Mohammad Jafarmadar

Mohammad Jafarmadar
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology

About

94
Publications
2,444
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576
Citations
Citations since 2016
28 Research Items
214 Citations
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2016201720182019202020212022010203040
2016201720182019202020212022010203040

Publications

Publications (94)
Article
Full-text available
Adult hematopoietic stem and progenitor cells (HSPCs) respond to bacterial infections by expansion to myeloid cells. Sepsis impairs this process by suppressing differentiation of stem cells subsequently contributing to an ineffective immune response. Whether the magnitude of HSPCs impairment in sepsis is severity-dependent remains unknown. This stu...
Article
Full-text available
Circulating microRNAs (miRNA) alterations have been reported in severe trauma patients but the pathophysiological relevance of these changes is still unclear. miRNAs are critical biologic regulators of pathological events such as hypoxia and inflammation, which are known to induce endoplasmic reticulum (ER) stress. ER stress is emerging as an impor...
Article
Full-text available
This manuscript emerged from a larger third-party funded project investigating a new poly-trauma model and its influence upon secondary sepsis. The present sub-study compared selected leukocyte subpopulations in the circulation and bone marrow after polytrauma in BALB/c versus CD-1 mice. Animals underwent unilateral femur fracture, splenectomy and...
Article
Full-text available
Sepsis remains a major challenge in translational research given its heterogeneous pathophysiology and the lack of specific therapeutics. The use of humanized mouse chimeras with transplanted human hematopoietic cells may improve the clinical relevance of pre-clinical studies. However, knowledge of the human immuno-inflammatory response during seps...
Poster
Traumatic hemorrhagic shock (THS) impairs tissue perfusion causing hypoxia and inflammation, which has shown to induce endoplasmic reticulum (ER) stress. ER stress triggers the unfolded protein response (UPR) aiming at resolving ER stress or inducing cell death if ER stress cannot be solved. Thus, the activation of the UPR can either contribute to...
Article
Background: Plasma-based resuscitation showed protective effects on the endothelial glycocalyx compared to crystalloid resuscitation. There is paucity of data regarding the effect of coagulation factor concentrates (CFC) on the glycocalyx in hemorrhagic shock (HS). We hypothesized that colloid-based resuscitation supplemented with CFCs offers a th...
Article
Full-text available
Abstract In polytrauma patients, the impact of splenectomy is equivocal, ranging from negative to protective. We investigated the impact of splenectomy on immune responses in the 1st-hit polytrauma alone and on survival in the post-traumatic sepsis (2nd hit). Female BALB/c mice underwent polytrauma (1st hit) consisting of either a) TH: femur fractu...
Article
Full-text available
Background aim: Reperfusion after hemorrhagic traumatic shock (HTS) is often associated with complications that are partly ascribed to the formation of reactive oxygen species (ROS). The aim of our study was to compare the effects of restrictive reperfusion (RR) to rapid full reperfusion (FR) on ROS formation and/or oxidative events. Materials and...
Article
Objectives: We tested whether resuscitation supplemented with a) rat adipose-derived stem cells (ASCs) or b) secretome (conditioned media) of ASCs can ameliorate inflammation, cell/organ injury, and/or improve outcome after Hemorrhagic traumatic shock (HTS). Interventions: Rats were subjected to HTS and a resuscitation protocol that mimics pre-h...
Article
Full-text available
Mitochondrial-derived reactive oxygen species have been deemed an important contributor in sepsis pathogenesis. We investigated whether two mitochondria-targeted antioxidants (mtAOX; SkQ1 and MitoTEMPO) improved long-term outcome, lessened inflammation, and improved organ homeostasis in polymicrobial murine sepsis. 3-month-old female CD-1 mice ( n=...
Presentation
Traumatic-Hemorrhagic Shock (THS) is characterized by impaired tissue perfusion causing hypoxia and induction of inflammatory response. These events are also known to induce Endoplasmic Reticulum (ER) stress and trigger the Unfolded Protein Response (UPR). UPR aims to resolve the ER stress and restore ER homeostasis, promoting cell survival and ada...
Article
Full-text available
Purpose: It has been suggested that patients with traumatic insults are resuscitated into a state of an early systemic inflammatory response. We aimed to evaluate the influence of hemorrhagic shock and resuscitation (HSR) upon the inflammatory response capacity assessed by overall TNF-α secretion capacity of the host compared to its release from c...
Data
Supplementary Fig. 1. Score sheet for a custom-developed mouse clinical assessment scoring system. The well-being of mice is assessed based on six endpoints every 12 hours (beginning at 12h post-CLP); tree severity grades (i.e. 0, 1 or 2 points) are assigned. Euthanasia is indicated when: score ≥8 and/or by inability to trigger the startle reflex a...
Article
Introduction: Hemorrhagic traumatic shock (HTS) and reperfusion is often associated with general inflammatory response and organ dysfunction. Recent preclinical studies have shown that APOSEC exerts cytoprotective and/or immunomodulating effects. We evaluated the therapeutic potential of APOSEC in a HTS model in rats. Methods: Rats were subjecte...
Article
Introduction: Sepsis deregulates early hematopoiesis and can shift stem cells into an "emergency program", but dynamics of this phenomenon are unclear. We aimed to characterize outcome-dependent changes occurring in the hematopoietic stem and progenitor cells (HSPCs) compartment during acute polymicrobial sepsis. Methods: Forty female CD-1 mice...
Conference Paper
Introduction: Altered mitochondrial function by excessive production of reactive oxygen species (ROS) has been considered an important factor in pathogenesis of organ failure in sepsis. Methods: We investigated effects of two specific mitochondria-targeted antioxidants (SkQ1: lipophilic; MitoTEMPO: hydrophilic) on outcome, inflammatory response...
Article
Background: The role of plasminogen activator inhibitor type-1 (PAI-1) in abdominal sepsis remains elusive. Objectives: To study the influence of inhibition and over-expression of PAI-1 upon survival in cecal ligation and puncture (CLP) sepsis. Methods: I) mice underwent moderate CLP and received 10mg/kg of either monoclonal anti- PAI-1 (MA-MP6H6)...
Article
Full-text available
Introduction Plasminogen activator inhibitor 1 (PAI-1) is a key factor in trauma- and sepsis-induced coagulopathy. We examined how trauma-hemorrhage (TH) modulates PAI-1 responses in subsequent cecal ligation and puncture (CLP)-induced sepsis, and the association of PAI-1 with septic outcomes. Methods Mice underwent TH and CLP 48 h later in three...
Data
Hematological response to TH-CLP. 3 week-old mice were subjected to trauma and hemorrhage (−48 h) followed by mild (23G) polymicrobial cecal ligation and puncture (CLP) sepsis at 0 h. At −48 h, −42 h, −36 h, −24 h, 0 h, 6 h, 12 h and 24 h, mice (6 per time point) were sacrificed and blood was collected and analyzed. RBC, red blood cell count; PLT,...
Data
Predictive accuracy of plasma PAI-1 for death. AUC, area under the curve; ROC, receiver operating characteristic; CI, confidence interval. *P<0.05. Plasma PAI-1 levels obtained were evaluated by the ROC curve to determine the predictive accuracy for the outcome as expressed by the AUC. The predictive accuracy of the ROC-AUC was defined as: 0.9–1 =...
Data
Prediction of TH-CLP outcomes based on body temperature. BT, body temperature; AUC, area under the curve; CI, confidence interval, NPV, negative predictive value for outcome; PPV, positive predictive value for outcome. 3 month-old female mice (n = 57) were subjected to trauma and hemorrhage (TH, −48 h) followed by cecal ligation and puncture (CLP)...
Article
Full-text available
Age/gender may likely influence the course of septic complications after trauma. We aimed to characterize the influence of age/gender on the response of circulating cytokines, cells and organ function in post-traumatic sepsis. We additionally tested if post-traumatic responses alone can accurately predict outcomes in subsequent post-traumatic sepsi...
Data
Pre-CLP phase: complete cell count in 15 month old female mice. A–C. Levels of circulating red blood cells (RBC), hemoglobin (Hb) and platelets (PLT) at −48 h, −24 h and 0 h prior CLP. D+E. Circulating neutrophils (NEU) and lymphocytes (LYM) at −48 h, −24 h and 0 h prior to CLP. SUR = alive on day 16, DIE = died until day 16 post-TH. Data presented...
Data
Post-CLP phase: complete cell count in 3 month old female mice. A–C. Levels of circulating red blood cells (RBC), hemoglobin (Hb) and platelets (PLT) at −48 h, −24 h and 0 h prior CLP. D+E. Circulating neutrophils (NEU) and lymphocytes (LYM) at −48 h, −24 h and 0 h prior to CLP. SUR = alive on day 16, DIE = died until day 16 post-TH. Data presented...
Data
Post-CLP phase: complete cell count in 3 month old male mice. A–C. Levels of circulating red blood cells (RBC), hemoglobin (Hb) and platelets (PLT) at −48 h, −24 h and 0 h prior CLP. D+E. Circulating neutrophils (NEU) and lymphocytes (LYM) at −48 h, −24 h and 0 h prior to CLP. SUR = alive on day 16, DIE = died until day 16 post-TH. Data presented a...
Data
Post-CLP phase: complete cell count in 20 month old female mice. A–C. Levels of circulating red blood cells (RBC), hemoglobin (Hb) and platelets (PLT) at −48 h, −24 h and 0 h prior CLP. D+E. Circulating neutrophils (NEU) and lymphocytes (LYM) at −48 h, −24 h and 0 h prior to CLP. SUR = alive on day 16, DIE = died until day 16 post-TH. Data presente...
Data
Schematic of the Composite Inflammatory Score Calculation in the pre-CLP (A) and post-CLP (B) phase of post-traumatic sepsis. Calculation of the Composite Scores served as a tool enabling general comparison of inflammatory responses (and organ dysfunction) among all studied age/gender groups. To effectively normalize single cytokine values in all i...
Data
Post-CLP phase: complete cell count in 15 month old female mice. A–C. Levels of circulating red blood cells (RBC), hemoglobin (Hb) and platelets (PLT) at −48 h, −24 h and 0 h prior CLP. D+E. Circulating neutrophils (NEU) and lymphocytes (LYM) at −48 h, −24 h and 0 h prior to CLP. SUR = alive on day 16, DIE = died until day 16 post-TH. Data presente...
Data
Post-CLP phase: complete cell count in 15 month old male mice. A–C. Levels of circulating red blood cells (RBC), hemoglobin (Hb) and platelets (PLT) at −48 h, −24 h and 0 h prior CLP. D+E. Circulating neutrophils (NEU) and lymphocytes (LYM) at −48 h, −24 h and 0 h prior to CLP. SUR = alive on day 16, DIE = died until day 16 post-TH. Data presented...
Data
Comparison of the Composite Inflammatory Score in different age/gender groups across two phases of post-traumatic sepsis. Separate score trajectories in 3 (A and C), 15 (B and D) and 20 (C and E) month old female and male mice at −48 h, −24 h, 0 h prior CLP, and 6 h, 24 h and 48 h post-CLP are provided. To enable an overview of inflammatory activat...
Data
Comparison of the Composite Organ Dysfunction Score in different age/gender groups across two phases of post-traumatic sepsis. Separate score trajectories in 3 (A and C), 15 (B and D) and 20 (C and E) month old female and male mice at −48 h, −24 h, 0 h prior CLP, and 6 h, 24 h and 48 h post-CLP are provided. To enable an overview of organ dysfuncti...
Data
Pre-CLP phase: complete cell count in 3 month old female mice. A–C. Levels of circulating red blood cells (RBC), hemoglobin (Hb) and platelets (PLT) at −48 h, −24 h and 0 h prior CLP. D+E. Circulating neutrophils (NEU) and lymphocytes (LYM) at −48 h, −24 h and 0 h prior to CLP. SUR = alive on day 16, DIE = died until day 16 post-TH. Data presented...
Data
Pre-CLP phase: complete cell count in 3 month old male mice. A–C. Levels of circulating red blood cells (RBC), hemoglobin (Hb) and platelets (PLT) at −48 h, −24 h and 0 h prior CLP. D+E. Circulating neutrophils (NEU) and lymphocytes (LYM) at −48 h, −24 h and 0 h prior to CLP. SUR = alive on day 16, DIE = died until day 16 post-TH. Data presented as...
Data
Post-CLP phase: complete cell count in 20 month old male mice. A–C. Levels of circulating red blood cells (RBC), hemoglobin (Hb) and platelets (PLT) at −48 h, −24 h and 0 h prior CLP. D+E. Circulating neutrophils (NEU) and lymphocytes (LYM) at −48 h, −24 h and 0 h prior to CLP. SUR = alive on day 16, DIE = died until day 16 post-TH. Data presented...
Data
Pre-CLP phase: plasma glucose levels in different age/gender groups. A–F. Glucose plasma levels at −48 h, −24 h and 0 h prior to CLP. SUR = alive on day 16, DIE = died until day 16 post-TH. Data presented as mean+SD. Dotted line represents normal values. In 3 m♀ SUR n≥15, in DIE n≥18 at all time points. In 3 m♂ SUR n = 7, in DIE n = 18 at all time...
Data
Pre-CLP phase: complete cell count in 15 month old male mice. A–C. Levels of circulating red blood cells (RBC), hemoglobin (Hb) and platelets (PLT) at −48 h, −24 h and 0 h prior CLP. D+E. Circulating neutrophils (NEU) and lymphocytes (LYM) at −48 h, −24 h and 0 h prior to CLP. SUR = alive on day 16, DIE = died until day 16 post-TH. Data presented a...
Data
Pre-CLP phase: complete cell count in 20 month old female mice. A–C. Levels of circulating red blood cells (RBC), hemoglobin (Hb) and platelets (PLT) at −48 h, −24 h and 0 h prior CLP. D+E. Circulating neutrophils (NEU) and lymphocytes (LYM) at −48 h, −24 h and 0 h prior to CLP. SUR = alive on day 16, DIE = died until day 16 post-TH. Data presented...
Data
Pre-CLP phase: complete cell count in 20 month old male mice. A–C. Levels of circulating red blood cells (RBC), hemoglobin (Hb) and platelets (PLT) at −48 h, −24 h and 0 h prior CLP. D+E. Circulating neutrophils (NEU) and lymphocytes (LYM) at −48 h, −24 h and 0 h prior to CLP. SUR = alive on day 16, DIE = died until day 16 post-TH. Data presented a...
Conference Paper
Full-text available
Background Plasminogen activator inhibitor type 1 (PAI-1) inhibits fibrinolysis and its plasma increases correlate with exacerbated sepsis mortality. However, its exact role in abdominal sepsis outcomes is unclear. We aimed to test the effects of fibrinolysis restoration upon survival during acute polymicrobial sepsis using a custom-developed anti-...
Article
Most experimental studies on hemorrhage and trauma are performed under anesthesia. We determined the effects of three commonly used anesthetic regimens on hemodynamics and organ damage under normal and hemorrhagic/traumatic shock (HTS) conditions in rats. Animals were anesthetized with ketamine/diazepam (K/D), ketamine/xylazine (K/X), or isoflurane...
Article
we have shown that hemorrhage/resuscitation altered gastrointestinal blood flow (GI-BF) and that gastric perfusion did not recover after resuscitation. This study aimed to determine the effect of nitric oxide (NO) supplemented resuscitation on the mean arterial blood pressure (MAP), GI-BF, and outcome after hemorrhagic shock. rats were subjected to...
Article
The objective of this study was to investigate early effects of peritoneal inflammation on the mitochondrial function in the vital organs, liver and kidney, and their relation to inflammatory and oxidative stress mediators. The study was performed on 14 domestic pigs. Peritoneal inflammation was induced in anesthetized pigs after a midline laparoto...
Article
Nitric oxide released from nitro-glycerine (NG) has been considered to improve the microcirculation. Septic conditions are, however, associated with excessive formation of nitric oxide (NO), which is formed from l-arginine by the inducible NO synthase (iNOS) activity. Since the characteristics and influence of NG-derived NO in sepsis remains unclea...
Article
The aim of this clinical and experimental study was to determine whether systemic neuron-specific enolase (NSE) is a useful early marker of traumatic brain injury (TBI) and whether NSE is affected by ischemia/reperfusion damage of abdominal organs. Our study included patients with and without TBI (verified by computerized tomography) admitted withi...
Article
Neuron-specific enolase (NSE) is an acknowledged marker of traumatic brain injury. Several markers originally considered reliable in the setting of traumatic brain injury have been challenged after having been studied more extensively. The aim of our experimental study was to determine whether NSE is a reliable marker of traumatic brain injury earl...
Article
S100B, an acknowledged marker of brain damage, is increased post-traumatically in plasma. The aim of this study was to investigate the diagnostic value of S100B release in experimental local extracranial ischemia and reperfusion. Anesthetized rats underwent laparotomy and ligation of the afferent blood vessels to the liver, gut, or kidney to achiev...
Article
D-lactate is produced by indigenous bacteria in the gastrointestinal tract. Mammals do not have the enzyme systems to metabolize D-lactate rapidly. The present study was designed to determine the kinetics of circulating D-lactate levels and to examine whether the severity of shock affects circulating D-lactate levels in rats subjected to hemorrhagi...
Article
Full-text available
S100B is an acknowledged marker of brain damage. However, trauma without brain damage also causes an increase in S100B. S100B concentrations are highest in multiple trauma patients with long bone fractures. Clinically, extensive long bone fractures are associated with haemorrhagic shock and haemorrhagic shock per se is associated with increased S10...