Mohamed Gad

Mohamed Gad
The German University in Cairo | GUC · Faculty of Pharmacy & Biotechnology

Professor of Biochemistry, Faculty of Pharmacy & Biotechnology

About

202
Publications
36,195
Reads
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2,080
Citations
Introduction
I am the former dean of faculty of pharmacy & biotechnology and Professor of Biochemistry at the German University in Cairo. I am leading a team that study the role of gasotransmitters in health and disease. We are particularly interested in cancer and cardiovascular diseases
Additional affiliations
May 2016 - present
The German University in Cairo
Position
  • Head of Faculty
August 2011 - August 2013
The German University in Cairo
Position
  • Exploring genetic susceptibility to cardiovascular disease in Egyptians: correlation to biochemical investigations
April 2007 - December 2012
The German University in Cairo
Position
  • Professor o Biochemsitry
Education
February 1988 - May 1991
The Medical College of Pennsylvania
Field of study
  • Biochemistry
February 1984 - June 1987
Faculty of Pharmacy , Cairo University
Field of study
  • Biochemistry
September 1978 - May 1983
Faculty of Pharmacy, Cairo University
Field of study
  • Pharmacy

Publications

Publications (202)
Article
Full-text available
Background Omega-9 fatty acids represent one of the main mono-unsaturated fatty acids (MUFA) found in plant and animal sources. They are synthesized endogenously in humans, though not fully compensating all body requirements. Consequently, they are considered as partially essential fatty acids. MUFA represent a healthier alternative to saturated an...
Article
Full-text available
Ozonated olive oil (OL) combines the therapeutic effects of both ozone and olive oil. However, it suffers from limited water solubility and poor transdermal permeation, which hinder its application in melanoma treatment. Nanocarrier host molecules, such as niosomes, were used to improve the water solubility, transdermal permeation, and anticancer e...
Article
Full-text available
Triple-Negative Breast Cancer (TNBC) is one of the most aggressive and hot BC subtypes. Our research group has recently shed the light on the utility of natural compounds as effective immunotherapeutic agents. The aim of this study is to investigate the role of a methoxylated quercetin glycoside (MQG) isolated from Cleome droserifolia in harnessing...
Article
Full-text available
Background Cardiovascular disease (CVD) remains the major cause of death worldwide. Most CVD can be prevented by addressing risk factors. Acute myocardial infarction (AMI) is an inflammatory disorder characterized by changes in several cytokines including the interleukins (ILs). Studies are running to evaluate the genetic variation in the inflammat...
Article
Full-text available
Long non-coding RNAs (LncRNAs) play a vital role in the process of malignant transformation. In breast cancer (BC), lncRNAs field is currently under intensive investigations. Yet, the role of lncRNAs as promising diagnostic and/or prognostic biomarkers and as therapeutic target/tool among BC patients still needs a special focus from the biomedical...
Article
Full-text available
Triple Negative Breast Cancer (TNBC) immunotherapy has recently shown promising approach. However, some TNBC patients presented with resistance. One of the reasons was attributed to the excessive release of cytokines at the tumor microenvironment (TME) such as Tumor necrosis factor alpha (TNF-α) and Interleukin-10 (IL-10). Fine regulation of these...
Preprint
Full-text available
Triple Negative Breast Cancer (TNBC) is one of the most aggressive and hot BC subtypes. Our research group has recently shed the light onto the utility of natural compounds as effective immunotherapeutic agents. The aim of this study is to investigate the role of a methoxylated quercitin glycoside (MQG) isolated from Cleome droserifolia in harnessi...
Article
Ephedrine, a sympathomimetic amine that exhibits several of adrenaline actions, is a plant alkaloid that is a common ingredient in several cold, asthma and narcolepsy treatment preparations, and in obesity management, and sport medicine. Its principal action mechanism relies on its direct adrenergic actions as well as indirect role that involves th...
Article
Background: Over the last few years, the number of people suffering from sleeping disorders has increased significantly despite negative effects on cognition and an association with brain inflammation. Objectives: We assessed memory deficits caused by sleep deprivation (SD) to determine the therapeutic effect of phosphodiesterase 4 (PDE4) inhibi...
Article
Full-text available
Background: Nitric oxide (NO) may have a dual role in cancer. At low concentrations, endogenous NO promotes tumor growth and proliferation. However, at very high concentrations, it mediates cancer cell apoptosis and inhibits cancer growth. High levels of NO have been observed in blood of breast cancer (BC) patients, which increases tumor blood flo...
Article
Full-text available
Introduction Vitamin D deficiency has been linked to cardiovascular pathologies including acute coronary syndrome (ACS). Polymorphisms in vitamin D associated genes have been confounding to vitamin D serum levels and pathological predispositions. 7-hydrocholesterol is a common precursor in cholesterol and vitamin D synthesis. DHCR7/NADSYN1 genetic...
Article
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This study aimed to unravel the regulatory role of noncoding RNAs (ncRNA) on the nitric oxide (NO) machinery system in triple‐negative breast cancer (TNBC) patients and to further assess the influence of NO‐modulating ncRNAs on TNBC progression, immunogenic profile, and the tumor microenvironment (TME). The results revealed miR‐939‐5p and lncRNA HE...
Cover Page
Full-text available
Hydrogen sulfide (H 2 S) is currently considered among the endogenously produced gaseous molecules that exert various signaling effects in mammalian species. It is the third physiological gasotransmitters discovered so far after NO and CO. H 2 S was originally ranked among the toxic gases at elevated levels to humans. Currently, it is well-known th...
Article
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Background B7-H4 is a novel immune checkpoint protein that negatively regulates T cell activation and function. It is overexpressed in many malignant tumors including breast cancer (BC). It was reported that the presence of the single nucleotide polymorphism rs10754339 (A/G) within the 3' UTR of the B7-H4 gene has a great influence on the risk and...
Article
Full-text available
Response surface methodology (RSM), based on the central composite design (CCD), was used for the systemic optimization of galactomannan (GAL) extraction from Trigonella foenum-graecum. GAL was reported to possess a variety of pharmacological effects and is commercialized as adjuvant therapy for diabetes, obesity, and hypercholesterolemia. Seven pr...
Article
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Introduction: Hydrogen sulphide (H2S) has been established as a key member of the gasotransmitters family that recently showed a pivotal role in various pathological conditions including cancer. Objectives: This study investigated the role of H2S in breast cancer (BC) pathogenesis, on BC immune recognition capacity and the consequence of targeti...
Article
Full-text available
Despite historically known as "junk" DNA, nowadays non-coding RNA transcripts (ncRNAs) are considered as fundamental players in various physiological and pathological conditions. Nonetheless, any alteration in their expression level has been reported to be directly associated with the incidence and aggressiveness of several diseases. MicroRNAs (miR...
Article
Full-text available
Background Triple-negative breast cancer (TNBC) patients are the least fortunate in terms of diagnosis, prognosis and treatment compared to other BC subtypes. Chemotherapy remains the standard treatment, with remarkable rate of resistance. Immunotherapy, on the other hand has shown promising approaches for this aggressive BC subtype. A critical det...
Article
Full-text available
Background Triple Negative Breast Cancer (TNBC) is the most belligerent subtype of BC. Yet, it is considered the best candidate for immunotherapy. As TNBC microenvironment is rich in CD8+ T lymphocytes (CTLs) and Natural Killer cells (NKs), the main key players in BC immune surveillance. During the antitumor immune-response, CTLs and NKs recognize...
Article
Background Triple Negative Breast cancer (TNBC) is a complex challenging disease that is not amenable to conventional treatment. Our research group has recently portrayed hydrogen sulphide (H2S) and its synthesizing enzymes, cystathionine β-synthase (CBS) and cystathionine-γ-lyase (CSE), as potent oncogenic mediators for TNBC progression. Recently,...
Article
Full-text available
The activating immune ligands, MICA/B, act as a “kill me” signal through the NKG2D receptor expressed on natural killer (NK) cells. Recently, the oncogenic miR-20a was found to mediate immune escape through repressing MICA/B levels in breast cancer (BC) cells. However, targeting miR-20a-MICA/B using natural compounds has rarely been investigated. O...
Article
Full-text available
Hydrogen sulphide (H2S), one of the gasotransmitters, has been reported to have a cardioprotective effect at its physiological levels in blood. Previous determinations of H2S levels in cardiovascular disease (CVD) patients suffered from diversity of analytical methods, different targeted chemical forms of the gas, and multitude of matrices assessed...
Article
Background TNBC is the most immunogenic tumor compared to other BC subtypes. Thus, clinicians shifted the treatment among TNBC patients to immunotherapeutic alternatives such as immune checkpoint inhibitors (ICB). Despite the success of ICB, resistance in some TNBC cases had recently appeared. Thus, adding a new factor in the immunotherpeutic equat...
Article
Full-text available
Megalin and cubilin are two receptors that mediate endocytosis of 25-hydroxyvitamin D (25(OH)D) for its final activation by hydroxylation. The aim of the present study was to evaluate the association of polymorphisms in megalin (rs2075252 and rs4668123) and cubilin (rs1801222 and rs12766939) with the circulating serum levels of 25(OH)D and with the...
Article
Cardiovascular diseases remain a major public health burden worldwide. It was reported that vitamin D protects the cardiovascular system through several mechanisms mainly by hindering atherosclerosis development. Genetic variations in vitamin D metabolic pathway were found to affect vitamin D levels. This study aimed at investigating the associatio...
Article
Full-text available
Triple‐negative breast cancer (TNBC) represents an aggressive breast cancer subtype. Among young females, TNBC is the leading cause of cancer‐related mortalities. Recently, long noncoding RNAs (lncRNAs) are representing a promising pool of regulators for tuning the aggressiveness of several solid malignancies. However, this still needs further inve...
Article
Full-text available
Historically, the long-held protein-centered bias has denoted 98% of the human genome as ‘Junk’ DNA. However, the current work has shifted the perception of such 'junk' transcriptional products to functional regulatory molecules. The recent surveillance of the human transcriptome has highlighted the pivotal role of such non-coding RNA (ncRNA) molec...
Article
Full-text available
This study focused on studying the impact of flavonoids isolated from Cleome droserifolia on HCC cell lines and to further unveil their possible impact on TP53 and its downstream tumor suppressor miRNAs. Three flavonol glycosides were isolated from C. droserifolia namely, Isorhamnetin-3-O-β-D-glucoside (1), Quercetin-3`-methoxy-3-O-(4``-acetylrhamn...
Article
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Dimethylarginine aminodehydrolase (DDAH1) and alanine glyoxylate aminotransferase2 (AGXT2) are two enzymes that contribute in asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) metabolism. Hence they affect production and bioavailability of eNOS-derived nitric oxide (NO) and consequently healthy blood vessels. The major aims o...
Article
Full-text available
Background: The activating immune ligand, MICA, acts as a “kill me” signal through the NKG2D receptor expressed on natural killer (NK) cells that are key players in the fight against breast cancer (BC). Shedding of MICA during BC progression acts as a formidable barrier against NK cells' immune-surveillance. Recently, miR-20a was found to mediate i...
Article
Background: Despite the significance of the traditional risk factors, recently published studies have suggested that inflammatory processes and variations in the genetics of the inflammatory system may participate in the initiation of atherosclerosis and its complications. Objective: To investigate the possible association between CD14 C(-260)T...
Conference Paper
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Introduction A recent surveillance of the human transcriptome has highlighted a pivotal class of RNA known as long non-coding RNA (lncRNAs). Myriad studies have reported the vital role of lncRNAs in oncology. However, the field is still in its infancy. lncRNAs are expressed in a highly cancer type-specific manner, thus could hold a lot of promises...
Conference Paper
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Introduction Metastasis is the main cause of cancer mortality. However, the triggering mechanisms and regulation of epithelial–mesenchymal transition (EMT) factors in the commitment of metastasis have not been well characterised. Spermatogenic Zip 1 (SPZ1) acts as a proto-oncogene and an upstream regulator of EMT during tumorigenesis. Material and...
Article
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Background: Myocardial Infarction (MI) is one of the leading causes of morbidity and mortality in Egypt and worldwide. Vitamin D deficiency has long been linked to incidence of cardiovascular diseases. Several factors were reported to contribute to serum vitamin D level including exposure to sunlight. However, genetic variations in the vitamin D m...
Article
Full-text available
Background: Thrombospondin (TSP) 1 and 4 are extracellular matrix glycoproteins that mediate cell proliferation, platelet aggregation and inflammatory response. Conflicting data addressed the possible contribution of TSP-1 and TSP-4 gene polymorphisms to acute myocardial infarction (AMI). Objective: Our study aimed to examine the association of...
Article
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Background: Connexin (Cx) proteins are the building blocks of gap junctions. Among these, Cx37 and Cx40 are expressed on vascular system and reported to have cardioprotective role. Linking polymorphisms in genes coding for Cx and coronary artery disease (CAD) risk showed conflicting results in different populations. None has been studied before in...
Article
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Background: Coronary artery disease (CAD) remains a major public health burden. Emerging research has suggested an association between vitamin D insufficiency and CAD. Vitamin D binding protein (VDBP) is the primary vitamin D carrier and many of its genetic polymorphisms are able to induce the expression of proteins with different affinities for t...
Article
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This study investigated the role of the T-786C polymorphism (SNP) in the 5'-flanking sequence of the endothelial nitric oxide synthase gene (eNOS) on its expression level in vascular endothelium with the ultimate goal of shedding more light on the mechanisms by which genetic variations of eNOS might affect the vascular level of nitric oxide (NO). S...
Chapter
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Until the beginning of the 1980s, nitric oxide (NO) was just a toxic molecule of a lengthy list of environmental pollutants such as cigarette smoke and smog. In fact, NO had a very bad reputation of being destroyer of ozone, suspected carcinogen and precursor of acid rain. However, by the early 1990s it was well recognized by the medical research c...
Book
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Acute Lymphocytic Leukemia is a widespread disease, mostly affecting childhood.The introduction of 6-mercaptopurine (6-MP), an antimetabolite drug, begins in the consolidation phase, and forms the backbone of remission therapy. 6-MP is a pro-drug, and requires activation to 6-TGNs which are false nucleotides incorporated into DNA, however, a compet...