Mihir Lal Saha

University of Dhaka · Department of Botany

This study aimed to isolate biotechnologically important enzyme like amylase and protease producing rhizobacteria from rhizospheric soil. Soil samples were collected from the rhizospherc of five different flowering plants from Curzon hall, University of Dhaka. A good number of bacteria were isolated. Among them 12 bacterial isolates were selected f...

Bangladesh J. Bot. 51(4): 779-786, 2022 (December) DOI: https://doi.org/10.3329/bjb.v51i4.63497 ANTIBACTERIAL PROPERTIES OF MAJOR ETHNOMEDICINAL PLANTS USED BY THE LOCAL PEOPLE OF BRAHMANBARIA, BANGLADESH TAHMINA HAQUE, MOHAMMAD ZASHIM UDDIN1 , MD ABUL HASSAN AND MIHIR LAL SAHA Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh Keywo...

Magneto-biotechnological technique has also been applied in the ethanol fermentation and in the enzyme immobilization technique. Magnetic heating was used in the both cases. Heat generation was due to eddy current and hysteresis induced by alternating magnetic field was used for the enhancement of ethanol formation by immobilized yeast with Ba-ferr...

From the time of the establishment until the centennial birth anniversary of the University of Dhaka in 2021, the Department of Botany has travelled nearly a path of 82 years of its development and progress. The department currently hosts nearly 10 different disciplines on plant sciences through which young pre-graduate level students start learnin...

Plants are essential to the lives of humans, providing all our food— either directly or indirectly—as well as the oxygen we breathe. In addition, the plant kingdom is nature’s medicine cabinet because plants are the source of many of the most important remedies used today. Some plants produce beautiful flowers for which people pay hundreds of dolla...

The developed anaerobic-aerobic magnetic activated sludge (A2-MAS) reactor was found to be suitable for the textile effluent management. An azo dye (Orange II) and an anthraquinone dye (Remazol brilliant blue R) were used in the synthetic effluent (STE) for dye decolorization. The A2-MAS reactor consisted of an anaerobic tank (8.0 L) sequenced with...

The present study was undertaken to investigate the exopolysaccharide (EPS) producing bacteria from Sundarbans Mangrove Forest (SMF) soil of Bangladesh. The pH and aerobic heterotrophic bacterial counts of the soil samples ranged from 5.83 to 7.77 and 0.87 × 10 7 to 7.2 × 10 7 cfu/g, respectively. Potential 18 EPS producing bacterial isolates were...

Antibiotics resistant pathogenic bacteria are one of the emerging challenges of 21st century. Recently, researchers are looking for antibacterials (therapeutic agents) from ethnomedicinal indigenous plants to be considered new sources to meet the emerging challenges. These antibacterials could be used as an alternative to conventional antibiotics a...

Botany is the scientific study of plants. "Plants," to most people, means a wide range of living organisms from the smallest bacteria to the largest living things-the giant sequoia trees. According to Joseph Paxton, "Botany, the science of the vegetable kingdom, is one of the most attractive, most useful, and most extensive departments of human kno...

Activated sludge (AS) process has been adopted worldwide as a secondary biological treatment for wastewater. Activated sludge consists of a mixed community of microorganisms that metabolize and transform organic and inorganic substances into environmentally acceptable forms. However, activated sludge process is facing some problems with the disposa...

The present investigations were carried out to screen the PGPR isolates from five separate seasonal flower beds (Catharanthus roseus, Portulaca grandiflora, Celosia argentea, Gomphrena globosa and Impatiens balsamina) of Curzon Hall, University of Dhaka. The bacterial load of the collected soil samples ranged in between 7.60 ± 6.01 × 10 6 and 9.49...

The present work aimed to evaluate the bacteria associated with the most popular leafy salad vegetables like Coriander, Mint, and Lettuce and their antibiotic resistance pattern. Samples were collected from Rayshaheb Bazar, Nazira Bazar, and Shyam Bazar during June-August 2019. The maximum bacterial load was found in Coriander and the minimum bacte...

This study was undertaken to evaluate the removal of lipid-rich organic matter from wastewater by lipase producing bacteria. Ten potential lipase producing bacteria were isolated from lipid-rich environments in and around Dhaka Metropolitan city. Three of them produced lipase higher than 10 U/ml. These three isolates and their consortium were used...

The developed Anaerobic/Intermittent aerobic-Magnetic Activated Sludge (AI-MAS) process was found suitable for the continuous removal of phosphorus, nitrogen and organic compounds simultaneously from wastewater. This AI-MAS process reactor consisted of a small anaerobic compartment (first stage) placed at the corner of the intermittent aeration tan...

Enzyme from bacterial sources is much stable and obtained cheaply. Amylases and proteases are among the most important enzymes. During this study, indigenous amylase and protease producing bacteria were isolated from common decayed fruits and vegetable wastes viz. Papaya, Brinjal, Cucumber, Potato and Snake Gourd. Bacterial load ranged in between 0...

Pigments are one of the most significant secondary metabolites produced by microorganisms. The aim of the present study was to isolate and identify pigment-producing bacteria from the Ratargul Swamp Forest (RSF) soil, which is the one and only fresh water swamp forest of Bangladesh. Soil samples were randomly collected from 10 different quadrates (...

This study was undertaken to evaluate the removal of lipid-rich organic matter from wastewater by lipase producing bacteria. Ten potential lipase producing bacteria were isolated from lipid-rich environments in and around Dhaka Metropolitan city. Three of them produced lipase higher than 10 U/ml. These three isolates and their consortium were used...

To identify the gut bacteria of Pangasianodon hypophthalmus (striped catfish; Pangas) using classical and molecular approach was carried out. Total bacterial count (TBC) in the gut of pangas from farm and market samples were found 5.07 ± 1.70 × 106 and 1.40 ± 0.47 × 106 cfu/g, respectively. The gut microbiota of pangas was dominated by members of t...

An analysis for the abundance and diversity of amylolytic bacteria of two different soil types viz. garbage and garden soil was carried out. pH of the garbage and garden soil samples ranged between 7.73 and 9.84, 6.88 and 7.93, respectively. Average bacterial load on both NA and PYG agar media was found to be higher in garbage than garden soils. Ba...

Pangasianodon hypophthalmus is a major aquaculture commodity in Bangladesh. It is well accepted by a wide range of population and so, it has been a good source of protein in the diet of Bangladeshi people. The objective of this study was to assess fillet quality and to determine the proximate composition, amino acid, fatty acid profile of edible po...

Attempt was made to isolate and identify poly-β-hydroxybutyrate (PHB) producing bacteria from different domestic and industrial waste materials from in and around Dhaka Metropolitan city. The bacterial load of the collected samples ranged in between 5.50 ×103 and 2.52 × 107, 3.10 × 104 and 2.23 × 107, 7.50 × 103 and 1.01 × 107 cfu/g or cfu/ml in NA...

p>The endogenous bacterial contamination of plant tissue culture materials and their possible control was studied. Nine bacterial isolates were isolated from the contaminated tissue culture materials viz . potato and tea. On the basis of morphology and biochemical characters of nine isolates, seven were identified as Gram positive belonging to Baci...

Exopolysaccharides or Extracellular Polymeric Substances (EPS) are natural polymers of high molecular weight secreted by microorganisms into their environment or contained on the outside of bacterial cells working as mucus that contributes to soil aggregation as adhesives. EPSs also demonstrate properties like thickening, gelling, emulsifying etc....

Amylases are among the most important enzymes having great significance in present day applications like pharmaceutical, food, textile and paper manufacturing industries. Isolation of amylase enzyme producing bacterial isolates from fruit and vegetable wastes have manifold importance in various biotechnology industries. In this study, sampling was...

In the rhizosphere, plant-microbe associations play critical parts in major ecosystem processes. The present study was undertaken to enumerate and exploit the heterotrophic bacterial communities in tea rhizosphere as well as to study various soil physico-chemical properties in a tea garden. The pHs of the collected rhizosphere soil were acidic in n...

Availability of enzymes originating from soil microbes make them widely used for industrial processes in addition to their low cost, bulk production, environmental protection, plasticity and chemical stability. The present study deals with isolation of indigenous soil bacteria having industrially important enzyme viz. amylase, protease and lipase p...

Amylases are among the most important enzymes for commercial applications. The isolation of indigenous amylase producing bacteria from various soil and waste materials might haveof great significance in present day biotechnology. Potential indigenous amylase producing bacteria were isolated from garbage dumpsites from south Dhaka City Corporation....

Bacterial load and drug resistance pattern associated with some ready‐to‐eat (RTE) street foods such as Chatpoti, Fuchka, Singara, Panipuri, Ghugni‐muri, Chola and water of Dhaka South City Corporation were investigated. Most of the samples were found to be contaminated and the bacterial load ranged from 2.4 × 10^4 ‐ 9.2 × 10^6, 1.2 × 10^3 ‐ 7.3 ×...

Acute hepatopancreatic necrosis disease (AHPND) is an emerging shrimp disease caused by strains of Vibrio parahaemolyticus containing a unique virulent plasmid, responsible for substantial economic losses since 2009; caused up to 100% mortality in farmed shrimp Penaeus monodon. The purpose of this study was to isolate and identify the pathogenic st...

In the rhizosphere, plant-microbe associations play critical part in major ecosystem processes. The pHs of the collected rhizosphere soil were acidic (4.24-4.77) and favorable for the established tea orchards. Soil texture was either sandy clay loam or sandy loam in nature. Organic carbon of the samples ranged from 0.91-1.19%, depicting the soil fa...

The present article focuses the plant species for the treatment of diabetes used by the local people of Brahmanbaria district, Bangladesh. The data were collected using semi-structure interviews with key informants during the year of 2015 and 2016. In some cases group discussion and informal conversation with local people were also conducted. The s...

Acute Hepatopancreatic Necrosis Disease (AHPND), also called Early Mortality Syndrome (EMS), is a recently emergent shrimp bacterial disease caused by strains of Vibrio parahaemolyticus that contain a unique virulent plasmid, resulted in substantial economic losses since 2009. AHPND has caused severe mortalities up to 100% in farmed populations of...

Sundarban Mangrove Forest (SMF) is a tidal forest of Bangladesh and ecologically unique ecosystem for natural resource. SMF soils were studied for pigment producing bacteria. The pH of the collected mangrove soil samples ranged 6.02 - 7.70. The bacterial load varied from 2.68 × 10⁷ to 3.38 × 10⁷ cfu/g soil with an average bacterial count of 2.90 ×...

Introduction: Acute Hepatopancreatic Necrosis Disease (AHPND), also called Early Mortality Syndrome (EMS), is a recently emergent shrimp bacterial disease caused by strains of Vibrio parahaemolyticus, resulted in substantial economic losses causing severe mortalities up to100% in farmed Penaeus monodon. The objectives of this work was to identify t...

The river Turag is the upper tributary of the river Buriganga in Bangladesh. Aerobic heterotrophic bacterial count of the river in nutrient agar (NA) and peptone yeast extract glucose (PYG) agar medium was from 2.91×10^5 to 27.5×10^5 cfu/ml & from 1.09×10^5 to 19.3×10^5 cfu/ml, respectively. Enteric and related bacterial count on MacConkey agar was...

Aeromonas hydrophila is an opportunistic microorganism. It is a secondary biological agent that contributes to the occurrence of fish diseases and its deterioration. This research was undertaken to determine the prevalence of A. hydrophila in some freshwater fishes collected from three different fish markets of Dhaka City and to test their antibiot...

Aeromonas hydrophila is an opportunistic microorganism. It is a secondary biological agent that contributes to the occurrence of fish diseases and its deterioration. This research was undertaken to determine the prevalence of A. hydrophila in some freshwater fishes collected from three different fish markets of Dhaka City and to test their antibiot...

To isolate and characterize lipase producing bacteria from lipid-rich environment and screen the best lipolytic indigenous bacteria a study was made. For the isolation of bacteria, oil based wastewater and soil were collected from ten different sampling sites. Four different media were used for study the aerobic heterotrophic bacterial count. The h...

p>To isolate and characterize lipase producing bacteria from lipid‐rich environment and screen the best lipolytic indigenous bacteria a study was made. For the isolation of bacteria, oil based wastewater and soil were collected from ten different sampling sites. Four different media were used for study the aerobic heterotrophic bacterial count. The...

To isolate and characterize lipase producing bacteria from lipid-rich environment and screen the best lipolytic indigenous bacteria a study was made. For the isolation of bacteria, oil based wastewater and soil were collected from ten different sampling sites. Four different media were used for study the aerobic heterotrophic bacterial count. The h...

Dalbergia sissoo Roxb. is one of the most important tree species found widely distributed not only in Bangladesh but also in South Asian countries. Unfortunately, a new form of disease called die-back has been established in Sissoo trees. This research was undertaken to find out the presence and relationship of bacteria with die-back disease. Sampl...

Samples collected from different stages of the tannery industry were found to be alkaline (pH 7.52 to 12.11). A good number of bacteria were found to be associated with the samples. The bacterial count ranged in between 1.34×105 to 3.44×105 cfu/ml and 1.04×105 to 6×105 cfu/ml on nutrient agar (NA) and peptone yeast extract glucose agar (PYG) medium...

The bacteria associated with street food Chatpati and their multi-drug resistance pattern were investigated. The highest bacterial load of solid part of Chatpati was recorded on NA as 1.07x10(5) - 9.7x10(5) cfu/g followed by 1.55x10(4) - 4.05x10(5) cfu/g on EMB. In case of liquid part (Spicy tamarind) maximum load was 3.1x10(2) - 6.4x10(4) cfu/ml o...

Present study was conducted to determine the bacteria and their multi-drug resistance pattern of Velpuri and water of Velpuri shop of different areas of Dhaka city. A total of 74 bacteria were isolated of which 26 isolates were subjected for further study. Eleven and 15 isolates from 26, were found Gram-positive and Gram-negative bacteria, respecti...

Morphological and biochemical characterization of different bacteria isolated from eight fresh and salted hilsa collected from four fish markets namely Palashi Bazaar, Karwan Bazaar, Anando Bazaar and New Market of Dhaka city was carried out. Five types of bacteriological culture media namely nutrient agar as generalized complex medium, EMB agar me...

Dieback of sissoo (Dalbergia sissoo Roxb.) is a devastating plant disease, whose causative biotic agents were not yet identified unequivocally. Our previous studies revealed that bacteria belonging to the genus Pseudomonas were associated with dieback‐affected sissoo trees. To study the bacterial community associated with dieback‐affected sissoo tr...

Efficacy of aqueous suspension of Bacillus thuringiensis var. israelesis (Bti) was studied against the laboratory reared 3rd and 4th instar larvae of Aedes aegypti. Mortality rates as well as histological changes in the larval midgut due to toxic effect of Bti were investigated. Bti treated larval midgut samples were also streaked onto a Petri - di...

Bacterial and nutritional quality of fresh and salted hilsa collected from four markets of Dhaka city were investigated. Five different culture media viz. nutrient agar, EMB agar for coliform, SS agar for Salmonella-Shigella, MSA agar for Staphylococcus and TCBS for Vibrio were used. The highest heterotrophic bacterial count 1.22 ± 0.12 × 106 cfu/g...

Efficacy of aqueous suspension of Bacillus thuringiensis israelesis (Bti) was studied against the laboratory reared 3rd and 4th instar larvae of Ae. aegypti. Mortality rates as well as histological changes in the larval midgut due to toxic effect of Bti were investigated. Several microscopic techniques were also used to identify the toxic agent of...

Although soil bacterial communities are one of the important biotic components that influence decomposition and nutrient mineralization in the terrestrial ecosystems, factors driving this biotic community in the Sunderban mangrove forests are not well studied. The present study examined the importance of soil physico‐chemical properties in driving...

This work was designed to isolate iron related bacteria for possible identification and to test their potentiality in �various mineralogical processes. Iron transforming bacteria were isolated from different rusty iron materials. Thirty four isolates were selected from a total of seventy bacterial isolates. Out of 34 isolates 5 belonged to the genu...

The present study aimed at identifying the soil bacterial communities in the Madhupur Sal and the Sunderban mangrove forests, the two important forests of Bangladesh. Soil bacteria were identified through sequencing of PCR‐amplified fragments of bacterial 16S rDNA. The results showed that the bacterial communities of the Madhupur Sal forest soils a...

The aerobic heterotrophic bacterial count of four pond water samples ranged between 6.92×10 4 and 1.72×10 6 cfu/100 ml. The highest count (1.72×10 6 cfu/100 ml) was recorded in Zahurul Haq Hall pond water. Enteric bacterial count was found in between 1.69×10 4 and 3.31×10 5 cfu/100ml in water samples. A total of 79 isolates were obtained of which 2...

Key words: Enteric bacteria; lactose fermenter; non-lactose fermenter; antibiotic resistance DOI: http://dx.doi.org/10.3329/bjsr.v24i2.10769 Bangladesh J. Sci. Res. 24(2):127-134, 2011 (December)

Seasonal variation on bacterial load irrespective of heterotrophic and enteric bacteria was noticed in Gulshan lack of Dhaka Metropolitan city. The lowest number (1.56 x 10(6) cfu/100 ml) in the summer and the highest (19.03 x 10(6) cfu/100 ml) were recorded during winter. Among the isolated bacteria 32 were studied in details and provisionally ide...

Samples from Pagla sewage treatment plant at different treatment stages showed more or less similar temperature (26.9 ‐ 27.5 0 C). The pH ranged from 7.2 ‐ 7.9. Influent water and primary sedimentation tank water were brownish in colour while sludge water was light black. The lagoon water and treated water were greenish. The ammonium‐nitrogen (NH +...

Samples collected from different stages of the tannery processing were found to be alkaline. A good number of bacteria were found to be associated with the different stages of leather processing. The aerobic heterotrophic bacterial count ranged in between 11.9 × 10 6 and 46.7 × 10 6. The highest count was observed in the soaking stage and the minim...

identified unequivocally. Putative microbial pathogens (fungi and bacteria) were studied in dieback affected sissoo trees collected from Bangladesh, using plant pathological techniques combined with molecular tools. DNA based characteri-zation showed the presence of heterogeneous patterns of various fungi (mostly saprophytic). It did not support th...

This study examined the effect of deforestation on the soil properties (physico-chemical and bacterial) of the Sal (Shorea robusta Roxb. Ex Gaertn.) forests in Bangladesh. Physico-chemical properties and the bacterial colony counts of soil were studied by comparing a natural Sal forest site with deforested and planted with Menjium site, deforested...

Dieback of Dalbergia sissoo Roxb. (sissoo) is a disastrous disease, which has destroyed millions of forest trees in South Asia. Plant pathogenic fungi and bacteria were found associated with diseased trees, but the causative disease agent has not yet been identified unequivocally. In order to see whether plant viruses could be detected in diseased...

Seventy bacterial isolates were recovered from different rusted iron materials using modified NP glucose, 9K and sulfur oxidizing media. Thirty four isolates were selected after primary screening on the basis of their growth in modified NP glucose medium. Out of 34 isolates 5 belonged to the genus Kurthia, 6 Thiobacillus, 16 Bacillus and remaining...

Dieback of sissoo ( Dalbergia sissoo) has been recognized since1993 as a dramatic threat for forests and timber production on the Indian subcontinent. The causal agent(s) have not yet been identified. In a joint research project between the Department of Botany, University of Dhaka, and the Biocentre Klein Flottbek, University of Hamburg, putative...