Moahmed M.M. Kandiel

Benha University · Department of Theriogenology

The scenario of the fertile spermatozoa with high fertilizing capability is basically dependent on gene expression‐based epididymal function. The current investigation aimed to declare the varied expression of different candidate genes ( PLA2G4D , LCN15 , CLUAP1 , SPP1 , AQP12B , DEFB110 and ESR1 ) relevant to spermatozoa features between the diffe...

Inherited disorders are one of the reasons of infertility and economic losses for the equine industry. The detection rate of chromosomal abnormalities is rising due to the use of sex chromosome linked molecular markers. Here, a rare sterile five-year-old Arabian mare with XY male chromosomes is presented. The phenotype was corresponded to female wi...

Influence of IBR vaccination timing on ovarian and uterine statuses and circulating progesterone and estradiol 17β in synchronized crossbreed heifers

Gentamicin induces gonadotoxicity in animal models, accompanied by oxidative stress. This study evaluated the histopathological protective effect of alpha-lipoic acid against gentamicin-induced gonadotoxicity. A parallel experimental study included 50 albino Wister rats, which were divided into 5 groups of ten according to the intraperitoneal inter...

Gentamicin induces gonadotoxicity in animal models, accompanied by oxidative stress. This study evaluated the histopathological protective effect of alpha-lipoic acid against gentamicin-induced gonadotoxicity. A parallel experimental study included 50 albino Wister rats, which were divided into 5 groups of ten according to the intraperitoneal inter...

Studying testicular genes´ expression may give key insights into precise regulation of its functions that influence epididymal sperm quality. The current study aimed to investigate the abundance of candidate genes involved in the regulation of testicular functions specially those regulate sperm function (PLA2G4D, SPP1 and CLUAP1), testicular steroi...

Because there is a growing demand for use of ultrasonography in studying, diagnosis, and monitoring many physiological events and diseases of animals, the goal of this special edition Research Topic is to shed light on the progress made in the past decade in applications of diagnostic ultrasonography in different aspects of physiological and pathol...

This work aimed to determine the effect of cysteamine (25, 50, 100 and 200 μM) incorporated during dilution on frozen thawed buffalo semen quality. Semen was collected twice weekly for 7 consecutive weeks from three Egyptian buffalo bulls using an artificial vagina. Semen samples were pooled and extended with a Tris-based extender, cooled, equilibr...

Acute kidney injury is a common problem in dogs and is associated with significant morbidity and mortality. So, the present study aimed to evaluate symmetric dimethylarginine (SDMA) and Doppler ultrasonography including resistive index (RI) in the diagnosis of acute kidney injury in dogs. Ten healthy mongrel dogs were injected with gentamicin sulfa...

This study aimed at evaluating the litter size influence on fetal growth (marked by biparietal diameter), steroid hormones (estradiol and progesterone), oxidative stress markers [Total antioxidant capacity (TAC), superoxide dismutase (SOD), catalase (CAT), Glutathione peroxidase (GPx), and malondialdehyde (MDA)], total proteins, and serum-free RNA....

T HE present study aimed to clarify the alternations in alpha-fetoprotein (AFP), phosphorus (Ph), calcium (Ca), and metabolic hormones (Growth hormones (GH), insulin, and thyroid gland hormones) in singleton, twin, and triplet pregnancy in goats. Animals were examined for pregnancy ultrasonographically during mid-pregnancy (6-14 weeks of gestation)...

This study was conducted in two experiments. Experiment 1 aimed to investigate the growth promoting and anti-oxidative effects of Azolla nilotica (AZN) and Spirulina platensis (SP) in Oreochromis niloticus. Seven fish groups (G1-G7), each in three replicates, were fed a basal diet (control), AZN 5%, AZN 10%, SP 0.5%, SP 1%, a mixture of Azolla nilo...

T HIS study aimed to (1) identify the status of reproductive tract of repeat-breeder heifers (RBH) ultrasonography, as well as (2) determinethe variations in steroid hormones, oxidative stress biomarkers, and cell-free RNA in serum.These culled animals from400 heifers at the breeding stationwere examined ultrasonography, and whole blood samples wer...

Acute kidney injury is a common problem in dogs and is associated with significant morbidity and mortality, so this study was aimed to evaluate Symmetric dimethylarginine (SDMA), Doppler ultrasonography including resistive index (RI) in the diagnosis of acute kidney injury in dogs. Ten healthy mongrel dogs were injected with gentamicin sulphate 10%...

The aim of the present study was to buildup equation (s) for an intra-uterine fetal aging in Egyptian local sheep and she-goats breed using ultrasound. This study was carried out on a total number of 110 Baladi sheep and 74 she-goats under the field condition of Qalyubia governorate, Egypt. Ultrasound examination was conducted through trans-rectal...

Keywords The aim of the present study was to buildup equation (s) for an intra-uterine fetal aging in Egyptian local sheep and goats breed using ultrasound. This study was carried out on a total number of 110 Baladi sheep and 74 goats under the field condition of Qalyubia governorate, Egypt. Ultrasound examination was conducted through trans-rectal...

Keywords The current study entails the data obtained from ultrasonography investigation of the reproductive statuses of ewe (n=232) and does (n=164) admitted to Meet Kenana, Veterinary Clinic, Tukh, Qalyubia governorate aiming to declare the contributing factors (species, season, body weight and age) impact their fertility. Pregnancy examination re...

Buffalo spermatozoa are more sensitive for cryopreservation compared to other species. This study aimed to evaluate the consequences of quercetin against cryodamage of buffalo frozen–thawed spermatozoa characteristics. Semen of Egyptian bulls (n = 4) was extended in OptiXcell extender incorporated with quercetin at 0 (control), 2.5, 5.0, 10.0, 20.0...

This study aimed to evaluate the use of glycerolized bovine pericardium (GBP) compared to polypropylene mesh (PPM) in repairing of large abdominal wall defects in animal model, and to investigate the role of platelet rich fibrin (PRF) in promoting this repair. Fresh bovine pericardium collected from local abattoir were processed and preserved in 99...

Introduction: Reconstruction of Abdominal Wall Defects relied over years on using different Prosthetic implants as Polypropylene Mesh (PPM), Polyester, Dacron and expanded Polytetrafluoroethylene (ePTFE). Using such biomaterials becomes facing several drawbacks as they are non-bioabsorbable, therefore they remain inside the body as permanent foreig...

AB S T RA C T Lactoferrin (LF), an iron-chelating 80-kDa glycoprotein closely related in structure to iron transport protein transferrin, known as red protein. LF gene is highly conserved among various species and considered as one of the candidate genes for endometritis and mastitis resistance. This study aimed to detect single-nucleotide polymorp...

Background: Drug-induced reproductive organs toxicities is an important aetiology in investigation of male infertility. The aim is to study levofloxacin effect on male reproductive system in comparison to ciprofloxacin.Methods: Twenty-five male wister rats weighted 230±20 gm and aged 8 weeks were randomly divided into five groups of five. The first...

Background: Many drugs are implicated in male infertility and screening for medication history is an important for diagnosis and treatment of the problem. The aim is to study amikacin effect on male reproductive system in comparison to gentamicin.Methods: Twenty-five male wister rats weighted 220±20 gm and aged 8 weeks were randomly divided into fi...

Objective: To evaluate the cryoprotective effect of butylated hydroxytoluene on buck frozen semen. Methods: Semen was collected from Boer (n=6) and Zaraibi (n=6) bucks by electroejaculator for 5 weeks. Semen aliquots were diluted at 38 °C in Tris-buffer with egg yolk 15.0% (vol/vol) (Tris- egg yolk extender) or soya lecithin 2.5% (weight/vol) (Tris...

AB S T RA C T Lactoferrin (LF), an iron-chelating 80-kDa glycoprotein closely related in structure to iron transport protein transferrin, known as red protein. LF gene is highly conserved among various species and considered as one of the candidate genes for endometritis and mastitis resistance. This study aimed to detect single-nucleotide polymorp...

Leptin gene is one of the potential genes involves intricately in metabolism and growth of animals. Leptin has myriad effect on tissues and endocrine system that ultimately lead to the whole body energy metabolism and has major impact on performance and wellbeing of livestock species. Owing to have key biological features, leptin is one of themost...

This study aimed at investigating the efficacy of two estrus synchronization protocols on follicular changes and hemodynamics. Pluriparous Egyptian buffaloes (n = 36) were synchronized either with CIDR-PGF2α (7-days CIDR insert with PGF2α injected on the 6th day; n = 18) or Ovsynch-CIDR (Ovsynch protocol concurrent with 7-days CIDR insert; n = 18)....

The objective of the current study was to evaluate the accuracy of trans-rectal color Doppler ultrasonography for early diagnosis of pregnancy in Egyptian buffalo based on subjective assessment of luteal blood flow (LBF). After timed artificial insemination (TAI), a total of 112 pluriparous buffalo were subjectively evaluated for LBF and there were...

Original Research Article Enormous damage to spermatozoa occurs during handling and/or deep cold storage. In the meantime, detection of the alternations of spermatozoa in routine semen analysis is very subjective and depends upon the skill of the technician in the lab. This work aimed to verify the value of electron microscopic as well as single ce...

This study aimed to evaluate the use of glycerolized bovine pericardium (GBP) compared to polypropylene mesh (PPM) in repairing of large abdominal wall defects in animal model, and to investigate the role of platelet rich fibrin (PRF) in promoting this repair. Fresh bovine pericardium collected from local abattoir were processed and preserved in 99...

This study aimed to assess the relation between the morphometric and histological attributes of the epididymis, and epididymal semen features in mature dromedary camels. The testes with the attached epididymis (n=50) were collected from adult camels along the rutting season from December to April. The separated epididymis was evaluated for weight a...

This work was planned to study the effect of dog breeds on the physical and chemical properties of semen. A total number of 53 dogs of Caucasian (n=3), German shepherd (n=37), Malinois (n=8) and Rottweiler (n=5) breeds and mixed ages were used for semen collection along a year from 2016 to 2017. Semen was collected using an artificial vagina and ex...

Objective: To assess effect of buffalo bull breed on the development and cryotolerence of the in vitro produced embryos. Methods: Three types of frozen semen were adopted; Egyptian, Italian and cross-bred (Egyptian-Italian) breeds were used for in-vitro fertilization and vitrification of their embryos. Oocytes were collected from buffalo ovaries an...

This study aimed to assess the relation between the morphometric and histological attributes of the epididymis, and epididymal semen features in mature dromedary camels. The testes with the attached epididymis (n=50) were collected from adult camels along the rutting season from December to April. The separated epididymis was evaluated for weight a...

Egypt is anticipated to be potentially influenced by the global climate warming. Therefore, the current study aimed to evaluate the influence of age on the fertility potential of Arabian stallions during summer breeding months. Arabian horses grouped according to their age into three groups, each involved six stallions: young (5-6 years), middle (1...

Production of high quality embryos in vitro needs an efficient in vitro fertilization (IVF). Seminal origin is one of the important factors that affects the success of in vitro embryo production. So our goal was to determine the effect of using fresh and frozen semen in fertilization on developmental competence and cryo-survival of buffalo embryos....

This work was planned to study the effect of dog breeds on the physical and chemical properties of semen. A total number of 53 mixed-age dogs of Caucasian (n=3), German shepherd (n=37), Malinois (n=8) and Rottweiler (n=5) breeds were used for semen collection using an artificial vagina. Semen was examined for color, density, volume, pH, sperm indiv...

Production of high quality embryos in vitro needs an efficient in vitro fertilization (IVF). Seminal origin is one of the important factors that affects the success of in vitro embryo production. So our goal was to determine the effect of using fresh and frozen semen in fertilization on developmental competence and cryo-survival of buffalo embryos....

Bone morphogenetic protein 15 (BMP15/FecX) gene is considered one of the major genes and a candidate marker for the reproduction in farm animals, especially sheep. The present study aimed to detect the genetic polymorphisms of BMP15 gene in sheep using PCR-RFLP technique. In the present study, 115 ewes were assigned into high and low prolificacy ca...

Various methods are being employed to detect early pregnancy in domestic animals. This study aimed to predict early pregnancy in buffaloes via measuring the corpus luteum (CL) diameter, the luteal blood flow (LBF) area, the uterine blood flow (UBF) vascularization area, and progesterones in saliva and serum for non-pregnant (NPBs, N = 12) and pregn...

Production of high quality embryos in vitro needs an efficient in vitro fertilization (IVF). Seminal origin is one of the important factors that affects the success of in vitro embryo production. So our goal was to determine the effect of using fresh and frozen semen in fertilization on developmental competence and cryo-survival of buffalo embryos....

To elucidate the adverse effects of azithromycin (member of macrolides) or ceftiofur sodium (third generation of cephalosporins) on male fertility, the male reproductive organs, testosterone, spermiogram and intra-testicular oxidative stress markers have been monitored in adults’ male albino rats (200-250 b.wt.) after oral azithromycin or subcutane...

The current study aimed to screen the growth and viability of embryo from Day 21 to Day 56 post-breeding in Egyptian buffaloes. Twenty buffaloes were synchronized and examined serially by means of real-time B-mode ultrasound equipped with 12 MHz endorectal transducer. The developing embryonic sac diameter (ESD), crown rump length (CRL), head diamet...

Eighty animals (21 cow calves, 17 buffalo calves, 19 sheep, 6 goats, 5 bulls, 6 horses, 5 donkeys and one mule) with umbilical, abdominal, inguinal or scrotal hernias were included in this study. Animals were arbitrarily allotted to one of two groups. In the first group, the hernia defects were repaired by using Glycerolized Bovine Pericardium (GBP...

A description of normal ultrasonographic alternations of the uterus in the postpartum (PP) in buffaloes is essential for the diagnosis of pathological conditions and improves the ability to differentiate puerperal pathology from normal physiological changes. The current study aimed to characterize the changes in uterine features assessed ultrasonog...

Prediction of reproductive status by a simple, non-invasive and applicable procedure is greatly desirable. Saliva sampling is a simple and low-cost process. The purpose of this study was to assess the value of saliva crystallization patterns (SCP) for the prediction of pregnancy in buffalo cows. A total number of 160 saliva samples were collected f...

Objective: To study genetic variation in buffalo follicle stimulating hormone beta-subunit (FSHB) gene and its association with fertility. Methods: In this experimental study, blood samples were collected by standard methods using EDTA anticoagulant and transrectal ultrasound examination was conducted on fertile (n=74) and infertile buffaloes with...

Prediction of reproductive status by a simple, non-invasive and applicable procedure is greatly desirable. Saliva sampling is a simple and low-cost process. The purpose of this study was to assess the value of saliva crystallization patterns (SCP) for the prediction of pregnancy in buffalo cows. A total number of 160 saliva samples were collected f...

This study aimed to evaluate the numerous azithromycin (as a member of macrolides) effects on the male reproductive organs, spermiogram, testicular oxidative stress markers of adults’ male albino rats. Azithromycin was administered orally once daily to male rats (200-250 b.wt.) at a dose of 45 mg (therapeutic) or 90 mg/kg b.wt. (double-therapeutic)...

This study aimed to evaluate the numerous azithromycin (as a member of macrolides) effects on the male reproductive organs, spermio-gram, testicular oxidative stress markers of adults’ male albino rats. Azithromycin was administered orally once daily to male rats (200-250 b.wt.) at a dose of 45 mg (therapeutic) or 90 mg/kg b.wt. (double-therapeutic...

The current study was achieved to designate the accessory sex glands, ampullae and urethra of 25 healthy male donkeys using the ultrasonography. Animals were classified according to their age into young peri-mature (i.e. ≤2 years; n=7) and old mature (>3 years; n=18). The examination was done per rectum by using Magic 2200 scanner with a 6 MHz line...

This study was conducted to clarify the implications arising from the use of ceftiofur sodium, third generation of cephalosporins, on male reproductive organs, testosterone hormone and testicular oxidative stress markers. Adult male rats (200-250 g) were daily subcutaneously injected with ceftiofur sodium at a dose of 7.2 mg or 14.4 mg/kg b.wt. for...

Objective: To assess the effects of epidermal growth factor (EGF) on the Egyptian buffalo bull frozen semen, EGF was incorporated at 0 (control), 50, 100, 200 and 400 ng/mL of extender (Bioxcell®). Methods: Semen features, spermatozoa biometry, total liberated amounts of enzymes (aspartate transaminase, alanine aminotransferase, lactate dehydrogena...

This study testified the effect of honey supplementation (0.5-4.0%) in milk on the quality of chilled and frozen buffalo spermatozoa. Semen was chilled with/without honey and examined for motility, viability, plasma membranes integrity by hypo-osmotic swelling test (HOS) at 0, 1, 2 and 4 h. Frozen-thawed semen was examined for the same criteria bes...

The present investigation aimed to outline the effects of PNP on Amphiregulin (AREG)-induced oocytes maturation. Bovine COCs were aspirated from small antral follicles (2–6 mm in diameter) and incubated in IVM medium contained 1 µl /ml Amphiregulin of human origin supplemented with PNP at 0 (control), 10 7 M (LD), 10 M (MD), 10 M (HD) and 10 M (VHD...

The aims of the present study were to assess the effects of cysteamine as an anti-oxidant on the rate of in vitro maturation (IVM) of buffalo oocytes (experiment 1), and their viability and nuclear status following vitrification (experiment 2). Immature oocytes with compact cumulus cells obtained from the ovaries of slaughtered animals were harvest...

This work aimed to search for single nucleotide polymorphisms of gonadotropin releasing hormone receptor (GnRHR) gene in Egyptian buffalo which involved in endocrine control of fertility. An ultrasonographic examinations of anestrum and repeat breeders’ buffaloes were performed prior to blood sampling through a transrectal ultrasonography. DNA was...

Ovulation can be regulated by a group of genes, termed as fecundity (Fec) genes. The aim of this study is to identify genetic polymorphism in the Booroola (FecB) gene in Barki, Rahmani, and Ossimi sheep breeds with different physiological status diagnosed by ultrasound. Accordingly, animals were early classified into three groups: carrying single f...