Milena Đorđević

Milena Đorđević
Fruit Research Institute · Department of pomology and fruit breeding

PhD

About

54
Publications
7,575
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229
Citations
Citations since 2016
35 Research Items
189 Citations
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2016201720182019202020212022010203040
2016201720182019202020212022010203040
2016201720182019202020212022010203040

Publications

Publications (54)
Article
Full-text available
Background and AimsInterspecific hybridization and polyploidy are key processes in plant evolution and are responsible for ongoing genetic diversification in the genus Sorbus (Rosaceae). The Avon Gorge, Bristol, UK, is a world 'hotspot' for Sorbus diversity and home to diploid sexual species and polyploid apomictic species. This research investigat...
Article
Factors such as the stage of ovule maturity and longevity at flowering, when the stigma is able to receive the pollen, are significant in terms of fruit set. Various abnormalities during ovule development can often limit pollination, effective pollination period (EPP) and fruit set. In the plum cultivar 'Pozna Plava' (Prunus domestica L.), in the o...
Article
Full-text available
A newly released, late ripening plum cultivar ‘Pozna Plava’ sets fruit poorly, although it produces high quality fruit. This study aimed to evaluate which factors in the reproductive process could be related to the lack of fruit set. In two consecutive years, establishment of a suitable polleniser and the stage of ovule development at anthesis as w...
Article
Full-text available
Pollen grain micromorphological characterisation of four plum (Prunus domestica L.) genotypes from two different breeding programmes (Fruit Research Institute, Serbia, and Hohenheim, Germany) was performed using scanning electron microscopy (SEM). The studied characteristics were pollen size and shape, colpus length, mesocolpium width as well as ex...
Article
Full-text available
Since the European pear (Pyrus communis L.) is a self-incompatible fruit species, synchrony and compatibility between female parts of the mother plant and male gametes from the pollen donor must be fulfilled. Besides pollination and fertilization, normal embryo and zygote development is one of the prerequisites for the satisfactory yields in pears....
Article
Full-text available
A study was conducted to investigate the effect of different storage periods and temperatures on pollen viability in vitro and in vivo in plum genotypes ‘Valerija’, ‘Čačanska Lepotica’ and ‘Valjevka’. In vitro pollen viability was tested at day 0 (fresh dry pollen) and after 3, 6, 9 and 12 months of storage at four different temperatures (4, −20, −...
Article
Full-text available
Compatibility and synchrony between specialized tissues of the pistil, female gametophytes and male gametophytes, are necessary for successful pollination, fertilization, and fruit set in angiosperms. The aim of the present work was to study the development and viability of embryo sacs, as well as fertilization success, in relation to the fruit set...
Conference Paper
Despite numerous deficiencies, the Myrobalan seedling is the most common plum rootstock in the Republic of Serbia. Although a large number of vegetative rootstocks exists worldvide, they are generally not available to producers in our country. In this work, we investigae the influence of four vegetative rootstocks: ʻWavitʼ, ʻWeivaʼ, ʻDoceraʼ and ʻD...
Article
Norwegian plum production is characterized by climatic limitations, different flowering time, deficiently of wholly-adapted cultivars and appropriate pollen donors for cultivars that can be grown in this region. This study evaluated the progamic phase of fertilization and fruit set in four European plum cultivars (´Mallard´, ´Edda´, ´Jubileum´, and...
Conference Paper
Although there are more than 6000 plum varieties originated from different wild species from Europe, Asia and North America, there is a constant need to develop new varieties with better characteristics, tolerant/resistant to adverse environmental factors and the most important diseases, in the first place Sharka virus. In addition, one of the most...
Article
Full-text available
Ethylene is the simplest signalling molecule with a hormone-like function that plays a major role in many developmental processes, including ripening of climacteric apple fruit. The allelic polymorphisms of ACS1 and ACO1 genes, encoding for ACC synthase and ACC oxidase, which catalyse the last two steps in the ethylene biochemical pathway, were ana...
Conference Paper
Full-text available
Black-fruited (Aronia melanocarpa Michx.] Elliot) chokeberry belongs to family Rosaceae, subfamily Maloidae and has origins in eastern North America and East Canada. In the 1900s, chokeberry was transferred to Russian botanic gardens. From there, it spread in the European part of the country 1]. The name chokeberry comes from the astringency of...
Article
Full-text available
The study of ovule senescence in the ovaries of four sweet cherry cultivars (‘Karina᾿, ‘Kordia᾿, ‘Regina᾿ and ‘Summit᾿) in the environmental conditions of West Serbia was carried out. Monitoring of ovaries was performed using the fluorescence microscopy method, on emasculated and pollinated flowers (cross-pollination variant), non-emasculated open-...
Article
Full-text available
Self-incompatibility in fruits prevents self-fertilization by recognition and rejection of its own or genetically identical pollen. Occurrence of gametophyte incompatibility is accompanied by irregular pollen tube growth with an abundant accumulation of callose in it. In a three-year period, pollen tube incompatibility in the style of the plum cult...
Article
The self-compatibility of six promising plum genotypes (hybrids 38/62/70, IV/63/81, 32/21/87, 34/41/87 and 22/17/87 and cultivar ʻNada’) was examined during the period 2009–2011 through quantitative evaluation of parameters of in vivo pollen tube growth and fruit set. Mean daily temperatures were recorded during the flowering period. All genotypes...
Article
Full-text available
Investigation of initial and final fruit setting in the ‘Pozna Plava’ plum cultivar characterised by a low fruit set tendency was conducted in three pollination modes (cross-, self- and open pollination), over a two-year period (2010−2011). The crosspollination was performed using the pollen of cultivars ‘Presenta’, ‘Hanita’ and ‘Čačanska Najbolja’...
Article
Full-text available
The paper presents the main biological properties of 10 ex situ apple genotypes: ‘Bihorka’, ‘Budimka’, ‘Kraljica’, ‘Ovčiji Nos’, ‘Petrovača-1’, ‘Strekinja’, ‘Šimun Viparoš Struga’, ‘Šumnjaja’, ‘Tip 1’ and ‘Zejtinka’. The following properties of these genotypes were assessed over a two-year period: flowering phenophase; harvest maturity; pomological...
Article
Full-text available
Since 1979 to 2012 fifteen plum cultivars were named and released in Fruit Research Institute, Čačak. Some of these cultivars, such as Čačanska Lepotica, Čačanska Rodna and Čačanska Najbolja are grown in most important plum growing countries in Europe. Also, these cultivars are used as parent cultivars in many plum breeding programs. In addition to...
Article
Full-text available
The study of pollen performance in vivo of four self-incompatible sweet cherry cultivars—‘Karina’, ‘Kordia’, ‘Regina’ and ‘Summit’ was performed. The results were considered in the context of climatic conditions of West Serbia, bearing in mind increased frequency of seasons with higher temperatures during the flowering. Each cultivar was used as po...
Article
Full-text available
The study was conducted on the in vitro pollen germination at 26°, 28°, 32° and 35°C for 24h of male parental lines, pollen tube growth in vivo in cross pollination of female and male parental lines that make couples in four hybrids: ZP 504 su (� ZPPL 51 × � ZPPL 67); ZP 677 (� ZPPL 17 × � ZPPL 201); ZP 704 (� ZPPL 109 × � ZPPL 79), ZP 611 k (� ZPP...
Article
Full-text available
The pistils of plum (Prunus domestica L.) cv. 'Cacanska Lepotica' were self- and cross-pollinated in order to investigate occurrence of incompatible pollen tubes in the style, and their impact on fertilization success. The investigation also included open pollination variant. The highest percentage of these stopped the growth in the upper third of...
Article
Full-text available
Radicevic S., S. Maric, R. Cerovic, and M. Dordevic (2013): Assessment of self-(in )compatibility in some sweet cherry (Prunus avium L.) genotypes. Genetika, Vol 45, No. 3, 939-952. The paper presents results of a three-year study of self-(in)compatibility in four economically important sweet cherry genotypes – ‘Karina’, ‘Kordia’, ‘Regina’ and ‘Sum...
Article
Full-text available
The paper presents results of a three-year study of self-(in)compatibility in four economically important sweet cherry genotypes – ‘Karina’, ‘Kordia’, ‘Regina’ and ‘Summit’, under agro-environmental conditions of Western Serbia. Determination of S-RNase genotype, microscopic observation of the pollen tube growth rate and assaying of the fruit set l...
Article
Full-text available
In vitro pollen germination on agarose-sucrose medium, and pollen grain morphology by scanning electron microscopy, were investigated in four sweet cherry (Prunus avium L.) cultivars ('Karina', 'Kordia', 'Regina' and 'Summit'). The highest percentage of in vitro pollen germination over a three-year period was observed in 'Regina' (46.60 +/- 3.58%),...
Data
In vitro pollen germination and pollen grain morphology in some sweet cherry (Prunus avium L.) cultivars Abstract In vitro pollen germination on agarose-sucrose medium, and pollen grain morphology by scanning electron microscopy, were investigated in four sweet cherry (Prunus avium L.) cultivars ('Karina', 'Kordia', 'Regina' and 'Summit'). The high...
Data
In vitro pollen germination and pollen grain morphology in some sweet cherry (Prunus avium L.) cultivars Abstract In vitro pollen germination on agarose-sucrose medium, and pollen grain morphology by scanning electron microscopy, were investigated in four sweet cherry (Prunus avium L.) cultivars ('Karina', 'Kordia', 'Regina' and 'Summit'). The high...
Article
Full-text available
In vitro pollen germination and pollen grain morphology in some sweet cherry (Prunus avium L.) cultivars Abstract In vitro pollen germination on agarose-sucrose medium, and pollen grain morphology by scanning electron microscopy, were investigated in four sweet cherry (Prunus avium L.) cultivars ('Karina', 'Kordia', 'Regina' and 'Summit'). The high...
Article
Full-text available
The paper presents two-year results of the study of the initial and final fruit set in newly bred plum cultivar 'Pozna plava' grown under agroecological conditions of Serbia, which were subjected to different types of pollination. 'Pozna plava' is one of the latest ripening plum cultivars, with fruits of excellent quality, tolerant to Sharka. It ha...
Data
Full-text available
Initial and final fruit set in some plum (prunus domestica L.) hybrids under different pollination types. -Genetika, Vol 44, No. 3, 583-593. The paper presents results of two-year study (2009–2010) of initial and final fruit set in promising plum (Prunus domestica L.) hybrids developed at Fruit Research Institute – Čačak, under different pollinatio...
Article
Full-text available
Full attention has been paid to the protection of environment in recent period, thus integrated and organic cultivation methods are becoming more important in fruit production. Highly susceptible genotypes need intensive plant protection and their fruits can not rich standard quality in organic production. Growing resistant/tolerant apple, pear and...
Data
Full-text available
Fowering time and incompatibility groups – cultivar combination in commercial sweet cherry (Prunus avium L.) orchard -Genetika, Vol 43, No2, 397 -406. The paper presents results of eight-year study (1999–2006) of flowering phenophase in 21 introduced sweet cherry cultivars grown under the agro-environmental conditions of West Serbia. Flowering time...
Article
Full-text available
Unusual behavior of growing pollen tubes in different combinations of pollination was observed in the ovary of the plum (Prunus domestica L.) cv 'Čačanska Lepotica'. It primarily refers to several issues, i.e. the curling up of pollen tubes within the micropyle, the growth of two pollen tubes into the nucellus of an ovule, the occurrence...

Questions

Question (1)
Question
Hello,
Does anyone knows steps in Analysis program for starch quantification after PAS staining?
Thanks in advance!

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Projects

Projects (6)
Project
CherrySeRB is basically oriented towards the field of agro-biodiversity, aiming to add value to unexplored Serbian and Balkan fruit genetic resources through new protocols and approaches for their genotypic and phenotypic characterisation. Two fruit species of worldwide importance are targeted  diploid sweet cherry and tetraploid sour cherry. The main idea of CherrySeRB is connection of the production challenges arising from climate change and demands for quality and healthy fruits, and the richness of Serbian cherry germplasm that is still unused enough. Characterisation of the indigenous genotype potential for desirable reproductive properties and defining ʻgood reproductive behaviour cherry modelʼ that can face warmer temperature conditions during the flowering are in focus. CherrySeRB will also provide S-genotyping and the choice of suitable pollenisers that maximize fertilization efficacy for landraces, as well as their nutritional/phytochemical profiles that will enable obtaining of new high-added value nutraceutical products.Organized around four pillars that include six WPs, CherrySeRB combines interdisciplinary modern methodologies – molecular, for identification of S-haplotypes in definition of GSI-status (DNA diagnostic strategy); biological, for revealing suitable polleniser(s) (assessment of the pollen tube growth in vivo by fluorescence microscopy; the fertilization efficacy parameters); histological, for determining ovary function after exposing to different flowering temperatures (cyto-embryological and histochemical testing); nutritional, for characterisation of fruit quality and health-promoting compounds (texture and colorimetric analysis, HPLC, UV-Vis, etc.); genotype identification (S-genotyping; pollen grain characterisation by SEM); statistical (models in reaching new scientific conclusions). Data, protocols and novel cherry material will be made available to breeders, biotechnology scientists and cherry-sector stakeholders. Representing novelty in breeding approach that can be applicable to other fruit species, the ultimate goal of CherrySeRB is to foster the use of Serbian cherry germplasm as the material adapted to climate change in order to contribute to sustainable fruit production and ensure the availability of healthy food for the future.