Mikayel Ginovyan

Yerevan State University | YSU · Department of Biochemistry, Microbiology and Biotechnology

Background Antibiotic resistance has become one of the major problems facing humanity. The need for new antimicrobials has been increased dramatically. Plants are considered as one of the most promising sources for new antimicrobials discovery. Despite relatively small area, Armenia has large diversity of flora with many endemic species. In Armenia...

The aim of the research was to explore qualitative and quantitative phytochemical constituents of different extracts from Agrimonia eupatoria L., Hypericum alpestre subsp. polygonifolium (Rupr.) Avet. & Takht., Rumex obtusifolius Willd and Sanguisorba officinalis L. and analyze their bioactive constituents using TLC-bioautography technique. In TLC...

In the presented work, we aimed to investigate the antioxidant and possible neuroprotective capacity of extract of the aerial parts of Hypericum alpestre, found in high altitude Armenian landscape. The neuroprotective activity was evaluated using BV-2 wild type (WT) cells and acyl-CoA oxidase 1 (ACOX1) deficient (Acox1-/-) microglial cell lines. In...

Cancer continues to be a leading cause of death worldwide, making the development of new treatment methods crucial in the fight against it. With cancer incidence rates increasing worldwide, ongoing research must focus on identifying new and effective ways to prevent and treat the disease. The combination of herbal extracts with chemotherapeutic age...

Background: Herbal medicinal products containing Vaccinium myrtillus L. (bilberry) fruits and fruit extracts are widely available in the market. Although bilberry leaves and stems are considered as bio-waste, they contain much higher levels of phenolic compounds than fruits. The study aimed to investigate the antimicrobial and anticancer potential...

In recent years, nanotechnology has revolutionized various sectors, particularly in nanomedicine, where nanomaterials are used for diagnosis, monitoring, control, prevention, and treatment. Among these, silver nanoparticles (AgNPs) stand out due to their remarkable antimicrobial and cytotoxic properties. Traditional chemical synthesis of AgNPs pose...

The updated technology in Armenia enabled the small-scaleproduction of high-quality anti-D IgG, which is now supplied to mater-nity hospitals through state funding and ensuring whole demand of thisessential medicine effectively used to prevent hemolytic disease of thefetus and newborn (HDFN). The pharmaceutical facility, productionprocesses and qua...

The continuous increase in cancer rates, failure of conventional chemotherapies to control the disease, and excessive toxicity of chemotherapies clearly demand alternative approaches. Natural products contain many constituents that can act on various bodily targets to induce pharmacodynamic responses. This study aimed to explore the combined antica...

The presented study aimed to assess the e cacy of secondary metabolites extracted from blackcurrant, g, and grape leaves in reversing antibiotic resistance and enhancing chemotherapeutic e cacy. The viability tests were employed to assess the resistance-modifying properties of the metabolites both in bacterial cells and cancer cell-lines. To elucid...

Plants within the Hypericaceae family have been traditionally used for their medicinal properties, showcasing a wide range of effects such as antibacterial, antiviral, and antioxidant qualities. Hypericum alpestre (HA) extracts have exhibited significant cytotoxicity against various cancer cell lines. The phenolic compounds found in HA extracts hav...

In this study, the objective was to explore novel strategies for improving the efficacy of anticancer therapy. The focus was on investigating the antiproliferative effects of combining Rumex obtusifolius extract (RO) with the chemotherapeutic agent 5-Fluorouracil (5-FU) in non-small A549 lung cancer cells (NSCLC). Key factors such as the PI3K/Akt c...

Cancer poses a significant challenge in the medical field, requiring thorough investigation into its mechanisms and the development of effective treatments. Recently, there has been increasing interest in integrating drugs with metal nanoparticles, which are notable for their unique size and physicochemical properties, aiming to enhance anticancer...

Conventional treatment methods are not effective enough to fight the rapid increase in cancer cases. The interest is increasing in the investigation of herbal sources for the development of new anticancer therapeutics. This study aims to investigate the antitumor capacity of Hypericum alpestre (H. alpestre) extract in vitro and in vivo, either alon...

Plants of the genus Alchemilla are well known for their pharmacological effects. Alchemilla smirnovii Juz., also known as lady's mantle possessed high growth-inhibiting properties against cancer cells according to earlier reports. We aimed to explore the anticancer modulatory effect of A. smirnovii extract toward 5-fluorouracil and elucidate the po...

The increase in the incidence of cancer in Armenia and around the world is a serious problem, and the chemical interventions used in cancer therapy, which partially have a therapeutic effect, are often accompanied by the destruction of normal body cells. Therefore, it is necessary to search for new alternative treatments that will be highly effecti...

Conventional treatment methods are not effective enough to fight the rapid increase in cancer cases. The interest is increasing in the investigation of herbal sources for the development of new anticancer therapeutics. Particularly, much attention is given to finding combined phytochemical/chemotherapeutic treatment models to overcome drug resistan...

Backgrounds: Plant compounds are of great value due to their analgesic, anti-inflammatory, cytotoxic, and other characteristics. This study aimed to examine the chemical composition and impact of the Origanum vulgare hydro-ethanolic extract on nociceptive behavior and anti-inflammatory properties in in vivo mice models.Methods: Gas chromatography-m...

Background: Conventional treatment methods are not effective enough to fight the rapid increase in cancer cases. The interest is increasing in the investigation of herbal sources for the development of new anticancer therapeutics. Particularly, much attention is given to finding combined phytochemical/chemotherapeutic treatment models to overcome d...

The demand for plasma-derived medicines is high in low and medium-income countries, largely due to their high cost and limited accessibility to a significant portion of the population. Having its own plasma fractionation site is important for small countries for several reasons. Firstly, it ensures a steady supply of essential plasma-derived therap...

Compounds of plant origin are considered promising alternative approaches in the development of medicines for the prevention and treatment of cancer. The large diversity of herbal species still requires careful exploration as a source for new anticancer compounds. The goal of the study was to screen different herbal extracts traditionally used in A...

The present article includes data on the possible selective cytotoxic effect of extract of Ribes nigrum L. growing at high Armenian landscape. For this purpose, different non-cancer (microglial BV-2 wild type (Wt), acyl-CoA oxidase 1 (ACOX1) deficient (Acox1-/-) and cancer (human colon adenocarcinoma HT29 and human breast cancer MCF7) cell lines we...

Silver nanoparticles (Ag NPs) represent one of the most widely employed metal-based engineered nanomaterials with a broad range of applications in different areas of science. Plant extracts (PEs) serve as green reducing and coating agents and can be exploited for the generation of Ag NPs. In this study, the phytochemical composition of ethanolic ex...

Increased expression of nitric oxide synthase (NOS) is associated with different cancers such as cervical, breast, lung, brain, and spinal cord. Inhibition of NOS activity has been suggested as potential tool to prevent breast cancer. The anti-tumor therapeutic effect of L-nitro arginine methyl ester (L-NAME), NOS inhibitor, using in vivo models is...

High expression of nitric oxide (NO)-synthase has been found in different cancers like cervical, breast, and central nervous system. NO-synthase activity inhibition has been suggested as a possible tool to prevent breast cancer. The anti-tumor therapeutic effect of L-nitro arginine methyl ester (L-NAME) in vivo remains understudied. Here we hypothe...

Armenia has a large diversity of flora, which is not properly explored for the discovery of new biologically active extracts or compounds with promising anticancer therapeutic value. In this work, we investigated the anticancer potential of two Armenian herbal extracts; H. alpestre and R. obtusifolius, against 7,12-dimethylbenz(a)anthracene-induced...

Adverse effect of oxidative stress is a huge problem in medicine. In several circumstances, exogenous antioxidants are needed to regulate the amount of reactive species in the body. Plants are considered as a promising source for new antioxidant compounds. The goal of this study was to evaluate the antioxidant potential of extracts of the following...

The diversity of plants in Armenia is due to the singularity of natural environment. However, biochemical activity of these plants has not been studied well. The goal was to investigate biological activities as well as antihyperglycemic properties of Rumex obtusifolius L. in rabbits with hyperglycemia. According to obtained data, R. obtusifolius ex...

The goal was to reveal antioxidant properties of herbs Rumex obtusifolius Willd and Hypericum alpestre subsp. polygonifolium, used in Armenian traditional medicine. R. obtusifolius methanol extract (ME) had the lowest IC50 value (25.29 µg mL-1). At the presence of 100 µg mL-1 , the highest H2O2 reducing activity was observed with H. alpestre ME and...

The goal was to reveal antioxidant properties of herbs Rumex obtusifolius Willd and Hypericum alpestre subsp. polygonifolium, used in Armenian traditional medicine. R. obtusifolius methanol extract (ME) had the lowest IC50 value (25.29 µg mL-1). At the presence of 100 µg mL-1 , the highest H2O2 reducing activity was observed with H. alpestre ME and...

Background: Plantago major L. is a popular medicinal herb that has been used for treatment of various diseases in traditional health systems. we examined the antlbacterial and anti-phage activity of ethanol extracts of p. moTor collected from the Armavir province in Armenia, Materials We prepared P. mojor extracts for study by macerating raw plant...

Aims One of the strategies to combat antibiotic resistance can be the use of plant materials in combination with antibiotics, taking into account that phytochemicals can act as antibiotic resistance‐modifying agents. This can give a second life to the traditional antibiotics. The aim was to evaluate antibiotic‐modulatory effect of crude extracts fr...

Plantago major L. is a popular medicinal herb, which has been being used for treatment of various diseases in traditional health systems. The aim of present study was to examine the antibacterial and anti-phage activity of P. major ethanol extract. P. major fresh raw material extract was possessed of moderate anti-bacterial activity against Escheri...

Hyperglycemia is a metabolic condition characterized by high blood glucose level due to the disturbances of carbohydrate, lipids and protein metabolism. These disorders lead to impaired of insulin secretion and β-cell dysfunction. It is known that continuous and long-term use of synthetic anti-diabetic drugs can have side effects including hepatic...

Antibiotic resistance became one of the major problems of human race. Extensive and unrestricted antibiotic usage in aquaculture can bring to development of antimicrobial resistance in water environments which in turn can transfer to humans via horizontal gen transfer or even directly. Therefore, there is urgent need to monitor resistance patterns...

Plant phytochemicals can act as antibiotic modulating agents. Currently, this strategy is considered as one of the prospective ways to combat antibiotic resistance. The aim of this study was to explore antibiotic modulatory effect of extracts from Agrimonia eupatoria L., Hypericum alpestre subsp. polygonifolium (Rupr.) Avet. & Takht., Rumex obtusif...

The goal of this research was to explore the thermostability of active antimicrobial constituents of some Armenian herbs. The following herbs were used: Agrimonia eupatoria, Hypericum alpestre, Rumex obtusifolius and Sanguisorba officinalis. Thermal stability was determined by exploring plant extracts antimicrobial activity against Staphylococcus a...

Antibiotic resistance is an emerging challenge which humanity faces since late 20th century. The tendency to evaluate plant materials for their antimicrobial activity is increasing. The aim of this work was to evaluate crude extracts of five Armenian herbs for their antibacterial, antifungal and anti-phage activity. Plant crude extracts were obtai...

Nowadays, in the era of widespread antibiotic resistance, plants are considered as one of most prospective sources for new antimicrobial compounds. The goal of our research was to screen the antimicrobial efficiency of 48 plant parts of 28 wild plants species, which are commonly used in Armenian folk medicine. Maceration technique was used to obtai...

Plants are considered as one of most promising sources for new antimicrobials, based on the evidence of their use in folk medicine to treat various infectious diseases since ancient times. Despite relatively small area size, Armenia has large diversity of flora with many endemic species. The main goal of this study was the screening of various part...

Human and animal skin diseases of bacterial, fungal and viral nature and their complications are widespread and globally cause a serious trouble. Their prevalence is increasing mainly due to drug resistance. Consequently, demand has increased for new effective antimicrobial drugs, which also should be less toxic, possess a wider spectrum of action...