Mohammad Vatanparast

Mohammad Vatanparast
Johannes Gutenberg-Universität Mainz | JGU · Institut of Organismic and Molecular Evolution

PhD

About

37
Publications
31,196
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229
Citations

Publications

Publications (37)
Article
Full-text available
The red imported fire ant (RIFA), Solenopsis invicta Buren is native to South America and is known as a global problematic invasive species. This study focused on the molecular response of RIFA by comparing gene expression profiles after exposing ants to low (10 °C) and high (40 °C) temperature stress and comparing them to untreated controls (30 °C...
Article
The fall armyworm, Spodoptera frugiperda, native to the tropical and subtropical areas of the American continent is one of the world's most destructive insect pests. In most insects, sex pheromone production is initiated following the activation of a pheromone-biosynthesis-activating neuropeptide (PBAN) receptor, which belongs to G protein-coupled...
Article
Full-text available
The fall armyworm (FAW), Spodoptera frugiperda, is native to the tropical and subtropical areas of the American continent and is one of the world's most destructive insect pests and invaded Africa and spread to most of Asia in two years. Glycerol is generally used as a cryoprotectant for overwintering insects in cold areas. In many studies, the inc...
Article
The red imported fire ant (RIFA), Solenopsis invicta, is one of the globalized invasive pests. This study focused on pathogenicity and virulence of entomopathogenic fungi as one of the biological control agents to RIFA workers under different temperatures. The fungal pathogen, Beauveria bassiana ANU1 was isolated from Korea in 2015 and showed the p...
Article
Full-text available
The fall armyworm (FAW), Spodoptera frugiperda, is regarded as one of the world's most harmful plant pests. This research examines the molecular response processes of FAW to low temperature (4 • C) and high temperature (40 • C) when gene expression is compared to controls (25 • C). A total of 211,967 unigenes were collected, at least 14,338 of whic...
Article
The red imported fire ant (RIFA), Solenopsis invicta Buren is native to South America and known as a global problematic invasive species. At low temperatures, several investigations have demonstrated an increase in glycerol as a primary rapid cold hardening (RCH) component and an increase in the supercooling point. Two genes, glycerol-3-phosphate d...
Article
Full-text available
Solenopsis japonica, as a fire ant species, shows some predatory behavior towards earthworms and woodlice, and preys on the larvae of other ant species by tunneling into a neighboring colony's brood chamber. This study focused on the molecular response process and gene expression profiles of S. japonica to low (9 °C)-temperature stress in compariso...
Article
Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae), the cotton bollworm, is a destructive pest which is famous for its resistance to a variety of insecticides. RNA interference is a posttranscriptional gene silencing mechanism that has become a popular tool to control insect pests, triggered by double-stranded RNAs (dsRNAs). The effect of inges...
Article
Protection from parasitism by a virus Parasitoid wasps have developed myriad systems to overcome the defense mechanisms of their hosts as they lay their eggs in the bodies and eggs of targeted species. Gasmi et al . report how the host can fight back when infected by a virus that expresses a protein conferring resistance to the parasitoid. When mem...
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Repat (=response to pathogen) is proposed for an immune-associated gene family from Spodoptera exigua, a lepidopteran insect. In this gene family, 46 members (Repat1–Repat46) have been identified. They show marked variations in their inducible expression patterns in response to infections by different microbial pathogens. However, their physiologic...
Article
Solenopsis japonica, which is belonging to Formicidae in Hymenoptera, is a native ant species in Korea. However, it had not been studied for cold hardiness of S. japonica to understand on its overwintering mechanisms in field so far. Cold tolerance on developmental stages was measured at different cold temperature with various exposure times. Worke...
Article
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Epoxyoctadecamonoenoic acids (EpOMEs) are epoxide derivatives of linoleic acid (9,12-octadecadienoic acid) and include 9,10-EpOME and 12,13-EpOME. They are synthesized by cytochrome P450 monooxygenases (CYPs) and degraded by soluble epoxide hydrolase (sEH). Although EpOMEs are well known to play crucial roles in mediating various physiological proc...
Article
Full-text available
Sterile insect release technique (SIT) is effective for eradicating quarantine insects including various tephritid fruit flies. When SIT is used for fruit flies, it is challenging to remove females from sterile males due to oviposition-associated piercing damage. This study developed a sex transition technique by feeding double-stranded RNA (dsRNA)...
Article
Eicosanoids mediate both cellular and humoral immune responses in insects. Epoxyeicosatrienoic acids (EETs) are a group of eicosanoids containing epoxide formed by epoxygenase (EPX) activity of cytochrome P450 (CYP). Although EETs have been considered to mediate immune responses in some insects, their synthetic machinery was little understood in in...
Article
Phospholipase A2 (PLA2) hydrolyzes the ester bond of phospholipids (PLs) at sn-2 and releases free fatty acids and lysophospholipids that are subsequently changed into various signal molecules to mediate various physiological processes. Numerous PLA2s are known in various biological systems and can be divided into at least 16 groups. Although diffe...
Article
Phospholipase A2 (PLA2) hydrolyzes fatty acids from phospholipids at the sn‐2 position. Two intracellular PLA2s, iPLA2A and iPLA2B, have been found in Spodoptera exigua. Both are calcium‐independent cellular PLA2. Their orthologs have been found in other insects. These two iPLA2s are different in ankyrin motif of N terminal region. The objective of...
Article
Mating disruption by using sex pheromone is an ecofriendly alternative way to control insect pests. To be effective, large amounts of sex pheromone are needed, leading to a relatively high production cost. To reduce the cost for chemical synthesis of sex pheromone, yeast engineering technology has been devised. This study used a baker's yeast, Sacc...
Article
In insects, lipid digestion is controversial because insects have no bile salts to solubilize dietary lipids. One hypothesis is that a secretory type of phospholipase A2 (sPLA2) provides lysophospholipid (LPL) from dietary phospholipids (PLs). We identified a sPLA2, Se‐sPLA2, in beet armyworm, Spodoptera exigua that hydrolyses PLs at sn‐2. Our goal...
Article
Phospholipase A2 (PLA2) hydrolyses phospholipids at sn-2 position to release free fatty acids and lysophospholipids. Secretory type of PLA2 (sPLA2) has been found in many different animals including insects. Insect sPLA2s have been divided into venomous and nonvenomous PLA2s. A non-venomous sPLA2 (Se-sPLA2) has been identified in beet armyworm, Spo...
Article
Eicosanoids are oxygenated C20 polyunsaturated fatty acids that mediate various physiological processes in insects. Eicosanoid biosynthesis begins with a C20 precursor, arachidonic acid (5,8,11,14-eicosatetraenoic acid: AA). AA is usually released from phospholipids at sn-2 position by catalytic activity of phospholipase A2 (PLA2). Although various...
Article
The cotton bollworm, Helicoverpa armigera Hubner (Lep: Noctuidae), is an economically important pest of numerous major food crops worldwide. Protease inhibitors from plants, expressed constitutively in transgenic crops, have potential for pest management as an alternative to chemical pesticides. In this study, a protease inhibitor was isolated, pur...
Article
Full-text available
The Oriental fruit fly, Bactrocera dorsalis, has been designated as a main quarantine insect pest. Sensitive monitoring and diagnosis against B. dorsalis are required for early quarantine treatment. This study formulated a wax type dispenser containing methyl eugenol and biopesticide to attract and kill this insect. It also developed diagnostic PCR...
Article
Full-text available
Double-stranded RNA (dsRNA) has been applied to control insect pests due to its induction of RNA interference (RNAi) of a specific target gene expression. However, developing dsRNA-based insecticidal agent has been a great challenge especially against lepidopteran insect pests due to variations in RNAi efficiency. The objective of this study was to...
Article
Helicoverpa armigera is a significant agricultural pest and particularly notorious for its resistance to many types of common insecticides. RNA interference (RNAi) is a mechanism of post-transcriptional gene silencing and trigged by double-strand RNA (dsRNA), has become a widely used reverse genetics and potent tool for insect pest control. In this...
Article
Full-text available
The study of pectinase enzyme in potato leaf beetle that is the most important pest of potatoes consider as an effective way to develop control methods because this enzyme is important in degrading plant cell wall. Pectinase enzyme was studied in midgut of Colorado potato beetle (CPB). This enzyme was extracted from the midgut of larvae and adult o...
Article
Full-text available
The pistachio red bug, Lygaeus pandurus is one of the most destructive true bugs of pistachio in Kerman province. In order to better understanding about the nutritional physiology of the pest, after dissecting adults under stereomicroscope their midguts and salivary glands were removed. The tissues were homogenized and centrifuged and the supernata...
Article
The pistachio red bug, Lygaeus pandurus is one of the most destructive true bugs of pistachio in Kerman province. In order to better understanding about the nutritional physiology of the pest, after dissecting adults under stereomicroscope their midguts and salivary glands were removed. The tissues were homogenized and centrifuged and the supernata...
Article
Full-text available
In digestion, the red palm weevil, Rhynchophorus ferrugineus, has been adapted to overcome the plant cell wall barrier, specially lignocellulosic and pectic compounds, by producing cellulase and pectinase enzymes. Partial biochemical characterisations of cellulase and pectinase were determined in the larval digestive system of the pest. Larval midg...
Article
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Leptinotarsa decemlineata (Say) and Lasioderma serricorne F. are destructive pre-harvest and post-harvest pests of many plants in the family Solanaceae, and stored foodstuffs and non-food items, respectively. In this study, some biochemical characteristics of cellulase in the larval digestive tract of these pests were studied. Endo-β-1, 4-glucanase...
Article
The elm leaf beetle, Xanthogaleruca luteola, is a serious pest of elm trees in urban areas. Partial biochemical characterization of pectinases and cellulases was conducted using the larval digestive system of the pest. Midgut extracts from larvae showed optimum activity for pectinase and cellulase against pectin and carboxymethyl cellulose, respect...
Article
Full-text available
The cigarette beetle, Lasioderma serricorne, is a serious infesting pest of warehouses and retail stores thorough the world. Partial biochemical characterization of carbohydrases was performed in the larval digestive system of the pest. Midgut extracts from larvae showed an optimum activity for -glucosidase, -glucosidase and -amylase activity ag...
Article
Full-text available
Adults of Eurygaster integriceps were collected from the wheat field, dissected and their midguts and salivary glands were removed out. After centrifugation of the homogenates, the resulted supernatants were used as the source of enzyme. Pectinase activity was assayed using agarose plate and colorimetric assays. Pectinesterase and polygalacturonase...
Article
The alfalfa weevil Hypera postica is a serious economic pest in most alfalfa grown in many countries worldwide. Digestive -amylase and pectinase activities of larvae were investigated using general substrates. Midgut extracts from larvae showed an optimum activity for -amylase against starch at acidic pH (pH 5.0). -Amylase from larval midgut was mo...

Questions

Questions (11)
Question
We bought this kit (MEGAscript RNAi Kit) and following the instructions, we kept it at -20. But one of the micro tube that contains 10x binding buffer was cracked and we have lost buffer. It was supplemented with 1 ml of buffer. What are compounds of this buffer? 
Question
I am rearing Helicoverpa armigera at 25°C and 70% humidity. the insects eggs is not hatched. we have about 1000 eggs but after 10 days, we do not have any larvae. 
Question
I want to know what is difference between "Not determined" or "ND" with "Negative" or "-" in results in microbiology research. I studied a paper that a series of microorganisms were cultured on specific medium and results were shown as "+", "-" and "Not determined". 
Question
I want to know for dsRNA synthesis what kind of vectors is proper.  
Question
If the concentration of ethidium bromide is very high, what will have effects on visibility of bonds production on agarose gel.
What is the best alternative for ethidium bromide?
Question
Since the dsRNA triggers the RNAi machinery, What principles should be considered in gene silencing for dsRNA prepartion? Which region of target gene should be considered to produce dsRNA?
Question
I am looking for the nucleotide sequence of a particular gene in NCBI. But results obtained in large numbers and as predicted. Are sequences predicted to be trusted? How might one obtain these sequences? 
Question
What concentration of protein(mg/ml) is appropriate for gel filtration(Sephadex G-75) and ion exchange (DEAE-cellulose) chromatography? (Gel filtration column (120*1.5cm) and ion exchange column (25*2cm).)
Question
We have a column for gel filtration chromatography (120 cm*1cm) that we will use for enzyme purification. We want to load it by Sephadex G-75. What height (cm) of column packed with sephadex is needed? Is this an appropriate method?

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Project (1)
Project
Pistachio Pest Management