Michiel Kleinnijenhuis

• PhD
• PostDoc Position at University of Oxford

Gyrification of the human cerebral cortex allows for the surface expansion that accommodates many more cortical neurons in comparison to other mammals. For neuroimaging, however, it forms a feature that complicates analysis. For example, it has long been established that cortical layers do not occupy the same depth in gyri and sulci. Recently, in v...

One of the most prominent characteristics of the human neocortex is its laminated structure. The first person to observe this was Francesco Gennari in the second half the 18th century: in the middle of the depth of primary visual cortex, myelinated fibres are so abundant that he could observe them with bare eyes as a white line. Because of its sali...

Structural connectivity research in the human brain in vivo relies heavily on fiber tractography in diffusion-weighted MRI (DWI). The accurate mapping of white matter pathways would gain from images with a higher resolution than the typical ~ 2 mm isotropic DWI voxel size. Recently, high field gradient echo MRI (GE) has attracted considerable atten...

Background. This study reports on a new method for golf performance enhancement employing personalized real-life neurofeedback during golf putting.Method. Participants (n = 6) received an assessment and three real-life neurofeedback training sessions. In the assessment, a personal event-locked electroencephalographic (EEG) profile at FPz was determ...

In recent years, there has been a growing interest in white matter anatomy of the human brain. With advances in brain imaging techniques, the significance of white matter integrity for brain function has been demonstrated in various neurological and psychiatric disorders. As the demand for interpretation of clinical and imaging data on white matter...

Intravital microscopy (IVM) enables live imaging of animals at single-cell resolution, offering essential insights into cancer progression. This technique allows for the observation of single-cell behaviors within their natural 3D tissue environments, shedding light on how genetic and microenvironmental changes influence the complex dynamics of tum...

Intravital microscopy (IVM) enables live imaging of animals at single-cell level, offering essential insights into cancer progression. This technique allows for the observation of single-cell behaviors within their natural 3D tissue environments, shedding light on how genetic and microenvironmental changes influence the complex dynamics of tumors....

Intravital microscopy (IVM) enables live imaging of animals at single-cell level, offering essential insights into cancer progression. This technique allows for the observation of single-cell behaviors within their natural 3D tissue environments, shedding light on how genetic and microenvironmental changes influence the complex dynamics of tumors....

Synopsis Fluorescence-guided surgery (FGS) with molecular-targeted probes offers a valuable tool to guide tumor resection, however, identifying suitable tumor-specific probes can be challenging. To ease FGS probe screening, an organoid-based multi-spectral 3D imaging platform was developed. The platform allows testing of multiple FGS probes at onc...

Revealing the 3D composition of intact tissue specimens is essential for understanding cell and organ biology in health and disease. State-of-the-art 3D microscopy techniques aim to capture tissue volumes on an ever-increasing scale, while also retaining sufficient resolution for single-cell analysis. Furthermore, spatial profiling through multi-ma...

Despite advances in three-dimensional (3D) imaging, it remains challenging to profile all the cells within a large 3D tissue, including the morphology and organization of the many cell types present. Here, we introduce eight-color, multispectral, large-scale single-cell resolution 3D (mLSR-3D) imaging and image analysis software for the parallelize...

Purpose: Neuroscience methods working on widely different scales can complement and inform each other. At the macroscopic scale, magnetic resonance imaging methods that estimate microstructural measures have much to gain from ground truth validation and models based on accurate measurement of that microstructure. We present an approach to generate...

The combination of diffusion MRI (dMRI) with microscopy provides unique opportunities to study microstructural features of tissue, particularly when acquired in the same sample. Microscopy is frequently used to validate dMRI microstructure models, addressing the indirect nature of dMRI signals. Typically, these modalities are analysed separately, a...

Microscopic features (that is, microstructure) of axons affect neural circuit activity through characteristics such as conduction speed. To what extent axonal microstructure in white matter relates to functional connectivity (synchrony) between brain regions is largely unknown. Using MRI data in 11,354 subjects, we constructed multivariate models t...

The combination of diffusion MRI with microscopy provides unique opportunities to study microstructural features of tissue, particularly when acquired in the same sample. Microscopy is frequently used to validate diffusion MRI microstructure models, addressing the indirect nature of dMRI signals. Typically, these modalities are analysed separately,...

Constrained spherical deconvolution determines the orientation of white matter fibres from the diffusion MRI signal. To do so, the diffusion profile of a single fibre is estimated and assumed constant across the sample. However, the diffusion signal is dependent on microstructural properties such as axonal diameter, packing and myelination, which q...

Microscopic features (i.e., microstructure) of axons affect neural circuit activity through characteristics such as conduction speed. Deeper understanding of structure-function relationships and translating this into human neuroscience has been limited by the paucity of studies relating axonal microstructure in white matter pathways to functional c...

Fig. S1. DW‐STEAM sequence modification efficacy. Resultant b‐values for conventional and modified DW‐STEAM sequence according to the postmortem protocol are shown in the top row. All gradients were applied in the slice‐select direction with the b‐value from the diffusion gradient set to b diff = 3.5, b diff = 0.7 (fixed‐b 0) and b diff=0.0 ms·μm−2...

Diffusion MRI is an exquisitely sensitive probe of tissue microstructure, and is currently the only non-invasive measure of the brain's fibre architecture. As this technique becomes more sophisticated and microstructurally informative, there is increasing value in comparing diffusion MRI with microscopic imaging in the same tissue samples. This stu...

Purpose: To demonstrate how reference data affect the quantification of the apparent diffusion coefficient (ADC) in long diffusion time measurements with diffusion-weighted stimulated echo acquisition mode (DW-STEAM) measurements, and to present a modification to avoid contribution from crusher gradients in DW-STEAM. Methods: For DW-STEAM, refer...

Purpose: To investigate the effect of realistic microstructural geometry on the susceptibility-weighted MR signal in white matter (WM), with application to demyelination. Methods: Previous work has modeled susceptibility-weighted signals under the assumption that axons are cylindrical. In this study, we explored the implications of this assumpti...

Purpose: To investigate the effect of realistic microstructural geometry on the susceptibility-weighted magnetic resonance (MR) signal in white matter (WM), with application to demyelination. Methods: Previous work has modeled susceptibility-weighted signals under the assumption that axons are cylindrical. In this work, we explore the implications...

Diffusion-weighted imaging (DWI) tractography is a technique with great potential to characterize the in vivo anatomical position and integrity of white matter tracts. Tractography, however, remains an estimation of white matter tracts, and false-positive and false-negative rates are not available. The goal of the present study was to compare postm...

Target Audience: MRI community performing diffusion weighted imaging with high b-values and long diffusion times. Purpose: Diffusion weighted imaging (DWI) using very high b-values and long diffusion times (Δ) typically uses the stimulated echo (STE) in order that large diffusion encodings can be produced without incurring excessive T2-related sign...

Target Audience: MRI community performing diffusion weighted imaging with high b-values and long diffusion times. Purpose: Diffusion weighted imaging (DWI) using very high b-values and long diffusion times (Δ) typically uses the stimulated echo (STE) in order that large diffusion encodings can be produced without incurring excessive T2-related sign...

Introduction Conventional diffusion MRI provides exquisite sensitivity to tissue microstructure through models of restricted and hindered diffusion within and around axons, respectively. These models often idealize axons as parallel, infinite and impermeable cylinders, where diffusion is often assumed to be free along the direction of axons. Howeve...

Purpose: This work presents a validation of estimating dispersion anisotropy of neurites, using Bingham-NODDI [1]. Bingham-NODDI is a recent development of the diffusion MRI (d-MRI) technique called NODDI (neurite orientation dispersion and 3 density imaging) [2]. NODDI enables mapping of the morphology of neurites (axons and 0.7 dendrites) in the...

PURPOSE. Diffusion weighted imaging aims to unravel the microstructural properties of white matter in the brain by detecting alterations to diffusive motion along different orientations. Increasingly sophisticated biophysical models are used to estimate properties like fibre orientation dispersion in addition to mean orientation. Most models assume...

PURPOSE – Diffusion imaging at long diffusion times can inform on microstructural features of tissue at scales spanning several hundreds of micrometers. At these scales the common approximation of axons as straight cylinders might not hold, even for tissues that are generally assumed to be coherently organized. The human corpus callosum is such a t...

Preterm infants are born during a critical period of brain maturation, in which even subtle events can result in substantial behavioral, motor and cognitive deficits, as well as psychiatric diseases. Recent evidence shows that the main source for these devastating disabilities is not necessarily white matter (WM) damage but could also be disruption...

In these times where connectionist accounts of brain function are gaining in popularity, there is a need for reliable tools for determining anatomical connectivity in the living human brain. The technique of choice is diffusion MRI, but it is debatable whether this tool is suitable for mapping all but the major pathways. The thesis describes my con...

A 55-year-old female is presented with transient cerebellar mutism caused by a well-circumscribed left pontine infarction due to postoperative basilar perforator occlusion. Although conventional T2 imaging shows a well-demarcated lesion confined to the pontine region, diffusion tensor imaging shows an asymmetry in fractional anisotropy in the super...

Introduction Diffusion weighted imaging (DWI) has the potential to provide the richest noninvasive description of cortical cytoarchitecture. Previous work has shown that the diffusion properties of the primary visual cortex (V1) are layer‐specific (Kleinnijenhuis et al., 2012). In particular, the stria of Gennari displays low diffusivity and anisot...

Introduction Over the past two decades, diffusion weighted imaging (DWI) enabled detailed investigation of white matter (WM) microstructure. More recently, progress in advanced DWI methods (e.g. high-field DWI) is now allowing the cortical structure to be studied noninvasively [1]. This is a particularly exciting development, because DWI has the po...

In patients with Alzheimer's disease (AD) the severity of white matter degeneration correlates with the clinical symptoms of the disease. In this study, we performed diffusion-tensor magnetic resonance imaging at ultra-high field in a mouse model for AD (APP(swe)/PS1(dE9)) in combination with a voxel-based approach and tractography to detect change...

Probleemstelling Het leren van de witte stof anatomie van de humane hersenen is lastig omdat het complexe 3D netwerk van witte stof banen door de hele hersenen verspeid is in zowel de oppervlakkige als de diepe lagen van de hersenen. Door de recente ontwikkelingen in de neuroimaging met technieken zoals diffusion weighted imaging (DWI) en tractogra...

One of the most prominent characteristics of the human neocortex is its laminated structure. The first person to observe this was Francesco Gennari in the second half the 18th century: in the middle of the depth of primary visual cortex in the occipital lobes, myelinated fibres are so abundant that he could observe them with bare eyes as a white li...

Introduction Axonal membranes and myelin are known to be the main determinant of diffusion anisotropy in the brain [1]. Myelinated axons are present not only in white matter, but extend radially into the deeper cortical layers crossing with the tangentially oriented myelinated fibers also abundant in these layers [2]. Recently, several studies have...

Introduction. Slow Cortical Potential (SCP) neurofeedback and Galvanic SkinResponse (GSR) biofeedback training were used to investigate self-regulatory control over 10 central and peripheral arousal processes in two groups of healthy participants.Method. One group completed the SCP neurofeedback training procedure; the other group performed the GSR...

Introduction In diffusion tensor tractography (DTT), white matter structure is inferred in vivo by reconstructing fiber tracts from diffusion weighted images (DWI). Recently [1], white matter structure has also been shown at 7T using susceptibility weighted imaging (SWI) [2]. Most notably, SWI shows excellent contrast between the highly myelinated...

Background. Operant conditioning of one's slow cortical potential (SCP) or sensorimotor rhythm (SMR) can be used to control epilepsy or to manipulate external devices, as applied in BCI (Brain-Computer Interface). A commonly accepted view that both SCP and SMR are reflections of central arousal suggests a functional relationship between SCP and SMR...

Background Operant conditioning of one’s slow cortical potential (SCP) or sensorimotor rhythm (SMR) can be used to control epilepsy or to manipulate external devices, as applied in BCI (Brain-Computer Interface). To be practical, a BCI-system should use as less channels as possible. For this purpose, a wireless biofeedback system was developed that...

The present invention relates to an apparatus for measurement of a physiological function of a body comprising; physiological function detection means responsive to a physiological signal and capable of generating an analog output signal corresponding to the physiological signal; an analog-digital converter capable of receiving the unamplified anal...

Learning curves of volitional control of GSR and SCP after feedback will be presented, and compared to changes in simultaneously measured spontaneous SCP and GSR. Effects of training on QEEG profiles and neuropsychological functioning will be discussed. Both GSR and SCP have been associated with epilepsy. These measures may be different expressions...

Summary: The presentation will introduce a new method for improving golf performance. Some sports psychology/brain physiology backgrounds will be discussed as well as the assessment method and the promising results from the initial experiment. Abstract: This study reports on a very promising, new method for golf performance enhancement employing re...

In het Medisch Spectrum Twente te Enschede is op de afdeling klinische neurofysiologie nieuwe EEG- apparatuur aangeschaft. Met EEG-apparatuur kan het elektro-encefalogram opgenomen worden. Dit is een weergave van de extracraniële potentialen als gevolg van de activiteit van neuronen in de hersenen. Deze worden gemeten met elektroden die op de hoofd...