Mohammad Ahmadyousefi- PhD of Medical Biotechnology
- Hamedan University of Medical Sciences
Mohammad Ahmadyousefi
- PhD of Medical Biotechnology
- Hamedan University of Medical Sciences
About
21
Publications
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Introduction
I am deeply passionate about protein engineering and the development of pharmaceutical proteins to combat various diseases. Additionally, I have a keen interest in advanced bioinformatics. My curiosity extends to diverse areas such as biosensors, ELISA, nanobodies, aptamers, synthetic biology, nanoflowers, optogenetics, CRISPR, cell therapy, and gene therapy.
For a glimpse into my peer-review activities, visit my profile on Web of Science: https://www.webofscience.com/wos/author/record/1122159
Current institution
Publications
Publications (21)
In our previous study, we reported the design and recombinant production of the p28-apoptin as a novel chimeric protein for breast cancer (BC) treatment. This study aimed to evaluate the inhibitory activity of the chimeric protein against BC cells in vitro and in vivo. We developed a novel multifunctional protein, consisting of p28, as a tumor-homi...
This article provides a brief overview of DNA vaccines. First, the basic DNA vaccine design strategies are described, then specific issues related to the industrial production of DNA vaccines are discussed, including the production and purification of DNA products such as plasmid DNA, minicircle DNA, minimalistic, immunologically defined gene expre...
Active targeting using biological ligands has emerged as a novel strategy for the targeted delivery of diagnostic agents to tumor cells. Conjugating functional targeting moieties with diagnostic probes can increase their accumulation in tumor cells and tissues, enhancing signal detection and, thus, the sensitivity of diagnosis. Due to their small s...
Background:
Background: Ferritin has an important role in iron storage in the cells, and due to its nanocage structure and self-assembly properties, it has wide application prospects in nanobiotechnology.
Methods:
Methods: The maize (Zea mays) ferritin gene ZmFer1 was cloned and expressed in Escherichia coli BL21 (DE3) for the first time. Change...
Protein nanocages resemble natural biomimetic carriers and can be engineered to act as targeted delivery systems, making them an attractive option for various drug delivery and biomedical applications. Our research investigated the genetic link of a specific anti-HER2 peptide (LTVSPWY) to the exposed N-terminal region of the maize (Zea mays) ferrit...
Proven RNA-based medications have elevated the demand for targeted nucleic acid-based agents. In this context, nucleic acid aptamers represent an appropriate class of molecules. They are single-stranded nucleic acid molecules that bind to a target molecule with high affinity and specificity. Aptamers have a number of advantages over antibodies incl...
Many antibiotics available at the clinic are natural products or derivatives of these products that originate from bacteria, fungi, plants, and animals. After the discovery of penicillin by Alexander Fleming in 1928, the discovery and extraction of antibiotics from natural sources expanded greatly. We can modify the chemical structure of natural an...
Plants are the inexhaustible source of biologically active compounds. For thousands of years, humans have been using plants to treat diseases. In the past decades, a large number of researches have made many efforts to extract medicinal molecules from plants to treat diseases. The use of pure medicinal compounds has advantages over the use of medic...
In this review, gene delivery and its applications are discussed in tissue engineering (TE); also, new techniques such as the CRISPR-Cas9 system, synthetics biology and molecular dynamics simulation to improve the efficiency of the scaffolds have been studied. CRISPR-Cas9 is expected to make significant advances in TE in the future. The fundamental...
HIV is a virus that targets and hijacks the immune cells of the host. It multiplies by attacking the helper T-lymphocytes. HIV has remained one of the most difficult and dangerous infections in the world due to the inability to find a successful treatment and a lack of access to medical care. When the virus reaches the body, dendritic cells are the...
Background:
Biofilm is a community of bacteria embedded in an extracellular matrix, which can colonize different human cells and tissues and subvert the host immune reactions by preventing immune detection and polarizing the immune reactions towards an anti-inflammatory state, promoting the persistence of biofilm-embedded bacteria in the host.
Ma...
Long non-coding RNAs (lncRNAs) have important roles in regulating the expression of genes and act as biomarkers in the initial development of different cancers. Increasing research studies have verified that dysregulation of lncRNAs occurs in various pathological processes including tumorigenesis and cancer progression. Among the different lncRNAs,...
Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), has been the world’s driving fatal bacterial contagious disease globally. It continues a public health emergency, and around one-third of the global community has been affected by latent TB infection (LTBI). This is mostly due to the difficulty in diagnosing and treating patients with T...
Recently, peptide-based materials have been applied to solving many therapeutic problems and have shown particular efficacy as cancer immunotherapies, including early diagnosis, prognostic predictors, and the treatment of cancer patients by interacting with dendritic cells (DCs) as part of the first line of immune defense. It has become more obviou...
During the past decade, accumulating evidence from the research highlights the suggested effects of bacterial communities of the human gut microbiota and their metabolites on health and disease. In this regard, microbiota-derived metabolites and their receptors, beyond the immune system, maintain metabolism homeostasis, which is essential to mainta...
Staphylococcus aureus is known as a common pathogen that colonizes 30% of healthy humans. Additionally, this bacterium can cause a number of serious infections, that is, endocarditis, bacteremia, pneumonia, wound, skin infections, and tissue abscesses. A variety of cellular and molecular pathways and targets are involved in response against S. aure...
Human colorectal cancer is the third most common cancer around the world. Colorectal cancer has various risk factors, but current works have bolded a significant activity for the microbiota of the human colon in the development of this disease. Bacterial biofilm has been mediated to non-malignant pathologies like inflammatory bowel disease but has...
Nucleic acid aptamers are promising recognition ligands for diagnostic applications. They are short DNA or RNA molecules isolated from large random libraries through the Systematic Evolution of Ligands by EXponential enrichment (SELEX) procedure. These molecules, with a particular three-dimensional shape, bind to a wide range of targets from small...
Questions
Question (1)
Hi all,
I want to display a nanobody on the surface of a protein nanocage. My nanocage is an icosahedron porous (9 nm pores) homomeric protein (60-mer) which is constructed of 20 trimeric building blocks. I selected the N-terminal of the nanocage building block to link my nanobody with a flexible linker (GGS)4 to construct Nanobody-Linker-Nanocage. But when I modeled the trimeric building block with Modeller and Galaxy-homomer tools my nanobody appeared inside the protein nanocage. I found that a flexible region was in the C-terminal of the nanobody and with the addition of flexible linker this region become more and more flexible. I deleted the flexible linker and modeled Nanobody-Nanocage, but again the nanobody appeared inside the nanocage even without the flexible linker. I used a rigid linker and the problem was repeated again.
Can anyone help me to display my nanobody on the surface of my protein nanocage!
I attached the figure of my model. A GFP-Nanocage model was also provided for comparison.
Any help would be appreciated.
Thanks
