Mohammad Pourkazemi

• Professor
• Professor at Iranian Fisheries Science Research Institute (IFSRI)

Short term preservation of spermatozoa of salmonid fishes for practical use, increasing of reproduction potentials and reducing of expenses, has an important role in hatcheries. In this study spermatozoan motility and fertilization rate of 29 commercially cultured rainbow trout were examined. Semen was collected by abdominal stripping at the volume...

Abstract : In order to properly implement the project of sperm banking and sperm cryopreservation of sturgeon fish and Taggining, collecting the appropriate number of spawning stock of sturgeon from Sepidroud River. The breeders of sturgeon fish were caught in the spawning season in spring and autumn in the mouth of Sepidroud river. Preparation of...

Populations of sturgeon species have experienced a drastic decline in abundance and distribution in the Caspian Sea basin and are significant conservation species throughout their range. Whereas information relating to the genetic makeup of the population of those species is essential to the development of effective management plans, little informa...

The objective of the present work was to survey the effects of different sources of zinc supplements on serum enzymatic activities, the expression of genes related to antioxidant and immunity in rainbow trout (Oncorhynchus mykiss). Male rainbow trout were fed for 16 weeks on a diet supplemented with 40 mg kg−1 of mineral zinc supplement (MZ; ZnSO4·...

Dietary zinc as an essential micronutrient plays a major role in controlling the rate of fish performance and reproduction. Given the liver is an important organ for metabolism and some receptors in the liver are critical for maintaining fertility, it has been hypothesized that it may contributes to reproductive indices. In the present work, we inv...

Rainbow trout (Onchorhynchus mykiss) is one of the most important freshwater aquaculture fish in Iran. It is necessary to develop available molecular marker such as SNPs, which represent a useful tool in detecting adaptive signals in populations and also parentage assignment for O. mykiss. Genetic architecture of broodstock populations is important...

In present study, Analytical Hierarchy Process method was used for determining most important traits in the breeding program of Caspian Sea trout and two questionnaires were prepared in two stages. The first questionnaire was sent to the 43 Caspian Sea trout farms that were previously identified. In this questionnaire, farmers based on their knowle...

The genetic variation and population structure of the Persian sturgeon, Acipenser persicus (Borodin, 1897) was investigated by means of polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) analysis of the nucleotide dehydrogenase subunit 5 (ND5) of mitochondrial DNA (mtDNA). We compared these data with our previous study...

A 12‐week feeding trial was conducted to determine a suitable carbohydrate source and form for Pangasianodon hypophthalmus. Ten isoenergetic, isonitrogenous and isolipidic diets, including five carbohydrate sources (dextrin, potato starch, wheat starch, corn starch and sago starch) and two carbohydrate forms (raw and cooked), were formulated. Resul...

In this study, we genetically characterised Monogenea found on common carp in Iran. In total, 5603 parasites were recovered from 112 fish. The parasites were first identified morphologically as Dactylogyrus extensus, D. anchoratus, D. vastator, D. minutus, D. achmerowi and Gyrodactylus sprostonae. Representative samples were then subjected to seque...

In sturgeons, the induction of gynogenesis and sex reversal could be important for potential production of neomale sturgeon and all‐female progeny for caviar production. The aim of this study was sex reversal of ship sturgeon (Acipenser nudiventris Lovetsky, 1828) gynogen into male sex. Five‐month‐old gynogens were sex reversed into male by includi...

Caspian trout (Salmo trutta caspius Kessler, 1877) as endemic migratory fish, possessing great ecological economic and biodiversity value. Among the habitat drivers, temperature related effects on the genome were considered in this research. During the embryonic developmental stages, genomic alterations of single cells were assessed by Comet assay....

Differential susceptibilities to VHSV of two strains of Caspian trout (Salmo trutta caspius) (cultivated and wild parentages) were recently reported. We here describe the haematological changes in fish from the two strains following VHSV injection challenge. The infection was initially associated with clear reduction of various blood parameters in...

Caspian trout Salmo trutta caspius is an endangered subspecies of brown trout Salmo trutta which is native to the Caspian Sea. Restocking programmes have been established, but recent introduction of the rhabdovirus viral haemorrhagic septicaemia virus (VHSV) into Iranian rainbow trout farms connected to waterbodies supporting wild Caspian trout may...

Aquatic organisms present, not only simple sources of accumulated metal, but can interact with metals, altering their toxicity. Due to exposition of biosphere with metals, organisms have developed various defense mechanisms to protect themselves against adverse effects of these ions and their compounds. Metallothionein (MT) is one of that which rep...

Sargassum ilicifolium were collected from 18 stations of Sistan and Baluchistan coastal waters, washed out, dried and powdered then transferred to laboratory for biochemical analyzing of total protein (% DW) using Kejeldal method, amino acids using HPLC device, Crude fat (%DW) using Soxoleh method, Fatty acids with GC device after determination of...

در این بررسی تغییر ترکیبات تقریبی-شیمیایی گیاه دریایی سارگاسوم جمع آوری شده از سه ایستگاه در هر یک از شش منطقه ساحلی استان سیستان و بلوچستان به دست آمد. گیاه پس از جمع آوری شستشو، خشك و پودر شده به آزمايشگاه منتقل و پروتئين كل (درصد وزن خشك) با روش كجلدال، اسید های آمینه با بهره گیری از دستگاه HPLC، چربي كل (درصد وزن خشك) با روش سوكسله و اسيد هاي چ...

Aquatic organisms present, not only simple sources of accumulated metal, but can interact with metals, altering their toxicity. Due to exposition of biosphere with metals, organisms have developed various defense mechanisms to protect themselves against adverse effects of these ions and their compounds.MT is one of that which represent a critical m...

This study aimed to determine the sex specificity and expression pattern of foxl2 and cyp19a1a genes in great sturgeon Huso huso gonads during gonadal sex differentiation and development. The results revealed that foxl2 and cyp19a1a mainly expressed in female gonads and during gonad development the foxl2 and cyp19a1a mRNA expression is required for...

In this study, the polymorphism of myostatin gene (MSTN-1) as a candidate gene for quantitative traits was analyzed in the rainbow trout (Oncorhynchus mykiss). The association between the MSTN-1 polymorphisms and growth traits were also studied. Forty broodstock parents of rainbow trout from two farms including Yasuj and Shahrkord were selected and...

In this study the rainbow trout (Oncorhynchus mykiss) myostatin gene (MSTN) for detecting gene derived SNPs and performing genetic association study between MSTN polymorphisms and growth traits were analyzed. Twenty broodstocks of rainbow trout in Genetic and Breeding for coldwater Fishes center in Yasouj for generating full sibling were selected a...

Out of considerable environmental factors, temperature and its possible effects on life stages of Caspian trout investigated by natural incubation (8 ͦC) condition simulation comparing with cold (4 ͦC) and warm (12 ͦC) constant incubation temperatures in 3 well equipped incubators by water recycling systems. Green eggs triple treatments of wild and...

The effects of 14 days exposure to sublethal concentrations of endosulfan (10 and 40 μg L-1) were investigated in mRNA- P450 1A expression, antioxidant enzymes (SOD and CAT) activity and histopathological alterations of Persian sturgeon (Acipenser persicus) fingerlings with weights of 3-5 g. The results illustrated that the relative mRNA- P450 1A e...

This study represents a large-scale population genetic analysis of the stellate sturgeon, Acipenser stellatus, in the Caspian Sea. In total, 192 samples of adult stellate sturgeon were collected from four commercial catch stations in the Northern (Volga and Ural Rivers) and Southern Caspian Sea (estuary of Sefidrud and Gorganrod Rivers-Iran). Fifte...

Little is known about molecular mechanisms involved in gonad differentiation in sturgeon species. In non-mammalian vertebrates, sox9, dmrt1, cyp17a1 and ar are male specific genes in testicular differentiation and are highly conserved. In order to understand the mechanism underlying testicular development in sturgeon, we investigated sex steroids l...

BACKGROUND: Lactococcus lactis is one of the lactic acid bacteria that can be considered as a probiotic in Persian sturgeon. OBJECTIVES: The aim of the present study was to increase the resistance to Aeromonas hydrophila after feeding the L. lactis JF831150. METHODS: In the present study, first 60 fish were fed different doses of L.lactis JF831150...

The current study evaluated effects of dietary administration doses of Entrococcus faecium on the hematological factors, and disease resistance of Sander lucioperca against Aeromonas hydrophila infection. Fish were fed with dietary administration containing E. faecium doses including of (10¹⁰, 10⁸CFU/ g) for diet A1 and A2, respectively in a commer...

Rainbow trout (Oncorhynchus mykiss) is one of important salmonids that has been introduced to Iran. In hatchery fish populations, diminished genetic variability can be the result of different factors, such as a low effective number of parents for reproduction of the populations. The microsatellite DNA technique was used to detect the genetic variat...

The Persian Sturgeon (Acipenser persicus) is a commercially and valuable species, yet little is known of its population structure or genetic diversity, although commercial and bycatch harvests may be impacting multiple populations of this species. In this study, we estimated genetic diversity and population structure across six locations along the...

In sturgeon with sex determination system of female heterogamety, combination of gynogenesis and sex reversal could produce F₂ with high percentage of female. In this research, possibility of masculinization on gynogen progeny was studied. For this purpose, induction of gynogenesis was carried out in ship sturgeon using heterologous sperm. Verified...

In this study Primary cell lines were established from the culture of explants of caudal fin of Persian sturgeon, Acipenser persicus and the effect of media, temperature and FBS on fin explants culture in this fish was investigated. The margin of the caudal fin from a two year old specimen was aseptically cut, washed three times with PBS containing...

In this study Primary cell lines were established from the culture of explants of caudal fin of Persian sturgeon, Acipenser persicus and the effect of media, temperature and FBS on fin explants culture in this fish was investigated. The margin of the caudal fin from a two year old specimen was aseptically cut, washed three times with PBS containing...

In this study Primary cell lines were established from the culture of explants of caudal fin of Persian sturgeon, Acipenser persicus and the effect of media, temperature and FBS on fin explants culture in this fish was investigated. The margin of the caudal fin from a two year old specimen was aseptically cut, washed three times with PBS containing...

The mRNA expression of growth hormone (GH) gene in early development stages of Siberian sturgeon was investigated using RT-PCR method. Samples were collected from unfertilized eggs up to 50 days post hatched (dph) larvae in 11 different times. Ribosomal protein L6 (RPL6) transcripts were used as the internal standard during quantification of GH mRN...

A comparison between growth rates of this fish with its previous (bester) generation has been provided.

Most sturgeon species are close to extinction due to population overexploitation for meat and caviar. Bester is one of the promising hybrids in Acipenseridae to be produced for its meat and caviar. Its caviar is able to tolerate freshwater, grows faster than beluga and get matured in a shorter period of time. In this study we produced a F2 generati...

Aquaculture of species for human consumption is being considered as the first substitution to save wild aquatic populations during last decade. Big bester (♀Huso huso × ♂bester) is one of the highly appreciated hybrids among sturgeon hybrids for aquaculture. There is no published data on comparison between growth rates of this fish with its previou...

The micropyle number in the ova of sturgeon species from the South Caspian Sea was investigated.

Determination of the number of ovaum micropyle in thtee sturgeon species had been the main goal of this research.

In this research, the encoding cDNA of growth hormone (GH) was cloned from the pituitary gland of great sturgeon Huso huso (three adults: two females and one male, 7–9 years old, 70–90 kg, reared in concrete ponds). In order to obtain the great sturgeon recombinant GH expression in Pichia pastoris, the mature encoding cDNA was first cloned in TA ve...

The effect of starvation and re-feeding was investigated on growth, hematology and biochemical parameters in juvenile Persian sturgeon (Acipenser persicus). Three hundred and seventyfive fish (108±0.63 g) were divided into five feeding groups. The control group (C) was fed to satiation three times a day during the experiment. The four groups were s...

A 12-week feeding trial was carried out to evaluate the effects of different levels of dietary choline on growth rate, body composition and total liver lipid of juvenile Siberian sturgeon, Acipenser baerii. A semi-purified basal diet was formulated using vitamin-free casein and wheat gluten as a protein source, a mixture of animal and plant oils as...

The optimum dose of ultraviolet (UV) irradiation to produce gynogenetic Caspian salmon, Salmo trutta caspius, with emphasis on the "Hertwig effect" and photoreactivation (PR) were investigated. The sperm of Caspian salmon was irradiated with UV at 2010 ± 200 µw.cm-2 in different times including 0, 1, 3, 5, 8, 10, 15, 20, 25, 35 and 45 min and was a...

This study was conducted in order to investigate genetic diversity and population structure of pike-perch in the Northern part of Iran. For this purpose, 207 adult pike-perches from four regions of the Caspian Sea watershed (Talesh Coasts, Anzali Wetland, Chaboksar Coasts and Aras Dam) were collected. DNA was extracted and by using 15 pairs of micr...

Effects of dietary ascorbic acid (AA) supplementation were carried out on growth performance and physiological indices in juvenile beluga Huso huso fed different levels of dietary L-ascorbyl-2-polyphosphate as AA source consisting of 0 (control), 100, 200, 400, 800 and 1600 mg AA kg−1 diet for 16 weeks. No significant differences were found in grow...

A 12-week feeding trial was conducted to evaluate the optimum dietary inorganic copper (copper sulphate) in juvenile beluga, Huso huso. Eight semi-purified diets containing 1.1 (Cu1.0), 3.5 (Cu4.0), 7.1 (Cu7.0), 9.7 (Cu10), 13.1 (Cu13), 25.1 (Cu25), 49.9 (Cu50) and 195 (Cu195) mg Cu kg−1 diet in the form of CuSO4.5H2O were fed to fish of initial bo...

The effects of different periods of starvation (1, 2, 3, and 4 weeks) and subsequent re-feeding (over a 4 week) on the compensatory growth performance and insulin-like growth factor I (IGF-I) mRNA expression in liver and white muscle were investigated in juvenile Persian sturgeon (Acipenser persicus). First, a fragment of 617 nucleotides coding for...

Mahi sefid (Cyprinidae) is an economically important fish in the south Caspian Sea. Specimens was collected from the Lamir River, Sefid Rud, Shir Rud and Tajan Rivers and analyzed at 38 microsatellite DNA loci. Eight primers out of the thirty analyzed loci were polymorphic in all sampling regions. The highest observed heterozygosity (1) was in locu...

The late sexual maturity in sturgeon and the absence of morphological differences between males and females makes sex discrimination difficult. Identification of sex at an early life stage is of high interest in caviar production because it allows efficient selection of females. In this study, the ge-nome of 10 mature male and 10 mature female spec...

The kutum (Rutilus frisii kutum) is one of the economically valuable species of Caspian Sea. Two different races of this fish exist in the Caspian Sea, the spring race and the autumn race. The aims of this study were to analysis the population genetic structure and genetic diversity among and between populations of these races based on microsatelli...

Triploidy in grass carp, Ctenopharyngodon idella Valenciennes, 1844, was induced on fertilized eggs to compare cold and heat shocks. Two simplified methods explained for verification of triploidy in grass carp. The cold shock (7 ˚C) was given in three treatments for 30 min starting 2.0, 2.5 and 4.0 min after fertilization. In cold shock, the start...

In this study Amplified Fragment Length Polymorphism (AFLP) was applied to species identification of bester hybrids. Hybrids identification was performed by comparison of electrophoresis profiles with parental species. The simultaneous occurrence of diagnostic bands fixed in the parental species, genetic distance and identification and cluster anal...

Hybrids were produced by crossing female Caspian kutum Rutilus frisii kutum with male grass carp Ctenopharyngodon idella. The genome of eight larvae and parents were studied using micro-satellite markers for genetic evaluation and verification. After DNA extraction from parent fish and progeny, hybrid heritability of two loci was assessed using two...

The objective of this study was to analyse the population genetic structure of the Persian sturgeon (Acipenser persicus) in Sefidrud and Gorganrud rivers watershed based on the characterization of microsatellite markers during 2006 – 2008. 100 samples of Persian sturgeon were collected from two regions. Four microsatellite loci (Ls68, Spl168, Spl17...

Motility of spermatozoa was studied on 12 and 7 specimens of Acipenser persicus and A. stellatus, respectively. The density measured to be 2.22±0.65×10 9ml -1 in A. persicus and 2.21±0.55×10 9ml -1 in A. stellatus. Semen samples were diluted with two extenders containing tris 118mM, sucrose 23.4mM, pH=8, egg yolk (20%), dimethyl sulfoxide (15%) and...

Diploid gynogenesis was induced in ship sturgeon Acipenser nudiventris using UV-irradiated sperm from Siberian sturgeon Acipenser baerii. The optimal condition for the retention of the second polar body in ship sturgeon was determined to be 10 min after activation/fertilization in experiments. The temperature of cold shock and its duration were 2.5...

Sturgeons and paddlefish are among the most commercially valuable groups of fishes and include both low risk and highly endangered species. However, a recent bibliometric study on sturgeon and paddlefish research revealed that disproportionately little attention has been paid to those species that are endangered or face a high probability of extinc...

تنها در درياي خزر و حوضه آبريز آن كه يكي ،Cyprinus carpio ، دو تيپ تالابي و مصبي كپور معمولي وحشي از زيستگاههاي طبيعي اين گونه است، يافت مي شود. با توجه به اهميت مطالعه تنوع ژنتيكي و ساختار جمعيت در مديريت و حفاظت از گونهها، تنوع ژنتيكي دو تيپ مصبي و تالابي كپور معمولي وحشي با استفاده از روش مورد مطالعه قرار گرفت، در اين بررسي 80 قطعه ماهي كپور معم...

دو تيپ تالابي و مصبي كپور معمولي وحشي تنها در درياي خزر و حوضه آبريز آن كه يكي از زيستگاههاي طبيعي اين گونه است، يافت مي شود. با توجه به اهميت مطالعه تنوع ژنتيكي و ساختار جمعيت در مديريت و حفاظت از گونهها، تنوع ژنتيكي دو تيپ مصبي و تالابي كپور معمولي وحشي با استفاده از روش مورد مطالعه قرار گرفت، در اين بررسي 80 قطعه ماهي كپور معمولي وحشي بالغ از دو...

A study was conducted to ascertain the genetic structure and the level of heterozygosity of Acipenser persicus in the Cas-pian Sea. A total of 167 fish were randomly collected from Turkmenistan, Russia and two regions of Iran. The number of alleles of eleven microsatellite markers ranged from 3 to 21 and the mean observed values of heterozygosity w...

The Caspian Sea sturgeon stocks are facing dramatic decline and very limited brood stocks being caught annually for rehabilitation purposes. Gene pool construction is one practical approach for conservation of critical endangered rare sturgeon migrating for spawning into river. We established a sturgeon gene pool comprise of live specimens (brood s...

The effects of different periods of starvation and subsequent re‐feeding on the compensatory growth performance and IGF‐I mRNA expression in liver and muscle were investigated in juvenile Persian sturgeon. A fragment of 617 nucleotides coding for IGF‐I was cloned from liver, which included an open reading frame of nucleotides, encoding a 162 amino...

Lactic acid bacteria (LAB) are often found as sub-dominant microbial components in the gastrointestinal (GI) tract of fish and have positive effects on the host. They are generally promising candidates for probiotics and can act as substances to improving both native immune responses and growth performance of animals including fish. In the present...

Understanding the scale at which wild stocks of Persian sturgeon (Acipenser persicus) are genetically discrete is necessary for effective management of this commercially important species. Disomic DNA microsatellite markers are among the best tools for determining stock structure in fishes. As all sturgeon species have a polyploid ancestry of all s...

The Caspian Sea and its basin (e.g. Anzali wetland) is one of the natural habitats of wild common carp Cyprinus carpio. In this study the genetic structure of this species. In the south-west of Caspian Sea (the Anzali wetland) was investigated using PCR-RFLP analysis of D-loop region. Two hundred of mature fish were collected from 5 stations (40 in...

We estimated the genetic diversity and population structure of Persian Sturgeon across six regions of Caspian Sea along the coast of Iran using eight DNA microsatellite loci. Full genotypes were obtained for 195 individuals of Persian Sturgeon. Significant differences were detected between A. persicus specimens collected from Sefidrud River (P≤0.00...

Caviar-producing sturgeons belonging to the genus Acipenser are considered to be one of the most endangered species groups in the world. Continued overfishing in spite of increasing legislation, zero catch quotas and extensive aquaculture production have led to the collapse of wild stocks across Europe and Asia. The evolutionary relationships among...

Mitochondria1 DNA (mtDNA) control region sequences were analyzed to evaluate the population genetic structure of Persian sturgeon (Acipenser persicus) in Caspian Sea. A total of 45 specimens were collected from the different locations of the Caspian Sea. MtDNA control region was amplified using PCR. Direct sequencing was performed according standar...

AbstractA 3 × 4 factorial design was used to evaluate the dietary protein requirement and to determine the optimum dietary protein to energy (P/DE) ratio in sub‐yearling Persian sturgeon, Acipenser persicus, reared in the indoor system. Twelve experimental diets (40P16, 40P17, 40P18, 40P19, 45P19, 45P17, 45P18, 45P19, 50P16, 50P17, 50P18 and 50P19)...

The metallothionein, total protein, cadmium, mercury and copper contents were measured in soluble fractions (cytosol) of gills, kidney, intestine, muscle and liver of wild Persian sturgeon (Acipenser persicus) caught from the coast of the southern part of the Caspian Sea and a fish rearing pond (1-year old fish from a hatchery centre). The concentr...

The effects of starvation and subsequent re-feeding on compensatory growth performance, blood serum metabolites and IGF-ImRNA expression in liver and muscle were investigated in juvenile Persian sturgeon. Growth indices including body weight, SGR, CF, and HSI significantly decreased after starvation. However, after re-feeding sturgeons that were st...

Background and objective: Metal pollution has always been a major cause of contamination of environment and is considered as a major concern for food health. Worldwide, sturgeons are an important source of food and income. In this study, the human health risk due to consumption of caviar and muscle of Persian sturgeon (Acipenser persicus) were eval...

Identification of suitable molecular markers for correctly assigning progenies to their parents particularly during the early stages of development is vital for aquaculture breeding programs. Developing such markers in sturgeons, that have a polyploid ancestry, is particularly challenging because many markers exhibit polysomic inheritance. In the p...

63 s e s s i o n1: effectiveness of reproduction of fish species under the domestication process... sPerMaToloGical coMParison of neWly and 3 years cauGhT brooders of casPian sea TrouT (sAlmo truttA cAspius) The Caspian sea trout (Salmo trutta caspius) is distributed in southern basin of the sea and the natural stocks of this fish is critically end...

according to iUCn, ship sturgeon, Acipenser nudiventris such as the other Caspian sea sturgeons are in the red list as critically endangered (Cr). sturgeon with female heterogametic sex determination system (ZW) can recovered using induction of gynogenesis, namely by presence a female only, both female and male could be produced. For induction of g...

Concentrations of Zn, Cu and Mn were examined in caviar and muscle of the Persian sturgeon (Acipenser persicus) collected from coastal waters of south Caspian Sea during March and April, 2011. Mean Zn, Cu and Mn concentrations in caviar samples were 21.48, 2.05 and 1.66 μg g(-1) wet weight basis, respectively. The mean Zn, Cu and Mn concentrations...

The effect of starvation and subsequent re‐feeding to satiation on compensatory growth performance, insulin and blood serum values were investigated in juvenile Persian sturgeon (Acipencer persicus) with an average weight 108.04 ± 0.28 g (mean ± SEM) and in the same rearing condition over an 8‐week period. Sturgeons were allocated to one of five fe...

This study represents a larg-scale population genetic analysis of the common kilka in the Southern Caspian Sea (Gilan province). Totally, 60 individuals of adult common kilka from two seasons were sampled. Fifteen sets of microsatellite primers were developed from American shad (Alosa sapidissima), Pacific herring (Clupea pallasi), Atlantic herring...

Mahi sefid (Cyprinidae) is an economically important fish in the south Caspian Sea. Specimens was collected from the Lamir River, Sefid Rud, Shir Rud and Tajan Rivers and analyzed at 38 microsatellite DNA loci. Eight primers out of the thirty analyzed loci were polymorphic in all sampling regions. The highest observed heterozygosity (1) was in locu...

In order to have a sustainable management on Persian sturgeon as a highly commercial species in the South Caspian Sea, we need to identify its population structure and the level as well as its conservation status in their natural habitat. To develop a conservation program for this all Caspian Sea' sturgeon species it requires knowledge of its genet...