Mohamed Chliyeh

Laboratoire de botanique, biotechnologie et de protection des plantes de Kénitra · Biology

In spring of 2012, olive-trees with crown dieback, root rot and defoliation were observed in two years old olive tree in commercial plantations of tree nurseries in Sidi Taibi and in twenty to fifty years old field trees in Souk El Arbaa ol- ive crops in Northwest of Morocco (Gharb area). The objec- tive of this study was to isolate the responsible...

Pestalotia fici was isolated from the leaves of the olive plants nurseries (Dahbia variety) existed in Sidi Taibi and from the olive trees in the region of Al Gharb (Moroccan Picholine variety). Koch´s postulate was verified by inoculating healthy Dahbia and Haouzia olive leaves. This species was used to inoculate healthy Picholine olive fruits and...

This work was carried out to study the synergetic effect of phospho-compost and phosphate-laundered sludge combined or not with consortia of arbuscular mycorrhizal fungi (AMF) on the growth parameters and yield of bean plants. The results showed that the highest plant length, number of leaves, flowers, and pods were noted in plants from substrates...

This study aimed to evaluate the genetic variability in the olive tree population (Olea europaea L.) of Ouezzane plantations and identify the performing clones who present interesting agronomic and technological characteristics. The results of studying of agrobiological and technological features of promising clones of olive trees are presented. Th...

The diversity of arbuscular mycorrhizal fungi (AMF) in the rhizosphere of Fig trees in the commune of Tizguite, Ifrane region (middle atlas region of northern Morocco) was presented in this study. Soil analysis revealed the presence of a diversified endomycorrhizal fungal community. The density of spores at the studied sites varied from 61 to 100 s...

The pathogenicity of two isolates (FS1 and FS2) of the Fusarium solani isolated from diseased chickpea plants harvested from two different regions from Morocco, which were identified morphologically and molecular, was evaluated as the severity, incidence and index of the disease. The results show that the two isolates tested were able to induce sym...

The argan tree, an endemic species in Morocco, has been exhaustively studied in its southwestern range but neglected in its northeastern territory. Thus, the present study sets as objectives the identification of arbuscular mycorrhizal fungi and the evaluation of the argan tree’s roots’ mycorrhization level in two stations: one in the region of Bén...

In poor soils, widely invaded by telluric fungi and subject to difficult environmental conditions (semi-arid and arid), the use of cultural techniques has become essential. Mycorrhizae are one of the promising strategies in the field of plant breeding and production. This symbiosis improves mineral and water nutrition and strengthens plant defenses...

Fungal isolates of Fusarium were collected from symptomatic chickpea (Cicer arietinum L.) plants growing in fields within Souk Tlat commune in the Gharb region. Morphological and molecular characterizations were performed of the fungal isolate N3 obtained from a chickpea plant. PCR amplification and sequencing of the internal transcribed spacer usi...

Despite the importance of arbuscular mycorrhizal fungi (AMF) within forest and agroecosystems, few data are available about how AMF communities are structured in the root zone of the argan tree. Some studies have characterized endomycorrhizal fungi population occurring in rhizosphere soils of argan trees grown in southwest of Morocco, numerous site...

Saffron cultivation is a viable alternative for marginal areas where low soil fertility and water availability severely limit the cultivation of other crops with higher water and input requirments. Under these conditions, arbuscular mycorrhizal fungi (AMF) are an essential alternative for maintaining fertility and water conservation, stimulating gr...

Saffron cultivation is a viable alternative for marginal areas where low soil fertility and water availability severely limit the cultivation of other crops with higher water and input requirments. Under these conditions, arbuscular mycorrhizal fungi (AMF) are an essential alternative for maintaining fertility and water conservation, stimulating gr...

The study aims to evaluate the effect of endomycorrhizal inoculum (arbuscular mycorrhizal fungi), phospho-compost and phosphate sludge in single (M, PC, PS) or dual combinations (PC+M, PS+M, PS+PC) compared to agricultural and Mamora soils (A and S) on the growth, flowering, and yield of tomato plants. Among the studied treatments, the substrates con...

Although some studies have characterized endomycorrhizal fungi population occuring in rhizosphere soils of argan trees grown in the southwest of Morocco, numerous sites of this area remained unexplored. There is a need to study additional soil samples which cover more sites of argan tree stands. Hence, we studied endomycorrhizae diversity and the m...

The great morphological similarities within the Colletotrichum genus and the lack of precision of classical identification methods have made the use of molecular methods mandatory. Despite the great diversity of species within the Colletotrichum genus, very few morphological characters are expressed. Morphological examination of the isolates of Col...

Surveys were carried out in April-May, 2017 in plots of barley located in different localities of northwest Morocco. Root and soil samples were taken under barley feet growing in these plots. The spores were isolated from the soil samples by wet sieving and the roots were stained with Cresyl blue. The observation of barley plants roots fragments fr...

The isolates TR-B 98 (2) and TR-B 98 (3) from the phosphate mines of Morocco and the isolate Trichoderma asperellum were tested for their ability to survive in the soil and stimulate the growth of wheat plants (soft wheat and durum wheat) and barley on a Mamora (SM) sand-based growing medium modified with increasing concentrations of natural phosph...

Biological and chemical treatments of seeds with a Trichoderma suspension or solutions of Thirame with 10% gelatin, 4% superphosphate, and 0.1% sucrose were able to reduce the severity, incidence and index of root rot in wheat and barley plants growing on a substrate inoculated with three pathogens responsible for root rot, Curvularia spicifera, Bi...

This study demonstrated beneficial effects of the symbiotic relationship between mycorrhizal arbus-cular fungi (AM) and olive trees. Young olive trees were treated with a composite mycorrhiza treatment with and without inoculation of the pathogenic Phy-tophthora palmivora. A non-inoculated control was also included. Mycorrhizal plants had greater m...

The attempts of argan tree (Agrania spinosa (L.) Skeels) reforestation programs, as a multi-use agro-forestry species deal with the mastering the production of superior quality seedlings in nurseries. Trichoderma spp. are well known as soil inhabitant and endophytic plant symbionts that are widely used as seed treatments to enhance plant growth. In...

Pure cultures of a large number of fungal isolates were obtained from anthracnose symptoms developed on the olives collected from Ouazzane and Sidi Kacem regions (North and North West of Morocco). Microscopic observations, based on morphological criteria, identified some of them belonging to the genus Colletotrichum. However, high morphological sim...

The attempts of argan tree (Agrania spinosa (L.) Skeels) reforestation programs, as a multi-use agro-forestry species deal with the mastering the production of superior quality seedlings in nurseries. Trichoderma spp. are well known as soil inhabitant and endophytic plant symbionts that are widely used as seed treatments to enhance plant growth. In...

The treatment of Citrus aurantium L. seeds with an endomycorrhizal consortium, originally from the rhizosphere of different species of citrus allowed roots mycorrhization and improvement of all agronomic parameters of the plants resulting from the germination of these seeds following coated seeds germination compared to those of the plants develope...

To ensure sustainable management of limiting soil P and minimize the extensive use of mineral fertilizers, friendly environmental agricultural practices are required. In this study, the effects of phospho-compost and phosphate sludges either alone or combined with an endomycorrhizal inoculum were tested on the growth parameters of maize plants. Gre...

The attempts of argan tree (Agrania spinosa (L.) Skeels) reforestation programs, as a multi-use agro-forestry species deal with the mastering the production of superior quality seedlings in nurseries. Trichoderma spp. are well known as soil inhabitant and endophytic plant symbionts that are widely used as seed treatments to enhance plant growth. In...

The objective of this study is to demonstrate the effect of different doses of phospho-compost (2.5%; 5%; 7.5%; 10%) on the growth and development of the bean plants and to see the ability of endomycorrhizal fungi contained in sludge, an essential component of this phospho-compost, to mycorrhize these seedlings. The obtained results showed that the...

Composite endomycorrhizal inocula originating from plots of saffron crops of different durations of exploitation (2, 4, 6 and 10 years) were used to inoculate substrates intended to receive saffron corms (bulbs). Four weeks after inoculation, the length of the aerial part (27 cm), the fresh weight of the aerial part (1.36 g), the fresh weight of th...

The pathogenicity of Rhizoctonia solani isolates derived from strawberry plants was evaluated against ten crops mostly grown into or closely to strawberry plantation areas plots. 13 weeks after inoculation, strawberry plants showed symptoms when they were inoculated by making collar injury using wooden toothpick whereas those inoculated by using ro...

Surveys carried out in wheat (hard and soft) and barley fields in April-May 2017 in northwestern Morocco revealed the presence of root rot symptoms in all prospected wheat and barley plots. The detailed examination of the plants brought back to the laboratory (wheat and barley) revealed the presence of necrotic lesions or black lesions on the roots...

The effect of a composite endomycorrhizal inoculum is tested on the occurrence of Fusarium wilt, caused by Fusarium solani, in chickpea plants. Eight weeks after inoculation, the aerial part’s length (24 cm), aerial part’s fresh weight (4.5 g), fresh root weight (5.54 g), leaves’ number (22) and the number of pods (4) are higher than those found in...

Surveys were carried out in wheat (durum wheat and soft wheat) and barley fields in April-May 2017 in northwestern Morocco allowed the isolation of a species of the Curvularia genus from the characteristic necrotic lesions of the root rot of wheat and barley. This fungus was isolated either in the presence of Bipolaris sorokiniana or among a variab...

The number of infectious propagules of arbuscular mycorrhizal fungi (AMF) in phosphate sludge was estimated using the most probable number (MPN) method, and compared with the results obtained by wet sieving techniques and the technique of staining roots. In the sludge soils of phosphates D, C, E, Bassin F and the rhizosphere soils of olive trees, a...

This work aims to demonstrate the pathogenicity of 37 isolates of Fusariumspecies and 4 isolates of Bipolaris sorokinianaon durum wheat (Karim and Amjad varieties), soft wheat (Wafia and Amal varieties) and barley (Hordeum pusillumand Hordeum escourgeon).All isolates induced root necrosis in inoculated wheat and barley plants. Isolates F10 (F. sola...

The present study was undertaken to estimate in vitro the capacity of twenty-three isolates of Trichoderma, isolated from soil and roots collected from adjacent sites to the Khouribga and Benguerir Phosphate mines (Morocco), and seven isolates from the laboratory's mycoteca to solubilize the Tricalcium phosphate. All these isolates showed, after th...

The diversity of endomycorrhizal fungi in the rhizosphere of Crocus sativus has been studied at five sites in the Taliouine region (Tinfat), located in Taroudant Province (Morocco), according to the number of years of soil exploitation by Saffron cultivation. In all sites, the roots of Crocus sativus carry structures of endomycorrhizal fungi. Root...

Objective: The objective of this study is to know the diversity of arbuscular mycorrhizal fungi in the soil sites adjacent to Khouribga phosphate mines (Morocco) Methods: Soil samples were collected from two localities, Hattaneand Merah Lahrach, in Khouribga Region (Morocco) nearby two operated sites of phosphates extraction. Five samples were rand...

The diversity of endomycorrhizal fungi in the rhizosphere of Crocus sativus has been studied at five sites in the Taliouine region (Tinfat), located in Taroudant Province (Morocco), according to the number of years of soil exploitation by Saffron cultivation. In all sites, the roots of Crocus sativus carry structures of endomycorrhizal fungi. Root...

The argan seed coating with a composite inoculum of arbuscular mycorrhizal fungi (AMF), originating from the rhizosphere of argan trees grown in different regions of Morocco, allowed the progressive establishment of mycorrhizal symbiosis with roots of plants from coated seeds and led a good plant development. Thus, 16 months after planting, develop...

Surveys were carried out in wheat (durum wheat and soft wheat) and barley fields in April-May 2017 in northwestern Morocco allowed the isolation of a species of the Curvularia genus from the characteristic necrotic lesions of the root rot of wheat and barley. This fungus was isolated either in the presence of Bipolaris sorokiniana or among a variab...

Arbuscular mycorrhizal fungi to have been sought in the sludge of phosphates and various plant species of the region of Khouribga (Morocco). The density of spores of mycorrhizal fungi in phosphates sludge is low, ranging from 43 and 4 spores / 100 g soil. Some species are specific, case of Glomus aureum encountered at the level of the 1.5 basin; wh...

Eighty-five species were isolated from the rhizosphere of leek plants after five months of inoculation with a composite endomycorrhizal inoculum originating from the olive tree. Analysis of the endomycorrhizal fungi spore communities that were found in the rhizosphere of leek showed the predominance of the genus Glomus with an occurrence frequency...

The endomycorrhizal fungi diversity in the rhizosphere of chickpea ( Cicer arietinum L.) and the evaluation of root mycorrhizal level were studied in six regions of Morocco: Tahla, Sefrou, Souk Larbae, Souk Tlat, Ouazzane and Jarf Melha. All chickpea roots are carrying endomycorrhizal structures. Root mycorrhizal parameters varied from one site to...

The evolution of anthracnose symptoms on the aerial part (leaves, stems and strawberries) of three varieties Fortuna, Camarosa and Festival of strawberry plants inoculated with the conidial suspensions of Colletotrichum acutatum and Colletotrichum gloeosporioides isolates was followed. The severity index and infection coefficients increased in func...

Endomycorrhizal inoculum, consisting of more than 26 mycorrhizal species, has been followed over time at the rhizosphere of olive trees. The evolution during time of this endomycorrhizal inoculum at the rhizosphere of plants has been discussed in this study. After 42 months, 45 species were isolated from the rhizosphere of inoculated olive plants....

The cultivation of the olive tree has several problems related to pests and diseases that can cause heavy economic losses by causing the death of trees. Fusarium solani was detected in the olive trees of Sidi Taibi’s nurseries located in the national road between Kénitra and Rabat in spring 2012 and 2013 (1 to 2% of diseased plants). Koch’s postula...

In spring of 2012, olive-trees with crown dieback, root rot and defoliation were observed in two years old olive tree in commercial plantations of tree nurseries in Sidi Taibi and in twenty to fifty years old field trees in Souk El Arbaa olive crops in Northwest of Morocco (Gharb area). The objective of this study was to isolate the responsible pat...

This study was carried out in a nursery to evaluate the impact of mycorrhizal fungi on the cutting's root growth, and root colonization of a Moroccan olive variety 'Picholine Marocaine' under greenhouse conditions during 2 years of cultivation. The results revealed that the inoculation with a composite inoculum of arbuscular mycorrhizal fungi (AMF)...

The identification of the arbuscular mycorrhizal fungi and the evaluation of the mycorrhization level in the roots were performed basing on the isolated spores from the soil and roots samples taken from the rhizosphere of four sugar cane parcels in the region of Dar Gueddari (North western of Morocco). The analysis of the results has shown the pres...

Inoculation with endomycorrhizae had a significant effect on the cork oak (Quercus suber) seedlings growth. The average length (55.37 cm) and weight (12.6 g) of the aerial part, the length (41 cm) and weight (26.41 g) of the root system, the rod diameter (0.56 cm) and the leaves number (120) of the plants inoculated with mycorrhizae AM were higher...

Anthracnose is an important disease affecting the yield and quality of olive production (olives, olive oil). Symptoms of anthracnose, circular soft decayed spots were observed on olives in the region of Ouazzane (North of Morocco) and Sefrou (Middle Atlas). The isolation of fungi from these olives has shown the presence of several fungal species, c...

The diversity of arbuscular mycorrhizal fungi (AMF) was examined in the cut and filled slopes of Taza region (North-East of Morocco). The identification of the spores isolated from these two types of slopes has revealed 29 species belonging to 7 genera: Glomus (14 species), Acaulospora (6 species), Gigaspora (2 species), Pacispora (3 species), Entr...

The work presented here aims to establish, from the bibliography, inventory of endomycorrhizal species reported in the rhizosphere of the date palm. 89 fungal species have been reported across the world (Egypt, Oman Soltanat, Soutern Arabia, India, London, and the Arabian Peninsula). Morocco is represented by 29 species, reported in the regions of...

Ten formulations belonging to 8 chemical families approved on Strawberry plants in Morocco were tested in vitro and in vivo. Cyprodinil + fludioxonil induced a significant inhibition of mycelial growth of the Botrytis cinerea and Pestalotia longisetula isolates reaching 90.5% at low dose (93.7 ppm). The inhibition percentages of Bartalinia laurina...