Michihiro Matsuki

Aim: To compare the clinical usefulness of once-weekly glucagon-like peptide-1 receptor agonists dulaglutide and semaglutide at the doses approved for use in Japanese patients with type 2 diabetes. Methods: In total, 120 patients with glycated haemoglobin (HbA1c) ≥7% were randomly assigned to dulaglutide (n = 59) or semaglutide group (n = 61), a...

Objective To examine the association between glycemic control and the new onset of macroangiopathy in Japanese subjects with type 2 diabetes. Methods We examined seven-year follow-up data for 572 patients. We divided the subjects by the average of seven-year glycemic control based on the guidelines. First, we excluded the subjects with a past histo...

A 71-year-old woman with type 2 diabetes was referred to our hospital because of severe lipoatrophy throughout the whole abdomen induced by insulin therapy. The patient was diagnosed with type 2 diabetes when she was 63 years old and was treated with diet therapy only. When she was 69, her glycemic control became poor and insulin therapy was introd...

To evaluate the efficacy of azelnidipine and amlodipine on diabetic nephropathy and atherosclerosis, we designed a prospective and randomized controlled clinical study in type 2 diabetic patients with stable glycemic control with fixed dose of anti-diabetic medication. Although there was no difference in blood pressure between both groups, urinary...

The survival of patients infected with human immunodeficiency virus (HIV) has been dramatically improved after the introduction of highly active antiretroviral therapy (HAART), the combination of multi-antiretroviral agents to decrease the replication of HIV type 1 (HIV-1). Although HIV-infected patients commonly show a low CD4-positive lymphocyte...

Aim: To evaluate the efficacy of miglitol and mitiglinide alone or in combination on the metabolic profile and incretin secretion in Japanese type 2 diabetes patients. Methods: Patients on diet and exercise with or without metformin, were randomized to receive either miglitol, mitiglinide, or a combination, three times daily for 12 weeks. Resul...

Dipeptidyl peptidase4 inhibitors, first approved in Japan in 2009, are rapidly expanding as one of the useful therapeutic options for patients with type 2 diabetes mellitus. However, the fact that DPP4 is identical to CD 26, a cell surface glycoprotein expressed by a variety of cells, including T cells, B cells, NK cells and macrophages, had long b...

To assess the clinical usefulness of insulin detemir, we observed HbAlc, BMI, insulin dose, and hypoglycemia frequency during the 6 months after subjects with type 2 diabetes switched from insulin NPH to insulin detemir with intensive insulin therapy. In 63 subjects, the daily insulin detemir dose increased significantly at 6 months compared to bas...

The ankle-brachial index (ABI) estimated in diagnosing peripheral arterial disease (PAD) often does not reflect PAD severity in arterial medium calcification. To assess the toe-brachial index (TBI) on PAD diagnosis in subjects with type 2 diabetes (T2DM), the ABI and TBI were measured and the relationship assessed with clinical parameters in 74 sub...

We investigated the molecular mechanism by which the human glucagon-like peptide-1 analogue liraglutide preserves pancreatic beta cells in diabetic db/db mice. Male db/db and m/m mice aged 10 weeks received liraglutide or vehicle for 2 days or 2 weeks. In addition to morphological and biochemical analysis of pancreatic islets, gene expression profi...

Pioglitazone preserves pancreatic beta-cell morphology and function in diabetic animal models. In this study, we investigated the molecular mechanisms by which pioglitazone protects beta-cells in diabetic db/db mice. In addition to the morphological analysis of the islets, gene expression profiles of the pancreatic islet were analyzed using laser c...

The db gene homozygous, but not heterozygous, mice develop diabetes with severe beta-cell damage. We investigated the molecular mechanism underlying db gene-associated pancreatic beta-cell dysfunction. Islet morphology, beta-cell function, and gene expression profiles specific for pancreatic islet cells were compared among db gene homozygous(db/db)...

We first discovered autoantibodies to ACE (angiotensin converting enzyme) in patients with type 2 diabetes mellitus^1^. The antibodies were positive for 64.5% of the patients with diabetes and were positive in 83.3% in the early stage of clinical diabetic nephropathy. In addition, in genetically diabetogenic OLETF (Otsuka Long-Evans Tokushima Fatty...

A 29-year-old woman was diagnosed as having type 1 diabetes mellitus and received insulin aspart and NPH insulin (NovolinN). On day 22, she had leg edema and right abdominal pain. The serum hepatobiliary enzyme levels were markedly elevated. Computed tomography revealed gallbladder edema. After an injection of human regular insulin and NPH insulin...

MIN6 cells retains glucose-stimulated insulin secretion (GSIS) as isolated islets. We comprehensively evaluated the gene expression and production of other islet hormones in MIN6 cells. Islet hormones were demonstrated by immunohistochemical staining and measured by ELISA. The gene expression profiles of MIN6 cells were compared with those in the m...

Adiponectin is a fat-derived cytokine with anti-diabetic and anti-atherogenic properties. In this study, effects of sulfonylureas (SUs) on adiponectin production and the action mechanism were evaluated using 3T3-L1 adipocytes. The cells were incubated with glimepiride, glibenclamide, gliclazide, pioglitazone, metformin and the medium only as the co...

Type 2 diabetic patients previously treated with rapid-acting insulinotropic agent (glinide) or a sulfonylurea other than gliclazide, were treated with low-dose gliclazide (less than 40 mg, daily dose) for 12 months to counter poor glycemic control or hypoglycemic events, and efficacy was evaluated. Subjects were 13 randomly selected patients previ...

A necessary condition of advocated criteria to determine the metabolic syndrome (MetS) in Japan is waist circumference (WC), which varies among races. In this study, we measured WC and visceral fat area (VFA) in subjects with type 2 diabetes (T2DM) and assessed the propriety of new criteria of MetS in Japan. Four hundred and nineteen patients (M/F:...

To compare the pharmacokinetic and pharmacodynamic effects of glimepiride between once- and twice-daily dosing in type 2 diabetic patients. Eight Japanese type 2 diabetic patients, who had been treated with 2 mg glimepiride alone over 4 weeks (age 40-70, body mass index <or=25 kg/m2, hemoglobin A 1C<8.0%), were randomly assigned to the crossover st...

Neuropathy is one of the typical features of chronic complications of diabetes mellitus. Recent analyses indicate that subjects with impaired glucose tolerance (IGT) already have disturbance of peripheral nerve function. To test the role of adipocytokines, that tend to be abnormal in IGT subjects, on diabetic neuropathy, we analyzed the relationshi...

Background Although cholesterol management reportedly reduces fatal and non-fatal coronary heart disease (CHD) events in subjects with or without evident atherosclerotic disease, it is still uncertain whether these benefits extend to Japanese. Methods and Results The study group comprised 8,009 subjects with mildly elevated total cholesterol who we...

Adipocytokines, products from adipose tissue, have biological activities on the vascular system, and may affect diabetic angiopathy. In this study, we assessed the relationship between adiposity and plasma adipocytokine levels, and investigated the clinical significance of adiposity and plasma adipocytokine levels on diabetic micro- and macroangiop...

The aim of this study was to investigate the effect of apolipoprotein (apo)E4 allele on plasma LDL cholesterol response to calorie-restricted diet therapy in type 2 diabetic patients. Twenty-four diabetic patients with the apoE3/3 genotype and 11 diabetic patients with the apoE4/3 genotype were recruited. Participants were hospitalized for calorie-...

In patients with type 2 diabetic mellitus treated with insulin, we studied the clinical parameters for predicting the possibility of insulin therapy withdrawal. Subjects were 83 patients undergoing intensive insulin therapy. Insulin therapy was withdrawn in 42 but not in 41. Age, BMI, diabetes duration, HbA1c, serum fasting CPR (s-CPR), 24 hour uri...

Serum amyloid A (SAA) and C-reactive protein (CRP) have been suggested to be involved in the process of coronary heart disease (CHD) and to be potential markers and/or predictors of CHD. Remnant-like lipoprotein particles (RLPs), which are regarded as atherogenic remnant lipoprotein, are reported to be increased in type 2 diabetic patients. We asse...

In normal New Zealand white rabbits, immunization with rabbit lung ACE (angiotensin converting enzyme) induced atherosclerotic retinal changes, and glomerular changes similar to those seen in diabetic nephropathy. Also, in genetically diabetogenic rats, immunization with the rabbit lung ACE induced diabetic nephropathy and retinopathy.

In a random sample of 200 patients with type 2 diabetes mellitus, immunoreactivities to ACE (angiotensin converting enzyme) were measured by ELISA. Immunoreactivities were positive for 129 (64.5%) patients, and were positive in 30 (83.3%) out of 36 patients in the early stage of clinical diabetic nephropathy. Serum ACE activity in rabbits immunized...

62歳の男性.角膜輪,冠動脈硬化症,鎖骨下動脈硬化症,腹部.下肢閉塞性動脈硬化症を呈した家族性低HDL血症と糖尿病を合併した症例を経験した. HDL-C28mg/dlであり,長男のHDL-Cも低値であった.アポAに異常を認めなかった.本患者のマクロファージを用いた細胞実験ではHDLのコレステロール引き抜き能は低下していた.マクロファージATP-binding cassette transporter A1 mRNAのエクソン4欠損という転写異常が認められた.

We previously showed that the apolipoprotein (apo) Eepsilon2 allele is associated with the progression of diabetic nephropathy. The aim of the present study is to further investigate the association between apo E genetic polymorphism, plasma lipid levels (particularly remnant lipoproteins), and diabetic nephropathy. One hundred fifty-eight patients...

We determined the prevalence of so-called diabetic foot by a detailed questionnaire given to diabetic patients and their attending physicians recruited from 7 hospitals in southern Okayama. Prevalence of symptoms suggestive of diabetic peripheral neuropathy was 36.5%, a prevalence that increased with the duration from diabetes diagnosis. Among all...

Allelic frequencies of a (CCTTT)(n) pentanucleotide repeat in the NOS2A promoter region were determined in a total of 1393 unrelated individuals from five specific population groups in four continents: Africa, Europe, Asia, and the Caribbean. There were highly significant differences in allele frequencies between the ethnically diverse populations....

The effects of the calcium channel blockers verapamil, diltiazem, prenylamine and nifedipine on the ACTH- and cAMP-mediated pathway for steroidogenesis in a suspension solution of isolated rat adrenal cells were studied. Steroidogenesis stimulated by ACTH (10(-10) M) or cAMP (10(-3) M) was not inhibited by nifedipine (10(-4) M), but was strongly in...

The case of a 66-year-old woman with acute onset IDDM is reported. In October 1993, she experienced headache and fever and visited a nearby hospital for treatment. However, her symptoms were not relieved, and two weeks later she experienced diabetic ketoacidosis and was admitted to Kawasaki Medical School Hospital. Her serum was positive for antinu...

A sensitive and specific assay method to determine serum angiotensin converting enzyme (ACE) was developed. Although hippuryl-his-leu is commonly used as substrate, Angiotensin I (AI) was the substrate used to measure ACE activity in this study. AI hydrolysis was measured by radioimmunoassay of angiotensin II. ACE activity was expressed as the amou...

Five healthy men were given 1.0 gr of metyrapone p.o. (MTP test) with and without 0.5 gr of 1- dopa p.o. (DOPA-MTP test) or 10 mg of metoclopramide p.o. (MTC-MTP test) and plasma 11-deoxycortisol, cortisol, ACTH and pregnenolone (only in MTP test) were determined before and hourly after the drug administrating for 6 hrs. In the MTP test, plasma 11-...

The contaminating Proinsulin-Like Immunoreactivity (PLI) of several insulin preparations recently on the market was determined directly and after gel filtration by porcine and bovine proinsulin-specific radioimmunoassay. Although gel filtration of 800 μg of Novo lente gelchromatographed insulin revealed neither porcine nor bovine Proinsulin-Like Co...