About
7
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Introduction
I currently work at the lab of Prof. Shirley Knauer for molecular biology, University of Duisburg-Essen.
My research focuses on the characterization and modulation of protein-protein interactions (PPIs) to better understand their role in cellular processes and their therapeutic potential. One aspect of my work involves the use of nanobodies (VHHs), small, stable heavy-chain antibodies derived from alpacas, which are highly specific and serve as versatile tools.
Current institution
Additional affiliations
October 2022 - June 2024
Education
August 2019 - December 2022
Publications
Publications (7)
The human peptidyl‐prolyl‐ cis / trans isomerases (PPIases), Parvulin 14 and Parvulin 17, accelerate the cis / trans isomerization of Xaa‐Pro moieties within protein sequences. By modulating the respective binding interfaces of their target proteins, they play a crucial role in determining the fate of their substrates within the cell. Although both...
Recently, we attached a nanobody targeting the cancer-relevant protein Survivin to ultrasmall gold nanoparticles. This enabled intracellular uptake, Survivin crosslinking, and interference with cancer cell mitosis. Coupling nanobodies to nanosized scaffolds is universally applicable to improve the function and therapeutic potential of nanobodies in...
The AAA+ ATPase p97/VCP together with different sets of substrate-delivery adapters and accessory cofactor proteins unfolds ubiquitinated substrates to
facilitate degradation by the proteasome. The UBXD1 cofactor is connected to
p97-associated multisystem proteinopathy but its biochemical function and
structural organization on p97 has remained lar...
Kinetochores are chromatin-bound multi-protein complexes that allow high-fidelity chromosome segregation during mitosis and meiosis. Kinetochore assembly is exclusively initiated at chromatin containing Cse4/CENP-A nucleosomes. The molecular mechanisms ensuring that subcomplexes assemble efficiently into kinetochores only at centromeres, but not an...
The peptidyl-prolyl cis/trans isomerases (PPIases) Par14 and Par17 result from alternative transcription initiation of the PIN4 gene. Whereas Par14 is present in all metazoan, Par17 is only expressed in Hominidae. Par14 resides mainly within the cellular nucleus, while Par17 is translocated into mitochondria. Using photo-affinity labelling, cross-l...
AAA+ ATPase p97/valosin-containing protein (VCP)/Cdc48 is a key player in various cellular stress responses in which it unfolds ubiquitinated proteins to facilitate their degradation by the proteasome. P97 works in different cellular processes using alternative sets of cofactors and is implicated in multiple degenerative diseases. Ubiquitin regulat...
