Miguel Larguinho

Karolinska Institutet | KI · Department of Cellular and Molecular Biology

Propose: Tin complexes demonstrate antiproliferative activities in some case higher than cisplatin, with IC50 at the low micromolar range. We have previously showed that the cyclic trinuclear complex of Sn(IV) bearing an aromatic oximehydroxamic acid group [nBu2Sn(L)]3 (L=N,2-dihydroxy-5-[N-hydroxyethanimidoyl]benzamide) (MG85) shows high anti-pro...

Nanotechnology has led to the development of new and improved materials, and particular emphasis has been directed toward nanoparticles and their multiple bio-applications. Nanoparticles exhibit size-, shape-, and composition-dependent properties, e.g., surface plasmon resonance and photothermal properties, which may potentially enhance laser desor...

Non-crosslinking (NCL) approaches using DNA-modified gold nanoparticles for molecular detection constitute powerful tools with potential implications in clinical diagnostics and tailored medicine. From detection of pathogenic agents to identification of specific point mutations associated with health conditions, these methods have shown remarkable...

Several copper complexes have been assessed as anti-tumor agents against cancer cells. In this work, a copper compound [Cu(H2O){OS(CH3)2}L](NO3)2 incorporating the ligand 4'-phenyl-terpyridine antiproliferative activity against human colorectal, hepatocellular carcinomas and breast adenocarcinoma cell lines was determined, demonstrating high cytoto...

Frontiers in Nanomedicine offers an up-to-date understanding of nanomaterials to readers having clinical or biomolecular research interests. Scientists, both aspiring and experienced, will find, in each volume, a comprehensive overview of current molecular strategies for using nanoscale materials in medicine. This volume explains the use of nanotec...

The main objectives of the present study were to assess the vulnerability of the gilthead sea bream (Sparus aurata) larvae to temperature increase contributing to the understanding of the susceptibility of this species to temperature changes, heat waves, and potentially climate warming. Thus, several biochemical thermal stress responses were assess...

Acrylamide is an amide used in several industrial applications making it easily discharged to aquatic ecosystems. The toxicity of acrylamide to aquatic organisms is scarcely known, although previous studies with murine models provided evidence for deleterious effects. To assess the effects of acrylamide to freshwater fish, goldfish (Carassius aurat...

Abstract Antisense therapy is a powerful tool for post-transcriptional gene silencing suitable for downregulating target genes associated to disease. Gold nanoparticles (AuNPs) have been described as effective intracellular delivery vehicles for antisense oligonucleotides providing increased protection against nucleases and targeting capability via...

This work reports a one-way flow bioaccumulation of gold nanoparticles (AuNPs) in aquatic organisms between two trophic levels. First, Dunaliella salina cells were exposed to citrate-capped AuNPs at different concentrations and during distinct exposure periods to assess internalization and behavior. Afterward, D. salina was incubated with both citr...

The optical and physico-chemical properties of gold nanoparticles (AuNPs) have prompted new and improved approaches which have greatly evolved the fields of biosensing and molecular detection. In this study, the authors took advantage of AuNPs' ease of modification and functionalised it with selected DNA aptamers using a salt aging method to produc...

The very high antiproliferative activity of [Co(Cl)(H2O)(phendione)2][BF4] (phendione is 1,10-phenanthroline-5,6-dione) against three human tumor cell lines (half-maximal inhibitory concentration below 1 μM) and its slight selectivity for the colorectal tumor cell line compared with healthy human fibroblasts led us to explore the mechanisms of acti...

Gold nanoparticles (AuNPs) have been widely applied as intracellular delivery vehicles for post-transcriptional gene regulation using antisense oligonucleotides due to their unique properties, such as ease of synthesis and functionalisation with multiple targets. Besides this, AuNPs provide protection against intracellular nucleases and do not requ...

We report a method centred on gold nanoparticle-based surface-assisted laser desorption/ionisation for analysis of deoxynucleotides and alkylated nucleobases. Gold nanoparticles allow for enhanced analysis capability by eliminating undesired signature peaks; thus more elegant mass spectra can be attained that allow identification by nucleotide mass...

Nanotechnology has prompted researchers to develop new and improved materials aimed at biomedical applications with particular emphasis in diagnostics and therapy. Special interest has been directed at providing enhanced biomolecular diagnostics, including SNP detection gene expression profiles and biomarker characterisation. These strategies have...

Two ligands, L-1 and L-2, derived from 2,3-dihydroxybenzaldehyde and 8-hydroxyquinoline-2-carbaldehyde using 4,4'-methylene-dianiline as spacer have been used to study their interaction with the group 13 metals, Al3+, Ga3+ and In3+. In all cases, emissive dinuclear metal complexes were synthesized and characterized. The complexation reactions with...

Gold nanoparticles (AuNPs) have been extensively used for the development of new molecular diagnostic assays and biomedical applications due to their unique optical properties. These optical properties are derived from the characteristic surface plasmon resonance (SPR) band that can be modulated - enhanced and easily tailored - through the synthesi...

A specific colorimetric DNA detection method based on oligonucleotide functionalized gold–silver-alloy nanoparticles (AuAg-alloy-nanoprobes) is presented. The AuAg-alloy-nanoprobes were then used for the specific detection of a DNA sequence from TP53—a gene involved in cancer development. The AuAg-alloy-nanoprobes were then used in combination with...

Several ultrasound-based platforms for DNA sample preparation were evaluated in terms of effective fragmentation of DNA (plasmid and genomic DNA)-ultrasonic probe, sonoreactor, ultrasonic bath and the newest Vialtweeter device. The sonoreactor showed the best efficiency of DNA fragmentation while simultaneously assuring no cross-contamination of sa...

Due to their unique optical properties, noble metal nanoparticles, and in particular gold nanoparticles (AuNPs), have been extensively used for the development of new molecular diagnostic assays[1]. These optical properties derive from the characteristic surface plasmon resonance (SPR) band that can be easily tailored through the synthesis of NPs w...