Miryan Rivera

Miryan Rivera
Pontificia Universidad Católica del Ecuador | PUCE · Escuela de Ciencias Biológicas

About

108
Publications
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134
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Publications

Publications (108)
Article
Full-text available
The Imbabura treefrog (Boana picturata) is an underexplored source of bioactive peptides. The combination of molecular cloning and mass spectrometry allowed us to identify three new peptide families, named “Picturins” (PTR), “Pictuseptins” (PTS), and “Boanins” (BNS). PTR is composed of three 25-mer peptides, characterized by the N-terminal sequence...
Article
Full-text available
Microbial diseases have been declared one of the main threats to humanity, which is why, in recent years, great interest has been generated in the development of nanocomposites with antimicrobial capacity. The present work studied two magnetic nanocomposites based on graphene oxide (GO) and multiwall carbon nanotubes (MWCNTs). The synthesis of thes...
Preprint
Full-text available
Microbial diseases have been declared one of the main threats to humanity, which is why, in recent years, great interest has been generated in the development of nanocomposites with antimicrobial capacity. In the present work, two magnetic nanocomposites, based on Graphene Oxide (GO) and Multiwall Carbon Nanotubes (MWCNTs) were studied. The synthes...
Article
Full-text available
Antimicrobial peptides (AMPs) constitute part of a broad range of bioactive compounds present on diverse organisms, including frogs. Peptides, produced in the granular glands of amphibian skin, constitute a component of their innate immune response, providing protection against pathogenic microorganisms. In this work, two novel cruzioseptins peptid...
Article
Full-text available
The hyline tribe Lophyohylini includes 87 species of treefrogs, of which cytogenetics aspects have been studied in less than 20% of them. In order to evaluate the evolution of some of its chromosome characters (NOR position, C-bands, and DAPI/CMA3 bands), we studied the karyotypes of 21 lophyohylines, 16 of them for the first time, and analyzed the...
Article
Full-text available
Frog skin secretions contain medically-valuable molecules, which are useful for the discovery of new biopharmaceuticals. The peptide profile of the skin secretion of Agalychnis spurrelli has not been investigated; therefore, the structural and biological characterization of its compounds signify an inestimable opportunity to acquire new biologicall...
Article
In this research, we present a preliminary computational study of four Dermaseptin-related peptides from the skin exudate of the gliding tree frog Agalychnis spurrelli. Experimentally, the amino acid sequence of these peptides was elucidated through molecular cloning and tandem mass spectrometry and synthetic peptides were assayed against E. coli,...
Article
Full-text available
En esta investigación se presenta un estudio computacional de la dermaseptina SP2 (DRS-SP2) extraída de exudado de la piel de la rana Agalychnis spurrelli. Ensayos experimentales han permitido extraer, purificar y obtener la secuencia de aminoácidos de este péptido, además de demostrar sus propiedades antimicrobianas contra Escherichia coli, Staphy...
Article
Full-text available
The hylid tribe Cophomantini is a diverse clade of Neotropical treefrogs composed of the genera Aplastodiscus, Boana, Bokermannohyla, Hyloscirtus, and Myersiohyla. The phylogenetic relationships of Cophomantini have been comprehensively reviewed in the literature, providing a suitable framework for the study of chromosome evolution. Employing diffe...
Data
Information of the specimens analyzed of each species. (PDF)
Data
Cytogenetic information in Cophomantini. Differential techniques performed, chromosome number (2n) and NORs position observed in each species. (PDF)
Data
Chromosome measurements of 25 species of Boana and 3 of Hyloscirtus. (a) B. albopunctata group; (b) B. faber group; (c) B. pellucens group; (d) B. pulchella group; (e) B. punctata group; (f) B. semilineata group. (B) B chromosome in B. leucocheila. Chromosome percentage relative to the haploid set (Chromosome Mophology) Centromeric Index ± Standard...
Data
Optimization of the basic number (X) and the position of NORs (NORs) in Cophomantini on the phylogenetic hypothesis of Duellman et al. (2016). (PDF)
Data
DAPI and CMA3 staining in 20 species of Boana. The square shows heteromorphic NOR-bearing chromosome pair in B. punctata sequentially stained by the silver impregnation technique. (TIF)
Article
La resistencia bacteriana representa un problema para la salud pública, especialmente la producida por enzimas llamadas betalactamasas, que bloquean a un grupo importante de antibióticos, los betalactámicos, utilizados ampliamente para el tratamiento de infecciones en seres humanos. Las enterobacterias productoras de betalactamasas de espectro exte...
Article
Full-text available
Many amphibian species are at risk of extinction in their natural habitats due to the presence of the fungal pathogen Batrachochytrium dendrobatidis ( Bd ). For the most highly endangered species , captive assurance colonies have been established as an emergency measure to avoid extinction. Experimental research has suggested that symbiotic microor...
Data
Photometric method Control and experimental digital images from two tests: upper panels show an experiment where we visually detected antifungal activity; lower panels show an experiment where tested bacterium did not inhibit Bd growth. On the right, kernel density estimates for color composition in experimental images (solid blue line) and its mod...
Data
Accession numbers for bacteria isolated from the skin of three Atelopus species The table summarize the information of each isolate.
Article
The spermatozoa of representatives of three Neotropical frog subfamilies, Leiuperinae, Leptodactylinae and Paratelmatobiinae, were observed using Transmission Electron Microscopy, with the aim of identifying ultrastructural traits that provide insights into the phylogenetic relationships among these anurans, which are currently unclear. In the leiu...
Conference Paper
Background/Question/Methods Epidemic diseases often lead to dramatic declines in specific taxa. To understand the dynamics of epidemic diseases, surviving individuals often represent the only available source of information. We know that microbial communities present in amphibian skin play an important protective role against the pathogenic fungu...
Article
Full-text available
The combination of classical cytogenetics with molecular techniques represents a powerful approach for the comparative analysis of the genome, providing data for the systematic identification of chromosomal homologies among species and insights into patterns of chromosomal evolution within phylogenetically related groups. Here, we present cytogenet...
Article
Full-text available
For anurans, knowledge of 5S rDNA is scarce. For Engystomops species, chromosomal homeologies are difficult to recognize due to the high level of inter- and intraspecific cytogenetic variation. In an attempt to better compare the karyotypes of the Amazonian species Engystomops freibergi and Engystomops petersi, and to extend the knowledge of 5S rDN...
Article
Full-text available
The genus Engystomops is divided into two groups, namely the Duovox clade and the Edentulus clade. The species of Edentulus clade have karyotypes with 2n = 22, while E. pustulatus and E. puyango, which belong to Duovox clade, have 2n = 20. To investigate if 2n = 20 is a synapomorphy of Duovox clade, we cytogenetically analyzed all the species of th...
Article
Genetic divergence and speciation mechanisms of the Amazonian Engystomops frog have been inferred mainly from mtDNA sequences, microsatellite and male advertisement call. Although many aspects of this divergence remain unclear, cytogenetic analyses of Amazonian Engystomops populations are described and are compared to the relationships inferred fro...