Miguel Angel García-Campos

Weizmann Institute of Science | weizmann · Department of Molecular Genetics

A bstract We present txtools, an R package that enables the processing, analysis, and visualization of RNA-seq data at the nucleotide-level resolution, seamlessly integrating alignments to the genome with transcriptomic representation. txtools’ main inputs are BAM files and a transcriptome annotation, and the main output is a table, capturing misma...

N⁶-methyladenosine (m6A) is the most prevalent modification of messenger RNA in mammals. To interrogate its functions and dynamics, there is a critical need to quantify m6A at three levels: site, gene and sample. Current approaches address these needs in a limited manner. Here we develop m6A-seq2, relying on multiplexed m6A-immunoprecipitation of b...

The function of catalases A and T from the budding yeast Saccharomyces cerevisiae (ScCta1 and ScCtt1) is to decompose hydrogen peroxide (H2O2) to mitigate oxidative stress. Catalase orthologs are widely found in yeast, suggesting that scavenging H2O2 is crucial to avoid the oxidative damage caused by reactive oxygen species (ROS). However, the func...

N6-methyladenosine (m6A) is the most abundant modification on mRNA and is implicated in critical roles in development, physiology, and disease. A major limitation has been the inability to quantify m6A stoichiometry and the lack of antibody-independent methodologies for interrogating m6A. Here, we develop MAZTER-seq for systematic quantitative prof...

N6-methyladenosine (m⁶A) is the most abundant modification on mRNA, and is implicated in critical roles in development, physiology and disease. The ability to map m⁶A using immunoprecipitation-based approaches has played a critical role in dissecting m⁶A functions and mechanisms of action. Yet, these approaches are of limited specificity, unknown s...

Pathway analysis is a set of widely used tools for research in life sciences intended to give meaning to high-throughput biological data. The methodology of these tools settles in the gathering and usage of knowledge that comprise biomolecular functioning, coupled with statistical testing and other algorithms. Despite their wide employment, pathway...

Debaryomyces hansenii es una levadura aislada por primera vez del mar, pero presente en diversos hábitats naturales y creados por el hombre. Estos en su mayoría presentan baja actividad acuosa, altos contenidos de sales y temperaturas bajas. Por lo que D. hansenii ha tenido que desarrollar sistemas celulares de resistencia ante tales tipos de estré...

Heterologous complemented strains Sc acat::DCTTA and Sc acat::DhCTT showed to be more resistant to oxidative stress, mediated by H2O2, than the wild type strain of S. cerevisiae. This advantage was more evident in presence of salt (0.6 M), this evidence added to an analysis of the amino acid composition of D. hansenii’s catalases compared to S. ce...