Miroslava Atanassova

Møreforsking AS · https://scholar.google.com/citations?user=5ecNXeIAAAAJ&hl=en

Citation: Atanassova, M.R.; Stangeland, J.K.; Lausen, S.E.; Dahl, T.H.; Barnung, T.; Larssen, W.E. Antioxidants, ACE I Inhibitory Peptides, and Physicochemical Composition, with a Special Focus on Trace Elements and Pollutants, of SPRING Spawning Atlantic Herring (Clupea harengus) Milt and Hydrolysates for Functional Food Applications. Fishes 2024,...

Krill protein concentrates could be part of human diets as high-quality food ingredients. We have focused on food-grade enzyme hydrolysis of Antarctic krill Euphausia superba, aiming at the production of well-defined protein, phospholipid, and shell fractions. Different enzyme combinations, concentrations, and reaction times of three commercial pro...

Biomass is defined as organic matter from living organisms represented in all kingdoms. It is recognized to be an excellent source of proteins, polysaccharides and lipids and, as such, embodies a tailored feedstock for new products and processes to apply in green industries. The industrial processes focused on the valorization of terrestrial biomas...

Hydrogels based on marine natural biopolymers have extensive possibilities for application in regenerative medicine, being best fit from safety and toxicity point of view, inexpensive and commercially available materials for 3D tissue scaffold development. In this study a peptide fraction from the temperate sea cucumber species Parastichopus tremul...

Sea cucumbers are echinoderms that for centuries have been considered by some coastal nations to be an important part of traditional diets. Historical records show that the medical practices of the Chinese Ming Dynasty involved consumption of the local species for nourishing the blood, providing vital essences (jing), improving kidney disorders and...

A lot of research effort has been invested in the last 20 years to provide sound proof of the traditionally recognized bioactive properties of sea cucumbers. However, these are not yet part of the Western consumer's diet and are only appreciated as traditional food at the Asian market. After previous in vitro molecular study of the antioxidant, ant...

Objective: epicardial adipose tissue (EAT) from patients with coronary artery disease (CAD) contains higher levels of inflammatory proteins and lower adiponectin levels than subcutaneous adipose tissue (SAT), enhancing the progression of atherosclerosis. Since products from sea cucumber have anti-inflammatory properties, we investigated its effect...

A total of 143 presumptive yeast isolates were obtained from the predominant microflora of 21 short-ripened starter-free raw cow's milk cheeses made in Galicia (NW Spain), and the following 68 isolates were identified by both genotyping and sequencing methods: Yarrowia lipolytica (21 isolates), Kluyveromyces lactis (18), Debaryomyces hansenii (11),...

Of a total of 143 presumptive yeast isolates obtained from G alician (north‐west S pain) short‐ripened raw cow's milk starter‐free cheeses, 27 exhibited microbial alcohol‐conferred hemolytic ( MACH ) activity. Among the MACH ‐positive isolates, eight were identified as Y arrowia lipolytica and four as K luyveromyces lactis . These isolates of poten...

The microbiological quality of 18 commercially available in Spain ready-to-eat fish products containing Engraulidae was evaluated through application of the corresponding ISO procedures for total mesophilic aerobic microbial counts, detection and enumeration of enterobacteria, and detection of Staphylococcus spp. All isolates were identified to the...

A purified thermostable gellan lyase, produced by a thermophilic bacterium, Geobacillus stearothermophilus 98, was characterized in relation to its physicochemical properties. The gellan lyase was established to have a molecular weight of 216 kDa, defined by capillary gel electrophoresis. Amino acid analysis revealed high quantities of Lys, His, Al...

Microbiological study of seven sorts of home grown kefir grains was carried out in a search of Lactic Acid Bacteria (LAB) strains with antimicrobial activity. Grains were analyzed regarding to their qualitative and quantitative microflora composition, conditions of growth and change of pH of the culture during fermentation. Isolated LAB were tested...

Shallow population structure is generally reported for most marine fish and explained as a consequence of high dispersal, connectivity and large population size. Targeted gene analyses and more recently genome-wide studies have challenged such view, suggesting that adaptive divergence might occur even when neutral markers provide genetic homogeneit...

The EU FP7 funded project \“Bio-engineered micro Encapsulation of Active agents Delivered to Shellfish (BEADS)” is focused on mitigating the impact of marine biotoxins (ASP/DSP), microbial contamination (bacteria/norovirus) and the parasitic protozoan Bonamia ostreae on shellfish aquaculture. Purpose: to develop probiotic diets and a microencapsula...

The present research addresses the quality loss in two different ready-to-eat (RTE) seafoods. With this aim, chemical and microbiological parameters were measured in tuna lasagne (TL) and hake roe foods which were refrigerated (4°C) for up to 35 and 71 days, respectively. Both foods showed a significant lipid oxidation (peroxide and interaction com...

A multiplex real-time PCR (RT-PCR) method was developed in this work for pathogenic Vibrio parahaemolyticus detection in water and food samples. This bacterium is found in the environment and it is considered a major foodborne pathogen that poses a considerable public health risk; in fact, last year the World Health Organization (WHO) organized an...

Gene Ontology (GO) assignment (2nd level GO terms) of hake GAII contigs. A “Cellular Component”. B Molecular Function”. C “Biological Process”. (PDF)

List of 395 SNPs validated as polymorphic in this study (including 454-, GAII- SNPs and the set of loci detected using both data sets) together with the corresponding flanking sequences of approximately 120 bp (SNP alleles in brackets). (XLSX)

Gene Ontology (GO) assignment (2nd level GO terms) of hake 454 contigs. A “Cellular Component”. B Molecular Function”. C “Biological Process”. (PDF)

Results of GO enrichment analysis performed using all GAII contigs as reference set, and GAII SNP-containing contigs as test set. Significantly overrepresented GO terms are listed, together with the respective category (P: “Biological Process”; F: “Molecular Function”; C: “Cellular Component”), FDR (false discovery rate) and Fisher's Exact Test p-v...

Flowchart describing the intron-exon pipeline. (PDF)

Results of GO enrichment analysis performed using all 454 contigs as reference set, and 454 SNP-containing contigs as test set. Significantly overrepresented GO terms are listed, together with the respective category (P: “Biological Process”; F: “Molecular Function”; C: “Cellular Component”), FDR (false discovery rate) and Fisher's Exact Test p-val...

The growing accessibility to genomic resources using next-generation sequencing (NGS) technologies has revolutionized the application of molecular genetic tools to ecology and evolutionary studies in non-model organisms. Here we present the case study of the European hake (Merluccius merluccius), one of the most important demersal resources of Euro...

Six species of marine sponges collected at intertidal and sublittoral sites of the coast of Galicia (NW Spain) were screened for potential cytotoxic properties on Neuroblastoma BE(2)-M17 cell line. Exposure to Halichondria panicea, Pachymatisma johnstonia, Ophlitaspongia seriata and Haliclona sp. aqueous extracts strongly affected cell appearance,...

In recent years, there has been an increase in the production of shellfish and in global demand for seafood as nutritious and healthy food. Unfortunately, a significant number of incidences of shellfish poisoning occur worldwide, and microalgae that produce phycotoxins are responsible for most of these. Phycotoxins include several groups of small t...

In this work a sequential multiplex PCR system was designed and validated for the detection of most frequent foodborne pathogen Vibrio species in fish and seafood (Vibrio cholerae, Vibrio parahaemolyticus, Vibrio vulnificus, Vibrio alginoliticus and Vibrio mimicus). The method proposed functions in a hierarchical way, being composed of an end-point...

A novel moderately thermophilic bacterium, designated strain BT 13(T), was isolated from a geothermal water source in Dolni Bogrov, near Sofia, Bulgaria. The isolate was spore-forming, Gram-positive, facultatively anaerobic, alkalitolerant and heterotrophic, and was able to ferment a wide variety of carbon sources including d-glucose, sucrose, l-ar...

The antibacterial working range of six lysozymes was tested under ambient and high pressure, on a panel of five gram-positive (Enterococcus faecalis, Bacillus subtilis, Listeria innocua, Staphylococcus aureus and Micrococcus lysodeikticus) and five gram-negative bacteria (Yersinia enterocolitica, Shigella flexneri, Escherichia coli O157:H7, Pseudom...

A highly efficient method was developed for the isolation and purification of the periplasmic Escherichia coli lysozyme inhibitor protein Ivy. After isolation by osmotic shock from an E. coli overexpression strain, Ivy was purified to >95% purity using a single affinity chromatography step with hen egg white lysozyme as a ligand. Further, the speci...

The product of the Escherichia coli ORFan gene ykfE was recently shown to be a strong inhibitor of C-type lysozyme in vitro. The gene was correspondingly renamed ivy (inhibitor of vertebrate lysozyme), but its biological function in E. coli remains unknown. In this work, we investigated the role of Ivy in the resistance of E. coli to the bactericid...

New proteinaceous active substance produced by Lactobacillus paracasei subsp. paracasei strain M3 used as a starter for Bulgarian yellow cheese was identified and studied. It displayed bactericidal and fungistatic activities. Its activity was checked against over 60 bacterial and yeast strains. It was efficient against Bacillus subtilis ATCC 6633,...

In the present study a purified proteinaceous antimicrobial substance, produced by the Bulgarian yellow cheese strain Lactobacillus paracasei subsp. paracasei M3 has been partially sequenced by Edman degradation and Electrospray Ionisation Mass Spectrometry (ESI MS). The antimicrobial substance with molecular mass of 43 k Da has been tested in pure...

The growth curve and the bacteriocin production by Lactobacillus paracasei subsp. paracasei M3-a strain isolated from Bulgarian yellow cheese, was studied using electroconductivity measurements in the culture medium (MRS). The bacteriocin produced by this bacterium inhibits strains belonging to the Candida species as well as Bacillus subtilis ATCC...

The effects of cimetidine, a potent histamine-H2-receptor antagonist and a good -OH scavenger, on the kinetics of ferricytochrome c and NBT reduction by superoxide anions were studied. The drug dose-dependently inhibited ferricytochrome c and NBT reduction by O2- radicals, generated either in xanthine oxidase system or photochemically or directly b...

The purpose of this research was to investigate the potential interaction of conceptual representations and linguistic systems in the process of language acquisition. Language-thought interactions were studied in 80 American, 48 Finnish and 48 Polish preschool children. The research focused on the conceptual and linguistic development of space and...