Mohamed Naguib Mohamed Abdelghany

Cairo University | CU · Department of Botany and microbiology

This study compared microbiological and chemical methods used in astaxanthin extraction from the exoskeleton of the shrimp species Penaeus japonicus and Penaeus semisulcatus. The microbiological method was performed using Saccharomyces cerevisiae (bakery yeast) or Lactobacillus acidophilus (from yogurt), followed by solvent extraction with hexane a...

Astaxanthin has been extracted in the current study using ecofriendly methods. Compared to the chemical method, a natural method using flaxseed oil at different time intervals, a biological method using standard fungal and bacterial probiotics (the fungal probiotics are Saccharomyces cerevisiae and Candida utilis, while the bacterial probiotics are...

This work aims at isolating a fungal source for l-asparaginase production to be applied in reducing acrylamide levels in coffee beans at non-cytotoxic levels. An l-asparaginase-producing fungus was isolated from an agricultural soil sample and identified as Penicillium crustosum NMKA 511. A maximum l-asparaginase activity of 19.10 U/mL was obtained...

There is strong correlation between changes in abundance of specific bacterial species and several diseases including schistosomiasis. Several studies have described therapeutic effects of curcumin (CUR) which may arise from its regulative effects on intestinal microbiota. Thus, we examined the impact of CUR on the diversity of intestinal microbiot...

Primary immunodeficiency diseases (PID), encompass a heterogeneous group of diseases, with increased susceptibility to recurrent, severe infections. Invasive fungal infections raise a serious concern related to their morbidity and mortality. Herein, we describe various fungal infections among different PID patients. Twenty-eight PID patients diagno...

Laccases are oxidoreductases enzymes that belong to blue multi-copper oxidases. In the recent years, laccases have gained application in the field of textile, pulp and paper, and food industries. In this work, optimization of cultural conditions of the fungal species Aspergillus niger was performed by Taguchi orthogonal array statistical method to...

Extracellular tyrosinase extracted from Saccharomyces cerevisiae was purified, characterized and assayed as medicinal agent. The crude enzyme activity was 7.99 U/ml and specific activity was 0.84 U/mg. The complete purification protocol was done. The final result showed one peak of pure tyrosinase with activity of 1.08 U/ml, specific activity of 2....

The cell-free culture filtrate (CFF) of the fungi Fusarium chlamydosporum NG30 and Penicillium chrysogenum NG85 was tested to synthesize silver nanoparticles (AgNPs). The synthesized AgNPs were further characterized by means of transmission electron microscopy (TEM), dynamic light scattering (DLS) and Fourier transform infra-red (FTIR) spectroscopy...

Milk clotting enzymes (MCE) produced by fungi have an important application in food and drug technology. In this study, the edible mushroom Pleurotus albidus was used as safe and efficient source of MCE. The produced MCE was purified by ammonium sulfate precipitation, dialysis, DEAE-Cellulose and Sephadex G-100 column chromatography. The full homog...

The purified laccase of Aspergillus flavus NG85 isolated from Saint Catherine Protectorate, showed a molecular weight of 68.5 kDa. Its optimum activity obtained at enzyme concentration of 0.15 mg, substrate concentration of 10 mg/ml, temperature of 47.5 oC, pH of 5 and Km of 9.09 mg/ml. The enzyme retained 85% of its activity after 4 hours of incub...

THE MICROFLORAL pictures of Saint Catherine protectorate, Giza Zoo Garden and Cairo University soil were studied. The obtained 31 microbial isolates were qualitatively and quantitatively screened for laccase production. Aspergillus flavus from Saint Catherine protectorate achieved highest laccase production on both solid and liquid media. Identific...

In our search for new fungal isolates capable of degrading polycyclic aromatic hydrocarbons (PAHs) in soil, twenty one fungal isolates were recovered from Orman Garden, Wadi Degla Protectorate and benzene station soils. All tested fungi exhibited lignin peroxidase and manganese peroxidase activities in solid as well as in liquid cultures. However,...