Milena Kulasek

Milena Kulasek
Nicolaus Copernicus University | umk · Department of Genetics

Master of Science

About

39
Publications
3,108
Reads
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83
Citations
Citations since 2016
34 Research Items
82 Citations
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Introduction
Milena Kulasek currently works at the Chair of Plant Physiology and Biotechnology , Nicolaus Copernicus University. Milena does research in Agricultural Plant Science and Plant development. Their current project is 'MicroRNAs involved in regulation of elements of auxin signal transduction pathway during generative development of yellow lupine (Lupinus luteus).'
Additional affiliations
March 2020 - present
Nicolaus Copernicus University
Position
  • Professor (Assistant)
March 2011 - February 2014
Warsaw University of Life Sciences - SGGW
Position
  • scientific laboratory technician
Education
September 2005 - June 2010
Nicolaus Copernicus University
Field of study
  • Biotechnology
September 2004 - September 2009
Nicolaus Copernicus University
Field of study
  • Philosophy

Publications

Publications (39)
Article
Full-text available
Artificial light at night (ALAN) alters circadian rhythms in animals and therefore can be a source of environmental stress affecting their physiology and behaviour. The impact of ALAN can be related to the increased light level, but also to the spectral composition of night lighting. Previous research showed that many species can be particularly se...
Article
Full-text available
Yellow lupine (Lupinus luteus L.) belongs to a legume family that benefits from symbiosis with nitrogen-fixing bacteria. Its seeds are rich in protein, which makes it a valuable food source for animals and humans. Yellow lupine is also the model plant for basic research on nodulation or abscission of organs. Nevertheless, the knowledge about the mo...
Article
It is well known that PsbS is a key protein for the proper management of excessive energy in plants. Plants without PsbS cannot trigger non‐photochemical quenching, which is crucial for optimal photosynthesis under variable conditions. Our studies showed wild‐type plants had enhanced tolerance to UV‐C‐induced cell death (CD) upon induction of light...
Article
Full-text available
The floral development in an important legume crop yellow lupine (Lupinus luteus L., Taper cv.) is often affected by the abscission of flowers leading to significant economic losses. Small non-coding RNAs (sncRNAs), which have a proven effect on almost all developmental processes in other plants, might be of key players in a complex net of molecula...
Preprint
Full-text available
Background Yellow lupine (Lupinus luteus L., Taper c.) is an important legume crop. However, its flower development and pod formation are often affected by excessive abscission. Organ detachment occurs within the abscission zone (AZ) and in L. luteus primarily affects flowers formed at the top of the inflorescence. The top flowers’ fate appears det...
Article
Full-text available
Rosnące światowe zapotrzebowanie na żywność wymaga wypracowania nowych strategii udoskonalania roślin celem zwiększenia ilości i jakości plonu. Obiecujące możliwości rozwiązania tego problemu oparte są na wykorzystaniu wciąż poszerzającej się wiedzy na temat miRNA, klasy endogennych małych cząsteczek RNA, negatywnie regulujących ekspresję genów na...
Article
Rosnące światowe zapotrzebowanie na żywność wymaga wypracowania nowych strategii udoskonalania roślin celem zwiększenia ilości i jakości plonu. Obiecujące możliwości rozwiązania tego problemu oparte są na wykorzystaniu wciąż poszerzającej się wiedzy na temat miRNA, klasy endogennych małych cząsteczek RNA, negatywnie regulujących ekspresję genów na...
Data
qPCR validation of differential expression patterns of selected unigenes. The relative quantification of the gene expression level was determined with qPCR assay using ACTIN as a reference gene. The values presented on top of each bar indicate the expression level derived from the RNA-Seq data.
Data
Annotation of L. luteus DEGs from an FAB vs. FNAB library comparison by KEGG database. (A) Distribution of DEGs into KEGG biological categories. The numbers of KEGG pathways belonging to a category are shown in brackets. (B) Classification of DEGs into the KEGG “Metabolism” category. The numbers of DEGs belonging to a pathway are shown.
Data
Annotation of L. luteus DEGs from an FPAB vs. FPNAB library comparison by KEGG database. (A) Distribution of DEGs into KEGG biological categories. The numbers of KEGG pathways belonging to a category are shown in brackets. (B) Classification of DEGs into the KEGG “Metabolism” category. The numbers of DEGs belonging to a pathway are shown.
Data
Statistical analysis of differentially expressed gene in compared libraries of L. luteus.
Data
Differential expression patterns of cell wall-related genes in library comparison of FAB vs. FNAB, FPAB vs. FPNAB, PAB vs. PNAB.
Data
Annotation of L. luteus DEGs from comparison of PAB vs. PNAB libraries by KEGG database. (A) Distribution of DEGs into KEGG biological categories. The numbers of KEGG pathways belonging to a category are shown in brackets. (B) Classification of DEGs into the KEGG “Metabolism” category. The numbers of DEGs belonging to a pathway are shown in bracket...
Data
Differential expression patterns of plant hormone signaling-related genes in library comparison of PAB vs. PNAB.
Data
List and annotation of differentially expressed genes (DEGs) between FAB and FNAB with PPDE < 0.05.
Data
The enriched pathways (KEGG) of DEGs from library comparison of FPAB vs. FPNAB.
Data
Quality filtering and statistics of raw reads obtained for eight transcriptome libraries of L. luteus.
Data
List and annotation of differentially expressed genes (DEGs) between FPAB and FPNAB with PPDE < 0.05.
Data
List and annotation of common DEGs identified in all three library comparisons of FAB vs. FNAB, FPAB vs. FPNAB, PAB vs. PNAB with FPKM (Fragment Per Kilobase of exon per Million fragments mapped) in each of the libraries.
Data
The enriched pathways (KEGG) of DEGs from library comparison of PAB vs. PNAB.
Data
List of primers used for the RT-qPCR validation and the RT-qPCR validation results.
Data
Differential expression patterns of plant hormone signaling-related genes in library comparison of FAB vs. FNAB.
Data
Differential expression patterns of plant hormone signaling-related genes in library comparison of FPAB vs. FPNAB.
Data
List and annotation of differentially expressed genes (DEGs) between PAB and PNAB with PPDE < 0.05.
Data
The enriched pathways (KEGG) of DEGs from library comparison of FAB vs. FNAB.
Article
Full-text available
Yellow lupine (Lupinus luteus L., Taper c.), a member of the legume family (Fabaceae L.), has an enormous practical importance. Its excessive flower and pod abscission represents an economic drawback, as proper flower and seed formation and development is crucial for the plant’s productivity. Generative organ detachment takes place at the basis of...
Article
Full-text available
Natural capacity has evolved in higher plants to absorb and harness excessive light energy. In naive models the majority of absorbed photon energy is radiated back as fluorescence and heat. For years the photon sensor protein PsbS was considered to play a critical role in non-photochemical quenching (NPQ) of light absorbed by PSII antennas and in i...
Article
Full-text available
Auxin affects almost all of the growth and development processes in plants. The auxin signal transduction pathway involves a number of proteins, among which the key elements are: TAAR auxin receptors (TIR1 and AFB1-3), AUX/IAA auxin response repressors and Auxin Response Factor (ARF) transcription factors. The activity of genes encoding some compon...
Conference Paper
Yellow lupine like the rest belonging to the family Fabaceae (Fabaceae L.), has important practical significance. Development of lupines flowers and fruit and are crucial for the productivity of these plants. Phytohormone auxin (IAA) plays an important role in the regulation of plant ontogeny, including apical dominance, the fruit maturation and ag...
Poster
Yellow lupine like the rest belonging to the family Fabaceae (Fabaceae L.), has important practical significance. Development of lupines flowers and fruit and are crucial for the productivity of these plants. Phytohormone auxin (IAA) plays an important role in the regulation of plant ontogeny, including apical dominance, the fruit maturation and ag...
Article
Plants constantly exposed to fluctuating environmental conditions develop signalling strategies that determine their acclimation, fitness and survival. Coordination between the different cells and tissues requires complex mechanisms of signal communication that lead to overall plant global signalling homeostasis. Numerous pathways controlling signa...
Article
Full-text available
Systemic acquired acclimation (SAA) is an important light acclimatory mechanism that depends on the global adjustments of non-photochemical quenching and chloroplast retrograde signaling. As the exact regulation of these processes is not known, we measured time-resolved fluorescence of chlorophyll a in Arabidopsis thaliana leaves exposed to excess...

Questions

Question (1)
Question
Hello,
since I work on a non-model plant - Lupinus luteus - I encounter many problems. My current problem is that I have a list of GO terms for a large number of genes, annotated to many different plant species (the transcriptome was de novo assembled), and I do not know how to filter the data to get plant-specific terms (without for example 'brain development'). Is there any way to do this? A list of plant GO terms would be very helpful, however, I can't find it.
Please, help!

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Projects

Projects (4)
Project
Auxin is a plant hormone, that is highly dependent from tissue and molecular context. In cells of stamens and pistils of developing flowers it cooperates with miR167-regulated auxin response factors ARF6 and ARF8 and coordinates cell division and elongation, which leads to synchronized development and functioning of these flower parts. On the other hand, this hormone is involved in abscission zone formation on pedicels of flowers designated to be dropped, which results in abscission of this organ. We believe these two phenomena are interrelated and inproper functioning of auxin and elements of signal transduction pathway of this hormone in pistils and stamens trigger flower abscission. This project aims to answer this question by investigation, if tissue localization of auxin as well as transcripts of ARF6, ARF8, MIR167 in stamens and pistils is different in yellow lupine (Lupinus luteus) flowers undergoing abscission and maintained on plant at various stages of their development, and wether change in the patterning of these agents leads to reversal of flower fate. In the future this study will result in the development of a method for reducing the amount of falling lupine flowers which will lead to increased yield and attractiveness of this plant to farmers.
Project
Yellow lupine (Lupinus luteus) is a European legume with high level of high-quality protein and low level of alkaloid content comparing to other crops from our climatic zone. This makes it an excellent alternative for highly allergenic and imported from overseas soybean. Nevertheless, one of the unfavorable features of this plant that discourages farmers from growing it is the phenomenon of excessive abscission of flowers and pods, and thus reduced grain yield. I am exploring the world of miRNAs, looking for cues that influence auxin signaling during generative development, which may also shed light on the process of abscission and result in the development of technology, that would reduce the yield losses.