Mitsuaki Nishibuchi

Kyoto University | Kyodai · Center for Southeast Asian Studies

Escherichia coli is a commensal bacterium that lives in human and animal intestines. Shiga toxin-producing strains of E. coli STECs are responsible for most food-related E. coli infections. Pathogenic E. coli transmits to human bodies due to the consumption of contaminated, raw, or undercooked food. is study was conducted to identify the prevalence...

[This corrects the article DOI: 10.3389/fmicb.2020.537889.].

Vibrio parahaemolyticus is a foodborne bacterial pathogen that may cause gastroenteritis in humans through the consumption of seafood contaminated with this microorganism. The emergence of antimicrobial and multidrug-resistant bacteria is another serious public health threat worldwide. In this study, the prevalence and antibiotic susceptibility tes...

Bacillus cereus spores are capable of surviving the harsh environment and more often, they cause great concern to the dairy industry. The current research was conducted to study the effect of temperature on germination and growth of B. cereus spores in UHT chocolate milk; the study was carried out at 8°C, 25°C and 35°C over a span of seven days. Th...

A preliminary quantitative microbial risk assessment of Vibrio parahaemolyticus in short mackerels was conducted from retail to consumption levels in a stochastic model. The key uncertainties included of growth during retail display, transportation duration, and microbial log reduction after the preparation and cooking steps were taken into conside...

Background Vibrio parahaemolyticus is a causative agent of gastroenteritis. Most of the clinical isolates carry either tdh and/or trh genes which are considered as the major virulence genes of this pathogen. In this study, the clinical isolates of V. parahaemolyticus carrying trh gene (n = 73) obtained from 1886 to 2012 from various countries were...

Pandemic O3:K6 Vibrio parahaemolyticus emerged in 1996. Since then, this strain of pathogen and its serovariants (predominantly O1:KUT [untypable], O1:K25 and O4:K68) have caused gastroenteritis worldwide. Owing to the limitation in established K antisera, tracking the sources of KUT for epidemiological investigation is difficult. Therefore, the ef...

Vibrio vulnificus is a Gram-negative, halophilic bacterium that mainly inhabits marine environments. It is responsible for causing gastroenteritis upon consuming contaminated seafood or exposure of an open wound to seawater. In addition, it has the ability to cause wound infection and septicaemia. It is also known to be an opportunistic organism th...

Kitchen mishandling practices contribute to a large number of foodborne illnesses. In this study, the transfer and cross-contamination potential of Vibrio parahaemolyticus from bloody clams to ready-to-eat food (lettuce) was assessed. Three scenarios were investigated: 1) direct cross-contamination, the transfer of V. parahaemolyticus from bloody c...

Silver nanoparticles (AgNPs) used in this study were synthesized using pu-erh tea leaves extract with particle size of 4.06 nm. The antibacterial activity of green synthesized AgNPs against a diverse range of Gram-negative foodborne pathogens was determined using disk diffusion method, resazurin microtitre-plate assay (minimum inhibitory concentrat...

This goal of this study was to investigate the presence of Vibrio cholerae in street food, namely satar and otak-otak, using Loop-Mediated Isothermal Amplification (LAMP), multiplex Polymerase Chain Reaction (mPCR) and conventional plating on Thiosulphate Citrate Bile-Salt Sucrose (TCBS) agar methods. A total of 78 satar and 35 otak-otak were purch...

Methicillin-resistant Staphylococcus aureus (MRSA) is a remarkable pathogen which causes nosocomial diseases worldwide. In this study, raw green mussel and cockle samples in Malaysia were collected and investigated for the occurrence of MRSA. A total of 80 samples were analyzed using phenotypic and molecular approaches. A assembling of the most pro...

Campylobacter is a major foodborne pathogen frequently associated with human bacterial gastroenteritis in the world. This study was conducted to determine the prevalence and antibiotic resistance of Campylobacter spp. in the beef food system in Malaysia. A total of 340 samples consisting of cattle feces (n = 100), beef (n = 120) from wet markets an...

Given the remarkable increase of public interest in organic food products, it is indeed critical to evaluate the microbiological risk associated with consumption of fresh organic produce. Organic farming practices including the use of animal manures may increase the risk of microbiological contamination as manure can act as a vehicle for transmissi...

Numerous prevalence studies and outbreaks of Vibrio parahaemolyticus infection have been extensively reported in shellfish and crustaceans. Information on the quantitative detection of V. parahaemolyticus in finfish species is limited. In this study, short mackerels (Rastrelliger brachysoma) obtained from different retail marketplaces were monitore...

Vibrio parahaemolyticus is a Gram-negative, halophilic bacterium isolated from marine environments globally. After the consumption of contaminated seafood, V. parahaemolyticus causes acute gastroenteritis. To initiate infection, a wide range of virulence factors are required. A complex group of genes is known to participate in the pathogenicity of...

Cross contamination is one of the most important contributing factors in foodborne illness originating in household environments. The objective of this research was to determine the transfer between naturally contaminated chicken liver and leg to cutting board, hand glove, knife and cucumber, during slicing. The microorganism tested was Campylobact...

modified crops which brought up a controversy on the safety usage of genetically modified organisms (GMOs). It has been implemented globally that all GMO products and its derived ingredients should have regulations on the usage and labelling. Thus, it is necessary to develop methods that allow rapid screening of GMO products to comply with the regu...

To date, cholera has cycle the world seven times through the seven pandemic cycles that has affected tens of millions of people. The objective of this study was to determine the presence and density as well as the antibiotic resistance profile of Vibrio cholerae isolated from catfish (Pangasius hypohthalamus). From the combination of the Most Proba...

Foodborne pathogens have become a constant threat to the consumer and food industry. Reduce efficacy of antibiotics with emergence of resistant bacteria has limited the opportunities for controlling pathogenic bacteria in food commodities and treating foodborne infections. Bacteriophages can be a promising alternative for alleviate the risk of tran...

Campylobacter is globally recognized as a major cause of foodborne infection in humans, whilst the development of antimicrobial resistance and the possibility of repelling therapy increase the threat to public health. Poultry is the most frequent source of Campylobacter infection in humans, and southeast Asia is a global leader in poultry productio...

Vibrio parahaemolyticus is a halophilic Gram-negative bacterium that is considered among gastrointestinal pathogens. Thirty isolates were tested for their susceptibility using 14 different antibiotics. One V. parahaemolyticus isolate was resistant to 10 antibiotics (cefotaxime, ceftazidime, tetracycline, amikacin, ciprofloxacin, levofloxacin, oflox...

A preliminary risk assessment model of Vibrio parahaemolyticus in bloody clams in Malaysia was developed. The study framework was established based on the currently available experimental and survey data, in combination with the @RISK software to simulate based on Monte Carlo simulation. The model framework was categorized into two stages, the 1) e...

Salmonellosis is one of the major food-borne diseases in many countries. This study was carried out to determine the occurrence of Salmonella spp., Salmonella Enteritidis, and Salmonella Typhimurium in raw chicken meat from wet markets and hypermarkets in Selangor, as well as to determine the antibiotic susceptibility profile of S. Enteritidis and...

Vibrio parahaemolyticus is a halophilic bacterium that causes seafood-borne gastroenteritis which can occur through direct or cross-contamination. In this study, biosurfactant-producing marine bacteria were isolated from 28 soil samples collected from mangrove and coastal regions. Using the cross streak technique, 26 isolates were found to inhibit...

Vibrio parahaemolyticus is a main foodborne disease in seafood and generally seafood is easily deteriorates in quality of color and flavor. In this study, clove (Syzygium aromaticum) extract shows potent antibacterial activity against growth of antibiotics resistant Vibrio parahaemolyticus on seafood samples (cockles and shrimps). Vibrio parahaemol...

This study aimed to investigate the susceptibility pattern of Staphylococcus aureus isolated from human and environmental surfaces in a research laboratory. A total of 320 samples from nostril (n=80), hand (n=80), door knob (n=80) and table surface (n=80) were collected for 16 weeks, before and after work. A total number of 256 samples were found p...

Vibrio parahaemolyticus is an important seafood-borne pathogen. V. parahaemolyticus carrying either tdh or trh or both genes is considered a pathogenic strain. The objectives of this study were to examine the prevalence and concentration of V. parahaemolyticus in shrimp processed in a factory using most probable number (MPN) combined with either po...

Vibrio alginolyticus is a halophilic Gram-negative marine bacterium. The current study investigated the presence of the tdh and trh virulence factors in isolates of Vibrio alginolyticus. Five samples of Mantis Shrimp (Oratosquilla oratoria) were collected from local markets around Selangor, Malaysia. Samples were then examined using the MPN and mul...

During 2009 to 2010, a total of 408 blood samples collected from malaria patients in Ranong (149) and Yala (259) Provinces, Thailand were investigated for Plasmodium spp using microscopic examination. There are no statistical differences in the prevalence of P. falciparum and P. vivax in samples collected from Ranong and Yala (46% vs 52%, and 54% v...

Salmonella has been reported to be presence both in raw and processed foods worldwide. In this study, the prevalence, quantification and antibiotic susceptibility of Salmonella isolated from raw vegetables or locally known as ulam such as asiatic pennywort (Centella asiatica (L) Urb), water dropwort (Oenanthe javanica (Blume) DC), long bean (Vigna...

Vibrio parahaemolyticus is a marine microorganism that can cause seafood-borne gastroenteritis in humans. The infection can be spread and has become a pandemic through the international trade of contaminated seafood. Strains carrying the tdh gene encoding the thermostable direct hemolysin (TDH) and/or the trh gene encoding the TDH-related hemolysin...

Vibrio parahaemolyticus is a halophilic bacterium that commonly inhabits the marine and estuarine environments. This organism is also one of the leading causative pathogen of gastroenteritis often related to consumption of raw or undercooked seafood. In this study, molluscan shellfish (bloody clams and surf clams) and crustaceans (shrimps) were mon...

Not only O157 Shigatoxin-producing Escherichia coli (STEC) but some of non-O157 STECs are attracting attentions as clinically important STEC and a possible food-borne spread of these pathogens is becoming a worldwide concern. In this study, we developed a sensitive, specific, and simple method to detect STECs of clinical significance in retail beef...

Acute hepatopancreatic necrosis disease (AHPND) is a serious disease in shrimp and results in considerable losses for the shrimp aquaculture industry. The etiologic agent of AHPND has recently been identified as a unique strain of Vibrio parahaemolyticus: One that is different from human pathogenic V. parahaemolyticus strains. In this study, two se...

Escherichia coli and Escherichia coli O157 were identified from "selom" (Oenanthe stolonifera), "pegaga" (Centella asiatica), beef, chicken, lamb, buffalo, "ulam Raja" (Cosmos caudatus) and "tenggek burung" (Euodia redlevi). The bacteria were recovered using chromagenic agar. Isolated Escherichia coli and Escherichia coli 0157 were further characte...

Cross contamination of Listeria monocytogenes from two different surfaces (plastic wrappers and stainless steel coupons) was simulated using a series of different weights. Enumeration of transfer was based on surface bacterial counts of biofilm stained surfaces. Direct contact between 2 surfaces has a constant rate of transfer that is independent o...

Although thermostable direct hemolysin-producing (tdh+) Vibrio parahaemolyticus is the leading cause of seafood-borne gastroenteritis, the enumeration of tdh+ V. parahaemolyticus remains challenging due to its low densities in the environment. In this study, we developed a most-probable-number (MPN)-based procedure designated A-IS1-LAMP, in which a...

E. coli serotype 0157 is well known to cause serious illnesses in humans. However, there has been no case report to date of this serotype in Thailand. In this study, we report for the first time E. coli 0157 (designated as PSU120) isolated from a stool sample among 228 diarrheal swab samples at Hat Yai Hospital, Songkhla Province, Thailand. This PS...

Correlation between the numbers of Vibrio parahaemolyticus and its specific bacteriophages in cockles was investigated from June 2009 to May 2010 in Hat Yai, Songkhla, Thailand. Cockles obtained monthly from a local market were sampled to determine the numbers of V. parahaemolyticus and bacteriophages that could form plaques on ten strains of pande...

Vibrio parahaemolyticus (V. parahaemolyticus) is a halophilic, Gram-negative human pathogen known as a leading cause of seafood-derived food poisoning. Due to high contamination rate of seafood in Asian countries, V. parahaemolyticus is considered as a food safety concern. V. parahaemolyticus is able to produce biofilm which is more resistant towar...

Vibrio parahaemolyticus is recognized as a frequent causal agent of human gastroenteritis due to the consumption of raw, undercooked or mishandled seafood in many Asian countries. The number of V. parahaemolyticus cases reported is on the rise, and this becomes a concern to the Asian countries as seafood is favoured by Asians. This study aimed to d...

Meats are apparently found to be the vehicle of several pathogenic bacteria. Although the existence of enteropathogenic Escherichia coli (EPEC) was reported to be reserved in certain meat types in various countries around the globe, insufficient informations about EPEC were documented in Thailand. In this study, the preliminary investigation of EPE...

Pathogenic Vibrio parahaemolyticus is one of the leading causes of bacterial gastroenteritis in many countries. Among the strains examined, 36 RAPD-types were found when amplified with primers OPA8 and OPA10. The analysis shows the majority of V. parahaemolyticus isolates originated from seafood were branched into four major clusters at 18.2%, 20.7...

The bacterium, Vibrio parahaemolyticus was isolated from 776 patients at Hat Yai Hospital in Southern Thailand from 2006 to 2010. 51.3–73.6% of the isolates were tdh+ trh− and Group-specific PCR positive pandemic strains. A comparison of the number of V. parahaemolyticus isolates in this study and that from the same hospital in 2000–2005 indicates...

A total of 63 beef offal samples (beef liver = 16; beef lung = 14; beef intestine = 9; beef tripe = 15; beef spleen = 9) from three wet markets (A, B, and C) in Selangor, Malaysia were examined for the prevalence and microbial load of Listeria monocytogenes. A combination of the most probable number and polymerase chain reaction (MPN-PCR) method wa...

Occurrences of the Gram positive pathogenic Listeria monocytogenesin foods an; often found to be low in level with the possibility of being in an injured state due to the food processes. Various standard protocols have been established to enable the recovery and identification ofL. monocytogenesceUs from foods. Even though L. monocytogenes is a com...

The microbial safety of foods continues to be a main concern to consumer, regulatory agencies and food industries around the world. In this study, a total of 394 presumptive vibrios from seafood samples (n=394) consists of cockles (Anadara granosa) ,clams (Mya arenaria), (shrimp) Penaeous spp. and (squid) Loligo opalescens have been isolated. Polym...

A total of 216 chicken offal samples (chicken liver = 72; chicken heart = 72; chicken gizzard = 72) from wet markets and hypermarkets in Selangor, Malaysia, were examined for the presence and density of Listeria monocytogenes by using a combination of the most probable number and PCR method. The prevalence of L. monocytogenes in 216 chicken offal s...

Infection by Vibrio parahaemolyticus is established if seafood contaminated with pathogenic strains of this bacterium at a sufficiently high dose is consumed. We reported recently that V parahaemolyticus isolates from 67% of patients constituted a homogeneous population of a pathogenic strain and a heterogeneous population of V parahaemolyticus var...

Biofilm formation can lead to various consequences in the food processing line such as contamination and equipment breakdowns. Since formation of biofilm can occur in various conditions; this study was carried out using L. monocytogenes ATCC 19112 and its biofilm formation ability tested under various concentrations of sodium chloride and temperatu...

It is necessary to consider the kind and dose of the drug, the species and the stage of Plasmodium in the blood of the patient when anti-malaria drugs are administered to the patients. It is generally thought that the effect of treatment can be observed if a clinician put these factors into consideration. However, the number of the factors increase...

Biofilm formation can lead to various consequences in the food processing line such as contamina-tion and equipment breakdowns. Since formation of biofilm can occur in various conditions; this study was carried out using L. monocytogenes ATCC 19112 and its biofilm formation ability tested under various concentrations of sodium chloride and temperat...

Foodborne diseases are mainly caused by bacterial contamination which can lead to severe diarrhea. This study aimed to detect the presence of Shiga toxin-Producing Escherichia coli O157, Escherichia coli non-O157 and virulence gene in raw vegetables. The samples were purchased from wet market and hypermarket in Selangor. The detections were carried...

This study aims to determine the presence of extended-spectrum (ESBL) in Klebsiella pneumoniae isolated from raw vegetables by genotypic and phenotypic method. Fifty-three K. pneumoniae isolates that were obtained by plating method were confirmed by PCR. Isolates obtained were screened for their resistance to selected antibiotics. Phenotypic tests...

A total of 63 beef offal samples (beef liver = 16; beef lung = 14; beef intestine = 9; beef tripe = 15; beef spleen = 9) from three wet markets (A, B, and C) in Selangor, Malaysia were examined for the prevalence and microbial load of Listeria monocytogenes. A combination of the most probable num-ber and polymerase chain reaction (MPN-PCR) method w...

Listeria monocytogenes (L. monocytogenes) is a gram positive food-borne pathogen that is able to form biofilm on food factory surfaces. Formation of biofilm makes the bacteria much more resistance to environmental stresses such as disinfectant. The extracellular polymeric matrix (biofilm structure) which is mostly comprised of sticky extracellular...

Molecular typing methods have been widely applied for many purposes. In this study, such methods were adopted as DNA fingerprinting tools to determine the origin and divergence of virulent Vibrio parahaemolyticus strains found in local seafood. Although not all strain carry virulent tdh and trh gene, increasing prevalence demands an effective finge...

This study aimed to determine the prevalence Listeria monocytogenes in raw chicken meat samples at hypermarkets and wet markets. Chicken drumsticks, breasts, and thighs were randomly selected. The most probable number (MPN) PCR method was used to quantify the L. monocytogenes in the samples. Listeria monocytogenes was detected in 20% of the samples...

The development of the biological synthesis of nanoparticles using microorganisms or plant extracts plays an important role in the field of nanotechnology as it is environmentally friendly and does not involve any harmful chemicals. In this study, the synthesis of silver nanoparticles using the leaves extract of Chinese tea from Camellia sinensis i...

It is important to obtain the information on food security in the countries other than Japan since more than 60 % of the food consumed come from these countries. Food security is now considered as a global issue. A global trend persuading us to provide safe food to humans is based on the concept of human security development associated with a sense...

Plasmodium knowlesi, a simian malaria parasite, has been reported in humans in many Southeast Asian countries. In Thailand, most of the limited numbers of cases reported so far were from areas near neighbouring countries, including Myanmar. Blood samples collected from 171 Thai and 248 Myanmese patients attending a malaria clinic in Ranong province...

◄► Available from: Burhan Imamwerdi DownloadPlain Text Page 1 African Journal of Microbiology Research Vol. 6(2), pp. 447-452, 16 January, 2012 Available online at http://www.academicjournals.org/AJMR DOI: 10.5897/AJMR11.1436 ISSN 1996-0808 ©2012 Academic Journals Full Length Research Paper Molecular characterization of Vibrio cholerae non- O1/non-...

Background: Plasmodium knowlesi, a simian malaria parasite, has been reported in humans in many Southeast Asian countries. In Thailand, most of the limited numbers of cases reported so far were from areas near neighbouring countries, including Myanmar.