Mircea Leabu

Mircea Leabu
Victor Babes National Institute of Pathology | IVB · Management

PhD

About

40
Publications
5,479
Reads
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718
Citations
Additional affiliations
October 2007 - March 2022
University of Bucharest
Position
  • PhD
Description
  • Research in applied ethics (with emphasis on bioethics, research ethics, and publication ethics)
June 2005 - present
Victor Babes National Institute of Pathology
Position
  • Head of Department
Description
  • Management of scientific activity in the Institute; Management of research projects and research activity in the lab. Fields: cell motility, cell signalling by integrins, cytoskeletal organization/reorganization, cell invasion and metastasis, anti-tumor drugs development.
September 2000 - February 2005
Robarts Research Institute
Position
  • PostDoc Position
Description
  • Research activity. Fields: mechanisms for cell morphology and motility controlling by integrins.
Education
October 2010 - June 2013
Carol Davila University of Medicine and Pharmacy
Field of study
  • Postdoctoral studies on research ethics in translational medicine and transplantation
November 1992 - November 1998
Carol Davila University of Medicine and Pharmacy
Field of study
  • Doctoral studies and research in myocardial pathology (ischemia-reperfusion injury)
October 1972 - July 1976
Gheorghe Asachi Technical University of Iasi
Field of study
  • General chemistry (inorganic, organic, physical); graduating thesis in quantum chemistry applied to the mechanism of vision (perturbational calculation of retinal-rhodopsin interaction)

Publications

Publications (40)
Article
Full-text available
Life represents a very complex phenomenon based on the organization and functions of cells, the elementary units of any living structure. The proper functioning of every cell in an organism assures its health. In addition, proper functioning also means the great ability of cells to adapt to changes in the environment and survive to the advantage of...
Article
Full-text available
UVA radiation induces multiple and complex changes in the skin, affecting epidermal cell behavior. This study reports the effects of UVA exposure on normal (HaCaT) and dysplastic (DOK) keratinocytes. The adherence, spreading and proliferation were investigated by time-lapse measurement of cell layer impedance on different matrix proteins. Prior to...
Chapter
Organization of biomolecules inside or outside the cell, at tissue level, proved to respect a micro- and/or nano-morphology and their functions are dependent on the right structuring of these micro- or nano-structures. This short paper summarizes the history of cellular nanostructures’ study from their discovery, to current efforts in deciphering t...
Article
Full-text available
There is a strong drive worldwide to discover and exploit the therapeutic potential of a large variety of plants. In this work, an alcoholic extract of Helleborus purpurascens (family Ranunculaceae) was investigated for the identification of amino acids and peptides with putative antiproliferative effects. In our work, a separation strategy was dev...
Article
Full-text available
The growing interest in scientometry stems from ethical concerns related to the proper evaluation of scientific contributions of an author working in a hard science. In the absence of a consensus, institutions may use arbitrary methods for evaluating scientists for employment and promotion. There are several indices in use that attempt to establish...
Article
Full-text available
Most, if not all, cells in the organism, at least in some period of their lifetime, secrete materials that are produced within the cell. Cell secretion is a phenomenon requiring membrane fusion at a specialized plasma membrane structure called the ‘porosome,’ which allows the material stored within secretory vesicles to be delivered to the cell’s e...
Article
Full-text available
Telocytes (TC) are cells with telopodes (Tp), very long prolongations (up to 100 μm) with an uneven caliber ( www.telocytes.com ). Factors determining the dynamics of cellular prolongations are still unknown, although previous studies showed telopode motility in TC cultures. We comparatively investigated, by time-lapse videomicroscopy, the dynamics...
Article
Full-text available
Secretion involves membrane fusion, which is important in assuring good intra- and extra-cellular organization, transport of essential nutrients, synthesis and delivery of vital cellular components, destruction and removal of damaged cellular materials and ultimately survival of every cell. Cells are compartmentalized by various membrane structures...
Article
Full-text available
More than forty years passed since Singer and Nicolson launched the fluid mosaic model related to molecular organization and dynamics of cell membranes, applicable to endomembranes as well. During this period of time, that will reach half a century soon, accumulating data all confirm, but not infirm the brilliant idea of such a model. Sometimes, th...
Article
Full-text available
Discoveries is a new peer-reviewed, open access, online multidisciplinary and integrative journal publishing high impact reviews, experimental articles, perspective articles, and editorials from all areas related to medicine, biology, and chemistry, including but not limited to: Molecular and Cellular Biology, Biochemistry, Biophysics, Genomics, Pr...
Article
The increasing complexity of life in today's world sheds new light upon academic life in general and on individual and institutional integrity in particular. For this reason, episodes of academic misconduct should be a matter of utmost concern for individuals, groups, and institutions. Plagiarism represents a complex and often controversial form of...
Article
Full-text available
UVA affects epidermal cell physiology in a complex manner, but the harmful effects have been studied mainly in terms of DNA damage, mutagenesis and carcinogenesis. We investigated UVA effects on membrane integrity and antioxidant defense of dysplastic keratinocytes after one and two hours of irradiation, both immediately after exposure, and 24 h po...
Article
Six new Cu(II), Ni(II), and VO(II) complexes (1–6) with Schiff base 1-phenyl-2,3-dimethyl-4-(1H-indole-3-carboxaldehyde)-3-pyrazolin-5-one (HL) were synthesized. The Schiff base was prepared through the condensation of 1-phenyl-2,3-dimethyl-4-amino-3-pyrazolin-5-one (antipyrine) with 1H-indole-3-carboxaldehyde. The new obtained compounds were chara...
Article
Full-text available
Many Christian scholars, if not all of them, consider Genesis to be foundational texts of the Bible and the spring for all the other doctrines of the Scripture. Therefore, I'm considering the attempt to search and find arguments for cell therapy ethical issues in the fundamental text of Genesis as a challenging and educative task. Moreover, this co...
Chapter
Tissue engineering is an interdisciplinary research area in translational medicine characterized by a considerable complexity due to the attempt to combine cells, engineered materials and appropriate biochemical factors to favor regeneration, or improvement of tissue function, ex vivo. The current advances in tissue engineering, despite several pro...
Article
The current spectacular advancement of assisted reproductive technologies (ARTs) has finally made parenthood at advanced ages possible, though not necessarily safer. Physicians and older patients all around the world are willing to undergo various painstaking in vitro fertilization protocols. Several motivations may determine both physicians and ol...
Article
Here we assess the intrinsic functions of the chemokine receptor CXCR4 in remodeling after myocardial infarction (MI) using Cxcr4 heterozygous (Cxcr4(+/-)) mice. Myocardial necrosis triggers complex remodeling and inflammatory changes. The chemokine CXCL12 has been implicated in protection and therapeutic regeneration after MI through recruiting an...
Chapter
Tissue engineering is a research field in biomedicine which has evolved rapidly during the last two decades. It is based on the isolation of cells, their proliferation in vitro on a scaffold, followed by the implantation of the construct inside a living organism. Each of the main elements from a tissue engineered construct – cells, scaffold, signal...
Article
No progress can ever be made without risk-taking. The discovery of stem cells and their improved handling brought new perspectives in tissue engineering. Nonetheless, whatever the method used to obtain stem cells (embryonic, from embryos got naturally, or by in vitro fertilization, produced by somatic cell nuclear transfer, by inducing pluripotent...
Article
Synthesis and biological activity investigation of complex compounds of Cu(II) are challenging issues because of the metal is not a xenobiotic one and the activity of ligands could be modulated by complexation. Complex combinations of Cu(II) and Pd(II) with thiosemicarbazone derivatives of 2-hydroxy-8-R-tricyclo[7.3.1.0.(2,7)]tridecane-13-one (wher...
Article
Full-text available
The assessment of caveolin-1 (Cav-1) as a marker of tumor aggressiveness in pancreatic ductal adenocarcinoma (PDAC). In this study, we examined the expression of Cav-1 in 34 human PDAC tissue samples and the associated peritumoral tissues by immunohistochemistry and western blot. Additionally, we correlated Cav-1 expression with other tissue (Ki-67...
Article
Sinonasal-inverted papilloma is a benign tumour with a high rate of recurrence, but possible malignant transformation. Therefore, investigation of predisposition to malignant transformation of sinonasal-inverted papilloma gives clinicians the opportunity for adequate treatment. Topoisomerase II-alpha (topoII-alpha) and Ki67 are markers of cell prol...
Article
We provide evidence that interstitial Cajal-like cells, previously described in human pancreas - pICC (J Cel Mol Med, 9: 169, 2005), are positive for c-kit irrespective of immunohistochemical procedures used. Various sample types (fresh cryosections or formalin-fixed, paraffin-embedded specimens), various slide pretreatments (with or without heat-i...
Article
Full-text available
Interstitial cells of Cajal (ICC) is a peculiar cell network composed of cells having processes described by the eminent Spanish neuroanatomist of the 19th century, S. Ramon y Cajal. ICC became a fascinating subject to many investigators and it is estimated that there are over 100 publications yearly on the subject related to ICC, in the last three...
Article
Membrane fusion is a sine qua non process for cell physiology. It is critical for membrane biogenesis, intracellular traffic, and cell secretion. Although investigated for over a century, only in the last 15 years, the molecular machinery and mechanism of membrane fusion has been deciphered. The membrane fusion event elicits essentially three actor...
Article
The diagnosis of uterine smooth muscle neoplasms by light microscopy is difficult. Multiple classification schemes have been proposed based on mitotic rate, nuclear atypia, and the presence or absence of necrosis. None of these classification systems has been entirely successful. This study was undertaken to evaluate the use of selected immunohisto...
Article
Summary The atomic force microscope (AFM) has provided nanoscale analyses of surfaces of cells that exhibit strong adhesive and cell spreading properties. However, it is frequently reported that prior fixation is required for reliable imaging of cells with lower adhesive properties. In the present study, the AFM is used to assess the effects of fix...
Article
Full-text available
It is known that beta1 integrins mediate the migratory response of cells to chemokine stimulation. Also, both beta1 integrins and chemokines have roles in tumor development. In the present study, the beta1 integrin-chemokine axis is assessed using human osteosarcoma (HOS) transfectant cells expressing the CXCR4 receptor for chemokine SDF-1 (CXCL12)...
Article
The ability of cells to undergo shape changes is essential for diverse cellular functions including cell growth, differentiation, and movement. The present study examines how an integration of the function of alpha2beta1 integrin with that of the receptor for epidermal growth factor (EGFR) modulates EGF-stimulated morphological changes in human rha...
Article
Electric cell-substrate impedance sensing (ECIS) was applied to assess the structure–function of α2β1 integrin, receptor for collagen and laminin. On collagen-coated gold electrodes, expression of this integrin on human rhabdomyosarcoma (RD) cells (RDX2C2) yielded a five-fold increase in resistance when compared with mock transfected RD (RDpF) cell...
Article
Keywords:timelapse videomicroscopy;3T3 fibroblast;spontaneous cell death;phase-contrast
Article
Peroxidatic activity in heart effluent was defined as a new biochemical parameter for the experimental study of myocardial ischemia. The peroxidatic reaction was determined by dot blot analysis with 3,3'-diaminobenzidine as hydrogen donor. After ischemia, the level of peroxidatic activity in heart effluent was 2-3 times higher than before. The effe...
Article
Chemical mapping of the luminal surface of normal rabbit aortic and coronary endothelium was investigated cytochemically to establish a baseline for further comparison with the biochemical changes possibly induced by the experimental hypercholesterolemia. Morphometric analysis showed that in the aortic endothelium the plasma membrane exposes a larg...
Article
We investigated the modifications that diet-induced hypercholesterolemia, in rabbit, can produce in the cell surface charge and chemistry of arterial endothelium (E) and blood monocytes (M). Weekly, up to 8 weeks, after blood samples were taken for lipid analysis and blood cell preparation, the vasculature was washed free of blood and the endotheli...

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