Mohammad Vikas Ashraf- M.Sc. Biotechnology, Ph.D. Biotechnology (Pursuing)
- PhD Student at Baba Ghulam Shah Badshah University
Mohammad Vikas Ashraf
- M.Sc. Biotechnology, Ph.D. Biotechnology (Pursuing)
- PhD Student at Baba Ghulam Shah Badshah University
Looking for collaborators in the field of Antimicrobial Resistance and Plant Antimicrobials
About
10
Publications
2,520
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
72
Citations
Introduction
I am a researcher in protein engineering, focusing on beta-lactamase-mediated antimicrobial resistance (AMR). My work explores the role of phytochemicals in inhibiting enzymes that contribute to AMR and investigates the synergistic effects of plant-based therapies combined with antibiotics. Through this research, I aim to develop novel antibiotic therapies to combat multidrug-resistant infections and contribute to the fight against global AMR.
Skills and Expertise
Current institution
Publications
Publications (10)
Naturally, a wide range of genetic and environmental variables predominate, such as bacterial, viral and parasite infective entities that have been identified as carcinogenic bioagents. Many helminth and protozoan parasitic diseases are liable to cause human cancer. Conveniently, three trematode parasites viz. Schistosoma haematobium, Opisthorchis...
The Himalayan Mountains are one of the most remarkable geological wonders in the world. Given the enormous floral and faunal diversity, the Himalayas are one of the world’s ecological hotspots. At the same time, it is one of the most vulnerable ecosystems, which has been put to exploitation across all forms. The Himalayas serve as a major tourist a...
Concrete, although versatile and one of the pervasive construction materials, is susceptible tomcracking. Cracks allow harmful elements to enter, compromising durability, which ultimately resultsmin high replacement costs. While prevention of crack formation in concrete is quite impossible,mnumerous techniques are engaged to heal up the cracks incl...
The active ingredients produced by plants as secondary metabolites have always been in focus because of their multifocal activities on a host body. As far as the immune system is
concerned, any imbalance could result in a catastrophic health condition such as cancer, rheumatoid arthritis, cardiovascular disorders and hypersensitivity reactions. Pla...
Oxidative stress, driven by reactive oxygen, nitrogen, and sulphur species (ROS, RNS, RSS), poses a significant threat to cellular integrity and human health. Generated during mitochondrial respiration, inflammation, UV exposure and pollution, these species damage cells and contribute to pathologies like cardiovascular issues, neurodegeneration, ca...
The people of Jammu and Kashmir rely heavily on fish as a source of nutritional protein. Fishes also contribute significantly to the local economy of this area. However, several infectious disorders, some of which are brought on by helminth parasites, constitute a persistent threat to fish. The primary goal of the present review is to find out the...
Parasitic diseases affecting millions of people globally cause fatalities and incapacitating conditions. It is, therefore, essential to detect parasitic diseases by looking for the parasite/s or their specific proteases that they produce at different phases of their life cycles. Numerous symptoms and indicators can result from a parasitic infection...
Among all available antimicrobials, antibiotics hold a prime position in the treatment of infectious diseases. However, the emergence of antimicrobial resistance (AMR) has posed a serious threat to the effectiveness of antibiotics, resulting in increased morbidity, mortality, and escalation in healthcare costs causing a global health crisis. The ov...
Antibiotics have made formidable benefactions to human civilization and found extensive usage in infectious disease treatment. However, indiscriminate and overuse of antibiotics have evolved resistant bacterial strains, which is a cause of concern to healthcare set-ups all around the world. This situation has necessitated the need of introducing ne...
Questions
Questions (4)
The plasmid i am isolating is a low copy number plasmid. I am supplementing the broth culture with appropriate antibiotics. Even then the plasmid quantity i am getting is not satisfactory. The mini spin columns i am using are quite old and what i can conclude is that the silica membrane in the columns is not binding the DNA properly and the bulk isolate it getting washed away. I would like to know as how to enhance the efficiency of mini spin columns for plasmid prep.
Any other suggestions from your side would be highly appreciated
Regards
i am passaging my bacterial strain (DH5 alpha) without the selection marker and it has been post 12 passages but unknowingly it is still holding onto the plasmid and doesnt loose it, which it should have if talking rationally. Any suggestions?
I had kept my plant samples for shade drying. However, due to humidity the chopped samples have developed mold growth. kindly suggest me a reliable drying procedure for my plant samples & is drying at 40-50 degrees celsius feasible because the crude extract is meant for antimicrobial screening
Regards
Mohammad Vikas Ashraf
I have done Double disc synergy test of bacterial clones in presence and absence of EDTA. The plates show positive results for the presence of MBLs but still susceptible for carbapenems and surprisingly resistant to other beta lactams. I think if it was the other way around that could be explained but the kind of resistance pattern it shows is confusing.
