Mihnea Ioan Nicolescu

Mihnea Ioan Nicolescu
Carol Davila University of Medicine and Pharmacy | UMF CD · Faculty of Dentistry

Doctor of Medicine
Chair of Histology Regenerative Dentistry, Faculty of Dentistry, Carol Davila University of Medicine and Pharmacy

About

35
Publications
4,144
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1,026
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Introduction
Currently I am the Chair of Histology at the Faculty of Dental Medicine in Carol Davila University of Medicine and Pharmacy Bucharest and also part of the research team in Radiobiology Laboratory of Victor Babeș National Institute of Pathology in Bucharest, Romania. My current research interests are salivary glands regeneration and novel translational education methods.
Additional affiliations
October 2005 - present
Victor Babes National Institute of Pathology
Position
  • Researcher
February 2004 - present
Carol Davila University of Medicine and Pharmacy
Position
  • Lecturer
Description
  • Chair of Histology Division. Organiser of the first Romanian Module of Regenerative Dentistry.
Education
September 2014 - September 2015
King's College London
Field of study
  • Regenerative Dentistry
January 2005 - January 2012
Carol Davila University of Medicine and Pharmacy
Field of study
  • Cellular and Molecular Medicine
January 2004 - January 2009
Carol Davila University of Medicine and Pharmacy
Field of study
  • Oral and Maxillo-Facial Surgery

Publications

Publications (35)
Article
Full-text available
Background and Objectives: The dental pulp stem cells are highly proliferative and can differentiate into various cell types, including endothelial cells. We aimed to evaluate the ultrastructural characteristics of the human dental pulp cells of the permanent frontal teeth. Materials and Methods: Human adult bioptic dental pulp was collected from n...
Article
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We aimed to validate the safety and efficacy of the minimally invasive “open healing” flapless technique for post-extraction socket and alveolar ridge preservation, while assessing the alveolar bone changes. The study enrolled (n = 104) patients (0.55 sex ratio), with atraumatic extraction of (N = 135) hopeless teeth, followed by either immediate p...
Article
Full-text available
The salivary gland (SG) microvasculature constitutes a dynamic cellular organization instrumental to preserving tissue stability and homeostasis. The interplay between pericytes (PCs) and endothelial cells (ECs) culminates as a key ingredient that coordinates the development, maturation, and integrity of vessel building blocks. PCs, as a variety of...
Article
Full-text available
Background: Telocytes (TCs) are a peculiar morphological type of stromal cells. They project long and moniliform telopodes, visible on various bidimensional sections. Originally regarded as "interstitial Cajal-like cells", gastrointestinal TCs were CD34+. Further double-labelling studies found that colon TCs are negative for the expressions of the...
Article
Full-text available
An accurate identification of telocytes (TCs) was limited because of the heterogeneity of cell types expressing the markers attributed to TCs. Some endothelial lineage cells also could fit within the pattern of TCs. Such endothelial cells could line conjunctival lacunae previously assessed by laser confocal microscopy. We have been suggested that a...
Article
Specific ultrastructural anatomy of masticatory muscles is commonly referred to a general pattern assigned to striated muscles. Junctional feet consisting of calcium channels of the sarcoplasmic reticulum (i.e. the ryanodine receptors, RyRs)physically connected to the calcium channels of the t-tubules build triads within striated muscles. Functiona...
Article
Full-text available
This clinical study evaluated the outcome of bone regeneration using an open-healing approach. We performed 80 atraumatic extraction with socket preservation in n=69 patients (0.98 sex ratio). Post extraction sockets were filled with platelet-rich autologous plasma factors Endoret® (PRGF®) clots covered with fibrin membrane and were left exposed du...
Article
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Introduction: Correctly managed, immediate implant placement into fresh extraction socket is a favorable treatment option in order to reduce the overall treatment time and to increase the patient's comfort and satisfaction. Methodology: Atraumatic extractions (N = 42) with socket preservation were performed in n = 40 patients (0.74 sex ratio) follo...
Article
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The presence of hyaline cartilage has been previously documented in heart tissue of different vertebrates, ranging from birds to superior mammals. However, there is scarce published data regarding the appearance of focal deposits of hyaline‐like cartilage within the hearts of laboratory rats. Few mechanisms that could trigger the appearance of this...
Chapter
Stroma is viewed as the supportive framework of a predominant epithelial organ, comprising mostly of connective tissue, blood vessels and nerves. Since the discovery of telocytes one decade ago (Popescu and Faussone-Pellegrini J Cell Mol Med 2010;14(4):729–40), their presence was proven in several exocrine gland stromata, including major and minor...
Article
Full-text available
This review aims to trace the contour lines of regenerative dentistry, to offer an introductory overview on this emerging field to both dental students and practitioners. The crystallized depiction of the concept is a translational approach, connecting dental academics to scientific research and clinical utility. Therefore, this review begins by pr...
Article
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Diamond like carbon (DLC) is an amorphous carbon material suitable for use in a variety of in vivo biomedical applications, which may constitute a step forward in biotechnology of dental or orthopedic implants. Previous in vitro studies focusing on DLC films demonstrate that cellular behavior on DLC is cell type specific. The aim of the present stu...
Article
An innate osteogenic potential of the Schneiderian membrane (SM) is progressively assessed in studies ranging from non-human species to human subjects. It has relevance for endosteal placement and osseointegration. Nestin-expressing osteogenic progenitor cells are allegedly involved in bone formation and remodelling. Nestin phenotype was not assess...
Article
Full-text available
Mesenchymal stem cells (MSCs) are defined by in vitro conditions which include their aherence to plastic. Stro-1 is one of the best known markers of mesenchymal stem cells (MSCs). The cardiac stem cells are known to reside in atrial and ventricular niches. However, valvular stem niches of the heart are often overlooked. Similarly, the expression of...
Article
Full-text available
Experimental studies regarding coronary embryogenesis suggest that the endocardium is a source of endothelial cells for the myocardial networks. As this was not previously documented in human embryos, we aimed to study whether or not endothelial tip cells could be correlated with endocardial-dependent mechanisms of sprouting angiogenesis. Six human...
Article
Erst von Romberg was the first one to describe, in 1891, the pulmonary arterial hypertension (PAH), as a “pulmonary vascular sclerosis”. Since than, several groups and sub-groups of the condition were defined, mostly based on clinical features. The latest classification was established in 2013 in Nice, France (Simonneau et al., 2013). The complete...
Chapter
Telocytes represent a novel cellular type, discovered in the interstitial space. The peculiar ultrastructural features of telocytes include a small cellular body and very thin, long prolongations (telopodes). The moniliform aspect of telopodes is given by an alternation of thin segments (podomers, with a caliber below the resolving power of light m...
Article
Telocytes (TCs) are actually defined as being stromal cells with specific long and thin prolongations, called telopodes (Tp). They were positively identified in various tissues up to now. We report here for the first time the presence of TCs in the structure of esophagus. Such cells were identified under transmission electron microscopy (TEM) in es...
Article
The parotid histological structure includes acinar, ductal, and myoepithelial cells, surrounded by a connective stromal component. The parotid stroma is mostly regarded as an inert shell, consisting of septa, which divide the parenchyma. Telocytes were recently identified as a new stromal cell type in various organs, including exocrine pancreas. We...
Article
Pancreatic interstitial cells are located among acini, ducts, nerves, and blood vessels. They are essential for pancreas development, physiology, and for oncogenic microenvironment. We identified cells with characteristic ultrastructural features of telocytes in pancreatic interstitium. Telocytes were initially described as interstitial Cajal-like...
Article
Full-text available
A distinctive stromal cell-type, the telocyte (TC), has recently been described to send specific long prolongations (telopodes) alternating thin segments (podomers) with dilations (podoms). Even though one would expect TCs to be identified in various stromal tissues, there were not yet reported evidence of skin TCs. We aimed to check for the presen...
Article
Full-text available
Neuroregeneration is a relatively recent concept that includes neurogenesis, neuroplasticity, and neurorestoration--implantation of viable cells as a therapeutical approach. Neurogenesis and neuroplasticity are impaired in brains of patients suffering from Alzheimer's Disease or Parkinson's Disease and correlate with low endogenous protection, as a...
Article
Telocytes (TC) are interstitial cells with telopodes (Tp). These prolongations (Tp) are quite unique: very long (several tens of micrometres) and very thin (≤0.5 μm), with moniliform aspect: thin segments (podomeres) alternating with dilations (podoms). To avoid any confusion, TC were previously named interstitial Cajal-like cells (ICLC). Myocardia...
Article
Full-text available
In the last few years, a new cell type - interstitial Cajal-like cell (ICLC) - has been described in digestive and extra-digestive organs. The name has recently been changed to telocytes (TC) and their typical thin, long processes have been named telopodes (TP). To support the hypothesis that TC may also be present in human placenta and add to the...
Article
The existence of the epicardial telocytes was previously documented by immunohistochemistry (IHC) or immunofluorescence. We have also demonstrated recently that telocytes are present in mice epicardium, within the cardiac stem-cell niches, and, possibly, they are acting as nurse cells for the cardiomyocyte progenitors. The rationale of this study w...
Article
Full-text available
The assessment of caveolin-1 (Cav-1) as a marker of tumor aggressiveness in pancreatic ductal adenocarcinoma (PDAC). In this study, we examined the expression of Cav-1 in 34 human PDAC tissue samples and the associated peritumoral tissues by immunohistochemistry and western blot. Additionally, we correlated Cav-1 expression with other tissue (Ki-67...
Article
Full-text available
A lot of studies reported a strong association between beverages consumption and dental erosion. The erosive effect of soft drinks depends on their composition, pH or on individual salivary flow rate. The main methods used for analysing this effect are: profilometry, which measuring the enamel loss during demineralisation, and microscopic analysis....

Questions

Questions (29)
Question
How were these cells discovered?
Ramon y Cajal discovered a particular cell type in the gut, which he named ‘interstitial neurons’ more that 100 years ago. In the early 1970s, electron microscopy/electron microscope (EM) studies showed that indeed a special interstitial cell type corresponding to the cells discovered by Cajal is localized in the gut muscle coat, but it became obvious that they were not neurons. Consequently, they were renamed ‘interstitial cells of Cajal’ (ICC) and considered to be pace-makers for gut motility. For the past 10 years many groups were interested in whether or not ICC are present outside the gastrointestinal tract, and indeed, peculiar interstitial cells were found in: upper and lower urinary tracts, blood vessels, pancreas, male and female reproductive tracts, mammary gland, placenta, and, recently, in the heart as well as in the gut. Such cells, now mostly known as interstitial Cajal-like cells (ICLC), were given different and confusing names.
Moreover, ICLC are only apparently similar to canonical ICC. In fact, EM and cell cultures revealed very particular features of ICLC, which unequivocally distinguishes them from ICC and all other interstitial cells: the presence of 2–5 cell body prolongations that are very thin (most of them with a diameter below 0.2 microns, under resolving power of light microscopy), extremely long (tens to hundreds of microns), with a moniliform aspect (many dilations along), as well as caveolae. Given the unique dimensions of these prolongations (very long and very thin) and to avoid further confusion with other interstitial cell types (e.g. fibroblast, fibrocyte, fibroblast-like cells, mesenchymal cells), we are proposing the term TELOCYTES for them, and TELOPODES for their prolongations, by using the Greek affix ‘telos’, meaning "goal", "end", "fullfilment".
.... more about their history and the current progress on telocytes' research you may find on www.telocytes.com
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Telocytes in the heart
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Telocytes in the heart
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Telocytes in the heart

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