• Home
  • Miguel Angel Martin-Piedra
Miguel Angel Martin-Piedra

Miguel Angel Martin-Piedra
University of Granada School of Medicine · Department of Histology

DDS, PhD in Biomedicine
Lecturer in Histology - University of Granada

About

66
Publications
14,929
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
586
Citations
Citations since 2016
44 Research Items
502 Citations
2016201720182019202020212022020406080100
2016201720182019202020212022020406080100
2016201720182019202020212022020406080100
2016201720182019202020212022020406080100
Introduction
Miguel Angel Martin-Piedra currently works at the Department of Histology, University of Granada. Miguel Angel does research in Stem cells, Artificial tissues, Tissue Engineering, Histology and Molecular Biology. Their current project is 'Artificial tissues by Tissue engineering and medical applications'.

Publications

Publications (66)
Article
Full-text available
Advances in skin tissue engineering have promoted the development of artificial skin substitutes to treat large burns and other major skin loss conditions. However, one of the main drawbacks to bioengineered skin is the need to obtain a large amount of viable epithelial cells in short periods of time, making the skin biofabrication process challeng...
Article
Full-text available
Translational medicine is an important area of biomedicine, and has significantly facilitated the development of biomedical research. Despite its relevance, there is no consensus on how to evaluate its progress and impact. A systematic review was carried out to identify all the methods to evaluate translational research. Seven methods were found ac...
Article
Students’ metacognitive skills and perceptions are considered important variables for high‐quality learning. In this study, students’ perceptions were used to identify histological threshold concepts (integrative, irreversible, transformative and troublesome) in three health sciences curricula. A specific questionnaire was developed and validated t...
Article
Full-text available
Introduction Immediate Life Support training is under-represented in medical degrees and little information is available on the medium-term effectiveness of its teaching. Our aim was to analyse the forgetting curve in medical students who received specific training in the three main procedural competencies of Immediate Life Support. Material and m...
Article
Full-text available
Background: Tissue engineering (TE) constitutes a multidisciplinary field aiming to construct artificial tissues to regenerate end-stage organs. Its development has taken place since the last decade of the 20th century, entailing a clinical revolution. TE research groups have worked and shared relevant information in the mass media era. Thus, it wo...
Article
Full-text available
Critical defects of the mandibular bone are very difficult to manage with currently available materials and technology. In the present work, we generated acellular and cellular substitutes for human bone by tissue engineering using nanostructured fibrin–agarose biomaterials, with and without adipose-tissue-derived mesenchymal stem cells differentia...
Article
Full-text available
Objective The aim of this study was to generate novel models of bioartificial human oral mucosa with increased vascularization potential for future use as an advanced therapies medicinal product, by using different vascular and mesenchymal stem cell sources. Background Oral mucosa substitutes could contribute to the clinical treatment of complex d...
Article
Full-text available
Genomic analysis and digitalization of medical records have led to a big data scenario within hematopathology. Artificial intelligence and machine learning tools are increasingly used to integrate clinical, histopathological, and genomic data in lymphoid neoplasms. In this study, we identified global trends, cognitive, and social framework of this...
Article
Full-text available
La Parada Cardiorrespiratoria (PCR) es un problema de salud pública en los países desarrollados. De acuerdo con La Sociedad Española de Resucitación, en España hay alrededor de treinta mil muertes al año y unos veinte mil intentos de resucitación, y aun así hasta hace muy poco los alumnos de Medicina no recibían formación reglada en Soporte Vital A...
Preprint
BACKGROUND Tissue engineering (TE) constitutes a multidisciplinary field aiming to construct artificial tissues to regenerate end-staged organs. Its development has taken placed since the last decade of the 20th century, entailing a clinical revolution. In this sense, TE research groups have worked and shared relevant information in the mass media...
Preprint
BACKGROUND Tissue engineering (TE) constitutes a multidisciplinary field aiming to construct artificial tissues to regenerate end-staged organs. Its development has taken placed since the last decade of the 20th century, entailing a clinical revolution. In this sense, TE research groups have worked and shared relevant information in the mass media...
Preprint
Full-text available
Background Tissue engineering (TE) constitutes a multidisciplinary field aiming to construct artificial tissues to regenerate end-stage organs. Its development has taken place since the last decade of the 20th century, entailing a clinical revolution. TE research groups have worked and shared relevant information in the mass media era. Thus, it wou...
Article
During the last XX century, several changes were applied to traditional educational methods, positioning the student as a central actor in the learning process. One of the pedagogical theories developed was the Threshold Concepts (TC) educational framework, based on education as a space of uncertainty, where the student needs to learn a certain con...
Article
Full-text available
PurposeHuman cornea substitutes generated by tissue engineering currently require limbal stem cells for the generation of orthotypical epithelial cell cultures. We recently reported that bioengineered corneas can be fabricated in vitro from a heterotypical source obtained from Wharton’s jelly in the human umbilical cord (HWJSC).Methods Here, we gen...
Article
In this work, we have analyzed the main clinical and corneal histological parameters that may be associated to the spherical equivalent (SE), age and gender of individuals with myopic refractive errors. For this purpose, 108 cornea stroma lenticules were obtained from patients subjected to ReLEx-SMILE myopia correction. Histological analyses were c...
Article
Full-text available
Recent advances in tissue engineering offer innovative clinical alternatives in dentistry and regenerative medicine. Tissue engineering combines human cells with compatible biomaterials to induce tissue regeneration. Shortening the fabrication time of biomaterials used in tissue engineering will contribute to treatment improvement, and biomaterial...
Article
Human Wharton's jelly stem cells (HWJSC) can be efficiently isolated from the umbilical cord, and numerous reports have demonstrated that these cells can differentiate into several cell lineages. This fact, coupled with the high proliferation potential of HWJSC, make them a promising source of stem cells for use in tissue engineering and regenerati...
Article
Publication numbers have been widely used as measure of research output, especially academic and university research. Publication numbers in tissue engineering (TE) have increased year by year since early 1990s. However, after an exponential growth phase, recently publications increase at lower rates, suggesting a consolidation process in which rev...
Article
Full-text available
RESUMEN En este trabajo se ha realizado una revisión bibliográfica de los artículos más relevantes que han sido publicados en revistas científicas de impacto durante los últimos años con el objetivo de conocer las nuevas opciones terapéuticas en el tratamiento de las complicaciones fistulosas de la Enfermedad de Crohn que repercuten de forma import...
Article
Full-text available
Background: Advanced therapies are increasingly demanded by patients with the intent of treating some incurable conditions. Because family medicine professionals play an important role as health educators, their residency programs should incorporate new knowledge related to advanced therapies. To successfully implement these programs, how family m...
Data
Raw data showing the scores assigned by each resident to each item of the questionnaire. (XLSX)
Data
Questionnaire used in the present study, with the different components, topics, items and abbreviated item names used in the tables. (PDF)
Article
Full-text available
Mesenchymal stem cells (MSCs) can differentiate toward epithelial cells and may be used as an alternative source for generation of heterotypical artificial human skin substitutes, thus, enhancing their development and translation potential to the clinic. The present study aimed at comparing four types of heterotypical human bioengineered skin gener...
Article
Full-text available
The structure of the human skin is directly dependent on its location and the mechanical forces to which it is subjected. In the present work, we have performed a comprehensive analysis of the human ridged and non-ridged skin to identify the differences and similarities between both skin types. For this purpose, human skin samples were obtained fro...
Article
Impact statement: This study evaluates the cognitive structure and social behavior of tissue engineering (TE) based on a science mapping analysis. Understanding the terms and topics that play a key role in the development of TE can help administrative authorities to better plan funding. Moreover, a better knowledge of collaborative networks in TE...
Article
Full-text available
Background: The students' conceptions of learning in postgraduate health science master studies are poorly understood. The aim of this study was to compare the factors influencing conceptions of learning in health sciences and non-health sciences students enrolled in postgraduate master programs in order to obtain information that may be useful fo...
Article
Tissue engineering is defined as a multidisciplinary scientific discipline with the main objective to develop artificial bioengineered living tissues in order to regenerate damaged or lost tissues. Since its appearance in 1988, tissue engineering has globally spreaded in order to improve current therapeutical approaches, entailing a revolution in c...
Article
Full-text available
Purpose: The objective of the present work is the development of a new protocol for the inclusion of paraffin-embedded agarose gels to allow their study in histology and the subsequent characterization of two different types of agarose, Type I and Type VII, for their use in Tissue Engineering.Method: Different protocols were evaluated for a correct...
Article
Background: Repair of abdominal wall defects is one of the major clinical challenges in abdominal surgery. Most biomaterials are associated to infection and severe complications, making necessary safer and more biocompatible approaches. Aim: In the present work, we combined the adequate mechanical properties of synthetic polymer meshes with tiss...
Article
As a joint University-Healthcare System effort, we have developed a technology that allows the generation of artificial biological tissues from fibrin–agarose biomaterials and various cell types. The Tissue Engineering Unit of University of Granada started basic R&D back in 2006, and after patent filing, preclinical scientific advice, preclinical r...
Article
Cell-derived matrices were recently described as novel biomaterials generated by human cells allowed to grow and synthetize their own extracellular matrix in culture. In the present work, we generated and evaluated a novel tissue-like substitute (WDM) consisting of a membrane derived from cultured human Wharton's jelly stem cells. WDM were evaluate...
Article
Full-text available
Current tissue engineering technology focuses on developing simple tissues, whereas multilayered structures comprising several tissue types have rarely been described. We developed a highly biomimetic multilayered palate substitute with bone and oral mucosa tissues using rabbit cells and biomaterials subjected to nanotechnological techniques based...
Article
We evaluated the efficiency of several protocols to preserve the main components of decellularized tissue scaffolds for delayed use. Decellularized rat intestine scaffolds were generated by using SDS and triton X-100 and preserved for 3 months subjected to eight freeze-drying (F1 to F8) and 14 cryopreservation protocols (C1 to C14). Morphological a...
Article
Regeneration of the pulp-dentin complex with stem cells is a potential alternative to conventional root canal treatments. Human dental pulp stem cells (hDPSCs) have been extensively studied because of their ability to proliferate and differentiate into mineralized dental and nondental tissues. In this work we combined hDPSCs with two types of injec...
Article
Full-text available
The use of mucoperiostial flaps during cleft palate surgery is associated with altered palatal bone growth and development. We analyzed the potential usefulness of a bioengineered oral mucosa in an in vivo model of cleft palate. First, a 4 mm palate defect was created in one side of the palate oral mucosa of 3 week-old New Zealand rabbits, and a co...
Article
We carried out an in vivo study to evaluate the potential usefulness of a novel bioengineered bone substitute for the repair of palate defects in laboratory rabbits, using tissue-engineering methods. Our results showed that the use of a bioengineered bone substitute was associated with more symmetrical palate growth as compared to the controls, and...
Article
Full-text available
We evaluated the cytotoxic effects of four prostaglandin analogs (PGAs) used to treat glau-coma. First we established primary cultures of conjunctival stromal cells from healthy donors. Then cell cultures were incubated with different concentrations (0, 0.1, 1, 5, 25, 50 and 100%) of commercial formulations of bimatoprost, tafluprost, travoprost an...
Article
Oral mucosa shortage may limit or condition some clinical approaches in maxillofacial, periodontal and implant treatment. The availability of a human oral mucosa model generated by tissue engineering could help clinicians to address the lack of oral mucosa. In this work, we carried out a sequential maturation and differentiation study of the epithe...
Article
Full-text available
Dupuytren's disease is a fibro-proliferative disease characterized by a disorder of the extracellular matrix (ECM) and high myofibroblast proliferation. However, studies failed to determine if the whole palm fascia is affected by the disease. The objective of this study was to analyze several components of the extracellular matrix of three types of...
Article
Introduction: Human umbilical cord stem cells have inherent differentiation capabilities and potential usefulness in regenerative medicine. However, the epithelial differentiation capability and the heterogeneity of these cells have not been fully explored to the date. Methods: We analyzed the expression of several undifferentiation and epitheli...
Article
Purpose: Human Wharton's jelly stem cells (HWJSCs) are able to differentiate into skin and oral mucosa epithelial-like cells. In this work, we demonstrate for the first time the capability of HWJSCs to differentiate in vitro into cornea epithelial-like cells in a three-dimensional model. Methods: First, primary cell cultures of HWJSCs, corneal e...
Article
The students' motivation to learn basic sciences in health science curricula is poorly understood. The purpose of this study was to investigate the influence of different components of motivation (intrinsic motivation, self-determination, self-efficacy and extrinsic -career and grade- motivation) on learning human histology in health science curric...
Article
Evaluation of cell viability is one of the most important steps of the quality control process for therapeutic use of cells. The aim of this study was to evaluate the long-term cell viability profile of human dental pulp stem cell (hDPSC) subcultures (beyond 10 passages) to determine which of these passages are suitable for clinical use and to iden...
Article
Full-text available
Local anesthetic drugs are extensively used in dentistry. However, the cytotoxic effects of these pharmaceutical compounds remain unclear. In this work, we have evaluated the cell viability and cell function of human oral mucosa fibroblasts exposed to different concentrations of lidocaine for increasing incubation times, using a global screening me...
Article
The purpose of this study was to determine the histological and functional (immunohistochemical) changes that take place in oral mucosa grafts implanted in the rat urethra. Urethroplasty was performed in 26 male Wistar rats weighing 250g. All animals received autologous oral mucosa urethra grafting under general anesthesia. Samples were analyzed 10...
Article
Background aims: One of the most important issues in tissue engineering (TE) is the search for a suitable stem cell reservoir with optimal cell viability levels for the development of new tissues relevant for therapeutic needs. The aim of this study was to evaluate the cell viability levels of 10 sequential cell passages of human dental pulp stem...
Article
Two questionnaires were used to investigate students' perceptions of their motivation to opt for reception learning (RL) or self-discovery learning (SDL) in histology and their choices of complementary learning strategies (CLS). The results demonstrated that the motivation to attend RL sessions was higher than the motivation to attend SDL to gain n...
Article
Human umbilical cord Wharton's jelly stem cells (HWJSCs) are gaining attention as a possible clinical source of mesenchymal stem cells for cell therapy and tissue engineering due to their high accessibility, expansion potential, and plasticity. We employed a combination of highly sensitive techniques to determine the average cell viability levels a...
Article
Full-text available
Objectives: Use of certain drugs is the most common aetiology of xerostomia. Systemic sialogogues provide a longer effect than topic ones, but also induce relevant side effects. Topical sialogogues, as malic acid, allow a safe use as they induce no systemic side-effects or pharmacological interactions, being especially interesting in cases of mild...
Conference Paper
OBJECTIVES: The aim of this experimental study is to assess the properties of two xenografts (porcine vs bovine) as biomaterials for osseous tissue replacement, due to its high relevance in oral and maxillofacial surgery. Xenografts are followed-up for two months in order to compare them in terms of biocompatibility, resorption and osseous behaviou...
Conference Paper
OBJECTIVES: The aim of this study is to review those papers describing experimental design in order to evaluate the efficacy and feasibility of acupuncture as a valid treatment option for xerostomia, as a consequence from either head-and-neck radiotherapy, or systemic diseases or other aethiologies (drugs, depressive disorders,). METHODS: A bibli...
Article
Full-text available
Treatment in patients with atrial fibrillation or venous thromboembolism in recent decades has been based almost exclusively on the use of vitamin K antagonists. These drugs have a narrow therapeutic index, so it is precise to repeated adjustments of doses that require analytical monitoring. For many years it has advocated the need to have more con...