Mohammad Zainal Abidin

Universitas Gadjah Mada | UGM · Department of Animal Products Technology

Pantothenate synthetase from Escherichia coli (PSE. coli) catalyzes the ATP-dependent condensation of (R)-pantoic acid and β-alanine to yield (R)-pantothenic acid (vitamin B5), the biosynthetic precursor to coenzyme A. Herein we show that besides the natural amine substrate β-alanine, the enzyme accepts a wide range of structurally diverse amines i...

N-Substituted l-aspartic acids are important chiral building blocks for pharmaceuticals and food additives. Here we report the asymmetric synthesis of various N-arylalkyl-substituted l-aspartic acids using ethylenediamine-N,N'-disuccinic acid lyase (EDDS lyase) as a biocatalyst. This C-N lyase shows a broad non-natural amine substrate scope and out...

The information given to consumers is essential for them to choose one food product over another. The falsification of food contents on product labels is a widespread problem, especially with products related with pig or others prohibited food in Islam. Proving conclusively that fraud has occurred requires the detection and quantification of food c...

Access to vitamin B5 [(R)‐pantothenic acid] and both diastereoisomers of α‐methyl‐substituted vitamin B5 [(R)‐ and (S)‐3‐((R)‐2,4‐dihydroxy‐3,3‐dimethylbutanamido)‐2‐methylpropanoic acid] has been achieved using a modular three‐step biocatalytic cascade involving 3‐methylaspartate ammonia lyase (MAL), aspartate‐α‐decarboxylase (ADC), β‐methylaspart...

The study was designed to determine the potential of collagen hydrolysate produced from the skin of Kacang goat through chymotrypsin hydrolysis to be used as an inhibitor of angiotensin converting enzyme (ACE). This research was conducted in three replications, with the measured parameters include ACE inhibitory potential and collagen hydrolysate f...

Bating is one of the important stages in the tanning process that has a role as a determinant of leather properties. It is commonly carried out with the assistance of protease enzymes such as papain. By using surfactants, sorbitol and Sodium Dodecyl Sulfate (SDS), it can help the penetration of enzymes into the skin or hides. Thereby, the study aim...

Fish skin is one of the alternative materials to replace the common animal skin that used in the leather tannery. This research was carried out by vegetable tanning to the tilapia fish skin using selected concentration of fresh palm oil and used palm oil. The study aims to find out the physical quality of leather through the using of fresh and used...

Background and Aim: Gelatin is a dissolved protein that results from partial extraction of collagen, commonly from pig and bovine skin. There was no study on gelatin production from Kacang goat bones through enzymatic extraction. This study aimed to evaluate the chemical, physical, and functional properties of gelatin from bones of Kacang goat usin...

The fresh untreated tannery wastewater contains high organic and chemical pollutants. This study aims to determine the growth characters of Pseudomonas sp. LS3K in high polluted medium from fresh untreated tannery wastewater. The growth characterization was observed in the solid medium (containing 0;25;50;75 and 100% sterile tannery wastewater) and...

The leather industry's production activity affects liquid waste containing a high amount of organic and chemical compounds. This study aims to determine Bacillus cereus LS2 B's survival ability in the medium with the presence of fresh untreated tannery wastewater. The growth characterization was made and observed in the agar medium with 0; 25; 50;...

Background and Aim: Angiotensin-converting enzyme (ACE) is one of the inhibitory enzymes isolated from animals for the treatment of hypertension. ACE inhibitor (ACE-I) peptides can be obtained by hydrolyzing proteins from various animal tissues, including muscle and connective tissues. However, the study on ACE-I activity from collagen of Kacang go...

Article, pickle, and wet blue leather defects used for this study were fromthe Balai Besar Karet college, Kulit dan Plastik, D.I Yogyakarta (BBKKP YK), Indonesia. Meanwhile, the microorganisms in leather defects were grown in vitro at A-minimal mineral (MM) and B-lowest (1/200 v/v) nutrient media. A nitrogen source of 2% Sigma-Aldrich bovine gelati...

The tannery industry produces large amount of waste from many parts that cannot be used in making leather products. Process of minimizing the waste can be done by trimming the goat skin. The treatment of trimmed goat skin waste from tanneries can potentially produce value-added products. A study on enzymatic treatment of leather processing waste fr...

Carbon‐nitrogen (C‐N) lyases are enzymes that normally catalyze the cleavage of C‐N bonds. Reversing this reaction towards carbon–nitrogen bond formation can be a powerful approach to prepare valuable compounds that could find applications in everyday life. This review focuses on recent applications (last 5 years) of native and engineered C‐N lyase...

Methanol dehydrogenase (MDH) enzyme was purified from Bradyrhizobium japonicum USDA110 cell-free extract. The bacteria were grown in a culture medium with direct 0.5% methanol addition aimed to stimulates the MDH catalytic enzyme activation. Bradyrhizobium japonicum USDA110 MDH enzyme was purified by using 25 mM 2-(N-morpholine) ethanesulfonic acid...

This research aims to understand the effect of metal ions bioabsorption which belong on different elemental groups to the methanol dehydrogenase (MDH) enzymatic activity in nitrogen-fixing bacteria Bradyhizobium japonicum USDA 110. Ten metal ions with each have 30μM concentration were added to grow Bradyhizobium japonicum USDA 110 in 10-1 diluted n...

This research aims to understand the effect of metal ions bioabsorption which belong on different elemental groups to the methanol dehydrogenase (MDH) enzymatic activity in nitrogen-fixing bacteria Bradyhizobium japonicum USDA 110. Ten metal ions with each have 30µM concentration were added to grow Bradyhizobium japonicum USDA 110 in 10-1 diluted n...

DNA degraded during cooking cause some fragmentation and cleaved DNA into small fragment, consequenly the difficulty to isolate DNA and determine the species in the food matrix. The objectives of the reasearch was to isolate DNA, polymerazation of DNA fragment and detection of the pig species of commercial sausages after various cooking time and te...

ABSTRAK Kegiatan pengabdian yang berkaitan dengan pengolahan limbah peternakan sapi potong telah dilaksanakan oleh Kelompok Tani Ternak Sido Mulyo, Dusun Pulosari, Desa Jumoyo, Kecamatan Salam, Kabupaten Magelang. Hasil samping berupa limbah feses dan urine sapi diolah menjadi pupuk organik kompos dan pupuk cair. Kelompok Tani Ternak Sido Mulyo tel...

This research applied and evaluated a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using cytochrome b gene to detect pork contamination in meatballs from local markets in Surabaya and Yogyakarta regions, Indonesia. To confirm the effectiveness and specificity of this fragment, thirty nine DNA samples from different...

A polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) using BseDI restriction enzyme had been applied for identifying the presence of pork in processed meat (beef sausage and chicken nugget) including before and after frying. Pork sample in various levels (1%, 3%, 5%, 10%, and 25 %) was prepared in a mixture with beef and...