Mohammad Johari Ibahim

Universiti Teknologi MARA | UiTM · Faculty of Medicine

Lapatinib (LAP), a dual ErbB1 and ErbB2 tyrosine kinase inhibitor, is effective in ErbB2-positive breast cancer treatment but is associated with diarrhoea. ErbB1 is expressed in the intestine; thus, it is hypothesised that lapatinib inhibits normal ErbB1 function, causing diarrhoea. This study investigated the possible involvement of ErbB1 inhibiti...

Background: A complicated interplay between radiation doses, tumour microenvironment (TME), and host immune system is linked to the active participation of immune response. Objective: The effects of single targeted 2 Gy and 8 Gy gamma-ray irradiations on the immune cell population (lymphocytes, B-cells, T-cells, neutrophils, eosinophils, and mac...

Targeting the activation of immune checkpoints is recognized as an effective strategy for triggering anti-tumour immune responses in cancer cells. Cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) and programmed cell death protein 1 (PD-1) were identified as potential crucial targets for cancer treatment. Overexpression of CTLA-4 and PD-1 protei...

Cancer recurrence is often associated with the acquisition of radioresistance by cancer tissues due to failure in radiotherapy. The underlying mechanism leading to the development of acquired radioresistance in the EMT6 mouse mammary carcinoma cell line and the potential pathway involved was investigated by comparing differential gene expressions b...

Cervical cancer is the most common cancer and ranked as 4th in morbidity and mortality among Malaysian women. Currently, Magnetic Resonance Imaging (MRI) is considered as the gold standard imaging modality for tumours with a stage higher than IB2, due to its superiority in diagnostic assessment of tumour infiltration with excellent soft-tissue cont...

We presented a flow cytometry protocol to isolate and measure the cellular component of tumour immune microenvironment (TIME) of mice bearing breast cancer models. The immune cells infiltrating the tumour microenvironment (TME) have been getting more attention as the determination of their pro- or/and anti-tumorigenic activity contributes to cancer...

Lapatinib, an orally administered dual ErbB1 and ErbB2 tyrosine kinase inhibitor, is an FDA-approved treatment for ErbB2-positive breast tumour. However, diarrhoea is one of the major drawbacks for this treatment. Incidence of lapatinib-induced diarrhoea (LID) has been reported as high as 58-78% of treated patients, with 23.3-25% grade 3-4 cases. A...

Lapatinib, an orally administered small-molecule tyrosine kinase inhibitor (SM-TKI), is an effective treatment for ErbB2-positive breast cancer. However, its efficacy as one of the targeted cancer therapies has been hampered by several adverse effects, especially gastrointestinal toxicity, commonly manifested as diarrhoea. Although it can be genera...

Osteosarcoma (OS) is the most common bone cancer affecting children with a high-grade malignancy characterised by severe lung metastases. Targeting tumour cell surface antigens have proven effective as alternative cancer treatments. Natural killer (NK) cells efficiently recognize and kill tumour cells through several mechanisms including the expres...

Automated machine learning (AutoML) has been recognized as a powerful tool to build a system that automates the design and optimizes the model selection machine learning (ML) pipelines. In this study, we present a tree-based pipeline optimization tool (TPOT) as a method for determining ML models with significant performance and less complex breast...

Hepatocellular carcinoma (HCC) is considered as a complex liver disease and ranked as the eighth-highest mortality rate with a prevalence of 2.4% in Malaysia. Magnetic resonance imaging (MRI) has been acknowledged for its advantages, a gold technique for diagnosing HCC, and yet the false-negative diagnosis from the examinations is inevitable. In th...

We presented a development of a custom lead shield and mouse strainer for targeted irradiation from the gamma-cell chamber. This study was divided into two parts i.e., to (i) fabricate the shield and strainer from a lead (Pb) and (ii) optimize the irradiation to the mice-bearing tumour model with 2 and 8 Gy absorbed doses. The lead shielding was fa...

Oral route is one of the best minimally invasive methods for drug administration since it allows incorporation of nutrients and other exogenous molecules. In oral route, the small intestine is the main organ where the drug absorption occurs. Lapatinib is an oral administered drug, effective for ErbB2-positive breast cancer treatment however it is a...

Purpose: To evaluate the influence of iterative reconstruction (IR) levels on Computed Tomography (CT) image quality and to establish Figure of Merit (FOM) value for CT Pulmonary Angiography (CTPA) examinations. Methods: Images of 31 adult patients who underwent CTPA examinations in our institution from March to April 2019 were retrospectively c...

Radiotherapy plays an important role in cancer treatment; however, the incidence of radio-resistance remains become major challenges in cancer treatment. The presence of epithelial-mesenchymal transition (EMT) has become a clinical hallmark for cancer to relapse after radiation treatment. The aim of this study was to investigate the morphological c...

Abstract Background Synchrotron microbeam radiation therapy (MRT) is a new, evolving form of radiotherapy that has potential for clinical application. Several studies have shown in preclinical models that synchrotron MRT achieves equivalent tumor control to conventional radiotherapy (CRT) but with significantly reduced normal tissue damage. Method...

Biceps brachii (BB) is one of the muscles which are subjected to many variations. Recently, there is a lot of information about variations in origin but also some cases are reported on variations in insertion of BB. Presented herein was a case of BB muscle having both variant origin and insertion in the same muscle. The variant origin of BB was lon...

Inhibition of telomerase activity has emerged as a promising strategy to combat cancer cells, especially ones with no specific molecular targets such as triple negative breast cancer (TNBC). Cisplatin, a chemotherapeutic drug, is causing DNA damage while apigenin, a plant-derived antioxidant, induces apoptosis in various cancer cell types. Little i...

Tamoxifen is an effective treatment for estrogen receptor alpha positive (ERa+)-breast cancer, however patients who received the treatment for five years have greater mortality risk compared to those who did not receive tamoxifen. Furthermore, patients treated with tamoxifen developed resistance to the drug which mediated through p53 and PTEN. Ther...

The xCELLigence real-time cell impedance system uses a non-invasive and label-free method to create a cell index that is a composite measure of cell proliferation. The aim of this study was to evaluate xCELLigence against clonogenic assay (gold standard) for measuring radiobiological effects and radiation-induced bystander effects (RIBE). A radiobi...

Synchrotron microbeam radiation treatment (MRT) is a preclinical radiotherapy technique with considerable clinical promise, although some of the underlying radiobiology of MRT is still not well understood. In recently reported studies, it has been suggested that MRT elicits a different tumor immune profile compared to broad-beam treatment (BB). The...

ABSTRACT Introduction: Nuclear factor-kappa B (NF-kB), a transcription factor protein complex, is found in nearly all animal cell types. It is involved in cellular responses to various stimuli. Aberrant regulation of NF-kB and the signaling pathways that control its activity are involved in cancer development and progression, as well as in drug res...

The aim of this study was to identify genes and molecular pathways differentially regulated by synchrotron-generated microbeams (MRT) versus conventional radiotherapy (CRT) beams in vitro using cultured EMT6.5 cells. We hypothesized (based on previous findings) that gene expression and molecular pathway changes after MRT are different from those se...

Background High-dose synchrotron microbeam radiation therapy (MRT) has shown the potential to deliver improved outcomes over conventional broadbeam (BB) radiation therapy. To implement synchrotron MRT clinically for cancer treatment, it is necessary to undertake dose equivalence studies to identify MRT doses that give similar outcomes to BB treatme...

1.0 Introduction Cisplatin [cis-dichlorodiammine-platinum (II), CP],is an effective chemotherapy drug that has been used for the treatment of various cancers including sarcomas, small cell lung cancer, ovarian cancer, breast cancer, lymphomas and germ cell tumors. Cisplatin forms a platinum complex inside the cell by cross-linking with DNA. This wi...

A rapid method for quantification of vitamin E isomers (α-, β-, γ-, δ tocopherol and tocotrienols) was developed by using ultra performance liquid chromatography (UPLC) from human plasma. This method involved liquid-liquid extraction of plasma sample to extract the lipophilic portion for injection into UPLC system. The UPLC system was equipped with...

Vitamin E supplements containing tocotrienols are now being recommended for optimum health but its effects are scarcely known. The objective was to determine the effects of Tocotrienol Rich Fraction (TRF) supplementation on lipid profile and oxidative status in healthy older individuals at a dose of 160 mg/day for 6 months. Sixty-two subjects were...

T. crispa is a well known traditional medicinal plant used in India, Indonesia, Philippines and Malaysia. To date, there are numerous studies on this plant emphasizing it's antioxi-dant, antidiabetic, antimalarial and cosmetic effects. However, limited informations are available regarding T. crispa cytotoxicity potential. The objectives of this stu...

T. crispa is a well known traditional medicinal plant used in India, Indonesia, Philippines and Malaysia. To date, there are numerous studies on this plant emphasizing it's antioxidant, antidiabetic, antimalarial and cosmetic effects. However limited informations are available regarding T. crispa cytotoxicity potential. The objective of this study...

The free radical theory of aging (FRTA) suggests that free radicals are the leading cause of deteriorating physiologic function during senescence. Free radicals attack cellular structures or molecules such as DNA resulting in various modifications to the DNA structures. Accumulation of unrepaired DNA contributes to a variety of disorders associated...