Mohamed Mahmoud Mostafa Elsokary

Benha University- illinois university, USA

A total of 636 1-day-old male Cobb chicks were randomly assigned to seven treatments. The chicks were offered feed and water ad libitum throughout the experimental period. The first three groups included different stocking densities of broiler birds (low stocking density, LSD: 23 kg/m²; medium stocking density, MSD: 34 kg birds/m²; and high stockin...

A total of 636 one-day-old Cobb chicks were randomly assigned to seven treatments. The chicks were offered feed and water ad libitum throughout the experimental period. The first three groups included different stocking densities of broiler birds (low stocking density, LSD: 10 birds/m ² ; medium stocking density, MSD: 15 birds/m ² ; and high stocki...

After the copulation process, some sperm start the long journey with an ultimate goal of fertilizing the oocyte. Inside the oviduct, sperm are stored, waiting for the ovulated oocyte where they bind to the apical surface of the oviduct cells, which in turn hold sperm to form a sperm nest. The essential functions of the sperm reservoir include attac...

Background and Aim: Despite many trials, buffalo embryos have poor cryosurvivability because of their high lipid content. L-carnitine was found to be a lipid-reducing agent when added to oocyte and embryo culture media. The study aimed to determine the most effective concentration of L-carnitine to improve the oocyte developmental competence and cr...

We aimed (I) to simulate an in vivo milieu, through establishing an in vitro paradigm to study sperm-oviductal interactions using different segments of oviduct, as well as different incubation media, and (II) to investigate spatial changes of oviductal gene expression. Two experiments were designed; one was to investigate the yield of oviduct aggre...

Objective: To assess the effects of melatonin and/or cysteamineon in vitro maturation, culturing and post-warming of buffalo embryos. Methods: Buffalo oocytes were classified into control, cysteamine (50 μM), melatonin (10 ng/mL) and cysteamine (50 μM) + melatonin (10 ng/mL) treatment groups. In experiment 1, previous treatments were added during i...

After mating, sperm move from the site of semen deposition towards the oviduct, where sperm are stored before fertilization. Much of the literature indicates that oviduct glycans bind sperm to retain them in the oviduct reservoir. We have discovered that a specific oviduct glycan on the epithelium (sulfated Lewis A trisaccharide; suLeA) binds bovin...

The aims of the present study were to assess the effects of cysteamine as an anti-oxidant on the rate of in vitro maturation (IVM) of buffalo oocytes (experiment 1), and their viability and nuclear status following vitrification (experiment 2). Immature oocytes with compact cumulus cells obtained from the ovaries of slaughtered animals were harvest...

Vitrification is a common method for cryopreservation of gametes and embryos. Although successful oocyte vitrification has been achieved in several animal species, subsequent progress is still limited especially in buffalo. To improve the effectiveness of vitrification of buffalo oocytes, two experiments were conducted. The first experiment evaluat...

Interest in oocyte cryopreservation has recently increased with the growing importance of in vitro embryo production, nuclear transfer and gene banking. Cryopreservation of oocytes collected from slaughtered animals of high genetic value and their subsequent utilization for production of transferable embryos may provide an opportunity to replenish...

Vitrification is a common method which is successfully used for cryopreservation of gametes and embryosin in several animal species, though subsequent progress is still limited,especially in buffalo. The present study aimed to evaluate the effect of vitrification process on nuclear status in matured buffalo oocytes. Morphology, viability, state of...