Mitchell T Ringuet

Mitchell T Ringuet
University of Melbourne | MSD · Department of Anatomy and Physiology

Masters of Biomedical Science
Investigating novel GPCR interactions

About

21
Publications
2,649
Reads
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315
Citations
Citations since 2016
18 Research Items
315 Citations
2016201720182019202020212022020406080
2016201720182019202020212022020406080
2016201720182019202020212022020406080
2016201720182019202020212022020406080
Introduction
Investigating novel heteromeric receptors: How do ghrelin receptors and dopamine receptors work together to regulate colonic motility. To answer this question I'm using drugs that target these receptors and observing responses using whole cell patch clamp and calcium imaging in spinal cord slices.
Additional affiliations
February 2018 - July 2019
The University of Queensland
Position
  • Research Assistant
Description
  • I worked in the Angonno Lab looking at the regulation of glutamate receptor trafficking by the calcium- and lipid-binding protein, copine-6

Publications

Publications (21)
Article
The growth hormone secretagogue receptor 1a (GHSR1a) is intriguing because of its potential as a therapeutic target and its diverse molecular interactions. Initial studies of the receptor focused on the potential therapeutic ability for growth hormone (GH) release to reduce wasting in aging individuals, as well as food intake regulation for treatme...
Article
Full-text available
Parkinson’s disease (PD) is associated with neuronal damage in the brain and gut. This work compares changes in the enteric nervous system (ENS) of commonly used mouse models of PD that exhibit central neuropathy and a gut phenotype. Enteric neuropathy was assessed in five mouse models: peripheral injection of MPTP; intracerebral injection of 6-OHD...
Article
Full-text available
Dietary organic selenium (Se) is commonly utilized to increase formation of selenoproteins, including the major antioxidant protein, glutathione peroxidase (GPx). Inorganic Se salts, such as sodium selenite, are also incorporated into selenoproteins, and there is evidence that nanoelemental Se added to the diet may also be effective. We conducted t...
Article
Background Dopamine receptor 2 (DRD2) and ghrelin receptor (GHSR1a) agonists both stimulate defecation by actions at the lumbosacral defecation center. Dopamine is in nerve terminals surrounding autonomic neurons of the defecation center, whereas ghrelin is not present in the spinal cord. Dopamine at D2 receptors generally inhibits neurons, but at...
Article
Full-text available
With increases in the frequency, intensity and duration of heat waves forecast plus expansion of tropical agriculture, heat stress (HS) is both a current and an emerging problem. As cinnamon has been shown to increase insulin sensitivity, which is part of the adaptive response to HS, the aim of this experiment was to determine if cinnamon could imp...
Article
Full-text available
SFPQ is a ubiquitous nuclear RNA-binding protein implicated in many aspects of RNA biogenesis. Importantly, nuclear depletion and cytoplasmic accumulation of SFPQ has been linked to neuropathological conditions such as Alzheimer's disease (AD) and amyotrophic lateral sclerosis (ALS). Here, we describe a molecular mechanism by which SFPQ is mislocal...
Article
Background: Chronic stress exacerbates motor deficits and increases dopaminergic cell loss in several rodent models of Parkinson's disease (PD). However, little is known about effects of stress on gastrointestinal (GI) dysfunction, a common non-motor symptom of PD. We aimed to determine whether chronic stress exacerbates GI dysfunction in the A53T...
Article
Full-text available
Background: Because M1 muscarinic receptors are expressed by enteric neurons, we investigated whether positive allosteric modulators of these receptors (M1PAMs) would enhance colorectal propulsion and defecation in dogs, mice, and rats. Methods: The potencies of the M1PAMs, T662 or T523, were investigated using M1 receptor-expressing CHO cells....
Conference Paper
Progeny of primiparous sows (gilts) have poor production performance compared to those of multiparous sows. This has been attributed to underlying biological events that occur early in life. We specifically focused on the development of the gastrointestinal tract (GIT) and its function in sow vs gilt progeny (SP vs GP) reared under commercial condi...
Article
Full-text available
We use a monoclonal antibody against the C-terminal of oxyntomodulin (OXM) to investigate enteroendocrine cells (EEC) in mouse, rat, human and pig. This antibody has cross-reactivity with the OXM precursor, glicentin (Gli) but does not recognise glucagon. The antibody stained EEC in the jejunum and colon of each species. We compared OXM/Gli immunor...
Article
Full-text available
Heat stress (HS) is an environmental stressor challenging poultry production and requires a strategy to cope with it. A total of 288-day-old male broiler chicks were fed with one of the following diets: basal diet, basal with betaine (BET), or with selenium and vitamin E (AOX), or with a combination of BET and AOX, under thermoneutral and cyclic HS...
Article
Pain associated with skeletal pathology or disease is a significant clinical problem, but the mechanisms that generate and/or maintain it remain poorly understood. In this study, we explored roles for GDNF, neurturin and artemin signaling in bone pain using male Sprague-Dawley rats. We have shown that inflammatory bone pain involves activation and...
Article
Full-text available
In laboratory animals and in human, centrally penetrant ghrelin receptor agonists, given systemically or orally, cause defecation. Animal studies show that the effect is due to activation of ghrelin receptors in the spinal lumbosacral defecation centers. However, it is not known whether there is a physiological role of ghrelin or the ghrelin recept...
Article
Full-text available
Sequestration of nerve growth factor has been used successfully in the management of pain in animal models of bone disease and in human osteoarthritis. However, the mechanisms of nerve growth factor-induced bone pain and its role in modulating inflammatory bone pain remain to be determined. In this study, we show that nerve growth factor receptors...
Article
Full-text available
Recent studies have shown that patterns of colocalisation of hormones in enteroendocrine cells are more complex than previously appreciated and that the patterns differ substantially between species. In this study, the human sigmoid colon is investigated by immunohistochemistry for the presence of gastrointestinal hormones and their colocalisation....
Conference Paper
Progeny from primiparous sows (gilt) have lower birth weights, higher mortality and poorer performance than the progeny from multiparous sows. The reasons for this are complex but may involve poorer quality colostrum and impaired gastrointestinal tract (GIT) barrier function. With evidence that events early in life impact whole-of-life mortality an...
Article
Full-text available
The majority of 5-HT (serotonin) in the body is contained in enteroendocrine cells of the gastrointestinal mucosa. From the time of their discovery over 80 years ago, the 5-HT-containing cells have been regarded as a class of cell that is distinct from enteroendocrine cells that contain peptide hormones. However, recent studies have cast doubt on t...
Article
This work indicates that the newly developed ghrelin receptor agonist is a potent stimulant of colorectal motility and bowel emptying. • The aims were to determine the sites and mechanisms of action of HM01 in increasing defecation. • Effects of HM01 were studied in cells transfected with the ghrelin receptor, GHSR1a, and in anesthetized rats. • HM...
Article
Full-text available
Background: It has been recently demonstrated that the ghrelin receptor agonist, HM01, caused defecation in rats that were treated to provide a model for the constipation of Parkinson's disease. HM01 significantly increased fecal output and increased Fos activity in neurons of the hypothalamus and hindbrain, but not in the spinal defecation center...

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