and sex on visceral leishmaniasis (VL) in human, data were collected during March 2010 through February 2011 from the register book of Upazila Health Complex (UHC) of Trishal, which is an endemic region for VL in the Mymensingh district. Besides, 70 blood samples were collected from suspected VL human pat...

Although Phlebotomus argentipes as the only known vector of visceral leishmaniasis (VL) is zoophilic in nature, VL is considered to be anthroponotic in the Indian subcontinent. Peripheral blood samples from 85 stray dogs were examined for any molecular evidence of Leishmania infection in VL endemic areas of Bangladesh. Parasite DNA was detected in...

Cutaneous leishmaniasis (CL) is a major public health problem in Libya. The objective of this study was to investigate, for the first time, epidemiological features of CL outbreaks in Libya including molecular identification of parasites, the geographical distribution of cases and possible scenarios of parasite transmission. We studied 450 patients...

Cutaneous leishmaniasis (CL) in Morocco is caused by three species, Leishmania major, L. tropica and L. infantum. CL has been known in Chichaoua province since 2000. Using DNA extracted from microscopic slides and parasite cultures, collected in the years 2006 and 2009, we identified for the first time L. tropica as the causative agent of CL in thi...

Different species of vector snails carry larval stages of different trematodes. Studies were performed to investigate different snail species and parthinate of trematodes carried by them, in some selected areas of Mymensingh Sadar, Mymensingh. Snails were collected by hand picking method and examined after crushing. Among 864 tested snails, it reve...

bT – Reference strain of the species; 1 – zymdemes according to the Montpellier system – MON [86] or the CLIOC system – IOC/Z [44], MON-1 and Z1 are corresponding zymodemes; 2 – Population assignment according to STRUCTURE analysis of the combined dataset of 409 strains of NW and OW L. infantum; 3 – Population assignment according to STRUCTURE anal...

Neighbor-joining tree of NW L. infantum genotypes. This midpoint-rooted tree was derived from the Chord-distance calculated for the MLMT profiles of 14 microsatellite markers. Only different genotypes are scored. Clinical pictures, reservoir, origin and number of strains sharing the same genotype are indicated, as well as population and sub-populat...

Neighbor-joining tree showing only population 1 of the combined NW and OW strains of L. infantum. This unrooted tree is based on the Chord-distance of the MLMT profiles of 14 microsatellite markers and it includes only strains belonging to population1 (MON-1) of the combined OW (dots) and NW (squares) L. infantum data set (see Fig. 5 and 6). Isolat...

N, number of strains; P, proportion of polymorphic loci; MNA, mean number of alleles; Ho, observed heterozygosity; He, expected heterozygosity; FIS, inbreeding coefficient; FR – France; IT – Italy. (DOC)

Additional information about the methods of MLMT data analyses. (DOC)

Midpoint-rooted neighbor-joining tree showing all individual NW L. infantum strains. Phylogenetic tree derived from the Chord-distance calculated for the MLMT profiles of 14 microsatellite markers for the 98 NW strains of L.infantum studied. Clinical pictures and reservoir for each strain are indicated. The origin of the Brazilian strains (state) i...

Leishmania infantum (syn. L. chagasi) is the causative agent of visceral leishmaniasis (VL) in the New World (NW) with endemic regions extending from southern USA to northern Argentina. The two hypotheses about the origin of VL in the NW suggest (1) recent importation of L. infantum from the Old World (OW), or (2) an indigenous origin and a distinc...

The Namangan Region in the Pap District, located in Eastern Uzbekistan is the main focus of visceral leishmaniasis (VL) in Uzbekistan. In total, 28 cases of human VL were registered during 2006-2008 in this region. A study on the epidemiology of VL in this area was carried out in 2007-2008 in the villages of Chodak, Oltinkan, Gulistan and Chorkesar...

We assessed the prevalence and seasonal variations of intestinal schistosomiasis in association with age, sex, breed and rearing system of cattle and goats in different districts of Bangladesh. Fecal samples from 240 cattle and 146 goats were examined. Schistosoma eggs were found in 47.5% cattle and 43.84% goats. Two species of schistosomes were id...

We assessed the prevalence and seasonal variations of intestinal schistosomiasis in association with age, sex, breed and rearing system of cattle and goats in different districts of Bangladesh. Fecal samples from 240 cattle and 146 goats were examined. Schistosoma eggs were found in 47.5% cattle and 43.84% goats. Two species of schistosomes were id...

Our present study is the first attempt to characterize Leishmania parasites from foci in Uzbekistan and Tajikistan endemic for visceral leishmaniasis (VL). PCR-sequencing of the ribosomal internal transcribed spacer 1 and multilocus microsatellite typing (MLMT) were applied to DNA extracted from preparations of Giemsa-stained bone marrow aspirates...