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Mikhail V Edelstein

Mikhail V Edelstein
Smolensk State Medical University, Smolensk, Russia · Institute of Antimicrobial Chemotherapy

PhD

About

77
Publications
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Publications

Publications (77)
Article
The K98 capsular polysaccharide (CPS) from the Acinetobacter baumannii clinical isolate, REV-1184, was studied by sugar analysis and Smith degradation along with one- and two-dimensional ¹H and ¹³C NMR spectroscopy and high-resolution electrospray ionization mass spectrometry. The CPS was found to consist of linear tetrasaccharide repeats (K-units)...
Article
Full-text available
Objective. To evaluate in vitro activity of biapenem and other clinically available carbapenems against Russian clinical isolates of Enterobacterales, Pseudomonas aeruginosa и Acinetobacter spp., including isolates with acquired fermentative mechanisms of resistance to β-lactams. Materials and Methods. A total of 3139 Enterobacterales isolates,...
Article
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Objective. To assess the in vitro activity of ceftazidime-avibactam against clinical Enterobacterales and Pseudomonas aeruginosa isolates in various regions of Russia based on results of local susceptibility testing by disk diffusion method. Materials and Methods. Overall, 160 laboratories located in 61 Russian cities participated in this surve...
Article
Currently, there is a steady growth antimicrobial resistance (AMP) worldwide. This is especially true now when antibiotic use has become uncontrolled due to its use in COVID-19 treatment regimens. Antibiotics do not have antiviral effect, their appointment is justified only with complicated forms of COVID-19. Moreover, such a massive use antibiotic...
Article
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Objective. To review the basic principles and functionality of the AMRmap online resource. Materials and Methods. The AMRmap platform was developed using the R programming language and various downloadable modules – packages. The current annually updated version of EUCAST clinical breakpoints was applied for determination of categories of susce...
Article
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Surveillance of antimicrobial resistance (AMR) is crucial for identifying trends in resistance and developing strategies for prevention and treatment of infections. Globally, AMR surveillance systems differ in terms of organizational principles, comprehensiveness, accessibility, and usability of data presentation. Until recently, the data on AMR in...
Article
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Multidrug resistance (MDR) and hypervirulence (hv) have been long considered distinct evolutionary traits for Klebsiella pneumoniae (Kp), a versatile human pathogen. The recent emergence of Kp strains combining these traits poses a serious global threat. In this article, we describe the phenotypic and genomic characteristics of an MDR hvKp isolate,...
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Acinetobacter myovirus BS46 was isolated from sewage by J. S. Soothill in 1991. We have sequenced the genome of BS46 and found it to be almost unique. BS46 contains double-stranded DNA with a genome size of 94,068 bp and 176 predicted open reading frames. The gene encoding the tailspike that presumably possesses depolymerase activity toward the cap...
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Objective. To reveal antibiotics being capable of potentiating the antimicrobial activity of colistin against multidrug- and extensively drug-resistant strains of Klebsiella pneumoniae, Acinetobacter baumannii and Pseudomonas aeruginosa. Materials and Methods. The minimum inhibitory concentrations (MIC) of colistin alone and in combination with...
Article
Objective: To study in vitro activity of antimicrobials against clinical isolates from patients with community-acquired urinary tract infections (UTIs) in different regions of Russia, Belarus and Kazakhstan in 2017-2018. Materials and methods: A total of 1260 isolates collectedin the Russian Federation, Belarus and Kazakhstanas a part of the int...
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Antimicrobial resistance is a growing threat to global public health and requires closer attention from the medical community. Antibiotic resistance monitoring systems play a key role in observing the antimicrobial susceptibility changes for rational prescribing antimicrobial therapy. Most current systems use data on a global scale; however, local...
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Objective. Objectives. To assess the rates of antibiotic resistance and production of acquired carbapenemases in nosocomial strains of Acinetobacter spp., and to determine the genotypes and prevalence of “international high-risk clones” among nosocomial strains of Acinetobacter baumannii isolated in various regions of Russia within the “MARATHON 2...
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Objective. To study in vitro activity of antimicrobials against clinical isolates from patients with communityacquired urinary tract infections (UTIs) in different regions of Russia in 2017–2018. Materials and Methods. A total of 1052 Enterobacterales isolates collected in the Russian Federation as part of a multicenter, prospective, epidemiolo...
Article
The genome of Acinetobacter baumannii clinical isolate, MAR-303, recovered in Russia was sequenced and found to contain a novel gene cluster at the A. baumannii K locus for capsule biosynthesis. The gene cluster, designated KL116, included four genes for glycosyltransferases (Gtrs) and a gene for a Wzy polymerase responsible for joining oligosaccha...
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The purpose of the study was to evaluate the frequency of isolation of multi-resistant Acinetobacter baumannii in cancer patients and identify the mechanisms of resistance to carbapenems. Material and Methods. We analyzed 942 strains of A. baumannii isolated from clinical samples of cancer patients in the period 2014–16. The level of resistance to...
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In the present study, we investigate the biological properties and genomic organization of virulent bacteriophage AM24, which specifically infects multidrug-resistant clinical Acinetobacter baumannii strains with a K9 capsular polysaccharide structure. The phage was identified as a member of the family Myoviridae by transmission electron microscopy...
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The purpose of the study was to evaluate the frequency of isolation of multi-resistant Pseudomonas aeruginosa and identify the mechanisms of resistance to carbapenems. Material and methods. We analyzed 866 strains of Pseudomonos aeruginosaisolated from clinical samples from cancer patients in the period 2014–2016. the level of resistance to piperac...
Poster
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Background: Since the first description of mobile colistin resistance genes in 2015, there have been numerous reports of its detection in enterobacteria across the globe. Here, we report for the first time the data on dissemination of plasmid mcr-1 gene in Russia from retrospective analysis of large isolate collection. Materials/methods: In the fra...
Conference Paper
Between February-2013 and November-2015 a nosocomial outbreak due to hypervirulent carbapenemase-producing Klebsiella pneumoniae (Kpn) occurred in the neurosurgical ICU in Moscow, Russia. Kpn isolate MAR14456 representing this outbreak was isolated in February-2014 from postoperative wound in a patient who later developed multiple abscesses, fatal...
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Pseudomonas aeruginosa is the most abundant bacterial species causing nosocomial infections in Russia. In this paper, we report the data on antimicrobial susceptibility of 743 isolates of P. aeruginosa collected in 63 hospitals of 22 cities of Russia in 2013-2014 as part of the national multicenter surveillance study on antimicrobial resistance of...
Article
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Acinetobacter baumannii and related species of the A. baumannii complex are the common and one of the most difficult-to-treat nosocomial pathogens. In this paper, we report the data on antimicrobial susceptibility of 568 isolates of Acinetobacter spp., including 542 A. baumannii, collected in 31 hospitals of 21 cities of Russia in 2013-2014 as part...
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Objective. To assess the prevalence and antimicrobial susceptibility of carbapenemase-producing Enterobacterales (CPE). Materials and Methods. A total of 5539 Enterobacterales isolates recovered from hospitalized patients in 52 medical institutions in 27 cities in Russia in 2014-2016 were tested. Antibiotic susceptibility testing (AST) was perf...
Article
A capsular polysaccharide (CPS) was isolated from strain MAR13-1452 of an emerging pathogen Acinetobacter baumannii and assigned type K125. The following structure of the CPS was established by sugar analysis, Smith degradation, and 1D and 2D 1H and 13C NMR spectroscopy: Proteins encoded by the KL125 gene cluster in the genome of MAR13-1452, includ...
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Objectives: To determine antibiotic susceptibility in isolates of Streptococcus pneumoniae and Haemophilus influenzae collected in 2014-16 from Russia. Methods: MICs were determined by CLSI broth microdilution and susceptibility was assessed using CLSI, EUCAST and pharmacokinetic/pharmacodynamic (PK/PD) breakpoints. Results: A total of 279 S....
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Pseudomonas aeruginosa is recognized as one of the six most important pathogens in terms of antimicrobial resistance («ESKAPE» pathogens), and included by WHO in the group of microorganisms for which the need for development of new antimicrobial agents is crucial. In 2015, P. aeruginosa was the second (after Klebsiella spp.) most common nosocomial...
Article
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A high level of resistance to carbapenems in Acinetobacter baumannii strains severely limits therapeutic possibilities. Colistin is the last resort drug against such strains, although the cases of resistance to this drug have become more frequent. This article presents the epidemiological features and genetic diversity of colistin nonsusceptible A....
Article
Objectives: To test the mutant selection window (MSW) hypothesis applied to linezolid-exposed Staphylococcus aureus and to delineate the concentration-resistance relationship, a mixed inoculum of linezolid-susceptible S. aureus cells and linezolid-resistant mutants (RMs) was exposed to linezolid multiple dosing. Methods: Three S. aureus strains...
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Full-text available
Acinetobacter baumannii is a gram‐negative, non‐fermenting aerobic bacterium which is often associated with hospital‐acquired infections and known for its ability to develop resistance to antibiotics, form biofilms, and survive for long periods in hospital environments. In this study, we present two novel viruses, vB_AbaP_AS11 and vB_AbaP_AS12, spe...
Conference Paper
Background Acinetobacter baumannii are common bacterial nosocomial pathogen caused large number of hospital infections. Different methods are using for molecular typing A. baumannii strains (PFGE, MLVA, MLST, whole-genome MLST) but only part of used regions of DNA have high discriminate rate. Present methods are characterized as difficult for routi...
Conference Paper
Full-text available
Background Acinetobacter baumannii are common bacterial nosocomial pathogen caused large number of hospital infections. Different methods are using for molecular typing A. baumannii strains (PFGE, MLVA, MLST, whole-genome MLST) but only part of used regions of DNA have high discriminate rate. Present methods are characterized as difficult for routi...
Article
Full-text available
Pseudomonas aeruginosa is the most abundant bacterial species causing nosocomial infections in Russia. In this paper, we report the data on antimicrobial susceptibility of 743 isolates of P. aeruginosa collected in 63 hospitals of 23 cities of Russia in 2013-2014 as part of the national multicenter surveillance study on antimicrobial resistance of...
Conference Paper
Full-text available
Background: Single nucleotide polymorphisms (SNPs) are the most abundant form of genetic variations in closely related microbial strains or isolates and, therefore, are widely used for studying microbial phylogeny and epidemiology. Highly informative sets of SNPs can be selected from large datasets of DNA sequences, such as entire multi-locus seque...
Conference Paper
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Background: Mycoplasma genitalium (MGE) is a sexually transmitted organism commonly treated with macrolides (ML). However, lately ML resistance in MGE has been reported with increasing frequency and associated with treatment failures. The mechanism of ML resistance in MGE involves mutations at specific positions of 23S rRNA gene. Here we report on...
Poster
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Background: We have previously reported on the clonal dissemination of XDR MBL-producing ST235 Pseudomonas aeruginosa (PAE) in Kazakhstan. Here, we assessed the prevalence of resistance to antipseudomonal drugs in established collection of PAE isolates and explored the potential linkage between the XDR phenotype, the presence of major PAE virulence...
Article
There is growing evidence of applicability of the hypothesis of the mutant selection window (MSW), i.e., the range between the MIC and the mutant prevention concentration (MPC), within which the enrichment of resistant mutants is most probable. However, it is not clear if MPC-based pharmacokinetic variables are preferable to the respective MIC-base...
Conference Paper
Full-text available
Background and objectives: Multilocus sequence typing (MLST) represents the gold standard genotyping method for many bacterial species, including Acinetobacter baumannii (ABA), being particularly useful in phylogenetic and global epidemiological studies. Two MLST schemes exist for ABA that correspond, respectively, to the University of Oxford MLST...
Article
Full-text available
Bacterial resistance studies using in vitro dynamic models are highly dependent on the starting inoculum that might or might not contain spontaneously resistant mutants (RMs). To delineate concentration-resistance relationships with linezolid-exposed Staphylococcus aureus, a mixed inoculum containing both susceptible cells and RMs was used. An RM s...
Article
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In this paper, we present evidence of long-term circulation of cefotaxime-resistant clonally related Salmonella enterica serovar Typhimurium strains over a broad geographic area. The genetic relatedness of 88 isolates collected from multiple outbreaks and sporadic cases of nosocomial salmonellosis in various parts of Russia, Belarus, and Kazakhstan...
Conference Paper
Background: Ceftaroline is a new broad-spectrum cephalosporin with enhanced activity against Gram-positive pathogens, including MRSA. The objective of the study was to determine in vitro activity of ceftaroline and other clinically available antimicrobials against major bacterial pathogens from different regions of Russian Federation. Methods: A...
Article
Full-text available
In light of the concept of the mutant selection window, i.e., the range between the MIC and the mutant prevention concentration (MPC), MPC-related pharmacokinetic indices should be more predictive of bacterial resistance than the respective MIC-related indices. However, experimental evidence of this hypothesis remains limited and contradictory. To...
Article
Full-text available
Multidrug-resistant and extensively-drug-resistant Pseudomonas aeruginosa are increasing therapeutic challenges worldwide. We did a longitudinal epidemiological and clinical study of extensively-drug-resistant P aeruginosa in Belarus, Kazakhstan, and Russia. The study was done in three prospectively defined phases: Jan 1, 2002-Dec 31, 2004; Jan 1,...
Article
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Objectives: To characterize the naturally occurring β-lactamase gene identified from a clinical isolate belonging to a novel enterobacterial species that is closely related to Rahnella spp. and Ewingella spp. Methods: Shotgun cloning and expression in Escherichia coli were performed in order to characterize this resistance determinant. Enzymatic...
Article
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Staphylococcus aureus is one of the most interesting microbial species in clinical studies. It is characterized by a wide extent of strain diversity, first of all, due to variability in virulence and pathogenicity. The aim of this study was to test the method of rapid Staphylococcus strain differentiation by a certain sign based on registration of...
Article
Clin Microbiol Infect 2012; 18: E214–E217 An Escherichia coli isolate co-producing VIM-4 metallo-β-lactamase and CTX-M-15 extended spectrum β-lactamase was recovered from the urine of a patient with head trauma in Moscow, Russia. The blaVIM-4 and blaCTX-M-15 genes were carried, respectively, by transmissible plasmids of IncW and IncI1 groups. The n...
Article
Production of extended-spectrum β-lactamases (ESBLs) is the one of most widespread and clinically significant mechanism of Enterobacteriaceae resistance towards modern β-lactam antibiotics. There are known 400 ESBLs, with the majority represented by the enzymes of TEM, SHV and CTX-M families. Oligonucleotide microarrays with colorimetric detection...
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Clin Microbiol Infect 2010; 16: 112–122 Acquired carbapenemases are emerging resistance determinants in Gram-negative pathogens, including Enterobacteriaceae, Pseudomonas aeruginosa and other Gram-negative non-fermenters. A consistent number of acquired carbapenemases have been identified during the past few years, belonging to either molecular cla...
Article
A minisequencing method based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) was developed for rapid identification of single nucleotide polymorphisms at bla(TEM) gene codons 104, 164 and 238 associated with extended-spectrum activity on TEM-type beta-lactamases. The method was validated by testing th...
Article
Full-text available
We report on a novel CTX-M extended-spectrum β-lactamase (ESBL), designated CTX-M-42, with enhanced activity toward ceftazidime. CTX-M-42 was identified in a hypermutable Escherichia coli nosocomial isolate (isolate Irk2320) and is a Pro167Thr amino acid substitution variant of CTX-M-3. By molecular typing of ESBL-producing E. coli strains previous...
Article
Full-text available
In this paper, we present evidence of long-term circulation of cefotaxime-resistant clonally related Salmonella enterica serovar Typhimurium strains over a broad geographic area. The genetic relatedness of 88 isolates collected from multiple outbreaks and sporadic cases of nosocomial salmonellosis in various parts of Russia, Belarus, and Kazakhstan...
Article
The rapid spread of acquired metallo-beta-lactamases (MBLs) among major Gram-negative pathogens is a matter of particular concern worldwide and primarily in Europe, one of first continents where the emergence of acquired MBLs has been reported and possibly the geographical area where the increasing diversity of these enzymes and the number of bacte...
Article
Full-text available
Thirty-four cefotaxime-resistant Salmonella enterica serovar Typhimurium isolates representative of the isolates that caused outbreaks of gastroenteritis in 10 hospitals in seven regions of Russia and Belarus from 1994 to 2003 were analyzed. All isolates produced the CTX-M-5-like extended-spectrum β-lactamase, which confers high-level resistance to...
Article
Full-text available
A total of 904 consecutive nosocomial isolates of Escherichia coli and Klebsiella pneumoniae collected from 28 Russian hospitals were screened for production of extended-spectrum beta-lactamases (ESBLs). The ESBL phenotype was detected in 78 (15.8%) E. coli and 248 (60.8%) K. pneumoniae isolates. One hundred fifteen isolates carried the genes for C...