Mohammad Ali Obeid

Mohammad Ali Obeid
Yarmouk University | YU · Faculty of Pharmacy

Doctor of Philosophy (PhD) in Pharmaceutical sciences and and Drug Delivery

About

37
Publications
27,119
Reads
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382
Citations
Introduction
Dr. Mohammad Obeid has experience of teaching in Higher Education, conducting research and securing funding. The Research Group is applying Nano/Microfabrication techniques in Pharmaceutical drug delivery applications and manufacturing. More specifically, our areas of interest include: Microfluidics (Particle Formulation & Chip Manufacturing), targeted drug delivery, nucleic acids delivery, In vitro and In vivo studies, Electrospinning (Melt & Solution).
Additional affiliations
October 2014 - present
University of Strathclyde
Position
  • PhD Student
Description
  • Working a PhD student in Dr.Valerie Ferro's lab for the development of various delivery nanoparticles for the delivery of nucleic acids such as siRNA, miRNA, and Plasmid.
October 2014 - present
University of Strathclyde
Position
  • Research Assistant
Education
September 2003 - June 2008
University of Jordan
Field of study
  • Pharmacy

Publications

Publications (37)
Article
Non-ionic surfactant vesicles (NISV) are synthetic membrane vesicles formed by self-assembly of a non-ionic surfactant, often in a mixture with cholesterol and a charged chemical species. Different methods can be used to manufacture NISV, with the majority of these requiring bulk mixing of two phases. This mixing process is time-consuming and leads...
Article
Curcumin is a natural component extracted from the rhizomes of turmeric (Curcuma longa), a natural plat with known medicinal uses for more than 4000 years. Most turmeric therapeutic effects are attributed to curcumin, a yellow-coloured extract. Curcumin has received considerable attention due to its biological activities, such as its use in arthrit...
Chapter
Nanoscale technologies are crucial for the characterization and fabrication of biomaterials that are useful in targeted drug delivery systems. New materials enable the delivery of therapeutic agents to specific tissues and cells in order to treat a range of diseases. Bionanotechnology: Next-Generation Therapeutic Tools provides a quick overview of...
Article
Full-text available
Advanced innovations for combating variants of aggressive breast cancer and overcoming drug resistance are desired. In cancer treatment, ZnO nanoparticles (NPs) have the capacity to specifically and compellingly activate apoptosis of cancer cells. There is also a pressing need to develop innovative anti-cancer therapeutics, and recent research sugg...
Article
In this study, the use of a microwave reactor, which allowed high input of energy into a pressurised system in a short period of time, was investigated for preparation of lipid nanoparticles (LNPs). The aim was to optimise the formulation process by reducing manufacturing time. Two types of LNPs were prepared; non-ionic surfactant vesicles (NISV) a...
Chapter
Over the last few decades of research, the development of bionanotechnology was fueled by the need for a new type of nanomaterials with properties that are unmatchable with their counterpart synthetic nanoparticles. These characteristics include biodegradability, biocompatibility, and uneven shapes and formulations, with the ability to express curr...
Article
Full-text available
Background: Staphylococcus aureus is an important human pathogen, especially causing skin and soft tissue infections (SSTIs). Over the decades, the infections caused by antibiotic-resistant strains have often become life-threatening. Consequently, exploration and development of competent approaches to combat these serious circumstances are urgentl...
Chapter
There has been considerable attention paid to cutaneous stem cell in the area of regenerative medicine as a potential therapeutic goal for managing disorders of the skin and hair, possible use in quickening or supporting the process of wound healing, and numerous types of cancers related to the skin. Cutaneous stem cell plays an essential role in m...
Chapter
RNA interference (RNAi) is a posttranscriptional regulatory mechanism that employs siRNA. It typically results in the degradation of a target mRNA that encodes a particular protein. Treatment with siRNA therapeutics requires the use of an effective drug delivery system to assist in delivering these therapeutics into the cytoplasm of the transfected...
Chapter
Full-text available
Melanoma accounts for 4% of all skin cancer malignancies, with only 14% of diagnosed patients surviving for more than 5 years after diagnosis. Until now, there is no clear understanding of the detailed molecular contributors of melanoma pathogenesis. Accordingly, more research is needed to understand melanoma development and prognosis.
Article
Full-text available
Zebrafish is now among the leading in vivo model for cancer research, including prostate cancer. They are an alternative economic model being used to study cancer development, proliferation, and metastasis. They can also be effectively utilized for the development of cancer drugs at all levels, including target validation, and high-throughput scree...
Chapter
Triple-negative breast cancer (TNBC) is a subtype of breast cancer that lacks the expression of estrogen receptors and progesterone receptors (< 1%) and does not overexpress the epidermal growth factor receptor type 2. Almost 10%–20% of breast cancer patients are diagnosed with this type of breast cancer. TNBC is a highly invasive and resistant cla...
Chapter
Small interfering RNA (siRNA) has been identified as a useful therapeutic resource in cancer therapy. Molecular manipulation with siRNA has been reported to have high therapeutic potential on the cells with excessive gene overexpression or caused by mutation. However, siRNA therapeutic approaches are pursuing effective and efficient delivery system...
Article
Background: Virus nanoparticles have been extensively studied over the past decades for theranostics applications. Viruses are well-characterized, naturally occurring nanoparticles that can be produced in high quantity with a high degree of similarity in both structure and composition. Objectives: The plant virus cowpea mosaic virus (CPMV) has b...
Article
Full-text available
Carbon nanotubes (CNTs) are type of nano-materials which have interesting physicaland chemical properties such as thermal, magnetic, surface and chemical properties. These properties make CNTs as promising opportunities for biomedical research and applications. Because of these interesting properties, CNTs were investigated for their drug delivery...
Chapter
Full-text available
Genetic testing is focused on identifying chromosome, gene, or protein changes between healthy and diseased cells or person. Genetic test outcomes can either verify or rule out possible genetic conditions and help determine whether a person is likely to develop or pass a genetic disorder. There are currently more than 1000 genetic testing and many...
Chapter
16.1 Introduction to chemical modification of proteins The term protein cage designates a proteinaceous structure that self-assembles into a hollow structure. Protein cages are often classified as virus-like particles (VLPs) and nonviral cages, and are regarded primarily as well-defined hollow spherical capsule-like 3D structures with unmatched mon...
Article
Full-text available
Niosome nanoparticles can be prepared using different methods, each of which can affect the size and homogeneity of the prepared particles. The aim of this study was to establish if the method of preparation impacted on the prepared vesicles when loaded with a model protein and the type of immune responses induced to the vaccine antigen. Niosomes w...
Article
Full-text available
Natural products have been successfully used for treating various ailments since ancient times and currently there are several natural product based anti-cancer agents used as the main therapy to treat cancer patients or as a complimentary treatment to chemotherapy or radiation. Balanocarpol, which is a promising natural product that has been isola...
Article
Full-text available
Chemical modification of the surface of viruses, both the interior and the exterior, imparts new functionalities, that have potential applications in nanomedicine. In this study, we developed novel virus-based nanomaterials as a contrast agent for computed tomography (CT) imaging in vitro. The gold-coated cowpea mosaic virus (Au-CPMV) particles wer...
Article
Full-text available
Curcumin, a natural chemical compound found in Curcuma longa that has been used in antitumor and anti-inflammation applications, exhibits very limited water solubility and rapid in vivo degradation, which limits its clinical application. To overcome these limitations, niosome nanoparticles were prepared by microfluidic mixing for curcumin encapsula...
Article
Background: Surface modification of nanoparticles with targeting moieties can be achieved through bioconjugation chemistries to impart new targeted moieties. Various polymeric nanoparticles have been used for the formulation of nanoparticles such as naturally-occurring protein cages, virus-like particles, polymeric saccharides, and liposomes. These...
Article
Exosomes are nanovesicles secreted by many cells, including cancer cells. Extensive research has been carried out to validate potential applications of exosomes and to evaluate their efficiency in a wide range of diseases, including cancer. The current knowledge on the origin, biogenesis and composition of exosomes is described. This review then fo...
Article
Full-text available
RNA interference (RNAi) is an effective and naturally occurring post-transcriptional gene regulatory mechanism. This mechanism involves the degradation of a target messenger RNA (mRNA) through the introduction of short interfering RNA (siRNA) that is complementary to the target mRNA. The application of siRNA-based therapeutics is limited by the dev...
Article
Full-text available
Due to the increasing problem of drug resistance, new and improved medicines are required. Natural products and biotherapeutics offer a vast resource for new drugs; however, challenges, including the cost and time taken for traditional drug discovery processes and the subsequent lack of investment from the pharmaceutical industry, are associated wi...
Article
Full-text available
Malaria is an ubiquitous disease that can affect more than 40% of the world’s population who live with some risk of contracting this disease. The World Health Organization (WHO) has recently highlighted the high spread of this disease in Sub-Saharan Africa. Despite the considerable fall in mortality rate over the past decade, the development of res...
Article
Full-text available
Identifying novel compounds or improving bioavailability of drugs requires extensive screening, in vitro and in vivo testing and subsequent commercialisation. Traditional methods can be labour intensive and time-consuming. Use of modern technologies can reduce these challenges and is best achieved through collaboration with researchers speciali...
Article
Full-text available
Small interfering RNAs (siRNA) have a broad potential as therapeutic agents to reversibly silence any target gene of interest. The clinical application of siRNA requires the use of safe and effective delivery systems. In this study, we investigated the use of non-ionic surfactant vesicles (NISV) for the delivery of siRNA. Different types of NISV fo...
Article
Non-ionic surfactant vesicles (NISV) are colloidal particles that provide a useful delivery system for drugs and vaccines. One of the methods that is used for NISV preparation is microfluidics in which the lipid components dissolved in organic phase are mixed with an aqueous medium to prepare the particles through self-assembly of the lipids. In th...
Presentation
Full-text available
Physical Characteristics of Non-Ionic Surfactant Vesicles Prepared Using Different Manufacturing Methods- Oral presentation at the 43rd Controlled Release Society conference July/2017 Seattle-USA

Questions

Questions (14)
Question
Does anybody tried any of these systems for liposomes preperation:
Fluigent microfluidic system or Ever flow microfluidics?
or can you advice with suitable system for microfluidic production of liposomes?
Question
Can anyone suggest the best medium to grow the Human nasal epithelial cells?
I have RPMI, DMEM but I am not sure if they are suitable?
I got these cells from Sigma.
thanks
Question
Hi,
I am doing FACS experements to assess the GFP inhibition by siRNA. I am using A549 cells that are producing GFP and treated the cells with siRNA to inibit the GFP production. the cells then analysed by FACS.
How can I report the percentage of GFP inhibition? is it just as a percentage of median FITC of treated cells to the untreated cells?
thanks
Question
If I wrapped the 12 well plates after seeding the cells with alaminium foil and put it in the incubator, would that affect the growth of the cells? or would that affect the O2 levels that reach to the cells
thanks
Question
Hi
I am trying to measure the GFP inhibition when transfected with siRNA targeting the GFP.
For that I am trying to use our FACS Diva.
I have the A549 cells stably transfected with GFP and I am trying to target the GFP with siRNA using transfection reagent.
I have did that and measured the decrease in the flurecence using a plate reader and it work as I noticed a decrease in the flurecence but when I used the FACS it didnt work.
Can you please advice of a correct way of doing that with FACS.
What I did is gating the untreated cells and I was expecting to see a shift in the peak of the count vs FITC back with the treated cells as an indicator for the GFP inhibition... is that correct? and is the use of FITC correct with GFP?
can anyone please advice.
Regards
Mohammad Obeid

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Projects

Projects (3)
Project
this project aims to develop different nanoparticles based formulation to overcome bacterial resistnce
Project
I am working on the development of lipid based nanoparticles for effective delivery of siRNA in cancer treatment.