Mohamed Fethi Diouani

Mohamed Fethi Diouani
Institut Pasteur de Tunis | PASTEURTN · Laboratory of Epidemiology and Veterinary Microbiology

VMD, MSc, CESS, PhD

About

62
Publications
11,716
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628
Citations
Introduction
He has a long-standing interest in clinical and eco-epidemiological research on human's and animal’s diseases and with special focus on zoonotic infectious diseases and emerging diseases. He is actually involved in several EU funded projects, international bilateral project's and National projects related to infectious diseases diagnostic and monitoring by advancing innovate approaches for Bio-sensing of proteomic, genomic and volatolomic diseases biomarkers.
Additional affiliations
April 1996 - present
Institut Pasteur de Tunis
Position
  • Veterinary Senior
Description
  • Use of new technology, nanotechnology, in infection diseases and veterinary medecine
Education
October 1986 - June 1991
Ecole Nationale de Médecine Vétérinaire de Sidi Thabet
Field of study

Publications

Publications (62)
Article
Full-text available
Quality and food safety represent a major stake and growing societal challenge in the world. Bacterial contamination of food and water resources is an element that pushes scientists to develop new means for the rapid and efficient detection and identification of these pathogens. Conventional detection tools are often bulky, laborious, expensive to...
Article
Despite the difficulties and the absence of credible scientific information concerning bovine tuberculosis in Algeria, our cross-sectional and inferential study, which is estimated to be a first in Algeria, affected three major semi-intensive regions in East of Algeria, by analyzing 21 holdings which grouped 516 cattle in an intensive and semi-inte...
Article
Introduction La leishmaniose cutanée (LC) est une affection fréquente en Tunisie ; elle touche aussi bien l’adulte que l’enfant. Elle est caractérisée par un grand polymorphisme clinique, parfois trompeur. Nous nous proposons de déterminer les particularités épidémiocliniques, moléculaires, thérapeutiques et évolutives chez l’enfant. Matériel et m...
Article
Introduction La LC endémique en Tunisie constitue un problème majeur de la santé publique. Les DPA sont les médicaments de référence. Quel que soit le traitement utilisé (DPA, thermothérapie ou cryothérapie) les cicatrices disgracieuses restent une crainte pour les patients et pour les médecins. Cela nous a mené à tester les LEDs, une technique com...
Article
Introduction La leishmaniose cutanée (LC) endémique au sud tunisien se caractérise par un grand polymorphisme clinique qui peut prêter confusion avec plusieurs pathologies dermatologiques. Le frottis dermique permet souvent de poser le diagnostic, en cas de doute l’histologie et/ou la PCR sont plus sensibles mais plus coûteuses et invasives. La der...
Article
Full-text available
Sensitive and reliable approaches targeting the detection of Leishmania are critical for effective early diagnosis and treatment of leishmaniasis. In this frame, this paper describes a rapid quantification assay to detect Leishmania parasites based on the combination of the electrocatalytic ability of gold nanoparticles (AuNPs) to act as a catalyst...
Article
Full-text available
The present paper reports an automated approach for the characterization and analysis of the behavioral of sand flies; the method used is based on Gaussian mixture model and Kalman filter for the detection and tracking of sand flies, and then the extraction of an optimized set of features from the trajectory of flight is performed for the classific...
Article
Introduction La leishmaniose cutanée (LC) endémique en Tunisie est caractérisée par un grand polymorphisme clinique parfois trompeur par sa localisation dont l’atteinte unguéale rarement décrite dans la littérature. Nous proposons de déterminer les caractéristiques de la LC dans cette localisation exceptionnelle. Matériel et méthodes Il s’agit d’u...
Article
Human cutaneous leishmaniasis, although designated as one of the most neglected tropical diseases, remains underestimated due to its misdiagnosis. The diagnosis is mainly based on the microscopic detection of amastigote forms, isolation of the parasite, or the detection of Leishmania DNA, in addition to its differential clinical characterization; t...
Article
Full-text available
Natural products are a very important source of bio-molecules for drug to treat infectious diseases. New antileishmanial agents are timely needed, due to leishmania toxicity, drug-resistant strains and high costs treatments. In this study, we report the chemical characterization and antileishmanial activity of oil extracts from three medicinal plan...
Article
The control of bovine tuberculosis (bTB) relies first on an optimal diagnosis of the disease. Several testshave been implemented for bTB detection which are generally complex, slow of use and relatively expen-sive especially in poor countries. A simple rapid, cost effective and efficient automated method for bTBassessment is still needed. Here, we...
Article
Background: Human echinococcosis is a neglected infectious disease affecting more than one million people globally. Its diagnosis is expensive and difficult because of lack of adequate resources in low-resource locations, where most cases occur. Methods: A group of volunteers diagnosed with the two main types of echinococcosis and corresponding...
Article
The present paper reports an automated approach for the characterization and analysis of the behavioral of sand flies; the method used is based on Gaussian mixture model and Kalman filter for the detection and tracking of sand flies, and then the extraction of an optimized set of features from the trajectory of flight is performed for the classific...
Article
The control of bovine tuberculosis (bTB) relies first on an optimal diagnosis of the disease. Several testshave been implemented for bTB detection which are generally complex, slow of use and relatively expen-sive especially in poor countries. A simple rapid, cost effective and efficient automated method for bTBassessment is still needed. Here, we...
Article
Here we report that superoxide, one of the hazardous reactive oxygen species (ROS), can be quickly detected by flexible organic field-effect transistors (OFETs) with the polyphenol-embedded conjugated polymer micro-channels. Rutin, one of the abundant polyphenols found in a variety of plants, was employed as a sensing molecule and embedded in the p...
Article
Full-text available
Abnormal electrical activity of heart can produce a cardiac arrhythmia. The electrocardiogram (ECG) is a non-invasive technique which is used as a diagnostic tool for cardiac diseases. Non-stationarity and irregularity of heartbeat signal imposes many difficulties to clinicians (e.g., in the case of myocardial infarction arrhythmia). Fortunately, s...
Cover Page
Full-text available
La tuberculose est une maladie infectieuse, contagieuse, commune à l’Homme et à de nombreuses espèces animales. Elle est due à diverses espèces bactériennes appartenant au Complexe Mycobacterium tuberculosis. En Tunisie, la forme extra-pulmonaire, notamment ganglionnaire, tend vers l’augmentation. Ceci pourrait être expliqué par une contamination d...
Book
Full-text available
La tuberculose est une maladie infectieuse, contagieuse, commune à l’Homme et à de nombreuses espèces animales. Elle est due à diverses espèces bactériennes appartenant au Complexe Mycobacterium tuberculosis. En Tunisie, la forme extra-pulmonaire, notamment ganglionnaire, tend vers l’augmentation. Ceci pourrait être expliqué par une contamination d...
Article
During the time periods of June 2015 and from July to August 2016, sandflies were collected among seven collection sites of the three leishmaniasis endemic villages of Sidi Bouzid, Tunisia. A total of 690 sandflies were captured and identified (380 males and 310 females). Four species belonging to genus Phlebotomus (Ph.) and two species belonging t...
Article
Introduction La leishmaniose cutanée (L.C) endémique en Tunisie réalise des tableaux cliniques inhabituels par ses localisations et ses complications redoutables. Nous rapportons une série de 158 cas de lymphœdème invalidant des membres inférieurs (M.I) compliquant une L.C. Matériel et méthodes C’est une étude rétrospective menée au service de der...
Article
An effective electrochemical influenza A biosensor based on graphene-gold (Au) hybrid nanocomposite modified Au-screen printed electrode has been developed. The working principle of the developed biosensor relies on the measurement of neuraminidase (N) activity. After the optimization of experimental parameters like the effect of bovine serum album...
Article
In the present paper, the essential oil and the volatiles emitted in vivo by leaves of A. herba alba, which grows wild in center of Tunisia, were analyzed by GC-MS and SPME, respectively. The essential oil was characterized by the predominance of oxygenated monoterpenes (93.3) with α-thujone as the major compound (45.5) followed by β-thujone (11.4)...
Article
Full-text available
Introduction La leishmaniose cutanée (LC) endémique à Gafsa est caractérisée par son polymorphisme clinique, parfois trompeur. La localisation palmo-plantaire (PP) est très rare, jamais décrite dans la littérature chez l’adulte. Le but de notre travail était d’évaluer les caractéristiques épidémio-cliniques et évolutives de la LC dans cette localis...
Article
Introduction La leishmaniose cutanée (LC) à L. major est très endémique en Tunisie. Par contre, la leishmaniose muqueuse (LM) est exceptionnelle. Nous rapportons une série de 25 cas de LM chez l’enfant. Nous nous proposons de préciser le profil épidémio-clinique, thérapeutique et évolutif de cette localisation particulière. Matériel et méthodes No...
Article
Introduction La leishmaniose cutanée (LC) est une affection complexe qui constitue un problème inquiétant de la santé publique. Les dérivés pentavalents d’antimoine (DPA) constituent le traitement de référence de la LC en Tunisie. Ils s’avèrent avoir des effets secondaires graves de plus en plus fréquents. Cela nous a amenés à tester une autre alte...
Article
Tuberculosis is a worldwide disease considered as a major health problem with high morbidity and mortality rates. Poor detection of Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis remains a major obstacle to the global control of this disease. Here we report the development of a new test based on the detection of the major vir...
Conference Paper
Full-text available
Influenza is a viral infectious disease, which mainly affects the respiratory tract. It is considered as a source of many health problems and enormous socioeconomic disruptions because of the very high rates of morbidity and mortality. Early identification of influenza virus remains the most effective way to monitor the emergence of pathogenic stra...
Article
Neuroaminidase (NA) enzyme is a kind of glycoprotein that is found on the influenza A virus. During infection, NA is important for the release of influenza virions from the host cell surface together with viral aggregates and may also be involved in targeting the virus to respiratory epithelial cells. In this study, a model electrochemical influenz...
Conference Paper
Full-text available
This paper presents a method for detecting and tracking phlebotominaes that are vectors for many important diseases. A method based on the Gaussian mixture model is used for Foreground/Background classification. Then, the mathematical morphology operations are used to refine the classification results and eliminate areas that do not represent phleb...
Article
Full-text available
Abstract In this work an impedimetric immunosensor based on affinity immobilization method of a biotin labelled anti-human IgG antibody, used as a model system, was reported. The experimental procedure involves the growth of a self-assembled monolayer of a thiol (cysteamine) carrying terminal amine groups on gold electrodes. Glutarardehyde, a homob...
Conference Paper
Several antigens have been produced and/or secreted from the mycobacterium bovis. The agent of bovine tuberculosis (TB) is used to detect this disease in cattle through the serological ELISA test (a, b, c, d and e). In this work, we propose a novel approach to improve the diagnosis bovine tuberculosis. In order to select the antigens with top prior...
Conference Paper
Full-text available
Several antigens have been produced and/or secreted from the mycobacterium bovis. The agent of bovine tuberculosis (TB) is used to detect this disease in cattle through the serological ELISA test (a, b, c, d and e). In this work, we propose a novel approach to improve the diagnosis bovine tuberculosis. In order to select the antigens with top prior...
Conference Paper
Full-text available
Several antigens have been produced and/or secreted from the mycobacterium bovis. The agent of bovine tuberculosis (TB) is used to detect this disease in cattle through the serological ELISA test (a, b, c, d and e). In this work, we propose a novel approach to improve the diagnosis bovine tuberculosis. In order to select the antigens with top prior...
Conference Paper
Full-text available
Several responses in the form of serological tests ELISA (Enzyme Linked Immuno Sorbent Assay), IIF (Indirect Immuno Fluoresence) and DHST (Delayed Hyper Sensitivity Tests) can be used to detect leishmania parasite infection in dogs. In this paper, we propose a new method to select the most discriminative tests based on determinant criterion. So, th...
Conference Paper
Full-text available
This paper presents a method for the accurate detection of the positions of moving phlebotomenae (sand-flies), and the extraction of their complete shape. The proposed method is based on the background subtraction approach, with a statistical background model found using Gaussian mixture model. Furthermore, a method based on the maximization of int...
Article
Full-text available
Background Bovine herpes virus type 1 (BHV-1) still causes great economic loss to the livestock industry and trade because there aren¿t any available drugs that proved to be fully effective against it. In this study, the cytotoxicity and the antiviral activities of the Thymus capitata extracts were evaluated for the development of new, non toxic an...
Article
Full-text available
A gp63PCR method was evaluated for the detection and characterization of Leishmania (Leishmania) (L.) parasites in canine lymph node aspirates. This tool was tested and compared to other PCRs based on the amplification of 18S ribosomal genes, a L. infantum specific repetitive sequence and kinetoplastic DNA minicircles, and to classical parasitologi...
Article
Full-text available
Trypanosomiasis is a haemoprotozoan disease which affects both human and all species of domesticated mammal, especially Trypanosoma evansi in dromedaries. In the present study, early tool for prediction of Trypanosoma evansi infections related to seroprotein changes of dromedary in Tunisia was studied. Animals were distributed into 3 groups on the...
Article
Full-text available
A study was undertaken between November 2008 and March 2010, in the focus of cutaneous leishmaniasis of Central Tunisia, to evaluate the role of Psammomys obesus (n=472) and Meriones shawi (n=167) as reservoir hosts for Leishmania major infection. Prevalence of L. major infection was 7% versus 5% for culture (p=not signifiant [NS]), 19% versus 16%...
Conference Paper
A new rapid diagnostic tool, practical in field conditions without high-tech, viable and inexpensive can be regarded as a key enabling resolution of the problem of equity, Human Rights and Access to Care access. In this work, development of electrochemical biosensors for leishmaniasis diagnosis was investigated. The example of canine leishmaniasis...
Article
Full-text available
A follow-up study of 917 dogs was undertaken between 1994 and 1995 in the focus of visceral leishmaniasis in northern Tunisia. It permitted to assess the demography of the dog population, the importance of canine leishmaniasis (CL) and the determinants of seropositivity and mortality of dogs. Canine population was stable through time with an input...
Article
Avian Influenza Virus (AIV) infections are a major cause of mortality and rapid identification of the virus has important clinical, economical and epidemiological implications The traditional methods for avian influenza virus diagnostic are Enzyme Linked Immunosorbent Assay (ELISA) and Reverse Transcriptase Polymerase Chain Reaction (RT–PCR) whic...
Article
The rabies constitutes one of the most dangerous viruses causing many death cases every year. Each year approximately 55,000 people die of rabies, with high percentage of children. High percentages (99%) of the registered cases were in Asia and Africa. In order to fight this dangerous disease, many techniques are usually used for diagnostic but are...
Article
The efficiency of an inactivated tissue culture rabies vaccine produced on BHK-21 cells, according to an in-house developed process, was evaluated and compared to a commercial cell-tissue culture vaccine (Rabisin). Fifteen experimental dogs from local common breed were duly conditioned during a quarantine period, then vaccinated via the subcutaneou...
Article
Kinetics of antibody responses and protection against rabies were investigated after injection of a single dose of rabies DNA vaccine and compared to those induced by one or two injections of cell culture-derived vaccine in dogs issued from the common local breed and reared in experimental conditions. Rabies DNA vaccine administered intradermally b...
Article
Full-text available
Two techniques are currently used to evaluate the humoral immune responses to rabies vaccination: ELISA, which detects binding antibodies to viral antigens and the WHO reference rapid fluorescent focus inhibition test (RFFIT), which assays in vitro virus-neutralizing antibodies. In this study, we have comparatively evaluated antibody responses of d...
Article
Full-text available
Iso-enzymatic characterization of 19 Leishmania strains isolated from Tunisian dogs revealed that all correspond to Leishmania infantum MON-1. This confirms the role of dog as a reservoir of the commonest zymodeme responsible for human visceral leishmaniasis in the Mediterranean basin. However, and in spite of the high number of identifications, ma...
Article
Full-text available
Leishmania infantum MON-1:the only zymodeme isolated during canine leishmaniasis in Tunisia. Iso-enzymatic characterization of 19 Leishmania strains isolated from Tunisian dogs revealed that all correspond to Leishmania infantum MON-1. This confirms the role of dog as a reservoir of the com - monest zymodeme responsible for human visceral leishmani...
Article
This study reports on the evaluation of two L. infantum specific DNA probes for the diagnosis of canine leishmaniasis. The probes presented very satisfying performances in terms of specificity (100%) and predictive value of the positive result (100%). However, their sensitivity (35.3%) and the clinical complexity of canine infections render their u...
Article
Full-text available
RESUME Cette étude séro-épidémiologique a été réalisée dans la région de Medjez El Bab (nord-ouest de la Tunisie) et a concerné 180 chiens dont le statut a été déterminé lors de l'enquête. Ces chiens identifiés et prélevés ont été suivis pendant trois années consé-cutives afin de déterminer l'incidence ainsi que la prévalence de la maladie, utilisa...
Article
Full-text available
A study was undertaken between November 2008 and March 2010, in the focus of cutaneous leishmaniasis of Central Tunisia, to evaluate the role of Psammomys obesus (n=472) and Meriones shawi (n=167) as reservoir hosts for Leishmania major infection. Prevalence of L. major infection was 7% versus 5% for culture (p=not signifiant [NS]), 19% versus 16%...

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Projects (4)
Project
Tuberculosis is a worldwide disease considered as a major health problem with high morbidity and mortality rates. Poor detection of Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis remains a major obstacle to the global control of this disease. Here we report the development of a new test based on the detection of the major virulent factor of Mtb, namely the early secreted antigenic target 6-kDa protein or ESAT-6. A label free electrochemical immunosensor using an anti-ESAT-6 monoclonal antibody as a bio-receptor is described herein. Anti-ESAT-6 antibodies were first covalently immobilized on the surface of a gold screen-printed electrode functionalized via a self-assembled thiol monolayer. Interaction between the bio-receptor and ESAT-6 antigen was evaluated by square wave voltammetry method using [Fe(CN)6]3 −/4 − as redox probe. The detection limit of ESAT-6 antigen was 7 ng/ml. The immunosensor has also been able to detect native ESAT-6 antigen secreted in cell culture filtrates of three pathogenic strains of Mtb (CDC1551, H37RV and H8N8). Overall, this work describes an immune-electrochemical biosensor, based on ESAT-6 antigen detection, as a useful diagnostic tool for tuberculosis. We are working, in collaboration with local and international teams, to develop new innovative tools for TB diagnosis.
Project
Exploring and exploiting physio-pathogenesis in conjunction with electronics for best diseases comprehension and control.