Milan Certik

Milan Certik
Slovak University of Technology in Bratislava · Department of Biochemical Technology

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167
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Publications

Publications (167)
Preprint
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Background We conducted extractions using supercritical carbon dioxide (SC-CO 2 ) and conventional solvent methods to extract lipids and carotenoids from R. toruloides CBS 14 cells grown on wheat straw hydrolysate. The lipid extracts were analyzed using gas chromatography (GC), and the carotenoids were identified and quantified using ultra-high per...
Article
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Broiler chicken meat is the preferred meat among the human population. Broiler meat contains high-quality protein and a low-fat content, alongside a desirable fatty acid profile. A frequent problem in human nutrition is an insufficient PUFA intake in the diet. One possible strategy to increase the dietary intake of polyunsaturated fatty acids (PUFA...
Article
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Flavonoids are a group of polyphenolic compounds known to possess good radical scavenging activity. It was also shown that their anti/prooxidant properties are strongly structure‐dependent and can be modified by metal ion binding. The structural parameters also appear to be key factors during their DNA‐binding abilities. This work deals with spectr...
Article
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The global population’s increasing demand for sustainable and nutritious food sources has led to the exploration of alternative approaches in livestock production. Edible insects have emerged as a promising solution due to their nutritional composition, including high protein content, balanced fats, minerals, vitamins, and bioactive peptides. Biofe...
Article
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Punicic acid (PuA) is a polyunsaturated fatty acid with significant medical, biological, and nutraceutical properties. The primary source of punicic acid is the pomegranate seed oil obtained from fruits of trees that are mainly cultivated in subtropical and tropical climates. To establish sustainable production of PuA, various recombinant microorga...
Article
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One of the most interesting groups of fatty acid derivates is the group of conjugated fatty acids from which the most researched include: conjugated linoleic acid (CLA) and conjugated linolenic acid (CLNA), which are associated with countless health benefits. Sex pheromone mixtures of some insect species, including tobacco horn-worm (Manduca sexta)...
Preprint
One of the most interesting groups of fatty acid derivates is the group of conjugated fatty acids, from which the most researched are conjugated linoleic acid (CLA) and conjugated linolenic acid (CLNA), which are associated with countless health benefits. Sex pheromone mixture of some insect species, including tobacco horn-worm (Manduca sexta), are...
Article
Events associated with the progression of Parkinson´s disease (PD) are closely related to biomembrane dysfunction. The specific role of membrane composition in the conformational stability of alpha synuclein (αS) has already been well documented. Administration of rotenone is one of the best strategies to initiate PD phenotype in animal models. In...
Article
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Background Very long chain fatty acids (VLCFA) and their derivatives are industrially attractive compounds. The most important are behenic acid (C22:0) and erucic acid (C22:1Δ ¹³ ), which are used as lubricants, and moisturizers. C22:0 and C22:1Δ ¹³ have also potential for biofuel production. These fatty acids are conventionally obtained from plant...
Preprint
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Background Very long chain fatty acids and their derivatives are industrially attractive compounds. The most important are behenic and erucic fatty acids which are used as lubricants, moisturizers and has potential in biofuel production. These fatty acids are conventionally obtained from plant oils. Yarrowia lipolytica is an oleaginous yeast with l...
Article
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Western diets are dominated by the consumption of chemically modified foods, characterized by a deficiency of n-3 polyunsaturated fatty acids (PUFAs). Lack of n-3 PUFAs is also present in livestock feed, which negatively affects livestock health, including immune response, and results in a low content of n-3 PUFAs in animal products. The goal of th...
Article
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Red yeasts, mainly included in the genera Rhodotorula, Rhodosporidiobolus, and Sporobolomyces, are renowned biocatalysts for the production of a wide range of secondary metabolites of commercial interest, among which lipids, carotenoids, and other isoprenoids. The production of all these compounds is tightly interrelated as they share acetyl-CoA an...
Article
Pomegranate residues (PRs), the solid residues comprising peels and seeds after juice extraction, are generated in abundance in Greece and contain a variety of carbon sources. Therefore, these residues can be regarded as a potential feedstock for chemical and biotechnological processes. The polysaccharides contained in PRs were extracted and hydrol...
Preprint
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Pomegranate residues (PRs) (i.e. the solid residues remaining after juice extraction), generated currently in abundance in Greece, contain a variety of carbon sources and therefore can be regarded as a potential feedstock for chemical and biotechnological processes rather than as waste materials. In the current project, the polysaccharides containe...
Article
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The method of solid-state fermentation (SSF) represents a powerful technology for the fortification of animal-based by-products. Oleaginous Zygomycetes fungi are efficient microbial cell factories used in SSF to valorize a wide range of waste and rest cereal materials. The application of this fermentation technique for utilization and biotransforma...
Article
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Fatty alcohols (FA-OH) are aliphatic unbranched primary alcohols with a chain of four or more carbon atoms. Besides potential industrial applications, fatty alcohols have important biological functions as well. In nature, fatty alcohols are produced as a part of a mixture of pheromones in several insect species, such as moths, termites, bees, wasps...
Article
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The spindle tree (Euonymus europaeus L.) is a much-branched deciduous shrub or small tree. Its fruit capsules contain seeds with remarkably high content of oil interesting for industry, especially the 3-acetyl-1,2-diacyl-sn-glycerols (AcDAG) synthesized by a specific acetyl-CoA diacylglycerol acetyltransferase. The distribution and amount of indivi...
Article
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Flesh and fatty acid compositions, total volatile basic nitrogen (TVB-N), lipid oxidation and aerobic plate count, Enterobacteriaceae , psychrotrophic bacteria were determined in raw and smoked mackerel during cold storage (three groups differing in way of packaging; unpacked (UP), vacuum packaging (VP) and modified atmosphere (MAP) at 7, 14, 21, 2...
Article
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Solid-state fermentation is a useful tool for utilizing different plant-based materials as cultivation substrates in order to produce potentially high-value fermented bioproducts. The aim of the present study was to successfully prepare various types of such bioproducts, using a zygomycetous strain Umbelopsis isabellina CCF2412. Various legume and...
Article
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Following publication of the original article (Tao et al. 2020), the authors reported a missing funding source in the ‘Acknowledgements’ section.
Article
The specialization of parasitic plants from the Orobanchaceae family to the heterotrophic lifestyle caused several morphological, physiological and molecular changes. One of the adaptations to the parasitic lifestyle is the production of a large number of the smallest seeds in world flora, also called “dust-seeds”. Seeds of 34 holoparasitic species...
Article
Full-text available
Solid-state fermentation (SSF) is a powerful fermentation technology for valorizing rest materials and by-products of di�erent origin. Oleaginous Zygomycetes fungi are often used in SSF as an e�ective cell factory able to valorize a wide range of hydrophilic and hydrophobic substrates and produce lipid-enriched bioproducts. In this study, for the f...
Data
Proceedings of scientific papers range across a breadth of research in animal physiology. The main chapters of this publication are “Animal Physiology - Health Status Observations; Biologically Active Compounds in Animal Physiology; Animal Toxicology.” Animal physiology is the scientific study of the life-supporting properties, functions, and proc...
Chapter
We studied the growth of sheep rumen ciliates in the artificial rumen. Four experiments were performed on three different diets (D1-D3); with the high proportion of roughage in the basal diet (80:20; D1, fresh alfalfa+ground barley; D2, hay+ground barley,) and with the medium proportion of roughage (60:40; D3, fresh alfalfa+ground barley). In each...
Article
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Mucoromycota fungi possess a versatile metabolism and can utilize various substrates for production of industrially important products, such as lipids, chitin/chitosan, polyphosphates, pigments, alcohols and organic acids. However, as far as commercialisation is concerned, establishing industrial biotechnological processes based on Mucoromycota fun...
Article
Full-text available
The aim of this study was to investigate the effect of the addition of fungal solid-state fer-mented product (FP) enriched with gamma-linolenic acid (GLA) and β-carotene to feed on the haematological and immunological parameters of broiler chickens. Eighty 1-day-old COBB 500 broiler chickens were divided into two groups. The control group was fed w...
Article
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The impact of the broiler diet modification on the following parameters was evaluated: meat quality, carcass traits, and bone and blood parameters. One hundred twenty one-day-old COBB 500 broiler chickens were assigned to three experimental groups (40 birds per group) with four replications (10 per pen) for 35 days of fattening. The control (C) was...
Article
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Erucic acid (C22:1Δ13) has several industrial applications including its use as a lubricant, surfactant, and biodiesel and composite material constituent. It is produced by plants belonging to the Brassicaceae family, especially by the high erucic acid rapeseed. The ability to convert oleic acid into erucic acid is facilitated by FAE1. In this stud...
Article
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The mitochondrial citrate transport system, composed of citrate and malate transporters (MTs), can regulate the citrate efflux from mitochondria to cytosol, and then citrate is cleaved into OAA and acetyl-CoA which can be used for fatty acid (FA) biosynthesis. However, in the fungus Mucor circinelloides the molecular mechanism of citrate efflux fro...
Article
Solid-state fermentation is a technique employing microorganisms grown on a solid substrate in the absence of free water. The substrates used in this process are mostly waste from the agro-industry (brans, spent malt grains, distiller grains, etc.) that improves not only the economy of the process but also has positive effect on waste management pr...
Article
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Sixteen cold-adapted reddish-pigmented yeast strains were obtained from environmental samples. According to the PCR-based detection of classical yeast markers combined with phylogenetic studies, the yeasts belong mainly to the genera Rhodotorula, Sporobolomyces and Cystobasidium, all within the subphylum Pucciniomycotina. All strains produced carot...
Article
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The 3‐acetyl‐1,2‐diacylglycerols (acTAG) are the molecules that are structurally similar to triacylglycerols (TAG). They are naturally produced by plants of the family Celastraceae and animals such as Cervus nippon and Eurosta solidaginis. The presence of acetate in the sn–3 position of the glycerol backbone confers advantages to these compounds, e...
Article
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Here, we described an efficient method for the separation of hemicellulose, cellulose and lignin from the biomass. Solvent treatment in the presence of terephthalic acid was used to separate the lignin and hemicellulose fragments from the wheat straw. The obtained aqueous solution containing mostly xylooligomers, is free of the acid catalyst and th...
Article
Dodders (Cuscuta spp.)parasite a broad spectrum of crops and cause harvest devastation. Some efficient approaches aimed at restricting dodder distribution can be based on the control of seed germination. The corresponding processes are being studied, however, to a less extent compared to the development of vegetative or generative Cuscuta organs. T...
Article
Full-text available
The aim of this work was to analyse the effect of addition of 10% (w/w) fermented bioproduct into commercial broiler feed on fatty acid profile, lipid oxidative stability, and sensory properties of chicken breast meat. The fermented bioproduct was prepared by fermentation of cornmeal by filamentous fungi Umbelopsis isabellina CCF 2412 in solid-stat...
Article
Essential polyunsaturated fatty acids with more than two double bonds and length of carbon chain 18-22 must be taken in the diet to prevent diseases and imbalances caused by their deficiency. Terrestrial sources of polyunsaturated fatty acids are limited to only a few plant species whose large-scale cultivation is not possible and the production of...
Article
Full-text available
Gamma-linolenic acid (GLA) is a fatty acid from the ω-6 family. It is able to deliver a wide range of health benefits arising from its anti-inflammatory effects. An insufficient supply of GLA from agricultural and animal sources resulted in the development of a fermentation technique using lower filamentous fungi, which have the ability to accumula...
Article
Three strains of red yeast Rhodosporidium kratochvilovae, Rhodotorula glutinis and Sporidiobolus salmonicolor were studied for their responses to the presence metal stress, oxidative stress and a combination of these stress factors. For all yeast strains, the production of ß‐carotene increased in stress conditions. The combination of H2O2 and Zn²⁺...
Article
Full-text available
The aim of this work was to study the effect of the addition of prefermented cereal-based bioproduct (5%; BP) enriched with γ-linolenic acid to the commercial feed for broiler chickens on the production indicators, chemical composition, fatty acid profile and lipid oxidation of the meat. BP was prepared by solid-state fermentation using fungal stra...
Article
Full-text available
Red yeast Cystofilobasidium capitatum autofluorescence was studied by means of confocal laser scanning microscopy (CLSM) to reveal distribution of carotenoids inside the cells. Yeasts were cultivated in 2L fermentor on glucose medium at permanent light exposure and aeration. Samples were collected at different times for CLSM, gravimetric determinat...
Article
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Microbial utilization of raw agro-substrates by solid-state fermentation (SSF) leads to an effective enrichment of prefermented cereal-derived substrates (PCS) with oleaginous fungi being a source of ?-linolenic acid (GLA, 18:3n-6). Such method could open up new possibilities in animal nutrition. In this review, the nutritional effects of various P...
Article
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The oleaginous and red yeast Rhodotorula glutinis CCY 20-2-26 is known for its high-storage lipids accumulation in cells as well as for β-carotene biosynthesis. The work is focused on the study of lipogenesis and carotenoid pigments formation by the yeast grown in media with four different C/N ratios (20:1, 50:1, 70:1 and 100:1). Total fatty acids...
Article
Full-text available
The aim of this work was to compare the methods of malondialdehyde detection, as the main secondary product of the lipid peroxidation process, in meat and meat products. Malondialdehyde measurements were performed by two modified methods, the 2-thiobarbituric acid spectrophotometric method and the reverse-phase high-performance liquid chromatograph...
Article
Full-text available
Red yeast strains are ubiquitous microorganisms which accumulate substantial amounts of lipids and lipid-soluble metabolites. Red yeasts utilize many waste substrates of different origin. In this work red yeasts strains (Rhodotorula, Sporobolomyces, Cystofilobasidium) were used for screening of growth and metabolic activity. As a carbon source wast...
Article
Full-text available
The oleaginous red yeast Rhodotorula glutinis produces carotenoid pigments, especially torularhodin and β-carotene, in significant amounts. We have analyzed in detail carotenoid and lipid biosynthesis in a torularhodin-producing strain of R. glutinis cultivated at different carbon:nitrogen (C/N) ratios (20:1, 50:1, 70:1, and 100:1). When the strain...
Article
Full-text available
The aim of the work was to evaluate the effect of addition of prefermented bioproduct with a increased content of polyunsaturated fatty acids (especially gamma-linolenic acid) and beta-carotene into commercial feed on the selected qualitative parameters. The chemical composition, the color, the loss of water, the pH and the concentration of lactic...
Article
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Foxtail millet (Setaria italica) genotypes with red and yellow colour of grain bran were examined and compared for their content of nutritive components, fatty acids and phenolic compounds profile. Moreover, the antioxidant properties were investigated using three different methods. Foxtail millet genotypes contained 14.2% of protein, 5.0% of fat a...
Article
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The European Union has an obligation to ensure that feed produced in the European Union is safe for animals and also humans by ensuring food of animal origin is safe and wholesome. An increasing demand for safe, wholesome and nutritious animal products has led to the search for alternative substances in animal feed. Fermented feed has gained a lot...
Article
In the oleaginous yeast Yarrowia lipolytica, the diacylglycerol acyltransferases (DGATs) are major factors for triacylglycerol (TAG) synthesis. The Q4 strain, in which the four acyltransferases have been deleted, is unable to accumulate lipids and to form lipid bodies (LBs). However, the expression of a single acyltransferase in this strain restore...
Article
Full-text available
Oleaginous yeasts are considered as natural single cell oil producers. Engineering the lipid biosynthetic pathway has potential to increase lipid accumulation of these yeasts. In Yarrowia lipolytica three diacylglycerol acyltransferases encoded by LRO1, DGA1, and DGA2 genes are involved in lipid formation. Strain JMY3580 was constructed by overexpr...
Article
In this research, a microbial endophytic strain obtained from the rhizosphere of the conifer Taxus baccata and designated as Streptomyces sp. AC35 (FJ001754.1 Streptomyces, GenBank) was investigated. High 16S rDNA gene sequence similarity suggests that this strain is closely related to S. odorifer. The major fatty acid profile of intracellular lipi...
Article
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Cereals as one of the main component in human diet and feed industry are important sources of several macronutrients (proteins, carbohydrates) and micronutrients (minerals, vitamins). However, their nutritional and functional properties and subsequent applications could be improved significantly by solid state fermentations. During this biotechnolo...
Article
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Copper is a natural fungicide and is the active component of various pesticides. We detected uptake of higher concentration of the copper ions and responses to this stress in combination with presence hydrogen peroxide as a source of free radicals were studied on the three red yeast strains of species Rhodotorula glutinis (two strains) and Sporobol...
Article
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Cereals serve as major food supply for human population. The cereals are rich source of carbohydrates and some proteins, but they are limited in various biologically active compounds such as polyunsaturated fatty acids, carotenoid pigments, coenzyme Q10, etc. Therefore value-added cereal-derived biomaterials have been prepared by the fungal solid s...
Article
In the oleaginous yeast Yarrowia lipolytica, the diacylglycerol acyltransferases (DGATs) are major factors for triacylglycerol (TAG) synthesis. The Q4 strain, in which the four acyltransferases have been deleted, is unable to accumulate lipids and to form lipid bodies (LBs). However, the expression of a single acyltransferase in this strain restore...
Article
Full-text available
The artificial gene D6D encoding the enzyme ∆6desaturase was designed and synthesized using the sequence of the same gene from the fungus Thamnidium elegans. The original start codon was replaced by the signal sequence derived from the wheat gene for high-molecular-weight glutenin subunit and the codon usage was completely changed for optimal expre...
Article
Yarrowia lipolytica is a promising platform for single cell oil production. It is well-known for its metabolism oriented toward utilization of hydrophobic substrates and accumulation of storage lipids. Multiple copies of DGA2 under constitutive promoter were introduced into the Q4 strain, a quadruple mutant deleted for the four acyltransferases (Δd...
Article
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This work focuses on degradation of α‐tocopherol and formation of α‐tocopherol degradation products in sunflower oil heated at frying temperature. We determined the content and measured the kinetics of α‐tocopherol, α‐tocopheryl quinone, α‐tocopheryl fatty acid formation in heated sunflower oil without aeration (So) and in aerated oil (air flow 20...
Data
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The microbial (MO), borage (BO) and evening primrose (EPO) oils in the ration of 5 % (wt/wt) were supplemented to the diet consisted of meadow hay and ground barley (60:40) during six days following five day adaptation period of artificial rumen (RUSITEC) inoculated by sheep rumen fluid with A-type ciliate population. After adaptation period the fo...
Article
The role of cis-vaccenic acid (18:1n-7) in the reduction of unsaturated fatty acids toxicity was investigated in baker's yeast Saccharomyces cerevisiae. The quadruple mutant (QM, dga1Δ lro1Δ are1Δ are2Δ) deficient in enzymes responsible for triacylglycerol and steryl ester synthesis has been previously shown to be highly sensitive to exogenous unsa...
Article
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Carotenoids represent large group of various natural pigments ensuring typical coloration of plants, microorganisms and several animals. It was confirmed by many studies, that consuming these biological active compounds has positive impact for human life. Therefore, they are applied in different industrial fields, such as pharmacy, cosmetic, food,...
Article
Poppy seeds are widely used in the food industry, commonly for decoration and filling bakery products. They are rich in edible oil and therefore easily undergo oxidation reactions, which lead to the reduction in the shelf life. The goal of this study was to apply the previously developed methodology for testing the stability of 15 poppy seed cultiv...
Article
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Administration of Thamnidium elegans for effective utilization of agroindustrial materials (wheat bran) creates new perspectives for animal cereal diet enriched with fungal gamma-linolenic acid (GLA). The aim of our study was to evaluate the effect of adding prefermented cereal product containing a high amount of gamma-linolenic acid into the feed...
Article
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We investigated the influence of long-term storage (10 months) at an average ambient temperature of 25°C on oxidative stability of sunflower oils (made in Slovakia, Czech Republic, Austria, and Hungary) and their nutrients. Chemical properties were determined and changes in oxidative stability monitored. Oil samples were collected and analysed for...
Chapter
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Whey is an important surplus product of food industry. Based on the point of view, huge quantities of whey produced worldwide can represent an environmental problem with its disposal, or, due to the fact that whey is rich in fermentable nutrients, it can be considered as an attractive substrate for biotechnological production of various industriall...
Article
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Effects of various cultural conditions on biomass, lipid and Gamma-linolenic acid (GLA) production were investigated in the oleaginous fungus Cunninghamella blakesleeana-JSK2 isolated from soil. The GLA production was influenced by various factors such as growth condition (static and shaken), incubation time, pH, temperature, carbon and nitrogen so...