Mohammad Moazeni

Shiraz University · Department of Pathobiology

Comparative electric behavior of Cysticercus tenuicollis, Hydatid cyst and Coenurus cerebralis at the Very Low Frequency (VLF) region has been studied in detail. This investigation could be significant, because of the economic and public health importance of these parasitic infections in domestic animals. In this report, a single cell signal record...

Background: Establishment of experimental cystic echinococcosis (CE) in laboratory mice is required for the study of CE. Experimental CE may be established in primary or secondary forms; however, the primary form is more reliable for the study of CE. Aims: The aim of the study was to assess the possibility of establishment of primary CE in Balb/...

Background: Cystic echinococcosis (CE) is a life-threatening disease in many countries. Albendazole, as the drug of choice for medical treatment of CE, is accompanied by adverse effects and may be ineffective in 20%-40% of cases; hence, new and more effective compounds are urgently needed to optimize the management of the disease. This study was p...

Parasitic helminths are usually known as undesired pathogens, causing various diseases in both human and animal species. In this study, we explore supercapacitance/resistance behaviors as a novel probe for rapid identification and direct differentiation of Fasciola hepatica, Parascaris equorum (with and without larvae), Dicrocoelium dendriticum, Ta...

Helminths have always been studied as one of the critically annoying pathogens of parasite classes due to their adverse effects on the ecosystem of human life. They have the potency to negatively affect their hosts as points of disease, infection, cancer, and death, but in this study, we found interesting electronic properties in Fasciola hepatica,...

Background Novel and more efficient compounds are urgently required for medical treatment of cystic echinococcosis (CE). Germinative cell culture of Echinococcus granulosus could be used for anti-echinococcosis agent tests and other biological studies on CE. This study was performed to establish an in vitro cell culture model for E. granulosus germ...

The aim of the present investigation was to evaluate the impacts of Zataria multiflora aromatic water (ZMAW) and nano emulsion (NE) of Z. multiflora essential oil (ZMEO) on IL-4 (Th2-derived cytokine) and IFN-γ (Th1-derived cytokine) concentrations in mice with hydatidosis. Sixty four healthy BALB/c female mice were intraperitoneally injected by 15...

Background: Fasciola hepatica as an important parasite affects health of humans and animals in some tropical and subtropical areas of the world, including Iran. Little is known about the molecular diversity of Fasciola in Equidae. Therefore, this study aimed to characterize the genetic polymorphisms among parasites. Methods: Eight adult Fasciola...

Albendazole is known as the drug of choice for medical treatment of cystic echinococcosis (CE). Albendazole sulfoxide (ABZ-SO), as the main active metabolite of albendazole, has low efficacy in the disease due to low water solubility and poor absorptivity. PLGA nanoparticles (NPs) enhance the dissolution of poorly soluble drugs, and chitosan (CS) c...

Hydatid disease is an economic and public health concern in many countries. Currently, surgery is the main treatment option for hydatid disease. In the surgical treatment of hydatidosis, the use of scolicidal agents is very important due to inactivating live protoscoleces and preventing the recurrence of infection. Therefore, it is necessary to inv...

Background: New scolicidal agents and novel therapeutic drugs are essential for better management of the zoonotic infection, hydatid disease. This study evaluated the effect of a nanoemulsion (NE) of Satureja hortensis essential oil (SHEO) on protoscoleces and germinal layer of hydatid cysts. Methods: This study was conducted from July to October...

Background: Introduction of Taenia multiceps and T. gaigeri as two separate species have been recognized mainly on morphological grounds. This experimental study was conducted to test whether cerebral and non-cerebral forms of Coenurus cerebralis belong to one origin or they are originated from two different tape worms. Methods: Two groups of dogs...

Objective: There are various protoscolicidal agents for inactivation of protoscoleces of hydatid cysts before and during surgical operation. The present study was aimed to evaluate the protoscolicidal effect of two concentrations of Eucalyptus globulus on protoscoleces of Echinococcus granulosus sensu lato under in vitro condition and to compare i...

Résumé Objectif de l’étude Il existe plusieurs types de scolicides destinés à désactiver les scolex du kyste hydatique avant et pendant la chirurgie. Le but de cette étude était d’évaluer l’effet scolicide de deux concentrations d’huile essentielle d’Eucalyptus globulus sur les scolex de l’Echinococcus granulosus dans des conditions expérimentales...

Background The aim of this review was to establish the current epidemiology of taeniosis, cysticercosis and trichinellosis among humans and animals in Iran by carrying out a comprehensive assessment of published articles reporting on these foodborne zoonotic diseases across the country. Methods The Preferred Reporting Items for Systematic Reviews...

Some oribatid mites are intermediate hosts of anoplocephalid cestodes, where the cestodes complete their larval development in the bodies of these oribatid mites. In order to investigate the potential of oribatid mites as vectors of anoplocephalid cestodes, 64 species of these mites were collected from different pastures of Shiraz County (Fars Prov...

Aim: Hydatid disease during pregnancy may lead to various complications in both mother and child. Accordingly, enough knowledge is required to manage these complications. This study was done to evaluate the interaction between hydatid disease and pregnancy in laboratory mice. Methods: Twenty female BALB/c mice (Mus musculus) were divided into te...

In this study a relatively large and open top aquarium was designed, constructed and introduced as a suitable habitat for nutrition, growth and development as well as for egg laying and breeding of Galba truncatula under laboratory conditions. The soil and water used in the aquarium were collected from the locality in which the snails were collecte...

Hepatic injury is the major limitation of long-term albendazole administration in patients with cystic echinococcosis (CE), which could give rise to cessation of treatment. The objective of the present study was to evaluate the protective effects of Zataria multiflora aromatic water (AW) against the hepatic injury induced by long-term albendazole t...

Purpose: Albendazole is the drug of choice for chemotherapy of cystic echinococcosis (CE) but 40% of patients do not respond to it satisfactorily. With regard to the previously reported scolicidal activity of Zataria multiflora, this study was done to evaluate the efficacy of the co-administration of albendazole and Z. multiflora aromatic water (A...

In in vitro process of this study, protoscoleces of the hydatid cysts were exposed to two concentrations of nano emulsion (NE) of Zataria multiflora essential oil (ZMEO) (1 and 2 mg/mL) for 10 and 20 min. Viability of protoscoleces was confirmed using 0.1% eosin staining. For in vivo studies, sixteen laboratory mice were infected intraperitoneally...

Peganum harmala seeds extract has been previously reported to have antimicrobial and other medicinal properties. The aim of this study was to evaluate the ovicidal activity of the methanolic extract of P. harmala seeds against the eggs of F. hepatica. The phenolic compounds of the methanolic extract of P. harmala seeds were identified by HPLC analy...

Background and Objective: Hydatid cyst is the larval stage of the tapeworm Echinococcus granulosus which causes one of the most important zoonotic helminthic diseases worldwide. Using scolicidal agents before surgery may prevent recurrence of infection, however many scolicidal agents may cause serious side effects. Previously it has been proven tha...

Hydatidosis is a medically and veterinary important parasitic disease that is endemic in many parts of the world. Unilocular hydatid cysts may develop in almost any part of the body. Up to 70% of hydatid cysts are located in the liver, followed by 25% in the lungs. Cerebral hydatidosis is an uncommon manifestation of the disease, occurring in less...

Sheep and goats serve as intermediate hosts for the canine tapeworm Taenia multiceps. The cysts produced by the intermediate stage of parasite are usually found in the cerebral hemispheres of small ruminants, and the resulting disease is commonly known as coenurosis. Coenurosis is clinically manifested in the form of various nervous symptoms, depen...

In this study, molecular characterization of Echinococcus granulosus sample obtained from water buffalo originating from southwest of Iran was performed using comparative sequence analysis of cox1 mitochondrial gene. DNA was extracted from protoscoleces removed from hydatid cyst from the liver of a 2-year-old male buffalo slaughtered in Khuzestan p...

Fasciola hepatica and F. gigantica are the causative agents of fasciolosis in domestic animals and humans. Based on the morphometric criteria, differential diagnosis between them is problematic. In addition, intermediate forms of Fasciola have been found in Iran, which makes the differentiation more difficult. The aim of the present study was to pr...

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##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##

Hydatidosis is one of the most important parasitic zoonoses with worldwide distribution. Scolicidal agents are usually essential during the surgical therapy of hydatid cysts for prevention of recurrence. There is still no scolicidal agent with high efficacy and low adverse side effects. New and natural scolicidal agents not only may be used for pro...

Fasciolosis is of considerable economic and public health importance worldwide. Little information is available on the ovicidal effects of anthelminthic drugs. The use of ovicidal anthelmintics can be effective in disease control. In this study, the effectiveness of the methanolic extract of ginger (Zingiber officinale) on the eggs of Fasciola hepa...

The phenolic compounds of Zataria multiflora extract, were identified by HPLC analysis. Gallic acid, catechin, caffeic acid, and quercetin were found to be the major phenolic compounds. Eighty healthy laboratory Balb/C mice were infected intraperitoneally by injection of 1500 viable protoscoleces and were divided into prevention (40 mice) and thera...

GC and GC-MS were employed to determine the chemical composition of the essential oil (EO) from Zataria multiflora aromatic water (AW). Thymol (66.9%), carvacrol (15.2%) and carvone (7.3%) were found to be the major EO constituents. Eighty laboratory Balb/C mice were infected intraperitoneally by injection of 1,500 viable protoscolices and were div...

This study was designed to evaluate and compare the infection rate and cyst formation in male Balb/c mice following intraperitoneal injection of protoscoleces and activated oncospheres. Protoscoleces were collected aseptically from the liver of naturally infected sheep. The eggs were obtained from three experimentally infected dogs and activated on...

Cerebral coenurosis is caused by Coenurus cerebralis, the larval stage of Taenia multiceps. The metacestode causes severe lesions in the brain and spinal cord of the intermediate host, so-called "gid" or "stagger" disease. Whereas, the non-cerebral coenurosis caused by Coenurus gaigeri, the larval stage of Taenia gaigeri, particularly affects goats...

The present study was designed to evaluate the biochemical profiles of C. tenuicollis in sheep. 36 cysts were collected from Iranian fat-tailed sheep slaughtered at the main abattoir of Shiraz (Southwest Province, Iran). Cysticerci were attached to the omentum, mesenteries and liver and transferred to the laboratory. All cyst fluids were centrifuge...

The present study assessed whether the genetic variation among different hosts (sheep and cattle) and geographical isolates (n= 28) of Dicrocoelium dendriticum from Iran is present based on mitochondrial (nad1) and ribosomal (ITS-2) DNA markers. Molecular analysis revealed the presence of at least ten and two distinct haplotypes in the NADH dehydro...

This study was designed to evaluate and compare the infection rate and cyst formation in male Balb/c mice following intraperitoneal injection of protoscoleces and activated oncospheres. Protoscoleces were collected aseptically from the liver of naturally infected sheep. The eggs were obtained from three experimentally infected dogs and activated on...

Hydatid cyst formation in rat Moazeni M, Mootabi Alavi A, Shayegh H 1- Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran *Corresponding author: email: Moazeni@shirazu.ac.ir Key words: Hydatid cyst, Zoonoses, Rat, Echinococcus granulosus. G R A P H I C A L A B S T R A C T: Introduction and Objectives: Hydatid...

Some of the metacestodes are not only zoonotic but are also responsible for severe tissue damage, reduction in milk and meat production, and considerable economic loss due to condemnation of the infected organs of the herbivorous animals. The aim of the present study was to determine the prevalence of Cysticercus ovis, Cysticercus tenuicollis, hyda...

To investigate the scolicidal effect of the Satureja khuzistanica (S. khuzistanica)essential oil from aerial parts of this herbal plant. The essential oil was obtained by hydrodistillation method. Gas chromatography (GC) and gas chromatography mass spectrometry (GC-MS) were employed to determine the chemical composition of the essential oil. Protos...

Background: Few anthelmintics are available for the treatment of hydatid disease caused by the parasite Echinococcus granulosus. The appearance of resistance to synthetic anthelmintics and the adverse side effects of chemical drugs, stimulated the research of alternatives, such as medicinal plants. In the present study, the scolicidal effect of met...

The present study compared the genetic variation among 19 different isolates of Fasciola hepatica from cattle and sheep in different areas of Iran using sequence data for mitochondrial DNA gene, the subunit 1 of cytochrome C oxidase gene (CO1). Four different CO1 genotypes were detected among F. hepatica isolates that showed five variable nucleotid...

Cystic echinococcosis (Hydatid disease) is a zoonotic disease caused by Echinococcus gran-ulosus. The aim of this study, was to evaluate the scolicidal activity of methanolic extract of Zingiber officinale (Rosc.) family Zingibe -raceae, against protoscolices of hydatid cyst. Protoscolices were collected aseptically from sheep livers containing hyd...

The two species of Fasciola hepatica and Fasciola gigantica are the causative agents of fasciolosis in domestic animals and humans. Based on the morphometric data, differential diagnosis between these flukes is problematic. In addition, intermediate forms of Fasciola have recently been found in the Northern Iran, based on morphometrical analysis. T...

In this study an attempt was made to develop an efficient, rapid, simple, and reproducible method for the in vitro viability test of Echinococcus granulosus eggs. The eggs were obtained from an experimentally infected dog and kept at 4°C until use. To prepare the dead or damaged eggs, the eggs were heated in hot water (69-72°C for 10 min), preserve...

Abstract: The present study was designed to evaluate the biochemical profiles of C. tenuicollis in sheep. 36 cysts were collected from Iranian fat-tailed sheep slaughtered at the main abattoir of Shiraz (Southwest Province, Iran). Cysticerci were attached to the omentum, mesenteries and liver and transferred to the laboratory. All cyst fluids were...

Hydatid disease is one of the most important helminthic diseases worldwide. Hydatid cysts may be found anywhere in the body. The most effective treatment of hydatid cyst is surgical operation. Spillage of live protoscolices during the operation is the major cause of recurrence. Instillation of scolicidal agent into hydatid cyst is the most commonly...

The trematodes Fasciola spp. and Dicrocoelium dendriticum are recognized as the most important zoonotic helminthic parasites of sheep, goat and cattle which are responsible for considerable economic losses to ranchers. This cross-sectional survey was carried out in the slaughterhouse of Bojnourd, Iran from March 2008 to March 2009. During this inve...

Surgery is still the main treatment for hydatid disease. Recurrence of the infection is one of the end points of surgery in treating the hydatid cyst which results from the dissemination of protoscolices-rich fluid. Installation of a scolicidal agent into the cyst is the most commonly employed measure to prevent recurrence. Many scolicidal agents h...

The most confident way for treatment of hydatid cyst is surgical operation. Spillage of the cyst contents during the operation is the major cause of recurrence after hydatid cyst surgery. Instillation of scolicidal agent into hydatid cyst is the most commonly employed measure to prevent this complication. In the present study, the scolicidal effect...

Hydatidosis in humans and animals is an economic and public health problem in many parts of the world, and surgery is still the main treatment for hydatid disease. One of the most important endpoints of hydatid cyst surgery is recurrence. The main cause of recurrence is dissemination of protoscolices during the surgical operation. Preoperative dest...

Fasciolosis is a cosmopolitan parasitic disease with considerable economic and public health importance. Fasciola hepatica is the major cause of fasciolosis in man and domestic animals. Although remarkable research works have been done around the optimum temperature and time required for miracidial development, little is known about the exact susce...

Since the meat of chicken is considered one of the sources of the human infection, this study was undertaken to compare the prevalence of toxoplasmosis in free-ranging with semi-industrial and industrial chickens ( Gallus gallus domesticus ) in Shiraz, Southern, Iran. 203 serum samples from free-ranging chickens of sub-urban districts, 50 serum sam...

Background: Fasciolosis is a worldwide disease with major economic and public health consequences. Early detection of the infection is important for the prevention and control of the disease. ELISA allows for early detection of fasciolosis in man and animals. Fasciolosis is caused by Fasciola hepatica and F. gigantica in man and domestic animals re...