Miriam Furné

University of Granada | UGR · Faculty of Science

The effect of both dietary lipid level and stocking density on digestive function was tested in young rainbow trout under culture conditions. Two dietary lipid levels (14 and 33%) and three culture densities (15, 30 and 40 kg/m³) were assayed in triplicate lots: L14/D15, L14/D30, L14/D40, L33/D15, L33/D30 and L33/D40. Intestinal activities of amyla...

All animals need the input of energy from the environment to stay alive. As a survival mechanism they have developed adaptive processes that allow them to continue living for a certain time when energy input does not occur. Periods of food deprivation are frequent in many species of fish in their natural environment. Climate changes, seasonal varia...

The oxidative status is associated with animal lifespan, metabolism, activity and circadian rhythms. The objective of this work is to study the time course of the oxidative status over a daily cycle in the plasma, liver and brain, and the changes in the plasma cortisol levels of sturgeon and trout. The knowledge of daily oxidative status will provi...

Three isoenergetic diets varying the proportion of dietary energy supplied by each of the macronutrients (carbohydrate, lipid, or protein) were delivered, to farmed sturgeon (Acipenser naccarii) and trout (Oncorhynchus mykiss), to test the possible effects on the intermediate metabolism and oxidative status in liver, white muscle, and heart. In tro...

Rainbow trout (100 g initial weight) were subjected to the combined effect of two culture densities (15 and 40 kg m⁻³, D15 and D40, respectively) and two dietary energy levels (22 and 27 MJ kg⁻¹ E22 and E27, respectively) during a 75-days experimental period. At the end of the experiment, the growth rate as well as the metabolic and oxidative statu...

Rainbow trout (100 g initial weight) were subjected to the combined effect of two culture densities (15 and 40 kg/m3; D15 and D40, respectively) and two levels of lipids in the diet (14 and 33%; L14 and L33, respectively) during a 75-day experimental period. The results showed worse values for the biometric parameters in trout cultured at higher de...

Primary head kidney leukocytes from Atlantic cod were isolated to evaluate the use of arachidonic acid and eicosapentaenoic acid by cyclooxygenases and the production of prostaglandins E(2) and E(3). The expression of cyclooxygenase genes and selected interleukin genes like Interleukin 1β, Interleukin 6, interleukin 8 and interleukin 10 were monito...

In this study, we investigated the effect of repeated handling on the oxidative state of gilthead sea bream, Sparus aurata. We determined the activity of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPX), glutathione transferase (GST), and DT‐diaphorase (DTD) in liver, diges...

The digestive enzymatic profile (amylase, lipase, proteases, trypsin, chymotrypsin and cellulases) was studied in three species of Mediterranean regular sea urchin: Arbacia lixula, Sphaerechinus granularis and Paracentrotus lívidus collected from the natural environment. A different enzymatic profile was manifested in the three studied species. A h...

Metabolic Modifications during StarvationStarvation, Physical Activity, and Muscle CapacitiesStarvation and Antioxidant Defenses: Antioxidant EnzymesStarvation and Antioxidant Defenses: Exogenous AntioxidantsPerspectivesReferences

The present work examines the temporal appearance and degree of activity of digestive enzymes of pancreatic and intestinal origin (amylase as well as neutral and alkaline proteases) together with the evolution of the ontogenic development of the intestine and pancreas during the first month of free-living (30 days post-hatch–[dph]) of the stur-geon...

The present study examines the particular metabolic strategies of the sturgeon Acipenser naccarii in facing a period of prolonged starvation (72 days) and subsequent refeeding (60 days) compared to the trout Oncorhynchus mykiss response under similar conditions. Plasma metabolites, endogenous reserves, and the activity of intermediate enzymes in li...

a b s t r a c t We studied the relative value of natural habitats, river and coastal wetlands, and artificial habitats, irrigation canal and ponds, for the conservation of an endangered fish, Iberian toothcarp, in its southernmost area of distribution, characterised by agricultural intensification. Our results show that the bulk of the population o...

Starvation is a situation undergone and tolerated by many species of fish in their natural environment in response to several factors (e.g. temperature, spawning migration, reproduction, etc.). To survive these periods of unfavorable feeding conditions, fish reduce their energy expenditures, which in a high percentage are derived from protein synth...

The aim of the present work is to conduct a comparative study of oxidative states in the nymphs of four species of Plecoptera belonging to the superfamily Perloidea: Perla marginata (Panzer, 1799) (family Perlidae), Guadalgenus franzi (Aubert, 1963), Isoperla curtate Navás, 1924, and lsoperla grammatica (Poda, 1761) (family Perlodidae) in relation...

A study has been made of the activity of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), and glutathione reductase (GR), as well as of lipid peroxidation and of glutathione, during the first stages of ontogenic development of the sturgeon Acipenser naccarii: the embryo within the egg, the free embry...

In order to assess the metabolic organization of Acipenser naccarii, a representative species of the ancient ancipenserid chondrosteans, the activity of key enzymes of the main metabolic pathways in the liver, white muscle, and heart, as well as the levels of plasma and tissue metabolites of this sturgeon species have been evaluated and compared to...

This work analyses the changes in the redox balance in two fish species: Adriatic sturgeon (Acipenser naccarii) and rainbow trout (Oncorhynchus mykiss) during starvation and refeeding period. The starvation period raised the lipid peroxidation (thiobarbituric-acid-reacting substances) levels in liver and blood, while a decline occurred in the antio...

Any attempt to save the endangered sturgeon species will depend on the availability of a sufficient stock of the fish in question, as aquaculture is a very suitable tool and has proved feasible for several sturgeon species and, additionally, it could contribute to the development of a new productive activity. The breeding of fish in captivity depe...

The digestive enzyme activities were determined in Adriatic sturgeon and rainbow trout during starvation and refeeding period. Overall, the digestive enzyme activities are affected in the same sense in both species. The protease and lipase activities were decreased later than amylase activity. Even after 1 month of starvation, both species would be...

Tesis Univ. Granada. Departamento de Biología Animal. Leída el 26 de mayo de 2008

Enzymatic antioxidant activities (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase) and lipid peroxidation were determined in different tissues (gills, heart, digestive tract, liver, white muscle, skin, red blood cells, swimbladder) of trout (Oncorhynchus mykiss) and sturgeon (Acipenser naccarii). Total tissue antioxida...

Studies on the digestive secretions in fish can elucidate certain aspects of their nutritive physiology and thereby resolve some nutritional problems, such as matching of an artificial diet to the nutritional needs of the fish. The aim of the present study was to analyse the digestive protease, amylase, and lipase activity in the Adriatic sturgeon...