Miguel A. Sogorb

Miguel A. Sogorb
Universidad Miguel Hernández de Elche | UMH · Institute of Bioengineering

Professor

About

145
Publications
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Publications

Publications (145)
Article
Full-text available
Quantum dots are nanoparticles with very promising biomedical applications. However, before these applications can be authorized, a complete toxicological assessment of quantum dots toxicity is needed. This work studied the effects of cadmium-selenium quantum dots on the transcriptome of T98G human glioblastoma cells. It was found that 72-h exposur...
Article
Full-text available
The world is living a pandemic situation derived from the worldwide spreading of SARS-CoV-2 virus causing COVID-19. Facemasks have proven to be one of the most effective prophylactic measures to avoid the infection that has made that wearing of facemasks has become mandatory in most of the developed countries. Silver and graphene nanoparticles have...
Chapter
The available alternative methods for testing developmental toxicity comprise either cellular models or whole embryos of rodents, fish, or amphibian. The simplest cellular models consider the use of human or animal embryonic stem cells (embryonic or of induced pluripotency) under differentiation and one of the most widely used endpoints in these me...
Chapter
The guidelines for testing the reproductive toxicity in vivo developed and validated by Organisation for Economic Cooperation and Development allow for a systematic and internationally accepted testing and assessment of chemicals. Within reproductive toxicity two main categories of guidelines are usually identified: one dedicated to testing develop...
Article
Full-text available
Phenyl valerate (PV) is a neutral substrate for measuring the PVase activity of neuropathy target esterase (NTE), a key molecular event of organophosphorus-induced delayed neuropathy. This substrate has been used to discriminate and identify other proteins with esterase activity and potential targets of organophosphorus (OP) binding. A protein with...
Poster
The official Guideline for testing developmental toxicity is the OECD TG 414. One single assay performed observing this OECD TG 414 requires several hundreds of rats or rabbits with a cost of around 100,000 €. There are no validated alternative method with regulatory acceptance for testing developmental toxicity, therefore requiring the development...
Poster
Introduction All regulations in developed countries require testing developmental toxicity according to the OECD TG 414, which involves the in vivo use of a high number of rats or rabbits with a high bioethical and economic impact. The possibilities for developmental toxicity assessment in vitro are quite limited. Indeed, only a few in vitro method...
Article
The official Guideline for testing developmental toxicity is the OECD TG 414. One single assay performed observing this OECD TG 414 requires several hundreds of rats or rabbits with a cost of around 100,000 €. There are no validated alternative method with regulatory acceptance for testing developmental toxicity, therefore requiring the development...
Article
O-hexyl O-2,5-dichlorophenyl phosphoramidate (HDCP) induces delayed neuropathy. The R(+)-HDCP inhibits and caused the so call “aging reaction” on inhibited-NTE. This enantiomer is not hydrolyzed by Ca(II)-dependent A-esterases in mammal tissues but is hydrolyzed by Cu(II)-dependent chicken serum albumin (CSA). With the aim of identifying HDCP hydro...
Article
Full-text available
Titanium dioxide and zinc oxide are two of the most widely used nanomaterials. We assessed the effects of noncytotoxic doses of both nanomaterials on T98G human glioblastoma cells by omic approaches. Surprisingly, no effects on the transcriptome of T98G cells was detected after exposure to 5 µg/mL of zinc oxide nanoparticles during 72 h. Conversely...
Article
Nanotechnology has been well developed in recent decades because it provides social progress and welfare. Consequently, exposure of population is increasing and further increases in the near future are forecasted. Therefore, assessing the safety of applications involving nanoparticles is strongly advisable. We assessed the effects of silver nanopar...
Chapter
DFP (diisopropylfluorophosphate) [(C3H7)2-P(O)-F] is a dialkyl fluorophosphate synthesized in the 1930s by procedures patented looking either for insecticides, mold control, warfare agents, or fluorine compounds for dental protection. It has been extensively used in research as a model compound of an acetylcholinesterase inhibitor and in other toxi...
Article
Full-text available
The Risk Assessment Committee of the European Chemical Agency released a scientific opinion alerting that the risk associated with dermal occupational exposure to bisphenol A (BPA) via thermal paper might not be adequately controlled because the estimated exposure was around twice the Derived No Effect Level (DNEL) and the European Commission will...
Article
The molecular targets of best known neurotoxic effects associated to acute exposure to organophosphorus compounds (OPs) are serine esterases located in the nervous system, although there are other less known neurotoxic adverse effects associated with chronic exposure to OPs whose toxicity targets are still not identified. In this work we studied se...
Article
Some effects of organophosphorus compounds (OPs) esters cannot be explained by action on currently recognized targets acetylcholinesterase or neuropathy target esterase (NTE). In previous studies, in membrane chicken brain fractions, four components (EPα, EPβ, EPγ and EPδ) of phenyl valerate esterase activity (PVase) had been kinetically discrimina...
Article
O-hexyl O-2,5-dichlorophenyl phosphoramidate (HDCP) is a chiral analogous compound of the methamidophos insecticide that induces delayed neuropathy, and the R-(+)-HDCP enantiomer is an inhibitor of neuropathy target esterase (NTE). This enantiomer is not hydrolized by Ca2+-dependent phosphotriesterases in mammal tissues. Our group had reported R-(+...
Chapter
The Organisation for Economic Cooperation and Development (OECD) has developed and validated guidelines for testing reproductive toxicity in vivo. These guidelines can be divided into two groups. One of these groups is devoted to testing only developmental toxicity and includes one guideline for testing general developmental toxicity and a second o...
Chapter
There are several methods alternative to animal experimentation for testing developmental toxicity. Among the array of available methods only three have overcome a blind validation study. These are the cellular methods called embryonic stem cell tests and micromass test and the test with embryos called whole embryo culture test. Other nonvalidated...
Article
Avian species contain low levels of enzymes that hydrolyze organophosphorus compounds (OPs), and chickens are used as a model of OPs delayed neurotoxicity. For both reasons, we studied the ability of chicken tissue for OP detoxication. A significant activating effect of Cu²⁺ on the hydrolysis of O-hexyl O-2,5-dichlorophenyl phosphoramidate (HDCP) w...
Poster
Organophosphorus compounds (OPs) induce neurotoxic disorders through interactions with well-known target esterases, such as acetylcholinesterase and neuropathy target esterase (NTE).However, OPs interact with other esterases of unknown biological function. Our aim is the identification of new targets of OP toxicity. In membrane chicken brain fracti...
Article
Some effects of organophosphorus compounds (OPs) esters cannot be explained through actions on currently recognized targets acetylcholinesterase or neuropathy target esterase (NTE). In soluble chicken brain fraction, three components (Eα, Eβ and Eγ) of pheny lvalerate esterase activity (PVase) were kinetically discriminated and their relationship w...
Article
Neuropathy Target Esterase (NTE) is a membrane protein codified by gene PNPLA6. NTE was initially discovered as a target of the so-called organophosphorus-induced delayed polyneuropathy triggered by the inhibition of the NTE-associated esterase center by neuropathic organophosphorus compounds (OPs). The physiological role of NTE might be related to...
Chapter
When assessing the air quality in textile and related industries, one of the most important issues to be considered is the exposure to chemicals substances, as it might represent a health concern in industry and is subject to different airborne chemical occupational limits. This chapter attempts to describe the factors which might have an impact in...
Article
Chlorpyrifos (CPS) is an organophosphorus compound (OP) capable of causing well-known cholinergic and delayed syndromes through the inhibition of acetylcholinesterase and Neuropathy Target Esterase (NTE), respectively. CPS is also able to induce neurodevelopmental toxicity in animals. NTE is codified by the Pnpla6 gene and plays a central role in d...
Chapter
DFP (diisopropylfluorophosphate; (C3H7)2-P(O)-F) is a dialkylfluorophosphate synthesized in the 1930s by procedures patented for insecticides, mold control, warfare agents, and fluorine compounds for dental protection. However, DFP has never been produced for chemical warfare and is not used as an insecticide. It has been extensively used in resear...
Article
Full-text available
There is a necessity to develop in vitro methods for testing embryotoxicity (Romero et al., 2015) [1]. We studied the progress of D3 mouse embryonic stem cells differentiation exposed to model embryotoxicants and non-embryotoxicants chemicals through the expression of biomarker genes. We studied a set of 16 different genes biomarkers of general cel...
Article
Embryonic Stem cell Test (EST) is an in vitro validated assay for testing embryotoxicity.. The EST needs improvements before being used for regulatory purposes, but also needs technical simplification for use in high throughput screenings. We propose the quantification in alterations of the differentiation of D3 monolayer cells cultures through the...
Article
Petroleum distillates are mixtures of C7–C12 hydrocarbons with 30–50% of linear and branched alkanes, 30–40% of cycloalkanes, and up to 25% of aromatics. Their main uses are in extraction, cleaning, and as degreasing solvents. The most relevant route of exposure is inhalation. Petroleum distillates can be bioaccumulated in either adipose tissue or...
Article
The term ‘lubricating oil’ refers a wide range of products that are characterized by hundreds of base chemicals and additives. Lubricating oils may be either mineral-based or synthetic. This article focuses on petroleum-based lubricating oils, which are composed of 80–90% of petroleum hydrocarbon distillates and 10–20% of additives and are used for...
Article
Full-text available
Many cholinesterase assays are performed to study the inhibition of cholinesterase (ChE) activity. Frequently a large number of samples are processed and Ellman's method [1] is the most commonly used [2,3]. Activity is estimated from the increment in absorbance between two reaction times when the reaction is not stopped. Bellino et al. [4] describe...
Article
Neuropathy Target Esterase (NTE) is a protein involved in the development of a polyneuropathy caused by exposure to certain organophosphorus compounds. In vivo and in vitro studies have also associated NTE with embryonic development since NTE null mice embryos are non-viable, and silencing the NTE-codifying gene (Pnpla6) in mouse embryonic stem cel...
Article
There are discrepancies about whether chlorpyrifos is able to induce neurodevelopmental toxicity or not. We previously reported alterations in the pattern of expression of biomarker genes of differentiation in D3 mouse embryonic stem cells caused by chlorpyrifos and its metabolites chlorpyrifos-oxon and 3,5,6-trichloro-2-pyridinol. Now, we reanalyz...
Article
For every kilogram of saffron spice produced, about 63kg of floral bio-residues (FB) (tepals, stamens and styles) are thrown away. Extracts of these bio-residues in water (W1), water:HCl (100:1, v/v) (W2), ethanol (E3), ethanol:HCl (100:1, v/v) (E4), dichloromethane (D5) and hexane (H6) were prepared. Their composition in flavonols and anthocyanins...
Article
Biomarkers yield information as to whether an organism has been exposed to a xenobiotic (biomarkers of exposure), about the response of the organism exposed to the xenobiotic (biomarkers of effect), or about the susceptibility of the organism to xenobiotics. Biomarkers of exposure can be used to biomonitor xenobiotic exposure, even after the organi...
Article
Organophosphorus compounds (OPs) induce neurotoxic disorders through interactions with well-known target esterases, such as acetylcholinesterase and neuropathy target esterase (NTE). However, OPs interact with other esterases of unknown biological function. In soluble chicken brain fractions, three components of enzymatic phenylvalerate esterase ac...
Article
Full-text available
Historically, only few chemicals have been identified as neurodevelopmental toxicants, however, concern remains, and has recently increased, based upon the association between chemical exposures and increased developmental disorders. Diminution in motor speed and latency has been reported in preschool children from agricultural communities. Organop...
Article
Full-text available
Neuropathy target esterase (NTE) is involved in several disorders in adult organisms and embryos. A relationship between NTE and nervous system integrity and maintenance in adult systems has been suggested. NTE-related motor neuron disease is associated with the expression of a mutant form of NTE and the inhibition and further modification of NTE b...
Article
Full-text available
The goal of coaxial methods is to direct pluripotent stem cells toward specific phenotypes, limiting the appearance of tumorigenic or apoptotic cells. Beside culture medium components, other parameters, such as incubator atmosphere composition, temperature or pH of the culture medium, may have an influence on culture progression. In this study, the...
Article
The effects of organophosphate insecticide chlorpyrifos (CPF) on development is currently under discussion. CPF and its metabolites, chlorpyrifos-oxon (CPO) and 3,5,6-trichloro-2-pyridinol (TClP) were more cytotoxic for D3 mouse embryonic stem cells than for differentiated fibroblasts 3T3 cells. Exposure to 10μM CPF and TClP and 100μM CPO for 12hou...
Article
Organophosphorus compounds (OPs) cause neurotoxic disorders through interactions with well-known target esterases, such as acetylcholinesterase and neuropathy target esterase (NTE). However,the OPs can potentially interact with other esterases of unknown significance. Therefore, identifying, characterizing and elucidating the nature and functional...
Article
This study measured inhalation exposure to 13 volatile organic compounds (VOCs) among workers in the leatherwear industry in Spain, examined the changes in those exposures over a 5-year period, and documented local exhaust ventilation practices that affected exposure. In collaboration with an occupational risk prevention company, air samples were c...
Article
This chapter discusses the methods adapted by the OECD and some other protocols, including the general principles of the study, the main aspects of the procedure, the endpoints and the observations, data reporting and criteria for interpreting results, and summarizing the guidelines. OECD 414 provides general information concerning the effects of p...
Article
This chapter discusses the study of the currently available models for testing developmental toxicity (embryotoxicity and teratogenicity). The main alternative models for testing developmental toxicity are described. These models are divided between validated models (whole-embryo culture test (WEC), micromass test (MM) and embryonic stem cell test...
Chapter
IntroductionDetoxication of Organophosphorus PesticidesDetoxication of Carbamate PesticidesConclusions References
Article
Full-text available
The embryonic Stem cell Test (EST) is a validated assay for testing embryotoxicity in vitro. The total duration of this protocol is 10 days, and its main end-point is based on histological determinations. It is suggested that improvements on EST must be focused toward molecular end-points and, if possible, to reduce the total assay duration. Five d...
Article
Full-text available
Neuropathy Target Esterase (NTE) was initially identified as the primary target esterase of some organophosphorus compounds that cause delayed neuropathy. Some studies in vivo suggest that this protein may also perform a function in embryonic development and therefore also in cell differentiation. The aim of this work was to characterize embryonic...
Article
Full-text available
Embryonic stem (ES) cells are considered an important alternative to develop in vitro screening methods for embryotoxicity. Mouse ES cells can be cultured as cell suspension aggregates termed "embryoid bodies" (EBs) in which cells start to differentiate. We have studied the expression of several genes in the presence of a wide range of concentratio...
Article
Organophosphorus-induced delayed polyneuropathy (OPIDP) is a syndrome induced by certain organophosphorus compounds (OPs) through a mechanism based on the inhibition and further modification (aging) of neuropathy target esterase (NTE). OECD guidelines for testing the capability of OPs to trigger OPIDP include two in vivo tests with hens. Activities...
Article
Serum albumin displays an esterase activity that is capable of hydrolysing the anti-cholinesterase compounds carbaryl, paraoxon, chlorpyrifos-oxon, diazoxon and O-hexyl, O-2,5-dichlorphenyl phosphoramidate. The detoxication of all these anti-cholinesterase compounds takes place at significant rates with substrate concentrations in the same order of...
Article
Human serum albumin was able to hydrolyze the organophosphorus compounds paraoxon, chlorpyrifos-oxon, and diazoxon at toxicologically relevant concentrations. Human serum displayed two paraoxon hydrolyzing activities: the so-called paraoxonase, which is associated with the lipoprotein fraction and is calcium dependent and EDTA sensitive, and the ac...
Article
The Ca(2+)-dependent and EDTA-resistant hydrolysis of O-hexyl O-2,5-dichlorophenyl phosphoramidate (HDCP) and paraoxon was studied in serum and subcellular fractions of liver, kidney and brain of hen, rat and rabbit. HDCP was the best substrate among all the tissues studied, except that of rabbit serum which showed the highest Ca(2+)-dependent para...